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SDC4 binds FN1. 96 / 99
1 | 37 58
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"Strong expression of SDC4 or FN was associated with significantly shorter overall survival, respectively. [corrected] Increased expression of SDC4 and FN may be underlying molecular alteration of osteosarcoma which accounts for more aggressive clinical behaviour."
22531343
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"As an example, in endothelial cells, binding of syndecan-4 to fibronectin cated substrate results in syndecan-4 co-clustering with integrin alpha5beta1 and the activation of Rac-1 through the intracellular scaffold protein synectin."
19921267
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"We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a co-receptor complex in satellite cells and that binding of the glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells."
26136409
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"A recent study shows that the binding of fibronectin to syndecan-4 induces alpha5beta1-integrin endocytosis in a dynamin- and caveolin dependent manner [XREF_BIBR]."
22605996
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"This study aimed to explore the effects of TNF-α on the formation of FN/ITGβ1/SYND4 complex and the relative mechanism in NP cells."
31111299
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"TNC competes syndecan-4 binding to FN, thus blocking integrin α5β1-mediated cell adhesion and actin stress fiber formation ( xref ) which results in a pro-tumorigenic gene expression profile and repression of multiple cell adhesion associated genes ( xref )."
29259017
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"Syndecan-4 connects 2 important signaling pathways (proteoglycans in cancer and ECM–receptors interactions) and it has been reported that blocking interactions between syndecan-4 and fibronectin decreases focal adhesions in cells, leading to increased cell proliferation in tumors."
33693651
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"Both glypican and syndecan-4 bind the HepII domain of fibronectin similarly."
11544026
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"Because the Link module of TSG-6 binds avidly to glycosaminoglycans (e.g., heparan sulfate (Mahoney et al., 2005)), TSG-6 may facilitate FN fibrillogenesis either by directly engaging the glycosaminog[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18042364
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"Importantly, the binding of SDC4 to fibronectin is required by certain cell types for the activation of focal adhesion kinase (FAK), which stabilises focal adhesions by activating Rho and recruiting v[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26796346
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"Syndecan-4 binds FN, inducing the formation of focal adhesions and activation of Rho, Rac and protein kinase Calpha (PKCalpha), yet syndecan-4-depleted cells deposit FN."
24190885
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"Syndecan-4 is a proteoglycan that spans the cell membrane and can bind to fibronectin [ xref ]."
33353083
sparser
"A syndecan-4-dependent interaction of cells with fibronectin regulates PCP components in the chordamesoderm xref , xref ."
24355988
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"In focal adhesions, cadherin-11 co-localizes with beta1-integrin and paxillin and physically interacts with the fibronectin binding proteoglycan syndecan-4."
26952325
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"It interferes with syndecan-4 binding to fibronectin and enhances proliferation of glioma and breast cancer cells 57."
28469129
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"Furthermore, binding of Syndecan4 to fibronectin facilitates Fz7 recruitment of Dsh to the plasma membrane and, subsequently, the activation of noncanonical Wnt signaling."
17130287
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"Therefore, we conclude that Sdc4 binds FN on activated satellite cells."
23290138
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"Since tenascin-C competed with syndecan-4 binding to fibronectin, it is possible that syndecan-4 is exclusively available as a coreceptor for growth factor receptors in the presence of tenascin-C. In summary, in addition to its role in regulating the inflammatory response, there is strong evidence that tenascin-C may also play a direct role in the control of growth factor signaling that promotes ECM synthesis."
19838819
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"This binding of syndecan-4 to FN and its association with the cytoskeleton may be relevant for syndecan-4-dependent dendritic cell motility."
17576238
sparser
"Furthermore, FN1 interacts with SDC4, an ECM protein that was decreased in abundance, and with the enzymes CP and CTSD, both increased in abundance in the T1D dataset."
28819457
sparser
"Therefore, we conclude that Sdc4 binds FN on activated satellite cells."
23290138
sparser
"For example, the focal adhesion component syndecan-4 interacts with the III(12-14) region of fibronectin (HepII domain) through its HS chains."
17130131
sparser
"This indicates that tenascin-C causes cell cycle arrest and cdk2 inactivation by interfering with fibronectin-syndecan-4 interactions."
