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USP28 binds MYC. 65 / 75
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"XREF_BIBR USP28 was also required for Myc stability, and bound to Myc through an interaction with Fbw7alpha, an F-box protein that was a part of an Skp1-Cullins-F-box (SCF)-type ubiquitin ligase."
30881015
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"Collectively, these data suggest that lactate enhances the USP28-MYC interaction via generation of a local acidic microenvironment ( xref )."
30688660
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"Furthermore, we found that lactate enhanced the interaction of MYC only with USP28 WT ."
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"Past investigations revealed that although many FBW7 domains, including WD40, promoted FBW7’s binding to USP28, the amino terminus of FBW7 contributed to the interaction of FBW7, c-Myc and USP28 [5, 29]."
36879346
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"However, in the absence of FBW7, the direct interaction of USP28 and c-Myc was also observed, during which USP28 was more likely to bind to unphosphorylated peptide [31]."
36879346
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"Although the ubiquitination of c-Myc induced by lanatoside C can be turned over by USP28, what was further demonstrated is that lanatoside C can attenuate the interaction between USP28 and c-Myc."
36879346
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"For example, USP28 binds to Myc and causes the deubiquitination of Myc, resulting in protecting Myc from degradation (XREF_TABLE)."
20531303
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"Previous studies have shown that USP28 does not bind to MYC directly but rather binds MYC through interaction with FBW7 ( xref )."
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"In fact, it was subsequently found that USP28 binds to MYC at the same motif recognized by Fbw7, but only when it is unphosphorylated, and promotes deubiquitination of MYC in the absence of Fbw7.104 Moreover, it has been reported that USP28 dissociates from Fbw7α in response to DNA damage, allowing MYC to be degraded by Fbw7α.105 Adding another layer of complexity in regulating MYC, USP28 was reported to control the stability of Fbw7 by antagonizing its auto-ubiquitination and degradation in specific tissues such as lung, pancreas and liver, leading to destabilization of Fbw7 targets and to dose-dependent effects in Usp28 knockout mice."
31832515
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"Usp28 recruited Fbw7 at all levels but bound Myc only at the highest level of expression."
25437563
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"USP28 does not directly bind to c-Myc but deubiquitinates the c-Myc through interaction with Fbw7 in vivo and in vitro, the E3 ligase of c-Myc [ xref ]."
34201062
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"Moreover, we identified Usp28 as a c-MYC target gene highly expressed in murine and human intestinal cancers, which indicates that USP28 and c-MYC form a positive feedback loop that maintains high c-MYC protein levels in tumors."
24960159
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"Its crystal structure showed that USP28 interacts with MYC through C171 and the MBI domain."
30688660
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"Both the direct and indirect interactions between USP28 and c-Myc contribute to the deubiquitination and stabilization of c-Myc."
36879346
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"Our computational simulation analysis also revealed that USP28 displayed possible binding to Lys 51 and Lys 52 of MYC, which implied that ubiquitination might be involved in USP28’s binding to MYC."
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"The deubiquitinating enzyme USP28 reportedly binds to the N-terminus of FBXW7a for deubiquitination degradation and then induces c-Myc to promote GBM [118–120]."
| PMC
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"The interaction of USP28 with c-Myc was further explored by performing reciprocal IP using an anti-c-Myc antibody, wherein USP28 proteins were positively present in c-Myc immunoprecipitates ( Fig. 5 B[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36442624
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"Among them, USP28 binds to Myc through an interaction with nucleoplasmic FBW7α, which is essential in breast and colon tumor cell proliferation [ xref , xref ], and USP36 binds to nucleolar Fbw7γ and controls the nucleolar degradation pathway of c-Myc in lung and breast cancer cells [ xref , xref ]."
37190313
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"Degradation of c-Myc by Fbw7 can be reversed by the ubiquitin-specific protease, USP28 , which can form a ternary complex with c-Myc and Fbw7α, in the nucleoplasm."
20046830
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"However, in the absence of FBW7, the direct interaction of USP28 and c-Myc was also observed, during which USP28 was more likely to bind to unphosphorylated peptide [ xref ]."
36879346
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"For example, USP28 binds to Myc and causes the deubiquitination of Myc, resulting in protecting Myc from degradation ( xref ) ( xref ; xref )."
20531303
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"USP28 binds to MYC through an interaction with FBW7alpha, an F-box protein that is part of an SCF-type ubiquitin ligase."
