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EGF phosphorylates GSK3B. 38 / 38
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"Its increased expression correlates with prostate cancer progression to castration resistant disease [XREF_BIBR] and it is essential for EGF promoted cell migration and invasion, as well as for EMT markers expression [XREF_BIBR], AKT and GSK3beta phosphorylation induced by EGF, and consequently for beta-catenin stabilization [XREF_BIBR]."
28430640
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"siRNA mediated knockdown of APPL1 in swCLCb H1299 cells reversed the EGF dependent increase in Akt and GSK3beta phosphorylation to levels similar to those in control siRNA treated H1299 cells (XREF_FIG, XREF_SUPPLEMENTARY)."
28171750
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"Since ErbB2-4 receptor phosphorylation is required for substrate interaction and activation through tyrosine phosphorylation [ xref , xref ], a reduction in ErbB receptor activation also resulted in a corresponding decrease in EGF-dependent phosphorylation (activation) of Akt, NFκB, and GSK-3β."
22140606
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"Moreover, expression of NOTCH1-IC remarkably decreased EGF induced stimulation of GSK-3beta phosphorylation by ILK1."
26547035
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"Indeed, EGF significantly increased GSK-3beta phosphorylation between 30-120min (data not shown), with the most pronounced effect at 60min, which was significantly reduced by BUD, but not FP, while total GSK-3beta levels were not affected by BUD or FP."
26739349
sparser
"We next examined the effects of Y27632 on the EGF-induced phosphorylation of Akt and GSK-3β in Panc1 cells, since GSK-3β is a critical downstream element of the PI3K/Akt pathway in EGFR signaling [ xref ]."
21722395
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"Mechanistically, we found that Hsp27 is required for EGF induced AKT and GSK3beta phosphorylation and beta-catenin nuclear translocation."
25130271
trips
"Down-regulation of endogenous XB130 with siRNA reduced c-Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells."
17412687
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"Similarly, we found that EGF and Insulin (GF) treatment of primary MECs and LECs, as well as HME50 cells, increased the phosphorylation of Akt and GSK-3beta (XREF_FIG, XREF_SUPPLEMENTARY))."
21664580
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"The EGF-induced phosphorylation of Akt and GSK-3β were also significantly enhanced when the cells were pretreated with 3 μM Y27632 for 1 h, although the increases were less dramatic than those for either MEK1/2 or p44/p42 MAP kinase."
21722395
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"Lee et al. have reported that the chronic treatment of EGF in cervical cancer cells increased phosphorylation of GSK-3beta, a regulator of Snail [XREF_BIBR], inducing EMT [XREF_BIBR]."
26356073
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"EGF induced the phosphorylation of the phosphatidylinositol 3-kinase (PI3-K)-dependent proteins, Akt and GSK-3beta as well as both p42/44 MAPK and p38 mitogen-activated protein kinases (MAPK)."
18487214
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"For GSK3β, the effects were less clear—for ARPE-19 cells, there was a tendency for less phosphorylation of GSK3β ( xref ), but this was not the case for MDA-MB-231 cells ( xref ); neither cell line demonstrated a significant decrease in EGF-stimulated GSK3β phosphorylation upon LCLAT1 silencing."
39024272
sparser
"siRNA-mediated knockdown of APPL1 in swCLCb H1299 cells reversed the EGF-dependent increase in Akt and GSK3β phosphorylation to levels similar to those in control siRNA-treated H1299 cells ( xref , xref )."
28171750
reach
"Since ErbB2-4 receptor phosphorylation is required for substrate interaction and activation through tyrosine phosphorylation [XREF_BIBR, XREF_BIBR], a reduction in ErbB receptor activation also resulted in a corresponding decrease in EGF dependent phosphorylation (activation) of Akt, NFkappaB, and GSK-3beta."
22140606
sparser
"Mechanistically, we found that Hsp27 is required for EGF-induced AKT and GSK3β phosphorylation and β-catenin nuclear translocation."
25130271
reach
"In RKTG-null mouse embryonic fibroblasts, lypophosphatidic acid (LPA), but not EGF (epidermal growth factor), could stimulate hyperphosphorylation of AKT and GSK3beta, accompanied by increases in phosphorylation of p53 at Ser15 and accumulation of p53, as well as its target genes p21 and p16."
21385899
sparser
"BRAF inhibition blocked EGF-induced phosphorylation of GSK3β in ES2 cells ( Fig. 7 A)."
