IndraLab

Statements

Peptide inhibits UCHL5. 4 / 4
| 4
reach
"The linear peptide, 1, inhibited UCH-L5 with an IC of 1.8 µM while the cyclic version, 2, inhibited UCH-L5 slightly better with an IC of 1.6 µM (Figure 4A)."
35720124
reach
"Hence we concluded that among the other members of the UCH family, the cyclic β-sheet peptide 2 inhibits UCH-L5 most efficiently.To further investigate whether the cyclic peptide 2 inhibits the activity of Rpn11, the metalloprotease DUBs found in the 19S cap of the 26S proteasome, we carried out a standard fluorescence polarization assay using a Ubiquitin-Fluorescence Polarization (Ub-FP) substrate containing Ub linked by an isopeptide bond to a TAMRA-labelled Ub peptide, comprising residues 41 to 54 of Ub (Supplementary Figure S3A)."
35720124
reach
"We first tested the length of the peptide that can inhibit UCH-L5 using a Ub-rhodamine substrate using a plate reader assay."
35720124
reach
"According to the data obtained, only the β-hairpin peptide encompassing residues 1 to 17 inhibited UCH-L5 at 50 µM concentration while other truncated peptides did not (Supplementary Figure S1)."
35720124