IndraLab

Statements

PAN2 binds MYC. 7 / 7
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"T-REx-HeLa cells were co-transfected with pCMV-2 × TO-5 × Myc-Pan2 and a plasmid expressing 5 × Flag-β-globin reporter mRNA."
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"The expression of 5 × Myc-Pan2, PAT shortening of the reporter mRNA and SG formation were monitored ( xref )."
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"Time-dependent induction of 5 × Myc-Pan2 and shortening of the reporter mRNA PAT were observed after tetracycline treatment ( xref )."
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"T-REx HeLa cells were co-transfected with a plasmid expressing c-myc mRNA, a reference plasmid expressing 5 × Flag-GST-CAT, and either pCMV-5 × Myc-Pan2 D1083A, pCMV-5 × Myc-Caf1 D161A or, as a contro[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25446091
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"We expressed 5 × Myc-Pan2, 5 × Myc-Pan2 D1083A mutant, 5 × Myc-Caf1, or 5 × Myc-Caf1 D161 mutant and analyzed PB formation by indirect immunofluorescence using the PB marker protein DDX6."
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"Then, we individually mutated these lysine (K) residues to arginine (R) residues and coexpressed them with Myc‐USP52."
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"HeLa cells were co-transfected with plasmids expressing Flag-β-globin mRNA and either 5 × Myc-Pan2 or 5 × Myc-Pan2 D1083A, which has no deadenylase activity and acts as dominant-negative to the Pan2 deadenylase activity ( xref ), and the mRNA was analyzed by northern blotting."
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