IndraLab

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USP8 binds RNF41. 34 / 36
5 1 | 9 19
sparser
"Lastly, to ambiguously demonstrate the interactions between Nrdp1 and USP8 and between USP8 and HIF-1α in OGD-treated neurons, we performed co-immunoprecipitation experiments."
28979191
sparser
"Nrdp1 and USP8 could be coimmunoprecipitated, and in transfected cells USP8 specifically bound to Nrdp1 but not cbl, a RING finger E3 ligase involved in ligand-stimulated epidermal growth factor receptor down-regulation."
15314180
sparser
"As expected, Clec16a- specific shRNA reduced Nrdp1-USP8 binding in situ ( xref )."
29180353
sparser
"To assess the role of Clec16a-mediated ubiquitination on formation of the β-cell mitophagy complex, we utilized a proximity ligation assay (PLA) to visualize Nrdp1-USP8 association in situ after lenalidomide treatment ( xref )."
29180353
reach
"While Clec16a enhanced Nrdp1-USP8 complex assembly, addition of the ubiquitin KO mutant reduced Nrdp1 levels as well as Nrdp1 interaction with both Clec16a and USP8 (Fig. 4E)."
29180353
reach
"USP8 interactions with Nrdp1 and BRUCE."
31845722
sparser
"Indeed, we found that the interaction of NRDP1 and USP8 was decreased following a 48 h exposure to palmitate and high glucose in both beta cell lines as well as primary human beta cells by PLA ( xref and reference xref )."
31107439
sparser
"While Clec16a enhanced Nrdp1-USP8 complex assembly, addition of the ubiquitin KO mutant reduced Nrdp1 levels as well as Nrdp1 interaction with both Clec16a and USP8 ( xref )."
29180353
biogrid
No evidence text available
17035239
sparser
"Also, RNF41 can interact with the deubiquitylating enzyme USP8 (also called UBPy), which is required for maintenance of ESCRT-0 stability and endosomal sorting of ErbB3 and EGFR ( xref ; xref )."
21378310
sparser
"Nrdp1-USP8 binding was also decreased after palmitate treatment, indicating that glucolipotoxicity destabilizes upstream mitophagy regulators ( xref )."
29180353
biogrid
No evidence text available
17035239
sparser
"Similarly, glucolipotoxicity decreased Nrdp1-USP8 interaction in primary human β-cells ( xref )."
29180353
biogrid
No evidence text available
15314180
reach
"Nrdp1 interacts with two upstream regulators of mitophagy: Clec16a and the deubiquitinase (DUB) enzyme USP8 (5,25)."
29180353
reach
"Also, RNF41 can interact with the deubiquitylating enzyme USP8 (also called UBPy), which is required for maintenance of ESCRT-0 stability and endosomal sorting of ErbB3 and EGFR."
21378310
sparser
"Specifically, MAGEL2 interacts with TRIM27 and USP7 to modify the ubiquitination and stability of WASH1 [ xref ], and interacts with RNF41 and USP8 to modify the ubiquitination and stability of the ESCRT-0 (Endosomal Sorting Complexes Required for Transport) complex [ xref ]."
32315313
sparser
"Lenalidomide impaired Nrdp1-USP8 interaction in Min6 β-cells ( xref ), suggesting that Clec16a-mediated ubiquitination maintains the Clec16a-Nrdp1-USP8 complex as well as β-cell function."
29180353
sparser
"The biological significance of the USP8-Nrdp1 interaction is at least partially due to its effect on EGFR regulation."
18687060
biogrid
No evidence text available
15314180
reach
"First, Nrdp1 overexpression leads to increased protein ubiquitylation and suppressed interaction between Nrdp1 and USP8 (due to increased USP8 degradation)."
28979191
biogrid
No evidence text available
28973533
sparser
"There was evidence that Magel2 was involved in the leptin-POMC of the hypothalamus to maintain the energy balance of the body.[ xref ]Wijesuriya et al have found that MAGEL2 and Necdin connected leptin receptor (LepR) to the USP8-RNF41 ubiquitin complex through the LepR adapter protein, IRS4."
34128869
hprd
No evidence text available
15314180
sparser
"The crystal structure of the rhodanese domain of USP8 in complex with Nrdp1 was solved and revealed that the USP8-Nrdp1 interaction appeared to be dominated by salt bridges [ xref ]."
18687060
sparser
"However, palmitate treatment did not additively reduce Nrdp1-USP8 binding in shClec16a-treated Min6 β-cells ( xref ), suggesting that palmitate acts to destabilize the Nrdp1-USP8 complex through effects on the Clec16a pathway."
29180353
reach
"Nrdp1 and USP8 could be coimmunoprecipitated, and in transfected cells USP8 specifically bound to Nrdp1 but not cbl, a RING finger E3 ligase involved in ligand stimulated epidermal growth factor receptor down-regulation."
15314180
reach
"Nrdp1 binds and opposes USP8 action on cytokine receptor signaling (42), and Clec16a could function through Nrdp1 to counter USP8 effects on Parkin."
29180353
sparser
"Importantly, Clec16a overexpression in palmitate-treated cells raised Nrdp1 levels, Nrdp1 ubiquitination, and Nrdp1-USP8 binding back to baseline levels (BSA alone) ( xref )."
29180353
sparser
"We next determined the role of ubiquitination and Clec16a in stabilization of Nrdp1-USP8 binding utilizing WT and mutant ubiquitin."
29180353
sparser
"Utilizing the sensitive proximity ligation assay technique to detect interaction of the mitophagy regulators NRDP1 and USP8, we are able to specifically quantify formation of essential mitophagy complexes in situ."
31107439
reach
"However, palmitate treatment did not additively reduce Nrdp1-USP8 binding in shClec16a-treated Min6 β-cells (Fig. 6A), suggesting that palmitate acts to destabilize the Nrdp1-USP8 complex through effects on the Clec16a pathway."
29180353
reach
"Utilizing the sensitive proximity ligation assay technique to detect interaction of the mitophagy regulators NRDP1 and USP8, we are able to specifically quantify formation of essential mitophagy complexes in situ."
31107439
sparser
"To determine whether the combination of reduced Clec16a expression and/or glucolipotoxicity impairs the upstream mitophagy complex, we assessed Nrdp1-USP8 interaction by PLA."
29180353