IndraLab

Statements

USP49 activates APOBEC3G. 6 / 6
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"USP49 enhances the expression of A3G even in absence of Vif ."
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"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus.These data indicated that the anti-A3G effect of Vif can be enforced by depletion of the A3G protein stabilizer USP49 , alternatively supporting the fact that USP49 enhances the anti-HIV-1 effect of A3G ."
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"USP49 enhanced A3G levels in both cell lysates and viral particles ( Figure 3D ) ."
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"This result verifies that the inhibition of USP49 can promote the degradation of A3G and subsequently reduce the hypermutation in virus ."
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"USP49 directly interacts with A3G and deubiquitinates the K48-linked ubiquitination of A3G mediated by vif Considering that USP49 could enhance the expression of A3G directly , we next assessed whether USP49 binds A3G ."
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"USP49 enhances the expression of A3G even in the absence of HIV-1 vif Unexpectedly , in the absence of Vif , USP49 knockdown with siRNA also significantly decreased A3G stability , which could be rescued by MG132 ( Figure 3A , B ) , suggesting that A3G could be degraded through a Vif-independent pathway ."
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