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PKA phosphorylates CACNA1C. 26 / 27
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sparser
"Multiple mechanisms may contribute to the impaired β-adrenergic signaling in human HFrEF including altered PKA-dependent phosphorylation of Cav1.2 (and other Ca 2+ -regulating proteins)."
39769475
sparser
"The results of this study convincingly show that isoprenaline-induced, PKA-dependent phosphorylation of Cav1.2 in LV myocardium fromCacna1c +/− rats is attenuated compared with WT, thus explaining the attenuated increases in sarcolemmal Ca 2+ influx."
39769475
reach
"PKA and CaMKII phosphorylate Cav1.2, RyR2, and PLB (among other proteins) to mediate the positive-inotropic and -lusitropic effects of sympathetic stimulation [4]."
37372947
rlimsp
"This mutation does not alter basal Ca(V)1.2 current properties or regulation of Ca(V)1.2 current by PKA and the beta-adrenergic receptor, but abolishes Ca(V)1.2 phosphorylation by PKA."
22216029
sparser
"By contrast, inCacna1c +/− myocytes, in the absence of β-adrenergic stimulation, there is greatly increased phosphorylation of both Cav1.2 and RyR2 at PKA sites S1928 (+110%) and S2808 (+120%), respectively, as depicted by some phosphorylated (P) residues (yellow circles)."
39769475
rlimsp
"We propose that direct phosphorylation of the Cav1.2 by cAMP-dependent protein kinase is sufficient to alter the conformation of the channel and increase calcium influx during sympathetic stimulation."
29123182
sparser
"Activation of β-adrenergic receptors is known to lead to an increase of both L-Type Ca 2+ current (I CaL ) and slow delayed rectifier I Ks , via protein kinase A (PKA)-mediated phosphorylation of Cav1.2 and Kv7.1 channels, respectively."
35919344
sparser
"The α1C subunit is phosphorylated by PKA in intact hippocampal neurons, and a two-fold increase in Ca 2+ influx has been observed in hippocampal neurons in old rats compared with adult rats, suggestive of increased PKA phosphorylation of Cav1.2 with aging."
35111050
sparser
"Maximal PKA phosphorylation of Cav1.2 and RyR2 is reduced inCacna1c +/− compared to WT myocytes, leaving “empty”, i.e., non-phosphorylated serine residues (S1928 and S2808) in Cav1.2 and RyR2 (see white circles) and suggesting—once again—altered local regulation of cAMP and PKA-dependent phosphorylation in the dyadic cleft ofCacna1c +/− myocytes."
39769475
rlimsp
"PKA phosphorylation of the full length Cav1.2 (long-NT) isoform alters function."
29123182
sparser
"PKA and CaMKII phosphorylate Cav1.2, RyR2, and PLB (among other proteins) to mediate the positive-inotropic and -lusitropic effects of sympathetic stimulation [ xref ]."
37372947
sparser
"Some of the cardinal debated issues include details of specific subunits and residues in CaV1.2 phosphorylated by PKA, the nature, extent, and role of post-translational processing of CaV1.2, and the role of auxiliary proteins (such as A kinase anchoring proteins) involved in PKA regulation."
23948586
reach
"In fact, perhaps the best known effect of β-AR stimulation in the heart is the activation of the cascade of Gαs protein and adenylyl cyclase that leads to the phosphorylation of the Cav1.2 channel by [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23811359
sparser
"Catecholamines evoked increased cAMP responses, in turn activating phosphorylation of downstream targets RyR2, PLN, TnI, and Cav1.2 by PKA, increasing beating frequency and calcium kinetics [222] ."
34990783
reach
"Although the two phenomena occur simultaneously during physiological exercise, we separated them to dissect their relative contributions.Activation of β-adrenergic receptors is known to lead to an increase of both L-Type Ca current (I ) and slow delayed rectifier I , via protein kinase A (PKA)-mediated phosphorylation of Cav1.2 and Kv7.1 channels, respectively."
35919344
sparser
"In conclusion, our study reveals altered PKA-dependent phosphorylation of Cav1.2 with elevated phosphorylation of serine-1928 under basal conditions and a diminished phosphorylation reserve during β-adrenergic stimulation."
39769475
sparser
"Although phosphorylation of Cav1.2-pS1928 is not required for β-adrenoceptor stimulation of LTCC in mouse ventricular myocytes and HEK293 cells, xref it is a well-established Cav1.2 PKA phosphorylation site xref and phosphorylation of its α 1C subunit is relevant for channel function by increasing trafficking to the plasma membrane. xref In CON, the relative Cav1.2-pS1928 phosphorylation was significantly lower in cAF compared to SR and increased significantly after incubation with ISO or PF to levels seen in corresponding CONs ( xref and xref , xref ), pointing to significant contribution of PDE8 to LTCC dephosphorylation."
36810794
reach
"In conclusion, our study reveals altered PKA-dependent phosphorylation of Cav1.2 with elevated phosphorylation of serine-1928 under basal conditions and a diminished phosphorylation reserve during β-adrenergic stimulation."
39769475
sparser
"Moreover, phosphospecific antibodies targeted against this site are available, enabling direct monitoring of the PKA-dependent phosphorylation of Cav1.2."
39769475
sparser
"Here, we used such a phosphospecific antibody against S1928 in order to characterize the PKA-dependent phosphorylation of Cav1.2 and its potential role in the impaired sympathetic stress response ofCacna1c +/− rats."
39769475
reach
"Below, we will focus on two areas of calcium channel phosphorylation where recent progress stands out in our opinion — PKA dependent phosphorylation of L-type calcium channels and the identification o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27768908
rlimsp
"One sample of each pair but not the other was phosphorylated in vitro with purified PKA catalytic subunit under conditions that lead to near complete phosphorylation of the PKA site on CaV1.2, as quantified earlier (36)."
29180451
rlimsp
"Some of the cardinal debated issues include details of specific subunits and residues in CaV1.2 phosphorylated by PKA, the nature, extent, and role of post-translational processing of CaV1.2, and the role of auxiliary proteins (such as A kinase anchoring proteins) involved in PKA regulation."
23948586
sparser
"Here, we show that the altered PKA-dependent phosphorylation of Cav1.2 is involved in this altered Ca 2+ handling both under basal conditions and following sympathetic stress."
39769475
reach
"Altered Protein Kinase A-Dependent Phosphorylation of Cav1.2 in Left Ventricular Myocardium from Cacna1c Haploinsufficient Rat Hearts."
39769475
sparser
"Second, we tested for altered PKA-dependent phosphorylation of Cav1.2."
39769475