IndraLab

Statements

MTOR phosphorylates EIF4EBP1 on T46. 31 / 31
1 2 6 1 | 11 8 2
rlimsp
"Taken together, our results suggest that 4E-BP1 phosphorylation by FRAP/mTOR on Thr-37 and Thr-46 is a priming event for subsequent phosphorylation of the carboxy-terminal serum-sensitive sites."
10364159
reach
"When 4E-BP1 is phosphorylated by mTOR, first at critical priming threonine (T) 37 and T46 residues and then at other sites, 4E-BP1 is inactivated and releases eIF4E to allow initiation of cap dependent translation."
25883264
sparser
"Immunoprecipitated mTOR phosphorylates 4E-BP1 on Thr37 and Thr46 in vitro , indicating that mTOR can directly phosphorylate these two sites [ xref , xref , xref ]."
21190521
hprd
No evidence text available
18669648
reach
"MTOR first phosphorylates Thr37 and Thr46 on 4E-BP1 (eIF-4E-binding protein 1) complexed with eIF4E (eukaryotic initiation factor)."
35834616
phosphoelm
No evidence text available
12370290
sparser
"Over expression of eIF4E can lead to increased cell proliferation, transformation and tumorigenesis. xref 4E-BP1, known as PHAS-1, normally binds eIF4E, inhibiting cap-dependent translation and is phosphorylated in vivo on multiple residues; phosphorylation by mTOR on Thr37 and Thr46 of human 4E-BP1 may be very important for subsequent phosphorylation. xref EIF4E protein levels were unchanged with the progression of cell series ( xref )."
19291795
reach
"Gels were transferred to nitrocellulose membranes and incubated with antibodies, diluted 1:1000 unless otherwise stated, directed to phosphorylated Ser2448 of mTOR, LAMP1, 4E-BP1, phosphorylated Thr 37 and Thr 46 of 4E-BP1, GAPDH, p70S6K, LAMP2 diluted 1:100 and beta-actin diluted 1:2000."
26572924
reach
"Mechanistically, phosphorylation of 4E-BP1 at Thr 37/46 by the mammalian target of rapamycin (mTOR) induces its dissociation from the eukaryotic translation initiation factor 4E (eIF4E) providing the assembly of the preinitiation complex."
23769948
reach
"4E-BP1 is phosphorylated by mTOR at T37 and T46."
24303063
hprd
No evidence text available
19651622
signor
"These results indicate that arg, leu, and gln act coordinately to stimulate proliferation of ptr cells through activation of the mtor-rps6k-rps6-eif4ebp1 signal transduction pathway. Specifically as part of mtorc1, mtor directly phosphorylates the ribosomal protein s6 kinases (s6k1 and s6k2) and the eukaryotic initiation factor 4e (eif4e)-binding proteins (4e-bp1 and 4e-bp2), both of which control specific steps in the initiation of cap-dependent translation"
23486913
rlimsp
"The phosphorylation of 4E-BP1 at Thr 46 (a site phosphorylated by mTOR) was monitored with a phospho-specific antibody during a time course following 10 Gy of IR."
15070431
biopax:phosphositeplus
No evidence text available
sparser
"The 4EBP1 protein is directly phosphorylated by mTOR on T37 and T46, which primes subsequent phosphorylation on S65 and T70 ( xref , xref )."
20227039
hprd
No evidence text available
19664995
reach
"The 4EBP1 protein is directly phosphorylated by mTOR on T37 and T46, which primes subsequent phosphorylation on S65 and T70."
20227039
hprd
No evidence text available
11777913
sparser
"MTOR first phosphorylates Thr37 and Thr46 on 4E-BP1 (eIF-4E-binding protein 1) complexed with eIF4E (eukaryotic initiation factor)."
35834616
hprd
No evidence text available
20068231
sparser
"MTOR-dependent phosphorylation of 4E-BP1 at Thr37 and Thr46 primes 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 and, therefore, is an indicator of 4E-BP1 activity ( xref )."
23972597
reach
"Taken together, our results suggest that 4E-BP1 phosphorylation by FRAP and mTOR on Thr 37 and Thr 46 is a priming event for subsequent phosphorylation of the carboxy-terminal serum sensitive sites."
10364159
reach
"The mTOR dependent phosphorylation of 4EBP1 on Thr 37 and Thr 46 serves as a priming event that allows a further phosphorylation of Thr 70, Ser 65, and possibly other sites by additional signaling med[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16242075
reach
"The mTOR kinase complex 1 (mTORC1), a downstream target of both AKT and ERK signaling [XREF_BIBR, XREF_BIBR], is a master regulator of eIF4E initiated cap dependent translation by phosphorylating 4E-BP1 on Thr37 and Thr46 that act as priming sites for its subsequent phosphorylation on Ser65 and Thr70 [XREF_BIBR]."
24970798
sparser
"4E-BP1 is phosphorylated by mTOR at T37 and T46."
24303063
signor
"Here, we show that a functional TOS motif is required for 4E-BP1 to bind to raptor (a recently identified mTOR-interacting protein), for 4E-BP1 to be efficiently phosphorylated in vitro by themTOR/raptor complex, and for 4E-BP1 to be phosphorylated in vivo at all identified mTOR-regulated sites. mTOR/raptor regulated phosphorylation is necessary for 4E-BP’s efficient release from the translational initiation factor eIF4E. We find that the TOS motif is absolutely required for efficient phosphorylation of 4E-BP1 at all the identified mTOR-regulated sites, namely, Thr37/46, Ser65, and Thr70 in vivo."
12747827
hprd
No evidence text available
19413330
reach
"Mechanistically, phosphorylation of 4E-BP1 at Thr-37/46 by the mammalian target of rapamycin (mTOR) induces its dissociation from the eukaryotic translation initiation factor 4E (eIF4E) providing the assembly of the preinitiation complex (Youtani et al., 2000)."
23769948
sparser
"MTOR-dependent phosphorylation of serine Ser 65 and N-terminal Thr 37 , Thr 46 , and Thr 70 in 4E-BP1 cleaves 4E-BP1 from the translational activator eIF-4E ( xref )."
17717968
reach
"MTOR dependent phosphorylation of 4E-BP1 at Thr37 and Thr46 primes 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 and, therefore, is an indicator of 4E-BP1 activity."
23972597
sparser
"The mTOR-dependent phosphorylation of 4EBP1 on Thr-37 and Thr-46 serves as a priming event that allows a further phosphorylation of Thr-70, Ser-65, and possibly other sites by additional signaling med[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16242075