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CTNNB1 binds LEF1. 882 / 882
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"Moreover, when the endogenous Smad7 was repressed by specific small interfering RNA, TGF-beta-induced increase of activated p38, Akt phosphorylated on Ser473, glycogen synthase kinase 3beta phosphorylated on Ser9 was prevented, as well as the TGF-beta-induced association between beta-catenin and LEF1."
15684397
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"This model is consistent with our observations that β-catenin and LEF1 readily form heterotypic condensates in vitro ( xref ), while mutation of the terminal IDR aromatic residues (aroNC) reduces heterotypic condensation ( xref )."
39316611
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"The significance of this was poorly understood until yeast two hybrid studies revealed that the architectural HMG-box transcription factors LEF-1 and XTCF-3 interact with beta-catenin (Behrens et al.,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10495268
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"Active Wnt/β-catenin signalling induces the stabilization of cytosolic β-catenin, which then associates with lymphoid enhancer factor and T-cell factor, such as transcription factor 7-like 2 (TCF7L2 or TCF4) to form a transcription complex that activates Wnt target genes."
22767436
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"However, unlike embryonic development in which uniform upper dermal Wnt signaling precedes hair morphogenesis ( xref ; xref ; xref ) we could not detect Wnt activity in the upper dermis prior to hair germ formation by either β-catenin or LEF-1 immunohistochemistry or with a Wnt-responsive reporter ( xref , data not shown)."
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"Furthermore, endogenous co-IP with CTNNB1 antibody exhibited that, LSM12 knockdown repressed the binding of CTNNB1 to LEF1 and TCF1 (Figs."
37303493
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"To quantitatively measure the ability of GATA4 to block interaction between β-catenin and LEF1/TCF1, we turned to bimolecular fluorescence complementation assay 34 by fusing β-catenin and LEF1/TCF1 to N- and C-terminal half of firefly luciferase respectively, such that when these fusion proteins were expressed in HepG2 cells, luciferase activity is a direct readout for interaction between β-catenin and LEF1/TCF1."
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"Inspection of the crystal structures of the β-catenin bound with TCF, LEF1 and TCF4 reveals that all the peptide motifs bound to the binding site 3 of β-catenin are enriched in Asp and Glu, and these negatively charged residues appear to form very favorable interaction with Q302 and R376 of β-catenin (Fig. 6d)."
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"There is evidence that β-catenin can act as a nuclear import receptor to induce the nuclear translocation of Lef1 xref , indicating a close interaction between Lef1 and β-catenin."
23630438
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"In addition, recent data demonstrates that at the onset of anagen, HG exchanging Tcf3/Tcf4 for Lef1 at the super-enhancers, and then Lef1 forms the nuclear complex with β-catenin after activation of WNT signaling (as a consequence of BMP inhibition) which drives HG committed progenitors ( xref ; xref ; xref )."
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"Previous studies clarified that the N-terminal end of lef-1 binds to β-catenin [24,25,28] ."
15936755
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"These results indicate that β-catenin’s IDRs amplify the association of β-catenin and LEF1 driven by structured protein-protein interactions."
39316611
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"In the nucleus, β-catenin binds and activates the lymphoid enhancer factor (LEF)/T cell factor (TCF) transcription factor to regulate the expression of Wnt target genes ( xref )."
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"In the canonical Wnt signaling pathway, the beta-catenin in the nucleus forms a complex with LEF1 to activate transcription of the target gene [XREF_BIBR]."
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"Then, authors showed that these aromatic amino acids are important in forming BMC droplet with Lef-1 IDR, and this beta-catenin-Lef-1 BMC greatly accelerates Wnt signaling."
39316611
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"Β-catenin which is then subsequently translocate to the nucleus interacts with T-cell factor and lymphoid enhancer factor (TCF/LEF)-family transcription factors ( xref ; xref ), initiating transcription of a set of pro-inflammatory, proliferative genes and oncogenes, such as CCND1 and MYC ( xref )."
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"Β-catenin enters the nucleus from cell membrane and cytoplasm, and binds to transcription factors LEF1 and TCF1 to form a transcription factor complex, which promotes the expression of downstream gene[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31887919
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"She found that the HMG box protein TCF-1 and Lef-1 can associate with beta -catenin, and can bind to target DNA as a complex [16]."
9061012
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No evidence text available
16510875
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"Both Wnt signalling and Shh signalling are inappropriately activated in epidermal tumours [ 2–4,39 • ], and it therefore comes as a surprise that human sebaceous gland tumours harbour LEF1 mutations t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Nuclear translocation of beta-catenin binds to the DNA binding proteins Tcf and Lef1 that subsequently regulates target genes including CyclinD1 important in proliferation."
22391317
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"Further, while it is known that beta-catenin and LEF1 are transcriptional coactivators and form a complex within the nucleus, and that beta-catenin co-activates LEF1 transcription, these proteins are rarely studied together in context of disease."
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"TGF-beta1 induced nuclear Smad3, beta, and catenin complex, but not beta-catenin and LEF -1 complex or TOP-flash activity, during EMT of C1.1 (renal tubular epithelial) cells."
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"EpICD directly interacts with beta-catenin and lymphoid enhancer factor (LEF) [ xref ]."
31547062
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"As described in the Discussion section, authors introduced S33Y mutation to stabilize beta-catenin for focusing the transcriptional activity by beta-catenin-Lef-1 BMC."
39316611
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"In the nucleus, beta-catenin interacts with the transcription factor LEF-1, which activates transcription of the target genes [XREF_BIBR]."
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"In mouse palate development, TGFβ3 activates transcription of the β-catenin binding protein Lef-1 via the Smad pathway and a dominant negative mutant of Lef-1 blocks TGFβ3 stimulated palatal EMT ( Naw[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17026982
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"Then, β-catenin enters the nucleus and forms a complex with CBP, TCF4, and LEF1 to regulate the expression of target genes, such as Runx-2 (Dan et al., 2023)."
37680712
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"Accumulated unphosphorylated β-catenin translocates to the nucleus and eventually binds to the lymphoid enhancer factor/T cell factor (LEF/TCF) transcription factors to exert effects on downstream gene transcription."
29361730
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"In Fig. 2, authors introduced substitutions of aromatic amino acids only in beta-catenin IDRs to confirming BMC mimicking interaction between beta-catenin and Lef-1."
39316611
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"Low levels of YREG1 were important for stabilizing the interaction between LEF-1 and beta-catenin, while elevated levels inhibited EMT by downregulating LEF-1 transcriptional activity."
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"The same is true for mammalian cells, where FHL2 does not interfere with the β-catenin–LEF-1 interaction (unpublished data)."
