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DUSP6 dephosphorylates MAPK1. 25 / 27
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"Furthermore, MKP3 is capable of efficiently dephosphorylating both the pTyr and the pThr on ERK2."
12191622
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"Expression of LINC01503 in ESCC cell lines reduced ERK2 dephosphorylation by DUSP6, leading to activation of ERK signaling via MAPK."
29454790
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"Thus, the molecular basis for efficient dual dephosphorylation of ERK2 and pTpY by MKP3 may lie in the specific interactions between ERK2 and MKP3 that are not possible between nonspecific substrates [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12191622
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"On the contrary, the knockdown of DUSP6 by si-RNA significantly enhanced the phosphorylation of ERK2 ( Figure 5D )."
35458535
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"On the other hand, when active MAPK1 was dephosphorylated by the exogenous expression of DUSP6, promoter activity was reduced."
18848526
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"We note that MKP-3 preferentially dephosphorylates ERK2, and in addition to the resolved structures of the catalytic domain and BD, a structure of ERK2 complexed with the kinase interaction motif from MKP-3 BD has been determined [ xref ]."
18855677
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"Our results also suggest that MKP3 can dephosphorylate ERK2 in cells in the absence of its N-terminal MAPK-binding domain."
22504224
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"The same study demonstrated that recombinant ERK2 can induce catalytic activation of DUSP6, and DUSP6 expression can de-phosphorylate a co-expressed ERK2 construct and block the MEK1 driven activation of GAL4-ELK1, an ERK1/2 regulated transcription factor."
29545965
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"ERK2 dephosphorylation by MKP3 has been shown to proceed by a mechanism whereby MKP3 binds to ERK2/pTpY and dephosphorylates ERK2/pY first, then dissociates and releases the monophosphorylated ERK2/pT[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12408986
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"ERK2 dephosphorylation by MKP3 has been shown to proceed by a mechanism whereby MKP3 binds to ERK2 and pTpY and dephosphorylates ERK2/pY first, then dissociates and releases the monophosphorylated ERK[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12408986
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"Nevertheless, MKP3 full length displayed more efficiency in the dephosphorylation of ERK2 than MKP3 153-381, in agreement with the notion that the N-terminal MAPK binding domain plays a major role in [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22504224
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"P-erk1/2 proteins were efficiently dephosphorylated in vitro by protein phosphatases 1 and 2a (pp1/2a) and mapk phosphatase 3 (mkp3)."
12840032
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"The dephosphorylation of ERK2 MAPK by Pyst1 (MKP-3) in mammalian cells is accompanied by the formation of a tight physical complex between the phosphatase and ERK2 [10]."
10712927
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"This observation is consistent with ERK2 dephosphorylation by the cytoplasmic MKP-3."
16288922
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"Recombinant Dusp6 completely dephosphorylated pERK2 in vitro as determined by immunoblotting with pERK specific antibodies ( xref , lane 3 )."
19578332
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"Importantly, LINC01503 bound directly to ERK2 and prevented the dephosphorylation of ERK2 by DUSP-6."
29454790
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"Our results also suggest that MKP3 can dephosphorylate ERK2 in cells in the absence of its N-terminal MAPK binding domain."
22504224
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"Importantly, LINC01503 bound directly to ERK2 and prevented the dephosphorylation of ERK2 by DUSP-6."
29454790
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"In addition to the mechanism of ERK2 dephosphorylation by MKP3, we also investigated the molecular basis of MKP3 substrate specificity."
12191622
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"We reasoned that if MKP3 dephosphorylates the bisphosphorylated ERK2 via a distributive mechanism, monophosphorylated ERK2 should be formed in excess of the MKP3 concentration in the reaction mixture."
12191622
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"We note that MKP-3 preferentially dephosphorylates ERK2, and in addition to the resolved structures of the catalytic domain and BD, a structure of ERK2 complexed with the kinase interaction motif from MKP-3 BD has been determined [XREF_BIBR]."
18855677
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"We find that ERK2 and pTpY dephosphorylation by MKP3 involves an ordered, distributive mechanism in which MKP3 binds the bisphosphorylated ERK2 and pTpY, dephosphorylates Tyr (P) first, dissociates and releases the monophosphorylated ERK2/pT, which is then subjected to dephosphorylation by a second MKP3, yielding the fully dephosphorylated ERK2."
11432864
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"The type II, DFG-out ERK2 inhibitor decreased the rate of dephosphorylation of ERK2 by MKP3, whereas type I DFG-in inhibitors enhanced dephosphorylation ( xref )."
26745528
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"As a result, compared to that of controls, the overexpression of DUSP6 significantly decreased the phosphorylation of ERK2, and inhibited the secreted viral protein level (p27) in the culture supernatants ( Figure 5A )."
35458535
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"When expressed in COS-7 cells, MKP-3 blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens."
8626780