IndraLab
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"Abstract Coronaviruses encode papain-like proteases (PLpro) that are often multifunctional enzymes with protease activity to process the viral replicase polyprotein and deubiquitinating (DUB)/deISGylating activity, which is hypothesized to modify the innate immune response to infection."
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"It is known that PLpro exhibits deubiquitinating (DUB) activity and antagonizes the induction of type-1 interferon (IFN), the interferon-stimulated gene 15 (ISG15) is the most overexpressed gene upon IFN stimulation and it’s involved in marking newly synthesised protein during an antiviral response."
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"6, 7 On the basis of this similarity in molecular architecture and the predicted specificity of PLpro for cleavage after a diglycine residue, Sulea and co-workers predicted that PLpro has deubiquitinating (DUB) activity.To test this prediction we used our experimentally determined catalytic core domain in an in vitro assay for deubiquitination."