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USP10 deubiquitinates TBP. 3 / 3
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reach
"To test whether USP10 deubiquitinates and stabilizes TBP in mammalian cells, we overexpressed USP10 in 293T cells and monitored TBP ubiquitination levels in these cells."

reach
"To test whether USP10 deubiquitinates TBP through direct protein protein interactions, we used HA-TBP to co-IP with Flag-USP10 co-expressed in 293T cells."

"Huwe1 as an E3 ligase targeting TBP for K48-linked ubiquitination and proteasome-mediated degradation; We found that Huwe1 activity on TBP is antagonized by the deubiquitinase USP10, which protects TBP from degradation."