IndraLab
Statements
reach
"XREF_BIBR found that alcohol causes remodeling of the BK channel transcript landscape by regulating the stability of a subset of these transcripts, such that the relative number of transcripts encoding highly sensitive channels are decreased, whereas that of transcripts encoding less sensitive channel proteins are increased, consistent with tolerance."
reach
"The ethanol " opposing " effects on macroscopic current (XREF_FIG) were matched by the results describing drug behavior at single-channel resolution, with the alcohol increasing BK channel steady-state activity (NPo) at 0.3 muM Ca 2+ i while inhibiting activity at 30 muM Ca 2+ i (XREF_SUPPLEMENTARY)."
reach
"The mammalian BK channel is also activated by ethanol, and this effect, as well as the channel 's basal activity, depends on the thickness of the lipid bilayer; channels that reside in thicker membrane are less basally active and are less activated by ethanol XREF_BIBR, XREF_BIBR, suggesting that movement of the BK channel into and out of areas of thicker membrane microenvironments may allow for very fast modulation of the effects of ethanol on this channel XREF_BIBR."
sparser
"The mammalian BK channel is also activated by ethanol, and this effect, as well as the channel’s basal activity, depends on the thickness of the lipid bilayer; channels that reside in thicker membrane are less basally active and are less activated by ethanol xref , xref , suggesting that movement of the BK channel into and out of areas of thicker membrane microenvironments may allow for very fast modulation of the effects of ethanol on this channel xref ."
reach
"For example, expression of brain derived neurotrophic factor (Bdnf) and synaptophysin (Syp) were decreased while Kcnma1, Arl6ip5 and Camkk2 were increased by acute ethanol in PFC of Fyn null animals (XREF_SUPPLEMENTARY, lower panel) but were not regulated by ethanol in controls (XREF_SUPPLEMENTARY, upper panel)."
reach
"On the other hand, ethanol directly modulates BK channel activity in a variety of systems, including rat neurohypophysial nerve endings, primary sensory dorsal root ganglia, nucleus accumbens neurons, Caenorhabditis elegans type-IV dopaminergic CEP neurons, and nonneuronal preparations, such as rat pituitary cells, cerebrovascular myocytes and human umbilical vein endothelial cells."
reach
"Filling this knowledge gap acquires particular relevance because ethanol action on BK channels appears to be beta subunit dependent; at physiological Ca 2+ i levels, neuronal and recombinant (slo1 and slo1+ beta4) channels are usually activated by ethanol, whereas vascular smooth muscle and recombinant slo1+ beta1 are usually inhibited."