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PKA phosphorylates CACNA1C. 21 / 27
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"Multiple mechanisms may contribute to the impaired β-adrenergic signaling in human HFrEF including altered PKA-dependent phosphorylation of Cav1.2 (and other Ca 2+ -regulating proteins)."
39769475
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"The results of this study convincingly show that isoprenaline-induced, PKA-dependent phosphorylation of Cav1.2 in LV myocardium fromCacna1c +/− rats is attenuated compared with WT, thus explaining the attenuated increases in sarcolemmal Ca 2+ influx."
39769475
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"PKA and CaMKII phosphorylate Cav1.2, RyR2, and PLB (among other proteins) to mediate the positive-inotropic and -lusitropic effects of sympathetic stimulation [4]."
37372947
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"By contrast, inCacna1c +/− myocytes, in the absence of β-adrenergic stimulation, there is greatly increased phosphorylation of both Cav1.2 and RyR2 at PKA sites S1928 (+110%) and S2808 (+120%), respectively, as depicted by some phosphorylated (P) residues (yellow circles)."
39769475
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"Activation of β-adrenergic receptors is known to lead to an increase of both L-Type Ca 2+ current (I CaL ) and slow delayed rectifier I Ks , via protein kinase A (PKA)-mediated phosphorylation of Cav1.2 and Kv7.1 channels, respectively."
35919344
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"The α1C subunit is phosphorylated by PKA in intact hippocampal neurons, and a two-fold increase in Ca 2+ influx has been observed in hippocampal neurons in old rats compared with adult rats, suggestive of increased PKA phosphorylation of Cav1.2 with aging."
35111050
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"Maximal PKA phosphorylation of Cav1.2 and RyR2 is reduced inCacna1c +/− compared to WT myocytes, leaving “empty”, i.e., non-phosphorylated serine residues (S1928 and S2808) in Cav1.2 and RyR2 (see white circles) and suggesting—once again—altered local regulation of cAMP and PKA-dependent phosphorylation in the dyadic cleft ofCacna1c +/− myocytes."
39769475
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"PKA and CaMKII phosphorylate Cav1.2, RyR2, and PLB (among other proteins) to mediate the positive-inotropic and -lusitropic effects of sympathetic stimulation [ xref ]."
37372947
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"Some of the cardinal debated issues include details of specific subunits and residues in CaV1.2 phosphorylated by PKA, the nature, extent, and role of post-translational processing of CaV1.2, and the role of auxiliary proteins (such as A kinase anchoring proteins) involved in PKA regulation."
23948586
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"In fact, perhaps the best known effect of β-AR stimulation in the heart is the activation of the cascade of Gαs protein and adenylyl cyclase that leads to the phosphorylation of the Cav1.2 channel by [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23811359
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"Catecholamines evoked increased cAMP responses, in turn activating phosphorylation of downstream targets RyR2, PLN, TnI, and Cav1.2 by PKA, increasing beating frequency and calcium kinetics [222] ."
34990783
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"Although the two phenomena occur simultaneously during physiological exercise, we separated them to dissect their relative contributions.Activation of β-adrenergic receptors is known to lead to an increase of both L-Type Ca current (I ) and slow delayed rectifier I , via protein kinase A (PKA)-mediated phosphorylation of Cav1.2 and Kv7.1 channels, respectively."
35919344
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"In conclusion, our study reveals altered PKA-dependent phosphorylation of Cav1.2 with elevated phosphorylation of serine-1928 under basal conditions and a diminished phosphorylation reserve during β-adrenergic stimulation."
39769475
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"Although phosphorylation of Cav1.2-pS1928 is not required for β-adrenoceptor stimulation of LTCC in mouse ventricular myocytes and HEK293 cells, xref it is a well-established Cav1.2 PKA phosphorylation site xref and phosphorylation of its α 1C subunit is relevant for channel function by increasing trafficking to the plasma membrane. xref In CON, the relative Cav1.2-pS1928 phosphorylation was significantly lower in cAF compared to SR and increased significantly after incubation with ISO or PF to levels seen in corresponding CONs ( xref and xref , xref ), pointing to significant contribution of PDE8 to LTCC dephosphorylation."
36810794
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"In conclusion, our study reveals altered PKA-dependent phosphorylation of Cav1.2 with elevated phosphorylation of serine-1928 under basal conditions and a diminished phosphorylation reserve during β-adrenergic stimulation."
39769475
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"Moreover, phosphospecific antibodies targeted against this site are available, enabling direct monitoring of the PKA-dependent phosphorylation of Cav1.2."
39769475
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"Here, we used such a phosphospecific antibody against S1928 in order to characterize the PKA-dependent phosphorylation of Cav1.2 and its potential role in the impaired sympathetic stress response ofCacna1c +/− rats."
39769475
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"Below, we will focus on two areas of calcium channel phosphorylation where recent progress stands out in our opinion — PKA dependent phosphorylation of L-type calcium channels and the identification o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27768908
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"Here, we show that the altered PKA-dependent phosphorylation of Cav1.2 is involved in this altered Ca 2+ handling both under basal conditions and following sympathetic stress."
39769475
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"Altered Protein Kinase A-Dependent Phosphorylation of Cav1.2 in Left Ventricular Myocardium from Cacna1c Haploinsufficient Rat Hearts."
39769475
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"Second, we tested for altered PKA-dependent phosphorylation of Cav1.2."
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