IndraLab

Statements

SNX27 binds OTULIN. 27 / 27
| 8 19
sparser
"An X-ray crystal structure of OTULIN-SNX27 PDZ revealed a second interface in addition to the canonical PDZ-PDZbm interaction."
33937239
sparser
"Therefore, we concluded that the interaction between OTULIN and SNX27 has mutual effects on each other."
| PMC
sparser
"To further study the interaction between OTULIN and SNX27, we constituted the interaction assay using constructs containing deletions and mutations of SNX27 and OTULIN."
| PMC
sparser
"Here, we show that the interaction between SNX27 and Met1 linkage is mediated by OTULIN, consistent with the recent reported interaction of SNX27 and OTULIN [ xref ]."
| PMC
sparser
"By solving the structure of the OTU domain in complex with the PDZ domain, we demonstrate that a second interface contributes to the selective, high affinity interaction of OTULIN and SNX27."
31541095
reach
"By solving the structure of the OTU domain in complex with the PDZ domain, we demonstrate that a second interface contributes to the selective, high affinity interaction of OTULIN and SNX27."
31541095
sparser
"SNX27 interacts with OTULIN (OTU Deubiquitinase With Linear Linkage Specificity) that specifically hydrolyzes methionine1 (Met1)-linked ubiquitin chains."
33374212
reach
"Therefore, we concluded that the interaction between OTULIN and SNX27 has mutual effects on each other."
| PMC
sparser
"Mechanistic studies showed that, upon TNFα stimulation, OTULIN-SNX27 is localised to membrane-associated TNF receptor complex, where OTULIN deubiquitinates the linear polyubiquitin chain that formed by the LUBAC complex."
34315543
sparser
"Therefore, we speculated that when cells are undergoing TNFα stimulation, the interaction between OTULIN and SNX27 will be transiently increased, which subsequently decreases the loading of other SNX27 dependent cargo proteins [ xref ]."
| PMC
sparser
"FIGURE 5: Sorting nexin 27 (SNX27) binding to retromer and OTULIN."
33937239
sparser
"To determine the underlying mechanisms by which SNX27 regulates linear polyubiquitin chain formation by interacting with OUTLIN, we first investigated the interaction of SNX27 and OTULIN upon TNFα-stimulation."
| PMC
sparser
"As shown in Fig.  xref A, the interaction between endogenous SNX27 and OTULIN was upregulated when cells were treated with TNFα for 5 min."
| PMC
sparser
"Using recombinantly expressed proteins, we demonstrated that SNX27 directly interacts with OTULIN (Additional file xref : Figure S2D)."
| PMC
reach
"In summary, we showed that the interaction of SNX27 and OTULIN potentiates the translocation of OTULIN to TNFalpha receptor complex."
| PMC
sparser
"In summary, we showed that the interaction of SNX27 and OTULIN potentiates the translocation of OTULIN to TNFα receptor complex."
| PMC
sparser
"Dysregulation of the LUBAC-independent OTULIN-SNX27 complex could also be involved [ xref ]."
32231246
reach
"Using recombinantly expressed proteins, we demonstrated that SNX27 directly interacts with OTULIN (Additional file 2 : Figure S2D)."
| PMC
sparser
"Mechanistic studies showed that, upon TNFα stimulation, OTULIN-SNX27 is localised to membrane-associated TNF receptor complex, where OTULIN deubiquitinates the linear polyubiquitin chain that formed by the LUBAC complex."
| PMC
sparser
"Structural data suggest the interaction of SNX27 PDZ with retromer/cargo or OTULIN is mutually exclusive."
33937239
reach
"Therefore, we speculated that when cells are undergoing TNFalpha stimulation, the interaction between OTULIN and SNX27 will be transiently increased, which subsequently decreases the loading of other SNX27 dependent cargo proteins [XREF_BIBR]."
| PMC
sparser
"Sorting nexin 27 (SNX27) binding to retromer and OTULIN."
33937239
reach
"To determine the underlying mechanisms by which SNX27 regulates linear polyubiquitin chain formation by interacting with OUTLIN, we first investigated the interaction of SNX27 and OTULIN upon TNFalpha stimulation."
| PMC
reach
"XREF_FIG A, the interaction between endogenous SNX27 and OTULIN was upregulated when cells were treated with TNFalpha for 5min."
| PMC
sparser
"Because the SNX27 and OTULIN antibodies are not suitable for endogenous immunoprecipitation, we alternatively showed that endogenous SNX27 and OTULIN interact with ectopic expressed OTULIN and SNX27 (Additional file xref : Figure S2A–S2C)."
| PMC
reach
"To further study the interaction between OTULIN and SNX27, we constituted the interaction assay using constructs containing deletions and mutations of SNX27 and OTULIN."
| PMC
sparser
"Immunoprecipitation in 293T cells and in vitro pull-down assays using bacterial recombinant proteins indicated that the direct interaction between SNX27 and OTULIN was mediated by the PDZ domain of SNX27 and the last 6 amino acids of OTULIN (Additional file xref : Figure S2E–G), respectively."
| PMC