A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.



phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach

GST binds TP53 and MDM2. 6 / 6
| 6
"As was observed using the cell extracts ( xref ), TAFII250 stimulated the interaction between wild-type p53 and GST-Mdm2 in pull-down experiments ( xref )."
"The amount of p53 bound to GST-MDM2 was also enhanced by the addition of PA28γ in an in vitro pull-down assay ( xref )."
"Thus starting with the wild-type p53 12mer sequence, affinity was increased by >1700-fold and the optimised peptide (Fig.  xref ) inhibited full-length p53 binding to GST-HDM2 with an IC 50 of 5 nM (García-Echeverría et al., xref )."
"Confirming this hypothesis, addition of specific HDM2 inhibitor nutlin xref disrupted the interaction between p53 and GST-HDM2, but did not influence the binding of LZAP to p53 in the same reaction ( xref )."
"Phage particles displaying p53 interacted with GST-MDM2 immobilized on 96-well plates, and the interaction was inhibited by nutlin 3."
"These combined modifications resulted in a peptide that inhibited binding of p53 to HDM2-GST with IC 50 = 5 nM for representing a 1700-fold improvement in overall binding affinity."