IndraLab

Statements

USP11 binds Flag. 7 / 7
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"To test our hypothesis, we performed exogenous reciprocal Co-IP assays by co-transfecting Flag-USP11 and Myc-VCP into HEK293T cells."
33758608
sparser
"The results showed that Myc‐NONO was found in Flag‐USP11 precipitations but not present in IgG, and vice versa (Figure  xref )."
33369124
sparser
"Similarly, Flag-USP11 was also precipitated using an anti-Myc antibody (Figure xref A)."
33758608
sparser
"Previous studies have demonstrated that there is the possibility that USP11 and NONO may interact with each other. xref In order to ascertain the hypothesis, we transfected Flag‐USP11 and Myc‐NONO into HEK293 cells, collected total proteins and used the antibodies against Flag/Myc and the isotype‐matched control IgGs to perform coimmunoprecipitation (co‐IP)."
33369124
sparser
"The results showed that Myc-VCP was precipitated with Flag-USP11 using an anti-Flag antibody."
33758608
sparser
"To elucidate the underlying mechanism by which USP11 regulates the protein levels of NONO, Flag‐USP11 plasmid or control vector was introduced into SK‐Mel‐28 and A375, after 24 h, MG132 was added into the medium to inhibit the proteasome activity followed by the NONO examination."
33369124
sparser
"The NP-HA plasmid was replaced by PA-HA or PB2-HA, and visualized by immunoblot with anti-HA and anti-myc antibodies. (D) 293T cells were co-transfected with plasmids that can express NP-HA, Flag-USP10, Flag-USP11 and Myc-Ub as indicated."
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