IndraLab

Statements

CYLD deubiquitinates TP53. 9 / 9
1 | 8
ubibrowser
"Mechanistically, CYLD interacts with and deubiquitinates p53 facilitating its stabilization in response to genotoxic stress.| Collectively, our results identify CYLD as a deubiquitinase facilitating DNA damage-induced p53 activation and suggest that regulation of p53 responses to genotoxic stress contributes to the tumour suppressor function of CYLD."
27561390
reach
"As shown in XREF_FIG and XREF_SUPPLEMENTARY, in contrast to WT CYLD, the catalytically inactive CYLDR936X (R/X) and CYLDH871N (H/N) mutants did not diminish p53 ubiquitination although they bound p53, demonstrating that CYLD DUB activity is required to reduce p53 ubiquitination."
27561390
reach
"To address this question, we first assessed the capacity of CYLD to reduce p53 ubiquitination in cells overexpressing HA tagged Lys-to-Arg ubiquitin mutants that can only form K48- or K63 linked chains."
27561390
reach
"Furthermore, recombinant His CYLD reduced ubiquitination of Flag-p53 immunoprecipitated from CpT treated HEK293T cells, showing that CYLD can directly deubiquitinate p53 in a cell-free in vitro assay (XREF_SUPPLEMENTARY)."
27561390
reach
"CYLD deubiquitinates p53 facilitating its stabilization."
27561390
reach
"Together, these results suggested that CYLD directly interacts with and deubiquitinates p53 facilitating its optimal stabilization in response to DNA damage."
27561390
reach
"Mechanistically, we show that CYLD interacts with and deubiquitinates p53 in response to DNA damage."
27561390
reach
"This experiment showed that CYLD diminishes p53 ubiquitination in cells expressing HA-mutant ubiquitin forming K63 only chains, but also in cells expressing HA-mutant ubiquitin forming K48 only chains (XREF_FIG)."
27561390
reach
"Mechanistically, CYLD interacts with and deubiquitinates p53 facilitating its stabilization in response to genotoxic stress."
27561390