IndraLab

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USP37 deubiquitinates WAPL. 3 / 3
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"Importantly, when USP37 WT, but not USP37 C350A, is added, ubiquitylation is reduced, indicating that USP37 can directly deubiquitylate WAPL and/or WAPL associated proteins in vitro."
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"Next, to investigate whether USP37 can directly deubiquitylate WAPL, we purified full-length USP37 WT and USP37 C350A from Sf9 insect cells and added either USP37 WT, USP37 C350A, or buffer alone to a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"WAPL is pulled down with His Ub under denaturing conditions in all tested treatments, indicating that ubiquitylated WAPL is detected throughout the cell cycle.We expected that, if USP37 deubiquitylate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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