IndraLab

Statements



reach
"Consequently, under the present experimental condition, the concentration of DTT activated SLO above 28.5 mug/ml was necessary for evaluating the extent of pore formation by SLO in a lipid bilayer.Pre[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"When cholesterol oxidase and DTT activated SLO were added sequentially and P t was measured immediately, the time courses of nanopore formation of SLO in the presence and absence of cholesterol oxidas[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Consequently, for monitoring the progress of cholesterol oxidation, cholesterol oxidase was added first and incubated for a given time, and then a DTT activated SLO solution was added.The effect of co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"The mixture hereafter is called a DTT activated SLO solution.Each of the liposome immobilized wells (vide supra) contained 20 mul of a PB solution."

sparser
"These results indicate that the release of calcein occurred through DTT-activated SLO, i.e., the activation of SLO by DTT is necessary for eliciting the calcein permeation, and the perturbation of lip[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"When SLO was activated by DTT treatment, calcein permeability (P t) exhibited a rapid rise till 1 min and reached plateau after 5 min."

sparser
"After immobilization of liposomes onto a 384-microplate, the progress of cholesterol hydroxylation in the lipid bilayers of cholesterol and PC (1:1, mol/mol) in the presence of cholesterol oxidase (28[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"After the incubation of the well for a given time, a 2 mul portion of a DTT activated SLO solution (0.2 mg/ml) was added."

sparser
"By adding DTT-activated SLO, the calcein permeability gradually increased with time and reached plateau after 30 min (Fig. S2)."

reach
"After immobilization of liposomes onto a 384-microplate, the progress of cholesterol hydroxylation in the lipid bilayers of cholesterol and PC (1:1, mol and mol) in the presence of cholesterol oxidase[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"When cells were perfused with DTT-activated SLO (in PBS) a similar change in impedance magnitude was recorded (Fig.  xref )."

reach
"When cells were perfused with DTT activated SLO (in PBS) a similar change in impedance magnitude was recorded."

sparser
"Consequently, under the present experimental condition, the concentration of DTT-activated SLO above 28.5 μg/ml was necessary for evaluating the extent of pore formation by SLO in a lipid bilayer."

reach
"Glutathione and application of DTT increased BK channel open probability."

sparser
"When cholesterol oxidase and DTT-activated SLO were added sequentially and P t was measured immediately, the time courses of nanopore formation of SLO in the presence and absence of cholesterol oxida[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"These results indicate that the release of calcein occurred through DTT activated SLO, i.e., the activation of SLO by DTT is necessary for eliciting the calcein permeation, and the perturbation of lip[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"By adding DTT activated SLO, the calcein permeability gradually increased with time and reached plateau after 30 min."