IndraLab

Statements

USP9X activates GPSM1. 6 / 7
| 6
reach
"These data show that ectopic expression of USP9x at a high level increases the staining signal of AGS3, and that this effect of USP9x requires its deubiquitinating activity."
20305814
reach
"The observations that knockdown of USP9x decreases the cellular level of AGS3 (XREF_FIG) and overexpression of USP9x increases the staining intensity of AGS3 (XREF_FIG) imply that USP9x can regulate the level of AGS3."
20305814
reach
"Presumably this indicates that low amounts of USP9x are sufficient to prevent AGS3 degradation."
20305814
reach
"To test whether the ability of USP9x to enhance AGS3 staining depends on its deubiquitinating activity, we repeated the experiment using the UCH domain of USP9x alone, which is the catalytic domain responsible for the deubiquitinase activity of USP9x XREF_BIBR, XREF_BIBR."
20305814
reach
"Relative to the full-length USP9x, which increased the AGS3 staining primarily in cells expressing a high level of transfected USP9x-HA (~ 30% of these cells), the UCH domain led to enhanced AGS3 staining in almost all transfected cells displaying a moderate-to-high level of overexpression (XREF_FIG)."
20305814
reach
"Taken together, our study suggests that USP9x can modulate the level of a subpopulation of AGS3, and this modulation plays a role in regulating the structure of the late Golgi compartments."
20305814