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OTUD6A binds CDC6. 12 / 12
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"We further investigated the role of interaction between CDC6 and OTUD6A in CDC6 protein regulation."
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"Although mutation of OTUD6A did not affect the interaction between OTUD6A and CDC6 (Supplementary Fig.  xref h), the C152A mutant of OTUD6A lost the ability to upregulate CDC6 (Fig.  xref h, i and Supplementary Fig.  xref i-m)."
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"OTUD6A directly interacts with CDC6."
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"Through a screening of DUBs, we found that OTUD6A interacts with CDC6 and increases the CDC6 protein level by promoting the stability of CDC6 through removing polyubiquitin chains, whose attachment was mediated by SCF-Cyclin F and APC/C-CDH1 (Fig.  xref )."
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"However, overexpression of OTUD6A-C-terminus, which could not interact with CDC6, lost its ability to promote cell proliferation (Supplementary Fig.  xref f-h)."
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"However, the interaction between CDC6 and OTUD6A was not changed in cells treated with the CDK2 inhibitor CVT-313 compared with that in control cells (Supplementary Fig.  xref j)."
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"We showed that DNA damage promotes the binding of OTUD6A to CDC6, subsequently upregulating CDC6 protein level, activating the ATR-Chk1 pathway and resulting in chemoresistance (Fig.  xref )."
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"We next examined whether OTUD6A physically interacts with CDC6."
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"Bimolecular fluorescent complimentary (BiFC) assays confirmed that OTUD6A directly interacts with CDC6 (Fig.  xref f)."
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"GST pulldown and assays confirmed the direct interaction between the N-terminal region of OTUD6A and CDC6 (Fig.  xref g)."
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"Importantly, the interaction between OTUD6A and CDC6 was detected in late S phase and occurred predominantly in G2/M phase, following the same trend as CDC6 protein level (Fig.  xref h, i)."
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"HU treatment, which increased CDC6 expression, effectively promoted the interaction between OTUD6A and CDC6, indicating that replication stress enhanced the binding capacity between OTUD6A and CDC6 (Fig.  xref j)."
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