IndraLab

Statements

CAV3 binds KCNMA1. 16 / 17
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sparser
"A potential interaction between Cav3 and KCa1.1 channels is supported by a report of coimmunoprecipitation between Cav3.2 and KCa1.1 proteins from mouse brain lysates xref , and at least a functional coupling between Ni 2+ -sensitive calcium influx and KCa1.1-mediated AHPs in neurons of the medial vestibular nucleus (MVN) xref ."
23626738
sparser
"The Cav3-KCa1.1 interaction could be reproduced in tsA-201 cells upon coexpressing only the α-subunits of Cav3.2 and KCa1.1, indicating that these are sufficient to account for the principal interaction between the two channels."
23626738
biogrid
No evidence text available
15703204
sparser
"However, a key difference between the Cav3-KCa1.1 interaction recorded in MVN and tsA-201 cells was the degree of KCa1.1 activation by a single command pulse."
23626738
biogrid
No evidence text available
15703204
sparser
"To test this we constructed a model of the KCa1.1-Cav3 interaction and found that multiple Cav3 channels are required to provide reliable activation of KCa1.1 channels, perhaps acting cooperatively over larger distances."
23928916
sparser
"Interaction between Cav3 and KCa1.1 Channels."
23626738
sparser
"The Cav3-KCa1.1 interaction was found to function highly effectively in a subset of MVN neurons by activating near –50 mV to contribute to spike repolarization and gain of firing."
23626738
reach
"A computational model of the KCa1.1 and Cav3 complex suggested that multiple Cav3 channels were necessary to activate KCa1.1 channels, potentially causing the KCa1.1 and Cav3 complex to be more susceptible to calcium chelators."
23928916
sparser
"However, the greatest influence of the Cav3-KCa1.1 interaction in MVN cells was detected under current-clamp conditions as a (cell-specific) change in repolarization or gain of firing frequency, revealing activation of Cav3 current during the spike response."
23626738
sparser
"However, direct biophysical tests for a Cav3-KCa1.1 interaction have not been reported, and the molecular sites that would support an association between Cav3 and KCa1.1 channels have not been determined."
23626738
sparser
"Finally, applying mibefradil to MVN cells under current clamp slowed spike repolarization, reduced a subsequent fAHP (Fig.  xref ), and increased the gain of firing, revealing that the Cav3-KCa1.1 interaction normally reduces spike output in MVN cells."
24413887
reach
"To determine if Cav3.2 channels associate with KCa1.1 at the molecular level we coexpressed the alpha-subunits of Cav3.2 and KCa1.1 in tsA-201 cells, and were able to coimmunoprecipitate the proteins (XREF_FIG)."
23626738
sparser
"To examine the potential interaction between Cav3 and KCa1.1 channels, we transiently expressed cDNA for both channels in tsA-201 cells."
23626738
reach
"Interaction between Cav3 and KCa1.1 Channels."
23626738
sparser
"These results strongly suggest that cav3 molecule interacts with KCa1.1 at the same site as cav1 does."
23237801