IndraLab

Statements

UCHL5 binds NFRKB. 47 / 47
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"The activity of UCH-L5 is enhanced when UCH-L5 combines with proteasome 19S regulatory subunit by Rpn13/Admr1 receptor and inhibited when UCH-L5 interacts with NFRKB."
29371935
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"And UCH-L5 also interacts with NFRKB in INO80 complex, but it remains unknown that whether UCH-L5 interacts with other components of INO80 complex or not."
29371935
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"UCHL5 interacts with and protects NFRKB (also known as INO80G) that is a component of INO80 chromatin remodeling complex from proteasomal degradation by removing K48 linked ubiquitin chain."
28764946
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"Importantly, two-hybrid assays detected an interaction between NFRKB and full-length Uch37, but not a truncated Uch37 that retains only the UCH domain."
18922472
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"However, the effect of DRAIC on the combination of UCHL5 and NFRKB was still unclarified, so we detected this combination in oeDRAIC and shDRAIC cell lines, whose results showed that oeDRAIC can significantly reduce the level of NFRKB coprecipitated by UCHL5, while shDRAIC can increase NFRKB binding with UCHL5, which confirmed the speculation that DRAIC may indirectly down-regulate the expression of NFRKB through affecting the deubiquitination induced by UCHL5."
32351584
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No evidence text available
18922472
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"While the activity of UCH-L5 is inhibited when UCH-L5 interacts with NFRKB, a component of INO80 complex, by combination and deubiquitination [ xref ]."
29371935
sparser
"Considering the deubiquitination function of UCHL5, we speculated that DRAIC might regulate the ubiquitination level of NFRKB by influencing the binding of UCHL5 and NFRKB, and then affect the protein expression of NFRKB."
32351584
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"Interestingly, immunoprecipitation studies from MG132 treated cells revealed that NFRKB interacted with UCHL5 less in the chromatin fraction than in the nucleoplasm, despite its interactions with INO80 being essentially equivalent in these two fractions (XREF_FIG; for input fractions, see XREF_SUPPLEMENTARY)."
25194926
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No evidence text available
18922472
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"At the same time, Co-IP assay confirmed the interaction between UCHL5 and NFRKB, which is in accord with the research by Nishi R [ xref ]."
32351584
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No evidence text available
28514442
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"Confirming the importance of the N-terminal 101 residues for stable association between Uch37 and NFRKB, Flag-NFRKB co-immunoprecipitated with endogenous Uch37 from HEK293 cells, whereas NFRKBDeltaN101, which lacked the first 101 amino acids, failed to do so (XREF_FIG)."
18922472
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"DRAIC is down-regulated in GC tissues and cell lines while its potential interacting molecules UCHL5 and NFRKB are up-regulated, and DRAIC is positively correlated with NFRKB protein instead of mRNA."
32351584
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"To define in more detail the interaction between Uch37 and NFRKB, we generated a series of fragments from the N-terminal third of NFRKB by in vitro translation using a bacterial cell-free system and tested their abilities to interact with GST-Uch37 in GST-pulldown assays."
18922472
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"And UCH-L5 also interacts with NFRKB in INO80 complex, but it remains unknown that whether UCH-L5 interacts with other components of INO80 complex or not."
29371935
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"Similarly, the results of bioinformatics analysis and Co-IP assay demonstrated that UCHL5 interacted with NFRKB, which is consistent with the research by Sahtoe DD et al. [XREF_BIBR]."
32351584
biogrid
No evidence text available
17721549
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"However, the effect of DRAIC on the combination of UCHL5 and NFRKB was still unclarified, so we detected this combination in oeDRAIC and shDRAIC cell lines, whose results showed that oeDRAIC can significantly reduce the level of NFRKB coprecipitated by UCHL5 (Fig.  xref d), while shDRAIC can increase NFRKB binding with UCHL5 (Fig.  xref e), which confirmed the speculation that DRAIC may indirectly down-regulate the expression of NFRKB through affecting the deubiquitination induced by UCHL5."