12813465
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"Once again, cells were able to spread without PKCα engagement; however, for focal complexes to form, interaction of syndecan-4 with fibronectin to recruit PKCα-mediated Rac1 activation was required, as knock down or drug-mediated inhibition of this kinase failed to activate Rac1 and induce the maturation of focal complexes ( xref )."
24309511
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"Syndecan-4 interacts with the Hep II domain of FN through heparin sulfate chains with specific properties xref , and activates protein kinase C-α signalling xref in the presence of inositol phospholipids (reviewed xref , xref )."
30237127
sparser
"In both cases, tenascin-C acts by blocking the interaction of fibronectin with cell-surface syndecan-4 (Huang et al. xref ; Midwood et al. xref ) by binding directly to FNIII13 in the HepII domain of fibronectin (Huang et al. xref )."
19838819
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"We find that although Rac1, RhoA, RhoG and Arf6 are each regulated by the interaction between syndecan-4 and fibronectin, none of them is directly dependent on the others in the fibronectin signaling pathway."
22790193
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"In these tumors, TNC reduces cell spreading on FN interfering with FN binding to the integrin co-receptor syndecan-4, thus compromising focal contact and stress fiber formation xref ."
21747918
sparser
"Disruption of interactions between syndecan-4 and fibronectin by the extracellular matrix molecule tenascin-C decreases focal adhesions on tumor cells leading to increased tumor cell proliferation [ xref ]."
21155971
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"Disruption of interactions between syndecan-4 and fibronectin by the extracellular matrix molecule tenascin-C decreases focal adhesions on tumor cells leading to increased tumor cell proliferation [XREF_BIBR]."
21155971
sparser
"This binding of syndecan-4 to FN and its association with the cytoskeleton may be relevant for syndecan-4-dependent dendritic cell motility."
17576238
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"Following Sdc4 binding to FN in the extracellular matrix, the Sdc4 cytoplasmic domain binds phosphatidylinositol 4,5-bisphosphate, which stimulates PKCalpha activation, leading to the activation of small GTPases and assembly of focal adhesions (Oh et al., 1997; Saoncella et al., 1999; Couchman, 2003; Lim et al., 2003; Dovas et al., 2006)."
23290138
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"Tenascin-c, an extracellular matrix protein, is highly expressed in most solid tumors and antagonizes the adhesion of tumor cells through competitive binding to the syndecan-4 binding site on fibronec[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12781370
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"Syndecan-4 (Syn-4) binds fibronectin and together with integrins stabilizes FA sites xref ."
26952325
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"Comparison of the dissociation rate extracted for the SDC4-fibronectin interaction in this study reveals a 12-fold increase when compared with that reported for the heparin-fibronectin ( xref ), suggesting a weaker interaction between fibronectin and SDC4 than heparin."
31337547
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"One mechanism by which TNC modulates cell adhesion dependent processes involves its direct interaction with FN, which leads to interference of FN binding to syndecan-4 12."
28986537
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"Syndecan-4 (Sdc4) and Fzd7 form a co-receptor complex in satellite cells and binding of the ECM glycoprotein FN to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells."
26453496
sparser
"Previously, we had shown that cells are inhibited by TNC to spread on a combined FN/TNC substratum since TNC competed syndecan-4 binding to FN (44, 69)."
33790905
sparser
"Additionally, in fibroblasts, the binding of Syndecan-4 to fibronectin allows the association of Syndecan-4 with protein kinase C-alpha (PKCα), which activates RhoG and triggers endocytosis of β integ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27235833
sparser
"Furthermore, binding of Syndecan4 to fibronectin facilitates Fz7 recruitment of Dsh to the plasma membrane and, subsequently, the activation of noncanonical Wnt signaling ( xref ; xref )."
17130287
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"We used PLA to assess the binding of FN to Sdc4 in satellite cells, and similarly detected a strong signal in satellite cells on cultured myofibers (XREF_FIG)."
23290138
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"Syndecan-4 glycosaminoglycan chains bind the HepII domain of FN1 in the proximity of alpha5beta1 integrin binding domain, and is included into nascent adhesions, suggesting that it participates in cell-FN1 interaction [XREF_BIBR]."
33731188
sparser
"Disruption of interactions between syndecan-4 and fibronectin by the extracellular matrix molecule tenascin-C decreases focal adhesions on tumour cells leading to increased tumour cell proliferation [ xref ]."