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"Moreover, a possible anti-cancer target of sanguinarine in vitro is the interaction between β-catenin and LEF1, based on a high-throughput sequencing assay ( xref )."
37841927
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"As shown in Fig. 2 , CTNNB1 binds LEF1, while both of them can interact with HDAC1 [9] ."
22819921
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"These results are in accordance with previous studies showing that when CTNNB1 is bound to LEF1 it induces the transcription of LEF1 dependent genes and disturbs the binding of LEF1 to HDAC1 [9] ."
22819921
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"Whereas ectoptic WT β-catenin bound p300 upon exposure of the cells to both glucose and LiCl (like endogenous β-catenin), conditions that promote LEF-1 and β-catenin interaction and nuclear accumulati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
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"Thus, the LEF-1 and β-catenin interaction promoted by the combination of glucose and LiCl appears critical for stabilizing p300 interaction and nuclear retention."
23273980
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"In the classical pathway of Wnt/β-catenin signaling, β-catenin binds to transcription factor Lef1/TCF family [1] ."
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"Subsequently β-catenin interacts with members of the lymphoid enhancer factor 1/T-cell factor (LEF1/TCF) family, resulting in generation of a functional transcription factor complex and the expression of downstream target genes. [ xref ] Non-canonical Wnt ligands bind to FZD family receptors, and ROR2 and RYK co-receptors.[ xref – xref ] This signaling is mainly involved in cytoskeletal reorganization during cancer cell invasion and metastasis.[ xref ][ xref ] At present, five Wnt antagonist families have been described, namely, secreted frizzled-related protein (sFRP), Wnt inhibitory factor 1 (Wif1), Xenopus Cerberus, Wise and Dickkopf (DKK) families.[ xref ]"
19562778
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"As shown in Fig. 3, β-catenin binds to LEF-1 to inhibit CDH1 transcription and cause EMT during gastrulation [47]."
39959483
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"In addition, CK2 has been shown to regulate the interaction and transactivation activity of a beta-catenin and LEF -1 complex through phosphorylation of LEF-1 at S42, S61 [XREF_BIBR; XREF_BIBR]."
19565571
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"At the GIP , GCG , and cyclin D promoters, both LEF-1 and β-catenin were poorly bound in the absence of LiCl."
23273980
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"Single treatment with glucose or LiCl alone induced only minor increases in the binding of both LEF-1 and β-catenin."
23273980
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"Mechanistically, TCF4 and LEF1 forms a complex with beta-catenin to bind its target gene promoters and promote cancer cell proliferation 28."
29765046
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"The interaction between Lef1 and β-catenin was clearly detected ( Fig. 4 B, lower)."
17445771
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"Increased binding of both LEF-1 and β-catenin at the cyclin D promoter follows combined treatment with glucose and LiCl in HT-29 colon cancer cells."
23273980
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"The Lef1, high mobility group (HMG) family transcription factor, interacts with beta-catenin and is a nuclear effector of Wnt1."
26537192
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"We confirmed the β-catenin and Lef1 interaction by overexpressing HA-tagged Lef1 alone or β-catenin together."
17445771
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No evidence text available
33431799
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No evidence text available
33431799
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No evidence text available
11835406
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"Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer binding factor) interacts with beta-catenin."
8757136
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"Because LEF-1 possesses its own NLS ( Huber et al., 1996 ) and is able to interact with β-catenin, we asked whether glucose favored LEF-1 and β-catenin interaction as a possible mechanism for the nucl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
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"Immunoprecipitation experiments indicated that LiCl and glucose cooperate to selectively favor nuclear LEF-1 and β-catenin interaction, and neither glucose nor LiCl alone allowed complex formation ( F[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
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"The mutated β-catenin interacts with LEF1 in the nucleus, making LEF1 expression a potentially useful candidate for demonstration of WNT activation and diagnosis of DPN. xref , xref , xref "
37462205
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"Potential intracellular Wnt regulators could target components of the destruction complex [ 62 ] or compounds having an activating effect on the interaction between β-catenin and Tcf/Lef1 [ 63 ]."
32569871
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"First, it increases p300 expression and enhances its interactions with LEF-1 and β-catenin; second, it inhibits sirtuin deacetylase activity."
23273980
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"K354 lies within the region absent in the Δ19 mutant, a region previously identified as being critical for β-catenin interaction with LEF-1 ( von Kries et al., 2000 )."
23273980
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"Although the precise molecular mechanisms have not been explored here, we view it as probable that SIRT1 acts as a competitor for interaction between LEF-1 and β-catenin."
23273980
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"Although SIRT1 may play a role via stable and direct binding to LEF-1 or β-catenin, it is also clear that its catalytic activity is particularly important."
23273980
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"have demonstrated that in vitro transcriptional activation by recombinant beta-catenin and LEF -1 complexes requires ubiquitin."
21118991
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"On the other hand, a strong binding of beta-catenin to cadherin, rather than to LEF-1, may result in its cytoplasmic sequestration and the inhibition of transactivation by it."
9628899
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"In the classical Wnt/β-catenin pathway, β-catenin accumulates in the nucleus and binds to TCF1/LEF1 to promote downstream target gene expression."
31060551
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"Cell 119, 641–651 Behrens, J., von Kries, J. P., Kuhl, M., Bruhn, L., Wedlich, D., Grosschedl, R. and Birchmeier, W. (1996) Functional interaction of beta-catenin with the transcription factor LEF-1."
18823282
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"MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
17113388
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"MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
17113388
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"Nuclear beta-catenin and LEF1 were significantly associated with CTNNB1 mutation, showing nuclear beta-catenin a better specificity and positive predictive value for CTNNB1 mutation."
34420090
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"Wnt signaling induces the stabilization of cytosolic beta-catenin, which then associates with lymphoid enhancer factor and T-cell factor (LEF-1/TCF) to form a transcription complex that activates Wnt target genes."
12556497
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"In gastric cancer, MTDH forms a complex with β-Catenin and LEF1 , facilitating the promotion of β-catenin protein translocation and activation of genes downstream of Wnt signaling[ xref ]."
38516242
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"Given the ability of DeltaNDeltaCbetacatenin to act in a dominant negative fashion in interfering with the activity of functional Lef1 and betacatenin complexes in keratinocytes in vitro, we then turned towards using this transgene to assess the consequences of diminished Wnt signaling on hair organogenesis and postnatal cycling."
12135986
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"These structures were accompanied by aberrant TOPGAL activation, reflective of functional Lef1 and beta-catenin complexes (XREF_FIG C)."
12135986
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"Interestingly, in mouse, the related protein HMGB2 binds the Wnt effector transcription factor LEF1 and potentiates transcriptional activation of the LEF1-β-CATENIN complex xref ."