17558397
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"Lanatoside C inhibited Wnt and beta-catenin signaling with downregulation of c-Myc, while overexpression of c-Myc reversed the anti-tumor effect of lanatoside C, confirming that c-Myc is a key drug target of lanatoside C. Furthermore, we discovered that lanatoside C prompted c-Myc degradation in proteasome-ubiquitin pathway with attenuating the binding of USP28 to c-Myc."
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"As a ubiquitin specific protease, Usp28 binds to Myc and antagonizes the activity of Fbw7, thereby stabilizing Myc."
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"USP28 forms a ternary complex with αFbxw7/hCdc4 and c-Myc specifically in the nucleus [65] ."
18541364
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"This decline in total protein resulted in decreased endogenous USP28 binding to Fbw7alpha and c-Myc, which increased the levels of Ub-c-Myc."
20046830
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"The CPD motif of c-Myc was essential in mediating the interaction between DUB and substrate, as was Fbw7, which was found to form a complex between c-Myc and USP28 (the ‘piggyback’ model)."
26410034
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"USP28 interacts with MYC and other oncogenic transcription factors, such as Jun and Notch, via the common ubiquitin ligase SCF(FBW7) that recognizes specific phosphodegrons ( xref )."
38227944
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"In fact, it was subsequently found that USP28 binds to MYC at the same motif recognized by Fbw7, but only when it is unphosphorylated, and promotes deubiquitination of MYC in the absence of Fbw7. xref Moreover, it has been reported that USP28 dissociates from Fbw7α in response to DNA damage, allowing MYC to be degraded by Fbw7α. xref Adding another layer of complexity in regulating MYC, USP28 was reported to control the stability of Fbw7 by antagonizing its auto-ubiquitination and degradation in specific tissues such as lung, pancreas and liver, leading to destabilization of Fbw7 targets and to dose-dependent effects in Usp28 knockout mice."
31832515
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"We next investigated the effects of ADI-PEG20 on the interactions between endogenous USP-28 and c-Myc."
22461507
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"USP28 binds to MYC via an interaction with FBW7α ( xref )."
31092557
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"Although the ubiquitination of c-Myc induced by lanatoside C can be turned over by USP28, what was further demonstrated is that lanatoside C can attenuate the interaction between USP28 and c-Myc."
36879346
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"USP28 binds to MYC through an interaction with FBW7alpha, an F-box protein that is part of an SCF-type ubiquitin ligase."
17558397
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"USP28 binds to MYC through an interaction with FBW7alpha, an F-box protein that is part of an SCF-type ubiquitin ligase."
17558397
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"Indeed, USP28 de-ubiquitinates and stabilizes MYC, the USP28/MYC interaction being mediated by the F-box protein, FBW7, which targets MYC for degradation by the proteasome."
17961905
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"It has been reported that deubiquitinase USP28 and c-Myc can form a positive feedback loop that maintains high c-Myc protein levels in tumors xref ."
32350243
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"XREF_FIG shows that ADI-PEG20 treatment enhances the interaction between USP-28 and c-Myc."
22461507
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"USP28 binds to MYC via an interaction with FBW7alpha."
31092557
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"Currently, the mechanism that USP28 interacts with c-Myc and antagonizes c-Myc degradation is controversial."
26771234
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"The effect is limited to FBW7alpha because Usp28 specifically interacts with FBW7alpha and this interaction mediates complex formation between MYC and Usp28."
19489724
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"Several studies suggested that certain types of ubiquitin ligase (Fbw7) are recruited by phosphorylation of c-Myc at Thr58, bridge USP28 and c-Myc interaction, and enhance the ability of USP28 to remove the ubiquitin from c-Myc [ xref , xref ]."
26771234
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"However, our finding shows that down-regulation of USP28 in BR cells attenuates c-Myc stability in a phosphorylation (Thr58/Ser62) site-independent manner, which is in agreement with other studies supporting that USP28 can directly bind to c-Myc in the absence of F-box protein Fbw7 [ xref ]."
26771234
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"In the current study we found that ADI-PEG20 treatment drastically increased the association between USP-28 and c-Myc."
22461507
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"Recent studies showed that USP28 was involved in cancer-related pathways, and regulated physiological homeostasis of ubiquitination process, DNA-damage response, and cell cycle during genotoxic stress, which suggested that USP28 could be a promising target for cancer therapy. xref USP28 required for Myc function was screened. xref USP28 bound to Myc through an interaction with Fbw7α, and catalyzed the deubiquitination of Myc, thereby promoting its stabilization and contributing to tumor cell growth in colon and breast cancers. xref , xref USP28 can also bind to and deubiquitinate some proteins involved in DNA-damage pathways."