21155971
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"As an example, in endothelial cells, binding of syndecan-4 to fibronectin-cated substrate results in syndecan-4 co-clustering with integrin α5β1 and the activation of Rac-1 through the intracellular scaffold protein synectin (Tkachenko et al. xref ; Morgan et al. xref )."
19921267
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"In normal glucose conditions, small TN-C isoforms bind to FN and are incorporated into the matrix where they can limit the degree of FN matrix assembly in the glomerulus by controlling FN-syndecan-4 interactions, cell signaling, and contractility."
33543041
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"Syndecan-4 also provides additional signalling for focal adhesion formation by binding directly to fibronectin itself primarily via the HepII domain (containing the fibronectin type-III repeats 12-14) in the C-terminal region."
22607334
sparser
"For example, recruitment of Dsh (a key intracellular PCP component) to the cell membrane is dependent on fibronectin–integrin and Sdc4–fibronectin interactions in Xenopus CE ( xref )."
34842271
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"In tetrapods (amphibians, reptiles, birds and mammals) there are 4 tenascin genes: tenascin-C, tenascin-R, tenascin-W and tenascin-X ( xref ). xref Tenascin-C is the original “tenascin” that was first described independently in the myotendinous junctions and brains of the chicken Gallus gallus xref as well as in human glioblastoma-derived cells. xref The best studied of the tenascins, tenascin-C is expressed by motile cells such as the neural crest, at sites of connective tissue differentiation and in the adult in numerous stem cell niches; it also reappears during inflammation, at the margins of healing wounds and in the stroma of solid tumors. xref Tenascin-C from G. gallus binds to α9β1 integrins via the isoleucine-aspartic acid-glycine (IDG) motif found in the B-C loop of its third FNIII domain xref and to αVβ3 integrins via an RGD motif in the F-G loop of the same FNIII domain. xref Tenascin-C can also bind to fibronectin and prevent syndecan-4-fibronectin interactions, which in turn influences fibronectin signaling through integrins. xref Tenascin-C knockout mice have profound behavioral abnormalities and other phenotypes that are primarily associated with aberrant stem cell migration, proliferation and differentiation. xref Tenascin-R, the second tenascin to be discovered, is restricted in its expression to the nervous system xref and tenascin-R knockout mice also display abnormal behavior. xref Tenascin-W is often co-expressed with tenascin-C both in development and in stem cell niches. xref Murine tenascin-W has an RGD motif in the F-G loop of the second FNIII domain that appears to act as an α8β1 integrin-binding site. xref It and the tenascin-W of other species have the related lysine-glycine-aspartic acid (KGD) motif in the F-G loop of one or more FNIII domains, and synthetic peptides that contain this motif and neighboring conserved sequences promote cell proliferation. xref Tenascin-W knockout mice have not been analyzed."
25482621
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"Bentzinger et al. showed that fibronectin could bind to Syndecan-4 to promote the expression of Wnt7a, thereby inducing the symmetrical division of satellite cells, and activated satellite cells can also reconstruct niche by autocrine fibronectin [XREF_BIBR]."
| PMC
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"We used PLA to assess the binding of FN to Sdc4 in satellite cells, and similarly detected a strong signal in satellite cells on cultured myofibers ( xref )."
23290138
sparser
"As a sensor to the ECM mechanical properties, syndecan-4 binds to fibronectin to activate a series of signals, including the MAPK pathways, leading to cell proliferation and migration [ xref ]."
| PMC
sparser
"We find that although Rac1, RhoA, RhoG and Arf6 are each regulated by the interaction between syndecan-4 and fibronectin, none of them is directly dependent on the others in the fibronectin-signaling pathway."
22790193
sparser
"We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a co-receptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells."
23290138
sparser
"Syndecan-4 interaction with TG2-FN complex was suggested to lead to β1 integrins activation via PKCα, with subsequent integrin mediated activation of the FAK downstream signalling cascade in RGD-independent cell adhesion [ xref ]."
30621228
signor
"Sdc4 is a high affinity receptor for fibronectin (FN) […] Therefore, we conclude that Sdc4 binds FN on activated satellite cells."
23290138
reach
"In particular, syndecan-4 binds the major heparin binding site of fibronectin and acts coordinately with integrins such as alpha5beta1 in the assembly of focal adhesions (Woods et al. 1993; Couchman and Woods 1999; Longley et al. 1999; Saoncella et al. 1999)."