36670152
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"In nuclei, β-catenin associates with the T-cell factor (TCF) and lymphoid enhancer factor (LEF) to induce transcription of target genes xref ."
25170755
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"When LEF1 binds to beta-catenin, it activates epithelial-to-mesenchymal transition and cell proliferation [ xref ]."
34420090
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"Recent studies indicate that increased levels of beta-catenin correlate with the downregulation of the Wnt and beta-catenin pathway due to the proteasomal degradation of beta-catenin and the subsequent decrease in cyclin D [XREF_BIBR], as the cyclin D1 gene might be a target of the beta-catenin and LEF -1 complex [XREF_BIBR]."
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"Β-catenin then forms an active complex with lymphoid enhancer factor and T-cell factor, leading to the transcription of target genes ( xref ). xref , xref "
39617530
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"14 Wnt stabilizes cytosolic beta-catenin, which then binds to TCF and LEF1 in the nucleus and recruits transcription factors Brg1 and CREB binding protein to initiate Wnt targeted gene expression."
28277542
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"Similarly, other studies reported that several lysine acetylation sites of β-catenin, such as K49 and K345, are vital for the β-catenin stability and the interaction of β-catenin and TCFs/LEF1 [ 22 , [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37591442
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"Wnt signaling transiently stabilizes the β-catenin coactivator, which associates with nuclear LEF-1/TCF DNA-binding proteins to activate downstream target genes (Clevers and Nusse 2012)."
29269485
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"During hair follicle development in the mouse, epithelial cells of the ectoderm secrete Wnt3a, leading to nuclear accumulation of beta-Catenin, and mesenchymal cells secrete Noggin, which activates the Lef1 transcription factor; Lef1 can subsequently form a heterodimer with beta-Catenin to regulate transcription of target genes XREF_BIBR."
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"In this scenario, tumor cells with activated EpCAM i.e. the released EpICD associates with FHL2, β-catenin which form a nuclear complex with Lef-1 and induces transcription of genes associated with cell proliferation. (Figure xref ; Schematic diagram modified from Maetzel D et al., 2009) [ xref ]."
28903370
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"Nuclear CTNNB1 binds the transcription factors LEF1 (lymphoid enhancer binding factor 1) and TCF (T-cell factor), thereby converting LEF1 into a transcriptional activator."
23442321
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"The transient or stable expression of Wnt1 could induce the formation of β-catenin–LEF1 complex and the persistent activation of canonical Wnt signaling in CRC cells ( xref )."
32923386
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"As a co-transactivator, β-catenin associates with the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family of transcription factors and mediates expression of target genes involved in differentiation, polarization and cell fate xref ."
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"For example, alterations in the β-catenin-LEF1 and YAP1 signaling pathways have been implicated in melanoma resistance to MAPKi, resulting in reduced apoptosis and enhanced resistance xref ."
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"The thermodynamics of beta-catenin binding to E-cadherin, Lef-1, APC, axin, and the transcriptional inhibitor ICAT have been determined by isothermal titration calorimetry."
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"Namely, the promoters for c-myc and cyclin D1 contain LEF1 consensus sequences that allow β-catenin-LEF1 to bind and modulate c-myc and cyclin D1 expression."
38003292
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"As shown in XREF_FIG, a clear less associated LEF1 was observed in beta-catenin precipitates from DACT2 expressing cells, indicating that the interaction between LEF1 and beta-catenin was evidently impaired in the presence of DACT2."
25023701
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"Then, a downstream β-catenin signaling is initiated, the increasing cytoplasmic β-catenin is translocated to the nucleus and interacts with T-cell factor or lymphoid enhancing factor, resulting in RNA transcription and elongation consequently."
34860240
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"Since APC and the HMG transcription factor Lef-1 both bind to the armadillo repeats of β-catenin ( xref ; xref ), β-catenin must dissociate from APC and subsequently bind to Lef-1 to allow translocation to the nucleus and transcriptional activation."
9362521
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"Additionally, inhibiting the binding of LEF1 to beta-catenin impaired AML growth, in contrast to spared normal hematopoietic stem cells [XREF_BIBR]."
32872365
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"Approaches impairing binding of LEF1 to beta-catenin significantly impaired AML growth, but spared normal hematopoietic stem cells."
31758089
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"This report now demonstrates a striking difference of LEF1 isoform expression between normal and AML cells, contributing to higher vulnerability of leukemic cells to approaches targeting beta-catenin and LEF1 interaction."
31758089
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"In this report, we present evidence that canonical Wnt and beta-catenin signaling is not required for initial activation of Pitx2, but it is required for maintenance of its continued expression likely via direct binding of Lef-1 and beta-catenin to the Pitx2 promoter."
20960542
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"Upon translocation to the nucleus, beta-catenin interacts with T-cell factor 4 (TCF4) and lymphoid enhancer binding factor 1 (LEF1) to regulate the expression of a wide range of genes at the transcriptional level 12."
28811522
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"In the nucleus, β-Catenin binds to T cell factor lymphoid enhancer factor family to activate downstream genes, such as cyclin D1 and C-myc, leading to inhibition of the expression of PPARγ and CCAAT/enhancer binding protein-α (C/EBPα) ( xref ; xref ; xref ; xref )."
36188620
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"As a result, there is cytoplasmic accumulation and nuclear translocation of beta-catenin, where it binds to LEF-1 and TCF, which in turn results in fibronectin induction."
21133803
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"It is possible that α-catenin could abrogate monomeric β-catenin's association with Lef-1 or alternatively stabilize β-catenin's association in the APC complex."
9362521
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"To directly assay for binding of LEF-1 and beta-catenin to these sites, electrophoretic gel-mobility shift analysis was performed with recombinant LEF-1 and beta-catenin produced in E. coli."
10704849
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"These experiments show that there is specific binding of the beta-catenin and LEF -1 complex to either or both of the two TCF recognition sites in the Brachyury promoter."
10704849
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"In human osteoblasts, a naturally occurring LEF1 isoform lacking the N-terminus can still associate with beta-catenin and activate Wnt targets (Hoeppner et al."
22305157
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"This result suggests that beta-catenin loses its ability to translocate into the nucleus and bind to LEF-1 and TCF-4 following TQ treatment."
28275297
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"In the nucleus, β-catenin associates with the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors, converting them from transcription repressors to activators, thereby activating a wide range of Wnt responsive genes xref – xref ."
31273221
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"Elevated beta-catenin levels in colorectal cancer caused by mutations in beta-catenin or by the adenomatous polyposis coli molecule, which regulates beta-catenin degradation, result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of mostly unknown target genes."
