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USP25 inhibits SGCG. 6 / 6
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"The input tagged proteins were verified with the indicated antibodies.doi: 10.1371/journal.pone.0080976.g002 USP25 Negatively Regulates Type I IFN Signaling PLOS ONE | www.plosone.orgdoi: 10.1371/journal.pone.0080976.g003 USP25 Negatively Regulates Type I IFN Signaling PLOS ONE | www.plosone.org manner."
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"However, the catalytic mutants (C178A and H607A) devoid of DUB activity lost the ability of USP25 WT-mediated IFN inhibition to some degree, indicating that DUB activity is involved in USP25 inhibition of type I IFN induction.To further determine the levels at which USP25 negatively regulates type I IFN signaling, HEK-293T cells were transfected with DNA constructs encoding RIG-I, IPS-1, TRAF2, or TRAF6, together with IFN-β-Luc."
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"However, the catalytic mutants (C178A and H607A) devoid of DUB activity lost the ability of USP25 WT-mediated IFN inhibition to some degree, indicating that DUB activity is involved in USP25 inhibition of type I IFN induction.Sequence for siRNAs of human USP25."
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"*P < 0.05 for all pairwise comparisons by one-way ANOVA followed by Dunnett's multiple comparisons test.doi: 10.1371/journal.pone.0080976.g006 USP25 Negatively Regulates Type I IFN Signaling PLOS ONE | www.plosone.orgUSP25 deubiquitinates RIG-I, TRAF2 and TRAF6."
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"To examine whether the inhibitory effects of USP25 on SEV-induced type I IFN signaling is due to its deubiquitinase activity, wild-type USP25 (USP25-WT) and its mutants (C178A and H607A) lacking DUB activity were co-transfected with the promoter luciferase reporter plasmid of IFN-β, IRF3, NF-κB and ISRE, and the luciferase activity was detected."
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"doi: 10.1371/journal.pone.0080976.g005 USP25 Negatively Regulates Type I IFN Signaling PLOS ONE | www.plosone.org 7B), TRAF2 (Figure 7D), and TRAF6 (The deubiquitinating activity of USP25 is involved in virus-induced type I IFN signaling."
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