IndraLab

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"The SLO treatment markedly increased the intensity of PI staining in the cells, while a gradual decrease in PI staining was observed in cells after SLO removal and recuperation in new culture medium over time, and 30 min of recuperation resulted in undetectable levels of intracellular PI (Fig. 3g, h) and viable cells (Supplementary Figure 3c)."
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"Primary mouse flexor digitorum brevis muscle fibers were also examined after exposure to SLO or SM after 30 s. Without Ca 2+, SLO triggered PI influx and staining of the fiber nuclei."
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"Results obtained showed that SLO induced cell permeabilization (100% PI positive cells), whereas the presence of HSA in the medium completely inhibited the SLO dependent permeabilization (XREF_FIG)."
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"In non muscle cells, including mouse embryonic fibroblasts (MEFs) and bone marrow derived macrophages (BMMs), SLO treatment caused more PI positive cells for ML1 KO (XREF_FIG & XREF_SUPPLEMENTARY)."
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