10318916
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"However, our results also demonstrate that a complete loss of E-cadherin and transformation to the mesenchymal phenotype are dependent on Smad signaling, which subsequently stimulates formation of beta-catenin and LEF -1 complexes that induce EMT."
16467384
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No evidence text available
15684397
hprd
No evidence text available
8757136
hprd
No evidence text available
12556497
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"We observed that association of β-catenin with the LEF-1 promoter was diminished upon FoxM1 depletion ( xref )."
22014570
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"Thus, the decreased association of β-catenin with the LEF-1 promoter upon FoxM1 depletion was probably because both the nuclear β-catenin level ( xref ) and the binding between β-catenin and TCF4 were decreased ( xref )."
22014570
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"Nuclear RAC1 in complex with β-catenin facilitates RAC1-dependent phosphorylation of β-catenin on serines 161 and 605, resulting in the formation of nuclear β-catenin-LEF-1, which is necessary for the transactivation of Wnt-dependent genes [ xref ]."
35013128
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"For instance, the CTNNB1 gene mutation can result in 90% WNT-activated medulloblastoma, which can induce alterations in the amino acid residues at the phosphorylation site of β-catenin, and LEF1 can form a transcriptional complex with β-catenin in the nucleus."
39881334
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"Cross-talk with the Wnt pathway is possible at the level of β-catenin and Lef-1 interactions with EpICD, and known for induction of the EPCAM promoter by Tcf4."
21552419
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"OSTM1 acts also as an E3 ubiquitin ligase for the heterotrimeric G protein Galphai3 and potentiates WNT canonical signaling by modulating beta-catenin and Lef1 interaction."
30837952
bel
"MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
17113388
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"LEF-1 and TCF proteins associate with beta-catenin through amino-terminal sequences, and together these proteins mediate a transcriptional response to Wnt signaling.Wnt proteins represent a growing fa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10933391
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"Stimulation of mutated Wnt and beta-catenin pathway is well recognized as an early stage in colon carcinogenesis that eventually leads to transcription of beta-catenin and LEF -1 complex such as c-Myc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26482805
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"Transcription factor LEF-1, which acts as a dimer, binds beta-catenin and is involved in signal transduction by the wnt pathway."
11440173
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"The nuclear β-catenin could then specifically bind to lymphoid enhancer factor-1 (LEF-1) transcription factors to activate FUT8 transcription."
30619732
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"The cytoplasmic accumulation of β-catenin after activation of Wnt signaling, or the release of β-catenin due to the loss of E-cadherin, which allow the interactions between β-catenin and LEF-1, can induce the upregulation of Snail, Slug, Twist and mesenchymal markers such as fibronectin ( xref ; xref )."
31849690
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"The result confirmed the tight association between beta-catenin and LEF-1 in the nucleus, further confirming activation of the Wnt signaling (XREF_FIG)."
22391957
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"It is known that LRP5 acts as Wnt co-receptor to form a complex and induce a cascade of intracellular events that stabilize cytosolic β-catenin, which facilitates its transport to nuclei, binds Lef1/T[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16901655
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"However, on stiffer substrates, β-catenin translocates into the nucleus and binds to Lef-1, which occupies the Transcription Start Site (TSS) of the osteogenic transcription factors RUNX2 and OSX [ xref ]."
39943681
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"In colon cancer cells, beta-catenin and LEF1 complexes drive transcription of the full-length LEF1 isoform that can bind beta-catenin at the expense of a dominant negative isoform."
26509276
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"Ets gene PEA3 cooperates with beta-catenin-Lef-1 and c-Jun in regulation of osteopontin transcription."
18343725
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"Examples include antagonists of the MMP2–α v β 3 [ 49 ], Epo–EpoR [ 50 ], and Lef-1–β-Catenin [ 51 ] protein–protein interactions, identified from a cyclic anhydride-derived library."
15939326
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"Given that SOX4 also enhances the association between β-catenin and LEF1, we speculate that SOX4 and LEF1 provide a platform for the assembly of canonical Wnt transcriptional activation complex.Together, these results established that SOX4 cooperates with LEF1 to facilitate the β-catenin nuclear retention, thereby activating the Wnt signaling pathway and inhibiting preadipocyte determination."
36968079
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"The binding of Nek2B,beta-catenin and LEF-1."
31174562
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"Being expressed in the nucleus, β-catenin could bind with lymphoid enhancing binding factor-1/T-cell factor-4 (LEF-1/TCF-4) xref , xref ."
26078714
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"Nuclear beta-catenin binds to TCF and Lef1 to induce transcriptional activity, which is a hallmark of Wnt and beta-catenin signaling activation."
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"A pilot screening of this natural product library in duplicate identified 27 candidate hits that possibly interrupted β-catenin/FITC-LEF1 interaction by >50% inhibition at 30 μM ( Fig. 5 A)."
32956692
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"They antagonize the Wnt pathway by decreasing the cellular beta-catenin, disrupting the beta-catenin and LEF -1 complexes, and repressing the Wnt target genes [XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR]."
23213540
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"Coimmunoprecipitation showed a selective disruption of beta-catenin and LEF -1 interaction."
23213540
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"As shown in Fig. 2 , CTNNB1 binds LEF1, while both of them can interact with HDAC1 [9] ."
22819921
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"WNT signaling is transferred into the cytosol via an atypical GPCR, Frizzled, and the low-density lipoprotein receptor-related proteins 5/6 (LRP) and GSK3, with further transduction to the cell nucleus via the β-catenin–T-cell factor and lymphoid enhancer factor (TCF/LEF) complex to enhance the expression of various targets, including Myc, LGR5, and cyclin D1."
37367867
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"Protein protein interaction between beta-catenin and Lef-1."
14520463
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"XREF_BIBR, XREF_BIBR TGFbeta mediated PI3K-Akt signaling upregulates the transcription factor Snail and induces phosphorylation and inhibition of GSK-3beta which leads to assembly of LEF-1 and beta-Catenin complexes and upregulation of EMT promoting genes."
19471120
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"The expressed LEF1 then associates with beta-catenin to drive further expression of full-length LEF1 [XREF_BIBR]."
26509276
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"In the presence of WNT signals, LEF1 binds to β-catenin via its N-terminal β-catenin-binding domain and promotes context-dependent target gene transcription including c-MYC and AXIN2 ."
27965462
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"Since these RMS cell lines express the full-length LEF1 isoform of 44 kDa, whereas the truncated isoform lacking the β-catenin binding site (31 kDa and 23 kDa; see [ xref , xref ]) were barely detected (Figure xref ), we conclude that these cell lines express functional LEF1 that can interact with β-catenin."
