IndraLab

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THR binds USP6. 12 / 12
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"TRE2 in vivo , we carried out ChIP assay on intestinal Analysis of the ChIP DNA by using a primer pair chromatin extracted from premetamorphic tadpoles flanking TRE2 showed that TR was bound to TRE2 in premetamorphic tadpole intestine and T3 treatment enhanced TR binding to TRE2 ( xref )."
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"That is, TR also bound predominantly to the TRE2 in the tail of premetamorphic tadpoles treated with or without T3 for 2 days."
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"TRE2 in vivo, we carried out ChIP assay on intestinal Analysis of the ChIP DNA by using a primer pair chromatin extracted from premetamorphic tadpoles flanking TRE2 showed that TR was bound to TRE2 in premetamorphic tadpole intestine and T3 treatment enhanced TR binding to TRE2 (Figure 5)."
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"More importantly, TRE2 is bound by TR in the tadpole intestine and T3 treatment of premetamorphic tadpoles leads to increased level of the activation histone mark H3K79 methylation and recruitment of RNA polymerase II to the TRE2 in the tadpole intestine."
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"In any case, our findings together indicate that Dot1L gene is a direct T3 target gene that is regulated via binding of the TR predominantly to TRE2."
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"To determine if the strong binding of TRE2 to the TR/RXR heterodimer was sequence-specific, we mutated the putative TRE2 at the positions known to be important for binding to TR/RXR heterodimer (Figure 3B) and determined the effect in the mobility shift assay."
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"On the other hand, as the DNA generated by sonication during the ChIP assay could be as long as 1 kb (typically averaging around 500 bp), we could not rule out the possibility that the signal observed at TRE1 was due to TR binding at TRE2."
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"This could occur if the TRE1 region was present in some large fragments of DNA immunoprecipitated due to TR binding to the TRE2."
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"That is, TR also bound predominantly to the TRE2 in the tail of premetamorphic tadpoles treated with or without T3 for 2days."
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"As indicated above, given the limitation of the ChIP assay, it is possible that the weak TR ChIP signal at TRE1 was a result of TR binding at the TRE2 and TRE2 is the only TRE bound by TR during metamorphosis in the intestine and tail."
22800560
sparser
"More importantly, TRE2 is bound by TR in the tadpole intestine and T3 treatment of premetamorphic tadpoles leads to increased level of the activation histone mark H3K79 methylation and recruitment of RNA polymerase II to the TRE2 in the tadpole intestine."
31585895
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"TR is bound to the Dot1L TRE2 in both intestine and tail of premetamorphic tadpoles."
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