IndraLab

"ANG II mediated BK channel degradation reduced BK currents in isolated myocytes and functional responses to iberiotoxin, a BK channel blocker, and NS1619, a BK activator, in pressurized (60 mmHg) cerebral arteries."

"Ang II inhibits BK channel function through activation of its downstream pathways."

"To determine the role of caveolae in Ang II signal transduction, we examined the effects of cav-1 knockdown in rat coronary arterial SMC using siRNA on BK channel inhibition by Ang II. xref shows the protein expression of cav-1 in coronary arterial SMC 48 h after transfection with cav-1 siRNA at 0, 20, 40, 60, and 100 nmol/L, and control siRNA at 100 nmol/L. Cav-1 siRNA at 40 nmol/L or higher significantly suppressed cav-1 expression by 80% to 90%, compared with control siRNA. xref shows the time course of whole-cell K + currents in rat coronary arterial SMC 48 h after transfection with 100 nmol/L cav-1 siRNA or 100 nmol/L control siRNA, in response to 2 μmol/L Ang II and 0.1 μmol/L IBTX."