27965462
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"As expected, treatment with Wnt3A or EGF increased the binding of β-catenin to LEF-1 ( xref ), and depletion of LEF-1 ( xref ) reduced Wnt3A- or EGF-induced luciferase activity ( xref )."
32860047
sparser
"Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin."
8757136
sparser
"In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation."
8757136
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"In these cells, the association of endogenous LEF1 and beta-catenin was induced by stimulation with the wnt-1 proto-oncogene."
9419974
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"These results are in accordance with previous studies showing that when CTNNB1 is bound to LEF1 it induces the transcription of LEF1 dependent genes and disturbs the binding of LEF1 to HDAC1 [9] ."
22819921
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"Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend."
8757136
sparser
"After exclusion of autofluorescent and insoluble compounds, sanguinarine showed a dose-dependent inhibition activity against β-catenin/FITC-LEF1 interaction in second FP screening assay ( Fig. 5 B, Ta[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32956692
biogrid
No evidence text available
15684397
sparser
"The results suggest that a cancer pathway driven by wnt-1, or mutant forms of beta-catenin, may involve the formation of a persistent transcriptionally active complex of beta-catenin and LEF1."
9419974
reach
"LEF-1 and beta-catenin form a complex that regulates promoter activation in concert with PAX-5 in immature B cells (Jin et al., 2002)."
21184762
sparser
"Intracellular signal transduction results in the stabilization and nuclear localization of β-catenin, which forms a complex with T cell transcription factors (TCFs) or lymphoid enhancer binding factor 1 (LEF1) to regulate the transcription of target genes [ xref ]."
| PMC
sparser
"Moreover, RAC1 nuclear accumulation in complex with β-catenin occurs following Wnt or RAC1 activation, resulting in RAC1-dependent phosphorylation of β-catenin on serines 161 and 605, promoting the fo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29723737
sparser
"To directly assay for binding of LEF-1 and β-catenin to these sites, electrophoretic gel-mobility shift analysis was performed with recombinant LEF-1 and β-catenin produced in ()."
10704849
sparser
"Based on these insights, new therapeutic opportunities have been explored by investigators who show that small-molecule WNT-pathway inhibitors, which inhibit the interaction between β-catenin and LEF1, selectively induce cell death in AML cell lines and primary AML blasts [ xref ]."
27571104
sparser
"Based on a fact that sanguinarine significantly blocks β-catenin/FITC-LEF1 interaction in FP screening assay, β-catenin could be a possible binding target of sanguinarine."
32956692
sparser
"And SNAIL affects the EMT process of CRC through various mechanisms such as β-Catenin-LEF1 complexes [ xref ]."
| PMC
sparser
"Elevated levels of DVL3 have also been reported in nonsmall cell lung carcinoma. xref CSNK2A2 (overexpressed 11-fold) is a highly conserved and ubiquitous serine/threonine kinase, over-expressed in many tumors including prostate. xref Cytoplasmic CSNK2A2 opposes the inhibitory role of GSK3B by phosphorylating and activating DVL and β-catenin, while nuclear CSNK2A2 enhances β-catenin:LEF1 transactivation of Wnt target genes. xref Its nuclear localization was previously reported as a poor prognostic indicator in prostate cancer, xref potentially through its promotion of AR-dependent transcription. xref Downstream component of the pathway, transcription factor LEF1 was also significantly over-expressed and has recently shown to promote AR expression and consequently enhance growth and invasion ability of prostate cancer cells in vitro . xref "
22915211
sparser
"Nuclear DACT2 disrupts the interaction between β-catenin and LEF1, and cytoplasmic DACT2 represses β-catenin nuclear transport via restoring E-cadherin–β-catenin complex [ xref ]."
32961708
reach
"Active beta-catenin is known to translocate to the nucleus and interact with LEF1 to upregulate the expression of canonical target genes."
26473529
reach
"Free beta-catenin interacts with the DNA binding proteins Tcf3 and Lef1 to form a bipartite transactivator that stimulates the transcription of immediate gene targets."
11524435
reach
"It has been shown that CK1delta and CK2 phosphorylate Lef-1 [XREF_BIBR] leading to a disruption of interactions between beta-catenin and Lef-1, but not between Lef-1 and the template DNA."
23016862
reach
"Increasing evidence has highlighted the negative effect of SFRP5 as an antagonist of β-catenin.61 62 Moreover, previous findings have also revealed that activation of Wnt/β-catenin signaling results in accumulation of β-catenin, which forms a complex with LEF-1 to upregulate the gene expression of MITF."
34291139
reach
"During a high-throughput screening the approved FDA diuretic ethacrynic acid was found to down-regulate Wnt and beta-catenin signaling by inhibiting the formation of the beta-catenin and Lef -1 complex [XREF_BIBR, XREF_BIBR] in chronic lymphocytic leukemia (CLL) cells, although at a low IC 50."
23016862
reach
"In the final step of this pathway, CTNNB1 binds to the transcription factors TCF/LEF-1 (25) and triggers expression of target genes like c-Myc, cyclin D1 and CDKN1A (26)."
40130164
sparser
"Ectopic overexpression of beta-catenin in 293 cells also induced the assembly of the beta-catenin-LEF-1 complex and activated gene transcription from a LEF-1-dependent promotor."
9419974
sparser
"Moreover, Fig. 3 B depicts the complex of CTNNB1 with LEF1 and CREBBP, supporting the hypothesis [9] that an acytyltranferase is also present in the complex of CTNNB1 with LEF1."
22819921
sparser
"It has been shown that phosphorylated β-catenin can interact with LEF-1 but cannot form a ternary complex with DNA xref and cannot drive TCF reporter activity xref ."
20862283
biogrid
No evidence text available
12077367
sparser
"In particular, the increased Histone Deacetylase 6 (HDAC6) expression induces the activation of the nuclear β-catenin-lymphoid enhancer binding factor 1 (LEF1)/T-cell factor-4 (TCF4) transcriptional complex and, in turn, that of the oncogene c-Myc [ xref ]."
32764471
sparser
"Then, β-catenin binds to LEF-1 to regulate Wnt-like, ERE-independent, gene transcription and also binds to ERs to regulate ERE-dependent gene transcription."
19778547
reach
"Immunoprecipitation was performed in order to detect a physical interaction between beta-catenin, alpha-catenin and Lef-1 (XREF_FIG)."
14520463
sparser
"We showed that AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of Wnt/β-catenin pathway facilitated maintenance of glioma stem-like cells and their self-renewal ( xref )."
34353442
reach
"Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
15557752
reach
"This data implicates a strong association between beta-catenin and LEF-1 within the nuclei of cells, as confirmed by immunoprecipitation assay with the nuclear chromatin fraction of MCF-7 cells."
