IndraLab

Statements

PAN2 activates HIF1A. 7 / 7
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"To rule out any effects of USP52 on HIF1A translation, we performed polysome profiling on U2OS cells treated with either NT or USP52 siRNA."
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"Disrupting USP52 containing P-bodies by GW182 depletion reduces HIF1A mRNA."
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"USP52 increases HIF-1alpha protein levels through stabilizing HIF-1alpha mRNA instead of affecting HIF-1alpha ubiquitination [ 69,84 ] ."
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"During a screening of mediators for HIF1A mediated hypoxic response, USP52 and PAN2, a component of P-bodies, was found to promote the stability of HIF1A mRNA in 3 ' UTR dependent manner, as documented with the use of knockdown experiments.156 However, the direct binding of this protein to AREs was not studied, and interactions with de facto RPBs, such as TTP, can possibly happen in P-bodies."
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"To investigate further the role of USP52in the HIF1A mediated hypoxic response, U2OS cells were treated with individual USP52 siRNA duplexes 1 and 3 and exposed to hypoxia."
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"This demonstrates that USP52 knockdown specifically reduces HIF1A mRNA abundance, and supports the previous observation that decreased HIF1A mRNA is associated with up-regulation of HIF2A [XREF_BIBR]."
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"To obtain insight into the observed USP52 mediated stabilization of HIF1A mRNA, we performed proteomic analysis of USP52 immunoprecipitates to identify new USP52 associated proteins."
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