IndraLab

Statements

EGF decreases the amount of EGFR. 67 / 89
4 | 63
reach
"Consistent with these data, this study demonstrates that systemic administration of EGF prevents aberrant overexpression of EGF-R in the intestine of mice with pneumonia."
21701422
reach
"The clonal line secreting high levels of sEGF displayed a 75% reduction in EGFR levels, which was not reduced further by the addition of exogenous EGF, indicating a maximal level of receptor downregulation."
9832559
reach
"As can be seen, PMA treatment did indeed lead to inhibition of the loss of EGFR induced by EGF."
24244711
reach
"EGF stimulation reduced total EGFR levels."
22830443
reach
"Moreover, when LOX was depleted, EGF stimulated more rapid loss of EGFR from the cell surface (XREF_FIG; XREF_SUPPLEMENTARY)."
28416796
reach
"However, EGF, but not AREG, further decreased the total EGFR amount (XREF_FIG, lower panel), consistent with the known ability of EGF to cause rapid EGFR down-modulation [XREF_BIBR, XREF_BIBR, XREF_BIBR] and suggesting that AREG activated EGFR might escape this negative regulation."
27709733
reach
"Instead of repressing cellular responses to EGF, SHIP2 causes an increase in EGF induced signaling by elevating the level of the EGF receptor (EGFR)."
24302498
reach
"It has been previously reported that EGF activation of EGFR leads to EGFR endocytosis and routing for degradation rather than recycling to the cell surface [47], which explains our finding that EGF stimulation diminished the level of EGFR and HLA class I/EGFR association in BxPC-3 cells."
35303241
reach
"Reduction of CD82 expression by RNA interference together with depletion of glycosphingolipids with P4 significantly enhanced cell motility and increased the expression of EGFR and its phosphorylation at Tyr1173 in response to EGF."
23968914
reach
"Therefore, a 2-fold reduction of a surface EGFr expression by EGF induced intemalization may result in significant reduction in CT sensitivity."
8630095
reach
"EGF polyplexes diminished cell surface expression of EGFR for up to 4 hr, whereas GE11 polyplexes did not."
21644815
reach
"Indeed, whereas EGF strongly reduced the levels of EGFR at 30 and 90minutes following stimulation of wild-type cells, it had no detectable effect upon the EGFR Gly428Asp levels inthe patient cell line."
26436111
bel
"As expected, by using western blotting, we observed upregulation of the respective protein (LRIG1) 2 h following EGF treatment, concurrent with gradual disappearance of the respective receptor, ErbB-1 (Figure 1C)."
15282549
reach
"EGFR protein expression was also decreased by short-term EGF exposure by approximately 30% in both male and female FDLE cells (p < 0.05; p < 0.001; Fig. 5f)."
34354180
reach
"In contrast, siRNA mediated downregulation of endogenous (RAEW) and transfected (H7/MVP) MVP under serum starved, EGF stimulated conditions resulted in decreased EGFR expression and reduced AKT and S6 phosphorylation."
24243798
reach
"After inhibition of EGFR or STAT3, the proliferation of ASCs was significantly inhibited, and Western blotting showed EGF was able to markedly increase the expression of EGFR and STAT3."
28746211
reach
"EGF stimulation decreased the expression level of EGFR in EC109 cells and TT cells, but not in A431 cells."
24820097
reach
"Similarly, EGFR protein expression was reduced by EGF in fetal lung fibroblasts, while no sex-specific expression was observed in the latter cell type."
34354180
reach
"An EGFR inhibitor, AG1478, abrogated the PSA suppression effect by EGF, in concurrence with the suppression of tyro phosphorylation levels of EGFR."
16472761
reach
"Collectively, these results show that AT-II induces translocation of both the AT 1A R and the heterologous receptor, EGFR to the pericentrion with the functional consequence of inhibiting the loss of EGFR induced by EGF."
24244711
reach
"XREF_BIBR - XREF_BIBR CMTM3 could inhibit epidermal growth factor (EGF)-mediated migration and STAT3 signaling, suppress the expression of EGFR through promoting the degradation of EGFR in gastric cancer cells."
32606785
reach
"Next, we analyzed expression levels of phosphorylated ERK1/2 and compared these results with EGFR (HER-1 and ErbB1) and HER2 and neu (ErbB2) expression levels, as EGFR conducts signals through Ras-Raf-MAPK pathway; gefitinib inhibited phosphorylation of ERK1/2 by EGF addition in cell lines with detectable and undetectable EGFR expression."
15492792
reach
"In FDLE cells cultured in SF-Med no sex-specific protein expression of EGFR was observed, and EGF application significantly downregulated EGFR protein expression after 24 h in both sexes (p < 0.001; Fig. 4b)."