26844628
sparser
"The significance of this was poorly understood until yeast two hybrid studies revealed that the architectural HMG-box transcription factors LEF-1 and XTCF-3 interact with β-catenin ( Behrens et al., 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10495268
sparser
"These results agree with the currently-accepted model that β-catenin associates with DNA-bound LEF1 and functions as a scaffold protein to recruit additional coactivators, such as CARM1 and p300, each of which makes a unique and specific contribution to the highly complex transcriptional initiation process."
21478268
reach
"Furthermore, mammalian Dact1 inhibits the binding of Lef-1 with beta-catenin and promotes the interaction of Lef-1 with histone deacetylase (HDAC1) a co-repressor XREF_BIBR, XREF_BIBR."
23349981
reach
"LEF1 interacts with beta-catenin and plays critical role in proliferation of RCC by activating downstream target genes [XREF_BIBR, XREF_BIBR]."
27766950
reach
"Also, LEF1 may form complexes with beta-catenin (CTNNB1), T-Cell Factor 4 (TCF7L2), and other proteins (HINT1, RUVBL1) implicated in Wnt / beta-catenin signaling, a crucial developmental pathway [XREF_BIBR - XREF_BIBR]."
28376810
reach
"One possible explanation is that over-inhibition of GSK3 increases the expression of the canonical Wnt pathway effector LEF1, and elevated LEF1 interacts with beta-catenin and leads to activation of lineage specification genes Cdx2 and T [XREF_BIBR] (XREF_FIG)."
25595304
reach
"It is known that nuclear beta-catenin is able to bind TCF and Lef1 and activate transcription XREF_BIBR, XREF_BIBR."
25003522
reach
"Thus, β-catenin must first form a complex with TCF7 or LEF1 for this complex to be able to occupy Wnt-responsive elements [32]."
38041178
sparser
"Transcriptional activation is associated with beta-catenin binding to LEF1 facilitating the displacement of co-repressors proteins in response to Wnt signaling."
38113273
sparser
"The Arm R10-12 sequence facilitated transport even when β-catenin was bound to the Arm-binding partner LEF-1, and its activity was stimulated by phosphorylation at Tyr-654."
22110128
reach
"IF staining confirmed the expression of LEF1, TCF3 and TCF4 in purified SPCs (Fig. 4C–E), and co-IP assays demonstrated the binding of β-catenin to LEF1 and TCF3, but not TCF4 in SPCs (Fig. 4F)."
36050783
sparser
"SNAIL1 is a transcription factor usually expressed during EMT; its expression has been demonstrated to induces EMT in gastric cancer [ xref ] although it may direct the cells towards a low proliferating phenotype trough the β-catenin-LEF1 complex [ xref ]."
31906104
biogrid
No evidence text available
15768032
sparser
"Prompted by these findings, we investigated whether TG2 could affect the interaction of β-catenin with LEF1, inside the nucleus, necessary to induce Wnt genes expression."
33674551
reach
"It is accomplished by transactivating MMP-13 promoter activity through LEF1 and beta-catenin binding to the LEF1 binding site in the 3 ' region of the MMP-13 genomic locus."
33572320
reach
"Beta-catenin and TCF -1 pathway in T cell development and differentiation : The discovery of the association between beta-catenin and LEF-1 set the stage for the realization that beta-catenin is found in the nucleus and has a role as a transcriptional co-regulator."
23114888
sparser
"Interaction between β-catenin and LEF-1 was assessed in vivo in C3H10T1/2 SS18-SSX-V5 and C3H10T1/2 pLIVc cells by proximity ligation assay (PLA), using a polyclonal goat anti-LEF-1 and a mouse anti-β-catenin antibody."
26905812
reach
"However, in-depth study is needed to investigate the mechanisms regulating GSK3beta activity in PDLSCs under inflammatory conditions.Our findings demonstrated that in the inflammatory microenvironment[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23911749
reach
"This correlated with upregulation of LEF1, a nuclear binding partner of beta-Catenin."
31463973
sparser
"In such treated cells, the association of beta-catenin with Lef-1 and the transcription of c-myc, cyclin-D1, CD44 and COX-2 were also reduced."
16080193
sparser
"We show that overexpression of CHIP promoted the activation of Wnt signaling, and enhanced the interaction between LEF1 and β-catenin through heightening the K63-linked polyubiquitin chains attached to LEF1, while the knockdown of CHIP had the opposite effect."
33431799
sparser
"In normal epithelial cells, the inhibitory effect of E-cadherin against tumorigenesis is maintained by sequestering the binding of β-catenin to lymphoid enhancer factor (LEF)/T cell factor (TCF); this process could impede the transcription of genes involved in the proliferation-associated Wnt signaling pathway xref ."
33531990
sparser
"In Wnt signaling pathway, LEF1 interactions with beta-catenin transcriptionally activate Wnt regulated genes."
38113273
sparser
"Overexpression of CHIP increased the activation of Wnt signaling, and promoted β-catenin nucleus accumulation by enhancing the interaction between LEF1 and β-catenin through heightening the K63-linked ubiquitination of LEF1."
33431799
reach
"Further downstream, CK2 is capable of regulating the interaction and transactivation activity of a beta-catenin and LEF -1 complex though phosphorylation of LEF-1 at S42, S61 [XREF_BIBR, XREF_BIBR]."
19387549
reach
"In addition, we found evidence that this effect is mediated by Cdx-1 in cells that give rise to the cervical vertebrae.Previous studies have found that cdx-1 expression is stimulated by Wnt signaling [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11335109
sparser
"Co-immunoprecipitation results indicated that DACT2 strongly associated β-catenin as well as lymphoid enhancer-binding factor 1 (LEF1) and directly disrupted the formation of the β-catenin-LEF1 complex in the nucleus."
25023701
sparser
"By qPCR analysis, RUNX2, ALP, and SPARC, which are associated with osteogenic differentiation, were significantly increased 2.47 ± 0.73-fold ( p  = 0.011), 2.57 ± 0.09-fold ( p  = 0.004), and 2.13 ± 0.60-fold ( p  = 0.018), respectively; LEF1 and β-catenin, which are associated with the Wnt/β-catenin pathway, were increased 1.89 ± 0.45-fold ( p  = 0.012) and 2.22 ± 0.51-fold ( p  = 0.017), respectively (Fig.  xref a)."
31753039
reach
"In agreement with these results, several of the new candidates bind to LEF1, and it is well established that TCF and LEF1 forms a complex with beta-catenin to activate the expression of genes under the canonical Wnt pathway [XREF_BIBR]."
