IndraLab

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Tubulin binds KCNK18. 25 / 25
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"We provided evidence suggesting that the PKA dependent docking of 14-3-3 interfered with the binding of tubulin to TRESK."
24830385
sparser
"Although tubulin binds to TRESK in vitro , it remains to be established whether the two proteins also interact in the living cell."
24830385
sparser
"Therefore, we initially tested the binding of tubulin to TRESK loop, if this serine was or was not phosphorylated with protein kinase A before the usual pull-down assay."
24830385
sparser
"Our results also hint at the possibility that the interaction between TRESK and tubulin is conditional."
24830385
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"The cytoplasmic loop of the distantly related Danio rerio (zebrafish) TRESK (amino acids 174-284) also binds tubulin (as a GST-fusion protein, XREF_FIG, compare lane 3 to lanes 1 and 2)."
24830385
reach
"Although tubulin binds to TRESK in vitro, it remains to be established whether the two proteins also interact in the living cell."
24830385
sparser
"The cytoplasmic loop of the distantly related Danio rerio (zebrafish) TRESK (amino acids 174–284) also binds tubulin (as a GST-fusion protein, xref , compare lane 3 to lanes 1 and 2 )."
24830385
sparser
"These observations indicate that the binding of tubulin to TRESK loop does not depend on a single short determinant in the sequence, but there are multiple (at least two) contact points between the two proteins."
24830385
sparser
"In order to further verify and also statistically evaluate the binding of tubulin to the cytoplasmic loop of TRESK, we performed four pairs of independent pull-down assays from mouse brain cytosol with either the bait protein (fragment 174–280) or only the control resin."
24830385
sparser
"In addition to the known TRESK-interacting proteins (calcineurin and 14-3-3), we detected substantial binding of tubulin to the cytoplasmic loop of TRESK."
24830385
sparser
"Together with the mass spectrometry data of the corresponding band from affinity chromatography, we consider it as proof that the cytoplasmic loop of TRESK interacted with tubulin in the pull-down assays."
24830385
reach
"The persistent binding of tubulin to TRESK loop during the long washing step and NaCl gradient encouraged us to also demonstrate the interaction in pull-down assays."
24830385
sparser
"The architectural complexity level of tubulin (e.g. monomer, dimer, oligomer, protofilament, microtubule) interacting with TRESK also has not yet been defined."
24830385
sparser
"The most plausible explanation of this result is that the functional unit of tubulin, the α–β heterodimer associates to TRESK."
24830385
reach
"Our results also hint at the possibility that the interaction between TRESK and tubulin is conditional."
24830385
reach
"P2X2 channel and TRESK may bind tubulin with a similar mechanism."
24830385
sparser
"Therefore we concluded that fragment 256–271 is a major determinant of the interaction of tubulin with the cytoplasmic loop of TRESK."
24830385
sparser
"In fact, the reduced interaction of TRESK loop with tubulin could have been caused by either the binding of 14-3-3 to the loop or the phosphorylation of the 14-3-3-binding site by PKA in this experiment."
24830385
reach
"The above pharmacological and overexpression experiments did not shed light on the functional relevance of the interaction between TRESK and tubulin."
24830385
reach
"These observations indicate that the binding of tubulin to TRESK loop does not depend on a single short determinant in the sequence, but there are multiple (at least two) contact points between the two proteins."
24830385
sparser
"We provided evidence suggesting that the PKA-dependent docking of 14-3-3 interfered with the binding of tubulin to TRESK."
24830385
sparser
"The persistent binding of tubulin to TRESK loop during the long washing step and NaCl gradient encouraged us to also demonstrate the interaction in pull-down assays."
24830385
sparser
"In the following experiments we examined the interaction between TRESK loop and tubulin in a different experimental context."
24830385
sparser
"The above pharmacological and overexpression experiments did not shed light on the functional relevance of the interaction between TRESK and tubulin."
24830385
sparser
"P2X2 channel and TRESK may bind tubulin with a similar mechanism."
24830385