IndraLab

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CYLD decreases the amount of CYLD. 7 / 9
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"An adeno associated virus (AAV) vector encoding CYLD was used to suppress CYLD expression by being intratracheally instilled in rats 7 days before MCT-AV treatment."

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"Adenoviral over-expression of CYLD shRNA (20 MOI) completely inhibited CYLD protein expression."

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"In vitro cell proliferation was measured and the result shows that CYLD knockout causes cell proliferation enhancement and CYLD overexpression causes cell proliferation suppression (XREF_FIG C, D)."

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"Furthermore, protein expression of SOX2 and EGFR was reduced while there was a greater increase in CYLD expression following treatment with sh-SOX2, while additional miR-222-5p agomir only decreased CYLD protein expression without affecting SOX2 and EGFR expression (Figure 7E, 7F)."

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"Adenoviral over-expression of CYLD shRNA dose-dependently inhibited CYLD mRNA expression with an efficacy of> 90% knockdown of endogenous CYLD at dose of 10 multiplicity of infection (MOI) in HK-2 cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Western blotting and a dual-luciferase reporter assay were used to verify that miR-1288 targets CYLD by interacting with the 3 ' UTR of CYLD to reduce CYLD expression."

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"To further confirm CYLD as a target for human melanoma, specific si-RNAs of CYLD were used to inhibit CYLD expression in both melanoma cells A375 and WM35, respectively."