34354180
reach
"Diminished phospho-EGFR expression was confirmed in control (Pten fl/fl) epithelium as a response to erlotinib treatment, and importantly, EGF ligand mRNA expression is not reduced in isolated PTEN-null (Fsp-cre; Pten fl/fl) MMFs with in vitro EGFR inhibition supporting a direct epithelial specific response."
30018330
reach
"4(d) and S6] demonstrated significantly lower levels of EGFR expression in cells exposed to an increasing concentration of EGF as compared to the SFM control, suggesting that loss of the EGFR in response to increasing EGF resulted in pathway insensitivity."
34765858
reach
"EGF induced expression of the STAT3 target gene HIF1alpha was abolished by cetuximab in HNSCC cells expressing wild-type EGFR under hypoxic conditions, but not in EGFRvIII expressing HNSCC cells [XREF_BIBR, XREF_BIBR]."
21776391
reach
"Additionally, EGF treatment decreased the EGFR protein levels in shControl cells, consistent with degradation of the receptor in response to EGF stimulation (XREF_FIG)."
24166502
reach
"EGF increased p-EGFR and p-Akt S473, but decreased the amount of total EGFR."
22764099
reach
"In contrast, the EGF dependent phosphorylation of p90RSK (XREF_FIG) and FLNA (XREF_FIG) was significantly decreased at all time points in the cells (clone 9-1) expressing low levels of EGFR."
26053065
reach
"As shown in XREF_FIG, EGF induced a time dependent reduction in ErbB1 levels which was significantly lower in cells overexpressing nucleolin."
18523588
reach
"Pretreatment with sulindac metabolites for 24 h blocked EGF induced phosphorylation of both EGFR and ERK1/2 and decreased total EGFR protein expression."
16138927
reach
"FTs (14 days old) were incubated in media with the addition of external EGF (20 ng/ml) for 24 h. Western blot analysis revealead i) that both MCF-7 and H460 cell lines have a functional EGFR signaling axis since as expected addition of external EGF donwregulated the expression of the EGFR (XREF_FIG), ii) that while EGF was able to downregulate the expression of EGFR, no significant effect on other proteins such as Nanog, Oct4, Sox2, AKT and p-AKT was observed (XREF_FIG)."
27925198
reach
"This finding is consistent with the observation that EGF, at nm concentrations, downregulates EGF-R expression in primary human placental cells (Lai and Guyda, 1984; Bhaumick and Bala, 1991), purified[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9778120
reach
"Consistently, EGF stimulation reduced the membrane EGFR level in control NCI-H157 cells but not in NCI-H157 cells overexpressing TRIB3; additionally, monensin reduced the EGFR membrane level in TRIB3-overexpressing NCI-H157 cells (Supplementary Fig. 2b, c)."
32694521
reach
"Depletion of EGF and TIMPI in cell culture slowed down cell growth and reduced EGF receptor expression, respectively."
11805517
reach
"Especially, EGFR expression was down-regulated by absence of EGF stimulation and it might be related with expression of Sec61beta protein that was regulated by EGF."
28785734
reach
"We report, for the first time, that sulindac metabolites block both basal and EGF induced phosphorylation of EGFR and downregulate total EGFR protein expression."
16138927
reach
"In all three cell lines EGF reduced total EGFR protein levels more than TGF-alpha, but this was associated with a greater increase in de novo protein synthesis with EGF compared to TGF-alpha."
10360641
reach
"This reduction of EGF stimulated endocytic signalosomes in Cdc42 iKO crypt cells echoed significant decreases in levels of p-ErbB1 (Y1068) and p-ErbB2 (Y1221/1222), active forms of major EGFRs in Cdc42 iKO IECs (XREF_FIG)."
32686657
reach
"Lenferink et al. [XREF_BIBR] demonstrated that EGF stimulation rapidly diminishes EGFR expression in contrast to TGF-alpha which caused reappearance of EGFR presumably due to recycling of endocytosed receptors."
25866477
reach
"In blastocysts, EGF significantly decreased EGFR expression in those cultured singly or in groups (XREF_FIG)."
25985792
bel
"Our experiments consistently showed EGFR degradation following EGF, but not arsenic treatment (Figs. 1 and 2, row 1 EGFR)."
19168569
reach
"EGF application again significantly reduced EGFR protein levels in both male and female fibroblasts (p < 0.01; Fig. 8e)."
34354180
reach
"Stimulation of cells with EGF for 60 min did not reduce the membrane EGFR level in control A549 cells but did reduce the level in control A549 cells pretreated with monensin, a recycling inhibitor, indicating that EGFR is recycled back to the cell surface after 60 min of EGF treatment."
