IndraLab

Statements

CYLD binds Flag. 6 / 6
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"The result showed that Myc-p18 was detected in the Flag-CYLD immunoprecipitates (Fig. xref )."
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"We found that the anti-Flag antibody, but not the anti-IgG antibody, pulled down Flag-CYLD, as well as NDRG1, from CNE2 cells."
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"To further confirm the physiological interaction, Flag-CYLD and Myc-p18 were transfected into 293T cells, and co-immunoprecipitation was performed using a Flag antibody."
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"In vitro , Flag-CYLD was pulled down by GST-ERK1, but not GST ( xref ), indicating that a direct interaction between CYLD and ERK1."
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"To investigate which type of poly-Ub chain on p18 is cleaved by CYLD, we used 293T cells transfected with Myc-p18, Flag-CYLD, together with HA-tagged ubiquitin mutants (K6, K11, K27, K29, K33, K48, or K63)."
33654169
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"The interaction of Flag-CYLD and Myc-RIP1 was taken as positive control."
33654169