36828346
sparser
"Aloe-emodin treatment was observed to inhibit EGF-induced phosphorylation of GSK-3β in a dose-dependent manner in a JB6 CI 41-5a cell neoplastic model ( xref )."
34178125
sparser
"The previous researches prompt that HSP27 can regulate EGF-induced AKT and GSK3b phosphorylation, inhibit β-catenin ubiquitinoylation and degradation, and then prevent combination of β-catenin nuclear translocation with Slug promoter, thus promoting EMT to drive tumor development ( xref )."
35116869
sparser
"Subsequently, in the present study, the effects of shikonin on EGF-induced FAK, Akt and GSK3β phosphorylation were explored."
35949620
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"To verify whether Akt pathway was involved in hCSC proliferation, we examined the activation of Akt in hCSCs and found that EGF and bFGF treatment of hCSCs caused a rapid activation of Akt and also au[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17150190
sparser
"As demonstrated in xref , EGF upregulated the phosphorylation of FAK, AKT and GSK3β, while shikonin pre-treatment significantly blocked the EGF-induced FAK, AKT and GSK3β phosphorylation ( xref )."
35949620
sparser
"Down-regulation of endogenous XB130 with siRNA reduced c-Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells."
17412687
sparser
"Its increased expression correlates with prostate cancer progression to castration-resistant disease [ xref ] and it is essential for EGF-promoted cell migration and invasion, as well as for EMT markers expression [ xref ], AKT and GSK3β phosphorylation induced by EGF, and consequently for β-catenin stabilization [ xref ]."
28430640
sparser
"Consistent with the known role of PDK1, IGF-1 and epidermal growth factor (EGF) -induced phosphorylation of AKT and GSK3β was also completely abolished in PDK1 deficient keratinocytes ( xref )."
27184845
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"With kinetic properties that were similar to those of Akt dephosphorylation, CZP substantially dephosphorylated GSK-3β at Ser-9 for up to 4 h ( Fig. 2 A ) and prevented EGF-induced GSK-3β phosphorylat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16542821
reach
"Mechanistically, shikonin blocked the proliferation of cervical cancer cells by downregulating the phosphorylation of FAK, AKT and GSK3beta induced by EGF."
35949620
sparser
"The induction of AKT1 and GSK-3β phosphorylation by EGF can be suppressed by the inhibition of Ca 2+ /CaM signaling [ xref ]."
35163631
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"We further confirmed that EGF-induced GSK3β phosphorylation at Ser9 in RSK2 +/+ MEFs was suppressed in RSK2 −/− MEFs ( Fig. 3 C)."
24055036
sparser
"However, the GSK3β phosphorylation induced by EGF did not totally disappeared in RSK2 −/− MEFs ( Fig. 3 C), indicated that EGF-induced AKT might involve in GSK3β phosphorylation at Ser9."
24055036
sparser
"Indeed, EGF significantly increased GSK-3β phosphorylation between 30–120 min (data not shown), with the most pronounced effect at 60 min, which was significantly reduced by BUD, but not FP, while total GSK-3β levels were not affected by BUD or FP (Fig.  xref )."
26739349
sparser
"While BUD attenuated the EGF-induced phosphorylation of EGFR and its downstream target GSK-3β, FP was not able to do so."
26739349
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"EGF-induced phosphorylation of Akt and GSK-3β, and the subsequent increase in the phosphorylation of the retinoblastoma tumor suppressor protein, as well as an increase in the cyclin D1 protein expres[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20959118
sparser
"Phospho specific western blot analysis indicated that Iturin A caused sharp down regulation of EGF induced phosphorylation of Akt (Ser473 and Thr308) and its downstream proteins GSK3β and FoxO3a in treated groups without altering total proteins in both cell lines ( xref )."
25974307
sparser
"Down-regulation of endogenous XB130 with small interfering RNA (siRNA) reduced c-Src activity, IL-8 production, epidermal growth factor (EGF)-induced phosphorylation of Akt and GSK3β in human lung cancer A549 cells [ xref ]."
21884627
reach
"The previous researches prompt that HSP27 can regulate EGF-induced AKT and GSK3b phosphorylation, inhibit β-catenin ubiquitinoylation and degradation, and then prevent combination of β-catenin nuclear translocation with Slug promoter, thus promoting EMT to drive tumor development (20)."
35116869
sparser
"Moreover, expression of NOTCH1-IC remarkably decreased EGF-induced stimulation of GSK-3beta phosphorylation by ILK1 (B)."
26547035