20515496
sparser
"The mutations impaired both LEF1 binding to beta-catenin and transcriptional activation, and are the first tumor-associated mutations that inactivate Wnt signaling."
16565724
reach
"In transgenic mouse models, intact Wnt signaling promotes hair follicle differentiation, whereas inhibition of Wnt signaling through the prevention of Lef1 and beta-catenin interaction leads to sebocyte differentiation."
26548488
sparser
"Additionally, β-catenin interacts with Lef-1 and with Klf4 to activate tert gene transcription."
25262238
sparser
"We can see this demonstrated using synthetic peptide BLBD, which can bind to β-catenin and LEF-1 sites, which results in the decreased formation of breast cancer cells [ xref ]."
| PMC
sparser
"Of these two proteins, LEF-1 bound β-catenin with higher significance than TCF4 in both K562 and HEL nuclei."
30630973
reach
"As a consequence, β-catenin accumulates in the nucleus and binds LEF-1/TCF to up-regulate the expression of Wnt target genes Runx2 and Osx in osteoblasts to promote differentiation and proliferation o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24530448
sparser
"If β-catenin is bound with LEF1, TGFβ may not be able to dissociate the complex ( xref )."
14691138
reach
"103 Similarly, the absence of beta-catenin and LEF1 interaction, via mutations of LEF1 within the N-terminal beta-catenin binding domain, is associated with increased sebaceous cell differentiation and development of sebaceous tumors in humans."
22111711
reach
"Functional interaction of beta-catenin with the transcription factor LEF-1."
22716317
sparser
"β-Catenin is an important partner in LEF1 transcriptional factor activity, and when LEF1 is bound to β-catenin, LEF1 transports it to the nucleus ( xref ; xref )."
14691138
sparser
"Interaction of LEF1 with nuclear β-catenin is known to effect Wnt signaling by stimulating the activity of LEF1/TCF transcription factors ( xref )."
14691138
sparser
"The potential explanation is that DACT2 promotes Dvl degradation in a lysosome-dependent pathway and inhibits LEF1 binding to β-catenin [ xref ]."
22806826
sparser
"Moreover, CD44 activates PI3K//AKT pathway allowing the translocation of SIRT1 to the nucleus and its binding to β-catenin and LEF1 to transcribe C-myc, Cyclin D1, and Survivin."
36505828
sparser
"Furthermore, mammalian Dact1 inhibits the binding of Lef-1 with β-catenin and promotes the interaction of Lef-1 with histone deacetylase (HDAC1) a co-repressor xref , xref ."
23349981
reach
"Transcriptional activation is associated with beta-catenin binding to LEF1 facilitating the displacement of co-repressors proteins in response to Wnt signaling."
38113273
sparser
"In the nucleus, β-catenin binds to the lymphoid enhancer factor/T-cell factor to initiate the transcription of target genes that promote the proliferation, migration, and invasion of cancer cells [ 22[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31395338
sparser
"It was previously shown that Dact2 inhibits Lef-1 interaction with β-catenin, however, Dact2 does not appear to inhibit the interaction of Pitx2 with β-catenin or Lef-1, but modulates their synergistic transcriptional activities."
23349981
reach
"Dact proteins have been shown to also modulate Wnt signaling mediators in a ligand independent fashion : Dact proteins shuttle between the nucleus and cytoplasm, and can block nuclear beta-Catenin function by disrupting beta-Catenin and Lef1 complexes and enhancing Lef1-HDAC interaction [XREF_BIBR]."
25099342
sparser
"PROX1 expression was also upregulated in SW480A cells, but only after LEF1-β-catenin had been strongly overexpressed [40] ."
19563800
sparser
"As shown in Fig. xref , CHIP enhanced the interaction between β-catenin and LEF1, and the interaction was further heightened after Wnt3a treatment, while knockdown of CHIP decreased the interaction (Fig. xref )."
33431799
sparser
"Moreover, overexpression of CHIP T246M mutant couldn’t affect the interaction of β-catenin-LEF1 complex (Fig. xref )."
33431799
sparser
"To illuminate whether the heightened interaction of β-catenin and LEF1 was owing to the function of Wnt3a treatment in Fig. xref , we detected the interaction between β-catenin and LEF1 as shown in Fig. xref ."
33431799
sparser
"Under Wnt3a stimulation, the interaction between β-catenin and LEF1 was enhanced, and the interaction was more strengthened with the presence of CHIP."
33431799
reach
"Because it has been reported that LEF-1 interacts with beta-catenin outside the amino-terminal domain, namely, in the middle part of the molecule, the possible participation of beta-catenin has not been formally ruled out."
29998192
reach
"Despite the finding that transcription factor LEF-1 interacts with beta-catenin to activate their target genes, such as EMT related gene SNAI2 XREF_BIBR, and induces EMT, we found that not only full-length LEF-1 but also LEF-1 lacking the N-terminal beta-catenin-binding domain can induce EMT."
29998192
sparser
"We further found CHIP increased the endogenous interaction between β-catenin and LEF1, while CHIP T246M hardly influenced the interaction (Fig. xref )."
33431799
sparser
"Knockdown of CHIP reduced the endogenous interaction between β-catenin and LEF1 (Fig. xref )."
33431799
reach
"Matrix metalloproteinase (s) mediate (s) NO induced dissociation of beta-catenin from membrane bound E-cadherin and formation of nuclear beta-catenin and LEF -1 complex."
12507936
sparser
"These data suggested that CHIP enhanced the interaction between β-catenin and LEF1, and the interaction was further heightened with Wnt3a treatment."
33431799
sparser
"It was in consistent with the enhanced interaction between β-catenin and LEF1 after Wnt3a treatment."
33431799
sparser
"When Wnt/β-catenin pathway is activated, the free cytosolic β-catenin collects in the nucleus and binds with Lymphoid enhancer-binding Factor and T Cell Factor proteins, and subsequently facilitates the transcription of c-Myc, Cyclin D1, MMPs, and other target genes ( xref , xref )."
35117188
sparser
"Moreover, we verify that CHIP promotes the endogenous interaction between LEF1 and β-catenin, the CHIP (p."
33431799
sparser
"T246M) mutant does not affect the interaction, while knockdown of CHIP decreases the endogenous interaction of LEF1-β-catenin complex."
33431799
sparser
"Taken together, these results indicate that CHIP accelerates β-catenin nucleus accumulation by promoting interaction between LEF1 and β-catenin through heightening the K63-linked ubiquitination of LEF1."