11257118
sparser
"The integrin α5β1 heterodimer ( xref ) and Sdc4 ( xref ; xref ) bind to distinct domains of fibronectin and are required for NC cell adhesion and migration."
27101845
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"Following Sdc4 binding to FN in the extracellular matrix, the Sdc4 cytoplasmic domain binds phosphatidylinositol 4,5-bisphosphate, which stimulates PKCα activation, leading to the activation of small GTPases and assembly of focal adhesions (Oh et al., 1997; Saoncella et al., 1999; Couchman, 2003; Lim et al., 2003; Dovas et al., 2006)."
23290138
sparser
"Furthermore, the width of the energy barrier probed in this work for the SDC4 interaction with fibronectin is only ∼10% larger (and falls within the range of associated errors) than the width of 3.1 ± 0.1 Å reported for heparin ( xref )."
31337547
reach
"Previously, we had shown that cells are inhibited by TNC to spread on a combined FN/TNC substratum since TNC competed syndecan-4 binding to FN."
33790905
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"It can be noted that both the width of the barrier and the dissociation rate extracted from the dynamic force spectra for the SDC4-fibronectin interaction are comparable to values reported for other protein-carbohydrate interactions ( xref , xref )."
31337547
reach
"Rather, a domain of fibronectin that binds to syndecan-4 is also required for induction of these structures, and antibodies to syndecan-4 can substitute."
10916159
reach
"We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a co and receptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells."
23290138
sparser
"It interferes with syndecan-4 binding to fibronectin and enhances proliferation of glioma and breast cancer cells xref ."
28469129
reach
"Since tenascin-C competed with syndecan-4 binding to fibronectin, it is possible that syndecan-4 is exclusively available as a coreceptor for growth factor receptors in the presence of tenascin-C."
19838819
reach
"XREF_BIBR TNC binding inhibits the interaction between fibronectin and syndecan-4 (SDC4), a transmembrane heparan sulfate proteoglycan that is required for cell spreading on fibronectin in synergy with integrin alpha5beta1."
25738825
sparser
"Previously, we had shown that cells are inhibited by TNC to spread on a combined FN/TNC substratum since TNC competed syndecan-4 binding to FN ( xref , xref )."
33790905
sparser
"Rather, a domain of fibronectin that binds to syndecan-4 is also required for induction of these structures, and antibodies to syndecan-4 can substitute."
10916159
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"Fibronectin binding to the Syndecan4 and Frizzled complex disrupts the asymmetric self-renewal of MuSCs, thereby promoting Wnt7a mediated symmetrical muscle stem cell expansion 100."
26248268
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"Mechanistically, the C-terminal region of tenascin-C recognizes the heparin-binding site in the 13th FNIII domain of fibronectin, thereby blocking the interaction of fibronectin with the cell surface proteoglycan syndecan-4 [ xref ]."
23313574
reach
"Furthermore, FN1 interacts with SDC4, an ECM protein that was decreased in abundance, and with the enzymes CP and CTSD, both increased in abundance in the T1D dataset."
28819457
sparser
"Syndecan-4 binds FN, inducing the formation of focal adhesions ( xref ; xref ) and activation of Rho, Rac and protein kinase Cα (PKCα) ( xref ; xref ), yet syndecan-4-depleted cells deposit FN ( xref )."
24190885
sparser
"Consistent with this, while fibronectin binds to syndecan 4 and modulates frizzled-mediated Wnt response to maintain SCs in their niche xref , laminin-α1 mediates its effect on SC self-renewal through integrin-α6β1."
29540680
sparser
"A recent study shows that the binding of fibronectin to syndecan-4 induces α5β1-integrin endocytosis in a dynamin- and caveolin-dependent manner [ xref ]."
22605996
reach
"Interference of tenascin-C with syndecan-4 binding to fibronectin blocks cell adhesion and stimulates tumor cell proliferation."
11731446
sparser
"Specifically, syndecan-4 directly binds fibronectin to promote cell adhesions, thereby affecting cell migration, whereas the syndecan-4/PKCα/RhoA signaling axis promotes focal adhesion formation ( xref ; xref )."
33178691
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"Thus, the potential heparin antimetastatic activity can be based on interference either directly with the integrin-fibronectin binding or indirectly via competing for the proteoglycan binding of syndecan-4 to fibronectin."
22505933
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"Syndecan-4 (Syn-4) binds fibronectin and together with integrins stabilizes FA sites XREF_BIBR."
