IndraLab

Statements

USP11 binds NONO. 15 / 15
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"USP11 interacts with NONO."
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"Taken together, these results suggest there is a direct interaction between USP11 and NONO in vivo."
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"Furthermore, we investigated the interaction of endogenous USP11 and NONO in melanoma cells SK‐Mel‐28 and A375 using co‐IP."
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"We demonstrated that USP11 interacted with NONO, removed poly‐ubiquitin chains conjugated onto NONO."
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"Based upon the observed interaction between NONO and USP11 described above, we speculate that USP11 may affect NONO levels in melanoma cells."
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"To analyse whether USP11 and NONO interacts with each other directly, purified recombinant USP11 protein was generated and GST‐pulldown assays results showed that recombinant GST‐USP11, but not the GST control, could bind to Myc‐NONO protein expressed in HEK293 cells (Figure  xref )."
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"Furthermore, we investigated the interaction of endogenous USP11 and NONO in melanoma cells SK-Mel-28 and A375 using co-IP."
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"Thus, our study shows that USP11‐NONO signalling axis plays a critical role in melanoma proliferation."
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"Previous studies have demonstrated that there is the possibility that USP11 and NONO may interact with each other. xref In order to ascertain the hypothesis, we transfected Flag‐USP11 and Myc‐NONO into HEK293 cells, collected total proteins and used the antibodies against Flag/Myc and the isotype‐matched control IgGs to perform coimmunoprecipitation (co‐IP)."
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"USP11 interacted with NONO and reversed its poly-ubiquitination."
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"We demonstrated that USP11 interacted with NONO, removed poly-ubiquitin chains conjugated onto NONO."
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"3.1 USP11 interacts with NONO."
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"USP11 interacted with NONO and reversed its poly‐ubiquitination."
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"Taken together, these results suggest there is a direct interaction between USP11 and NONO in vivo."
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"Collectively, these results demonstrate that USP11 is a new deubiquitinase of NONO and that the signalling axis of USP11‐NONO is significantly involved in melanoma proliferation."
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