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AMPK phosphorylates USP10. 25 / 25
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"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 +/-0.04 mol and mol, which was also higher than that of SAMS (0.35 +/-0.02 mol and mol) (XREF_FIG)."
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"We first tested whether USP10 could be phosphorylated by AMPK using a pan-AMPK substrate antibody."
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"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay."
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"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 +/- 0.04 mol and mol, which was also higher than that of SAMS (0.35 +/- 0.02 mol and mol)."
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"We found that AMPK specifically phosphorylated USP10 but not GST in vitro ( Figure 4 C), suggesting that USP10 is an AMPK substrate."
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"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46 ± 0.04 mol/mol, which was also higher than that of SAMS (0.35 ± 0.02 mol/mol) ( Figure 4 E)."
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"Phosphorylation of USP10 by AMPK increases its activity."
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"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished ( xref )."
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"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished (XREF_FIG)."
26876938
sparser
"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26876938
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"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay (D[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished ( Figure 4 F)."
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"AMPK phosphorylates USP10 under energy stress."
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"AMPK phosphorylates USP10 under energy stress."
26876938
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"We first tested whether USP10 could be phosphorylated by AMPK using a pan-AMPK substrate antibody."
26876938
reach
"We therefore tested whether USP10 could be phosphorylated by AMPK and whether this phosphorylation would affect USP10 activity."
26876938
reach
"We found that AMPK specifically phosphorylated USP10 but not GST in vitro, suggesting that USP10 is an AMPK substrate."
26876938
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"On the other hand, USP10 is phosphorylated and activated by AMPK under energy stress."
26876938
reach
"After the S76 is mutated to Ala, the phosphorylation of USP10 by AMPK in vitro was totally abolished."
26876938
sparser
"We therefore tested whether USP10 could be phosphorylated by AMPK and whether this phosphorylation would affect USP10 activity."
26876938
sparser
"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay ( xref )."
26876938
reach
"Phosphorylation of USP10 by AMPK increases its activity."
26876938
reach
"We found that AMPK specifically phosphorylated USP10 but not GST in vitro (XREF_FIG), suggesting that USP10 is an AMPK substrate."
26876938
sparser
"We found that AMPK specifically phosphorylated USP10 but not GST in vitro ( xref ), suggesting that USP10 is an AMPK substrate."
26876938
sparser
"The maximal stoichiometry of phosphorylation of USP10 peptide by AMPK was 0.46±0.04 mol/mol, which was also higher than that of SAMS (0.35±0.02 mol/mol) ( xref )."
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