32694521
reach
"Exogenous EGF down-regulated the expression of functional EGF-R, selectively in transformed cells."
1601524
reach
"Firstly, sustained activation of PKC by AT-II inhibited the loss of EGFR induced by EGF treatment coincident with co-sequestration of EGFR and the AT 1A R in the pericentrion."
24244711
reach
"EGF downregulates the expression of EGFR in FTs."
27925198
reach
"Both, the mRNA and protein expression of EGFR was strongly decreased by EGF in both sexes."
34354180
reach
"The influence of EGF on EGFR expression was assessed and found to decrease the expression of EGFR transcript in morulae cultured singly and blastocysts cultured either individually or in groups."
25985792
reach
"Importantly, the inhibition of cell viability was abolished by EGF stimulation in cancer cells expressing high levels of EGFR."
19948216
bel
"After 10 min of EGF treatment, EGFR levels dropped 2-fold in cells transfected with the control morpholino (Fig. 6, A and B), whereas cells depleted of Rin1 showed very little receptor loss following EGF stimulation."
19570984
reach
"The protein level of EGFR was decreased by EGF treatment, and this was thought to represent receptor endocytosis and degradation."
16472761
reach
"In this paper, we show that CMTM3 suppresses epidermal growth factor (EGF)-mediated migration and STAT3 signaling, downregulates EGFR expression via accelerating EGFR degradation in gastric cancer cells."
27867015
bel
"EGFR down-regulation (internalization and degradation) in response to EGF (25, 62).....The long term, 24 h, treatment of cells with EGF led to depletion of EGFR levels by more than 90% and abolished the NE-initiated as well as EGF-dependent ERK activation."
11889128
reach
"EGF induced EGFR activation and mTOR activation in EGFR expressing H460 and A549 NSCLC cell lines was inhibited."
29234489
reach
"Real time PCR confirmed that treatment with B6H12 alone or in the presence of EGF eliminated detectable EGF and EGFR transcript expression, whereas treatment with isotype control antibody in the presence or absence of EGF did not significantly alter mRNA expression of EGF and EGFR."
26840086
reach
"EGF exposure reduced total EGFR levels, consistent with previous studies showing that EGF : EGFR engagement promotes EGFR internalization, ubiquitination, and degradation via the lysosomal pathway."
25392346
reach
"Indeed, EGF stimulation triggered loss of cellular EGFR and gain of EGFR-EVs."
30017201
reach
"As detected by immunohistochemical staining, the expression of EGF receptor in the epidermis was reduced by exogenous EGF."
7798626
reach
"Quantitative evaluation of jejunal EGF-R mRNA (n = 8-15/group) or protein levels (n = 4-5/group) revealed similar patterns, where septic mice had significantly higher levels of intestinal EGF-R compared to shams, with exogenous EGF treatment after the onset of sepsis reducing intestinal EGF-R expression (XREF_FIG)."
18004230
reach
"Expression of the mature form of EGF causes a marked decrease in EGFR expression, but expression of either form of TGFalpha does not (XREF_FIG, lower panel)."
22260327
reach
"One possible explanation for the loss of EGFR in response to EGF is that the receptor was internalized and degraded."
34765858
reach
"This revealed that ligand induced receptor loss was significantly greater for EGFR T669A than for WT EGFR at all EGF stimulation time points tested and that the degree of loss was progressively greater for EGFR T669A vs. WT EGFR with increasing duration of EGF treatment; 3h of EGF exposure caused nearly 50% loss of WT EGFR and> 90% loss of EGFR T669A."
18762250
reach
"In accordance, EGF also reduced EGFR mRNA expression in male and female cells by approximately 80% (p < 0.001; Fig. 4c), and EGFR mRNA expression was not sex-specific."
34354180
reach
"Using HT29 cells, EGF significantly (p < 0.01) decreased EGFR levels in vector control and sh-NDRG1 cells relative to these cells treated without EGF, while having no significant (p> 0.05) effect on EGFR levels in NDRG1 over-expressing or sh-control cells (XREF_SUPPLEMENTARY)."
25860930
reach
"Moreover, EGF seemed to down-regulate EGFR expression, similar as reported for EGFR amplified GSCs XREF_BIBR, XREF_BIBR, suggesting that GSCs should be cultured in stem cell medium supplemented with bFGF only to better preserve the original GSC genotype."
24294177
reach
"Clonal off-target effects as an explanation for the observed phenotype could be excluded with high confidence based on the close agreement of observations with two different cell lines.In summary, the lack of GABARAP, either alone or in combination with GABARAPL2, was consistently and significantly associated with decreased total EGFR protein levels in response to EGF treatment in both Huh7.5 and HEK293 cells, whereas the presence of GABARAP in general was associated with higher EGFR total levels."
32456010