IndraLab
Statements
reach
"Although after 100 d of chase (+ doxycycline) 11.0 +/- 2.3% of CYLDex7/8 WT SKLCD150 + CD48 - CD34 - cells retained the H2B-GFP label, only 5.6 +/- 1.02% of mutant cells remained GFP positive (XREF_FIG), demonstrating that CYLD binding to TRAF2 is a crucial step to maintain HSC dormancy."
sparser
"Our study adds a novel level of complexity to this scenario, revealing that the interaction between the DUB CYLD and the adaptor protein, E3 ubiquitin ligase TRAF2, elicits a signaling pathway that promotes dormancy and thus prevents HSCs from unscheduled proliferation and exhaustion."
sparser
"Although after 100 d of chase (+doxycycline) 11.0 ± 2.3% of CYLDex7/8 WT SKLCD150 + CD48 − CD34 − cells retained the H2B-GFP label, only 5.6 ± 1.02% of mutant cells remained GFP positive ( xref ), demonstrating that CYLD binding to TRAF2 is a crucial step to maintain HSC dormancy."
sparser
"The biochemical analysis using phosphomimetic mutants demonstrated that this PTM negatively affects the deubiquitinating activity of CYLD on TRAF2, most likely through interfering with the catalytic activity of CYLD, since the binding of TRAF2 to a CYLD mutant mimicking phosphorylation on Ser 418 is not affected (Fig. xref a; [ xref ])."