26952325
sparser
"Thus, Telci et al. [ xref ] have shown that interaction of fibronectin with a cell surface receptor Syndecan-4 can restore adhesion of fibroblast even if direct interaction between fibronectin and the cells is blocked."
26351461
sparser
"The SDC4-α5β1 integrin mediated cell adhesion to fibronectin reduces tumor cell proliferation, whilst the tenascin-C-mediated inhibition of SDC4-fibronectin interaction and consequently the impaired fibronectin-induced signaling enhances the proliferation of glioblastoma cells [ xref ]."
34282767
reach
"Additionally, in fibroblasts, the binding of Syndecan-4 to fibronectin allows the association of Syndecan-4 with protein kinase C-alpha (PKCalpha), which activates RhoG and triggers endocytosis of bet[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27235833
sparser
"Orend et al. [ xref ] demonstrated that TNC causes cdk2 inactivation and blocks cell cycle progression from G 1 phase to S phase of anchorage-dependent fibroblasts by interfering with fibronectin–syndecan-4 interactions."
32817625
reach
"Importantly, the binding of SDC4 to fibronectin is required by certain cell types for the activation of focal adhesion kinase (FAK), which stabilises focal adhesions by activating Rho and recruiting v[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26796346
sparser
"The PCP protein, Disheveled, is translocated to the plasma membrane [ xref ] by a FN-dependent interaction of syndecan-4 with frizzled7 [ xref ]."
20739170
sparser
"TN-C has been reported to compete with the binding site of fibronectin with syndecan-4, and this interaction with syndecan-4 partially abrogates the effects of this co-receptor, as well as attenuates the interaction of syndecan-4 with fibronectin, enhancing tumor cell malignancy."
35246056
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"The PCP protein, Disheveled, is translocated to the plasma membrane [XREF_BIBR] by a FN dependent interaction of syndecan-4 with frizzled7 [XREF_BIBR]."
20739170
reach
"Among ECM proteins, fibulin-1 was reported to interact with fibronectin and inhibit cell migration by disrupting the association between fibronectin and syndecan-4, which played positive roles in cell migration XREF_BIBR."
27194945
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"Moreover, blocking of FN binding to Sdc4 with Tenascin-C (TEN) selectively impaired the ability of Wnt7a to stimulate the expansion of the Pax7 + / YFP - satellite stem cell pool on myofibers cultured for 42h (XREF_FIG) without any effect on Pax7 + / YFP + satellite myogenic cells (XREF_SUPPLEMENTARY)."
23290138
sparser
"Furthermore, Wnt signaling, which dictates the fate of satellite cells by controlling their expansion, is highly dependent on the binding of the ECM glycoprotein fibronectin to syndecan-4 [ xref ]."
25729564
sparser
"In this system, FN binding to Syndecan 4 stimulates it to form a co-receptor with Frizzled 7 that supports downstream signaling via Wnt7a to increase satellite cell expansion within the niche [ xref ]."
23849799
reach
"Disruption of interactions between syndecan-4 and fibronectin by the extracellular matrix molecule tenascin-C decreases focal adhesions on tumour cells leading to increased tumour cell proliferation [XREF_BIBR]."
21155971
sparser
"Because the Link module of TSG-6 binds avidly to glycosaminoglycans (e.g., heparan sulfate ( Mahoney et al., 2005 )), TSG-6 may facilitate FN fibrillogenesis either by directly engaging the glycosamin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18042364
reach
"Importantly, the binding of SDC4 to fibronectin is required by certain cell types for the activation of focal adhesion kinase (FAK), which stabilises focal adhesions by activating Rho and recruiting vinculin and paxillin."
26796346
sparser
"Ruiz and colleagues [ xref ] and Lange and colleagues [ xref ] have associated triggering of glioma cell migration with a simultaneous mechanism of competitive inhibition of syndecan-4 binding of fibronectin and signalling by lysophosphatidic acid and platelet-derived growth factor (reviewed in [ xref ])."
20678196
sparser
"Bentzinger et al. showed that fibronectin could bind to Syndecan-4 to promote the expression of Wnt7a, thereby inducing the symmetrical division of satellite cells, and activated satellite cells can also reconstruct niche by autocrine fibronectin [ xref ]."
| PMC
reach
"We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a coreceptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells."
23290138