30881015
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"Some of these ubiquitination events are opposed by the activity of the deubiquitinase (DUB) USP28; for example, c‐MYC stability in human tumour cells is USP28 dependent and USP28 binds to MYC through a nuclear interaction with FBW7α [ xref ]."
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"Currently, the mechanism that USP28 interacts with c-Myc and antagonizes c-Myc degradation is controversial."
26771234
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"On the other hand, USP28 does not directly interact with Myc, but rather associates with an E3 ligase of Myc, Fbw7, to counteract Fbw7-mediated Myc ubiquitination ( xref )."
24075993
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"We also observed that USP28 could directly interact with and stabilize c-Myc, a transcriptional target of STAT3."
36442624
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"In comparison, the association between c-MYC and USP28 (p = 0.8580; r = 0.001016) and c-MYC and USP36 (p = 0.1139; r = 0.07626) expressions were found statistically insignificant in the ABC subtype of DLBCL (Figure 4A)."
36985413
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"Conversely, the association between c-MYC and USP28 (p = 0.6402; r = 0.004588) and c-MYC and USP37 (p = 0.08387; r = 0.006006) expressions were found to be statistically insignificant in the GCB subtype of DLBCL (Figure 4B)."
36985413
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"Some of these ubiquitination events are opposed by the activity of the deubiquitinase (DUB) USP28; for example, c‐MYC stability in human tumour cells is USP28 dependent and USP28 binds to MYC through a nuclear interaction with FBW7α [22]."
37866880
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"However, our finding shows that down-regulation of USP28 in BR cells attenuates c-Myc stability in a phosphorylation (Thr58 and Ser62) site independent manner, which is in agreement with other studies supporting that USP28 can directly bind to c-Myc in the absence of F-box protein Fbw7 [XREF_BIBR]."
26771234
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"In agreement with the previous study ( xref ), the interaction of c-MYC with USP28 was reduced in the absence of FBW7."
25716680
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"The deubiquitination enzyme USP28 reportedly binds to the N-terminus of FBXW7a for deubiquitinating degradation, and then induces c-Myc to promote the development of GBM [29, 133]."
37060016
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"Lanatoside C treatment decreased the binding of USP28 to c-Myc ( Fig.8 A)."
29475060
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"Emerging evidence demonstrates that USP28 can bind and promote deubiquitination of MYC in the absence of FBW7 in intestinal crypt stem cells."
30688660
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"These data suggest that Usp28 binding to c-Myc can occur via the degron motif in an Fbw7-independent fashion."
25716680
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"Moreover, addition of unphosphorylated degron peptide strongly reduced the interaction between USP28 and c-MYC, presumably by titration of Usp28 protein, while the p-Thr58/p-Ser62 peptide did not affect the interaction ( xref )."
25716680
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"Meanwhile, lanatoside C contributed to c-Myc instability partially via attenuating the interaction between USP28 and c-Myc."
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"Both USP28 and USP36 can bind to c-MYC in cells, and deubiquitinize c-MYC, thereby modulating cancer cell growth and apoptosis (Popov et al., 2007; Sun et al., 2015)."
37251574
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"In addition, lanatoside C, a cardiac glycoside, has been shown to reduce MYC levels and suppress gastric cancer cell proliferation by inhibiting USP28 binding to MYC, thereby destabilizing MYC, although it remains to be determined whether lanatoside C directly targets USP28 ( xref )."
34178666
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"XREF_BIBR USP28 bound to Myc through an interaction with Fbw7alpha, and catalyzed the deubiquitination of Myc, thereby promoting its stabilization and contributing to tumor cell growth in colon and breast cancers."
30881015
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"Together, these findings illustrate that epinephrine stabilizes MYC protein by inducing USP28 and that USP28 Cys 171 directly interacts with the MYC MBI domain ( xref )."
30688660
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"Among them, USP28 binds to Myc through an interaction with nucleoplasmic FBW7α, which is essential in breast and colon tumor cell proliferation [139,140], and USP36 binds to nucleolar Fbw7γ and controls the nucleolar degradation pathway of c-Myc in lung and breast cancer cells [141,142]."
37190313
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"The anti-cancer activities of lanatoside C were possibly mediated by the inhibition of Wnt signaling pathway with c-Myc ubiquitination via attenuating the interaction between c-Myc and USP28 ( Fig.9 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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