32351584
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No evidence text available
25416956
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"Confirming the importance of the N-terminal 101 residues for stable association between Uch37 and NFRKB, Flag-NFRKB coimmunoprecipitated with endogenous Uch37 from HEK293 cells, whereas NFRKBDeltaN101[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18922472
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"Nevertheless, the N101 fragment appears to account only partially for NFRKB binding to Uch37."
18922472
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No evidence text available
25702870
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No evidence text available
18922472
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"Recruitment to INO80 is mediated by the N-terminal domain of NFRKB (NFRKB NTD), which also binds the UCH37 CTD and, in sharp contrast to RPN13, further inhibits UCH37 activity."
25702872
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"Because hRpn13 and NFRKB interact with Uch37 in the proteasome and hINO80, respectively, we asked whether the isolated proteins bind to Uch37 in a mutually exclusive fashion."
18922472
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"The activity of UCH-L5 is enhanced when UCH-L5 combines with proteasome 19S regulatory subunit by Rpn13 and Admr1 receptor and inhibited when UCH-L5 interacts with NFRKB."
29371935
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"At the same time, Co-IP assay confirmed the interaction between UCHL5 and NFRKB, which is in accord with the research by Nishi R [XREF_BIBR]."
32351584
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"The authors found that UCH-L5 interacted with and stabilized NFRKB."
27516771
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"Nevertheless, the N101 fragment appears to account only partially for NFRKB binding to Uch37."
18922472
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No evidence text available
25702870
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"DRAIC combined with UCHL5 and attenuated binding of UCHL5 and NFRKB, meanwhile promoting the degradation of NFRKB via ubiquitination, and then inhibited the proliferation and metastasis of GC cells, which can be rescued by oeNFRKB."
32351584
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"The above bioinformatics results suggested that DRAIC, UCHL5 and NFRKB may interact with each other and exert biological effects in GC."
32351584
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"Previous work has established that UCHL5 is a component of both the proteasome and the INO80 chromatin remodeling complex; these complexes being mediated via UCHL5 interactions with hRPN13 and NFRKB ( n uclear f actor r elated to κ B- b inding protein), respectively ( xref ) xref , xref , xref ."
25194926
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No evidence text available
25194926
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"This is similar to what was previously observed for the interaction between Uch37 and hRpn13, the proteasome subunit that binds Uch37 via the C-terminal tail to recruit it to the 19S RP.To define in m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18922472
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"The association of Uch37 with the DNA-binding NFRKB subunit of INO80 suggests that Uch37’s role in gene regulation could be restricted to specific genes that contain NFRKB binding sites."
18922460
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No evidence text available
25702872
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"On the other hand, INO80G DEU binds to UCH-L5 and uses ULD conformational flexibility to dock its unique inhibitory FRF hairpin into the Leu38 pocket."
25702870
sparser
"To define in more detail the interaction between Uch37 and NFRKB, we generated a series of fragments from the N-terminal third of NFRKB by in vitro translation using a bacterial cell-free system and t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18922472
sparser
"Interestingly, immunoprecipitation studies from MG132-treated cells revealed that NFRKB interacted with UCHL5 less in the chromatin fraction than in the nucleoplasm, despite its interactions with INO80 being essentially equivalent in these two fractions ( xref ; for input fractions, see xref )."
25194926
sparser
"Similarly, the results of bioinformatics analysis (Fig.  xref a bottom) and Co-IP assay (Fig.  xref c) demonstrated that UCHL5 interacted with NFRKB, which is consistent with the research by Sahtoe DD et al. [ xref ]."
32351584
sparser
"Unexpectedly, however, both the longer N465 fragment and full-length NFRKB blocked formation of the Uch37-UbVS adduct."
18922472
reach
"Considering the deubiquitination function of UCHL5, we speculated that DRAIC might regulate the ubiquitination level of NFRKB by influencing the binding of UCHL5 and NFRKB, and then affect the protein expression of NFRKB."
32351584
sparser
"DRAIC combined with UCHL5 and attenuated binding of UCHL5 and NFRKB, meanwhile promoting the degradation of NFRKB via ubiquitination, and then inhibited the proliferation and metastasis of GC cells, which can be rescued by oeNFRKB."
32351584
biogrid
No evidence text available
19615732
biogrid
No evidence text available
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