33431799
sparser
"MTDH did not interact with LEF-1 in the absence of β-catenin expression, but LEF-1 did interact with MTDH in the presence of β-catenin expression ( Fig. 5 I), suggesting that MTDH interacted with β-ca[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35609735
reach
"hESCs treated with Wnt3A for 6 hours showed rapid binding of beta-catenin and LEF1 to the LGR5 locus as well, indicating that LGR5 is a direct target of these transcription factors (XREF_FIG D)."
28078320
reach
"However, on stiffer substrates, β-catenin translocates into the nucleus and binds to Lef-1, which occupies the Transcription Start Site (TSS) of the osteogenic transcription factors RUNX2 and OSX [54]."
39943681
reach
"The role of beta-catenin in anoikis is also attributable to the activation of the Wnt pathway, in which beta-catenin interacts with transcription factor lymphoid enhancer binding factor 1 (LEF-1) to regulate gene expression and subsequently inhibit anoikis [XREF_BIBR - XREF_BIBR]."
26587543
sparser
"Other work has shown that c-KIT stabilises β-catenin expression [ xref ] which forms a complex with LEF-1 to induce cyclin D1 expression [ xref ]."
27769165
reach
"It was shown that Smad4, beta catenin and Lef1 form a complex to activate expression of the Wnt target gene twin (Xtwn) [XREF_BIBR]."
19926329
sparser
"LEF1 forms a transcriptional complex with β-catenin and acts as an important downstream effector of the Wnt/β-catenin signaling pathway."
36380336
reach
"Considering that stable β-catenin translocates to nuclei and binds to TCF1/LEF1 for transcription activation of the Wnt signaling pathway, we stained β-catenin with FITC and DNA with DAPI for microscope imaging."
36942321
sparser
"In some cell types, but not in others, activated beta-catenin interacts with members of the lymphoid enhancer-binding factor (LEF)/T-cell factor (TCF) family of transcription factors and induces gene expression."
11750994
reach
"Members of the Lef1 and Tcf family of DNA binding proteins bind beta-catenin and transactivate Wnt target genes XREF_BIBR."
19718027
sparser
"Β-catenin may increase FoxO transcriptional activity and competitively limit β-catenin interaction with members of the lymphoid enhancer factor/T cell factor family [ xref ] and β-catenin also has been demonstrated to be necessary for protection against amyloid toxicity in neuronal cells [ xref ]."
18554157
reach
"As a result, there is cytoplasmic accumulation and nuclear translocation of β-catenin, which binds to LEF-1/TCF and decreases SP-A and SP-D expression( Fig. 7 )."
26472220
sparser
"However, it is also possible that Rac1 stimulates β-catenin nuclear transactivation function by stabilizing the interaction between β-catenin and LEF-1 (summarized in xref A)."
26403202
reach
"Upon entering the nucleus, beta-catenin replaces Groucho/TLE, forms complexes with TCF-1 and LEF-1, and activates the transcription of Wnt target genes."
20026746
reach
"To develop an enzyme linked immunosorbent assay (ELISA)-based high throughput screening (HTS) method for beta-catenin and Lef1 interaction antagonists screening, Escherichia coli Rosetta (DE3) competent cells were transformed with beta-catenin-pET-30a (+) plasmid."
31001956
reach
"The Arm R10-12 sequence facilitated transport even when beta-catenin was bound to the Arm binding partner LEF-1, and its activity was stimulated by phosphorylation at Tyr 654."
22110128
sparser
"This regulation required Rac1-dependent phosphorylation of β-catenin at specific serines, which when mutated (S191A and S605A) reduced β-catenin binding to LEF-1 by up to 50%, as revealed by PLA and immunoprecipitation experiments."
26403202
reach
"Taken together, we have successfully developed a simple, robust and reliable ELISA based HTS method for screening of novel Wnt inhibitors targeting beta-catenin and Lef1 interaction."
31001956
reach
"The interaction between beta-catenin and LEF-1 and TCF transcription factors plays a pivotal role in the Wnt-1 signaling pathway."
9874785
sparser
"Similarly, the transient expression of WT-Rac1 or constitutively active (Q61L)-Rac1 caused a significant increase in nuclear β-catenin–LEF-1 interactions relative to control ( xref B)."
26403202
sparser
"Disassembly of the destruction complex can block β-catenin degradation that results in the translocation of the accumulated β-catenin into the nucleus, where β-catenin binds to the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors and triggers the protooncogene-induced transcription of several target genes, such as c-myc, MDR1, OCT4, and cyclin D1 (refs. xref – xref )."
33723262
sparser
"Importantly, we were able to show by IP that the Rac1 inhibitor reduced formation of ectopic LEF-1–β-catenin complexes both before and after LiCl treatment ( xref C;–)."
26403202
reach
"In this study, HT-29 colon cancer cells with truncated APC have LEF-1 and beta-catenin complexes constitutively occupying a WRE in the cMYC enhancer."
19460168
reach
"Transcriptome analysis using gene sets related to the regulation of nuclear beta-catenin signaling and target gene transcription did not reveal a differential expression of the beta-catenin interaction partners LEF1 or TCF7L2 (TCF-4)."
27428425
reach
"Beta-catenin and LEF1 complexes formed in the absence of KLF4 (XREF_SUPPLEMENTARY), and, interestingly, TCF4 and KLF4 complex formation was unaffected by beta-catenin deletion (XREF_SUPPLEMENTARY)."
28589954
sparser
"These results, when considered together, suggest that active Rac1 can stimulate complex formation between β-catenin and LEF-1 and contributes to the positive effect of Wnt on the assembly of β-catenin–LEF-1 complexes in cells ( xref B,D)."
26403202
sparser
"To gain further insight into the effect of Rac1 on the interaction between β-catenin and LEF-1 we analyzed the consequences of mutating the Rac1-sensitive β-catenin serine residues S191 and S605 on binding of LEF-1."
26403202
sparser
"Indeed it has been shown that HDAC1 is an inhibitory factor of Wnt/β-catenin signaling and forms a complex with LEF1 and β-catenin [32] ."
19166851
reach
"During gastrulation, beta-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 and induce EMT [XREF_BIBR]."
32334405
sparser
"We next used immunoprecipitation assays to measure binding efficacy between myc-tagged forms of β-catenin and LEF-1, using an anti-HA antibody to pull down ectopic HA-LEF-1."
26403202
sparser
"Our findings instead suggest that Rac1 enhances the interaction between β-catenin and LEF-1, providing a plausible new explanation for the role of Rac1 in augmenting transactivation of Wnt target genes."
26403202
sparser
"Once in the nucleus, β-catenin binds to two transcription factors, Lymphoid Enhancer Factor-1 (LEF1) and T-cell factor (TCF), displacing the transcriptional repressor Groucho, thereby allowing for binding to WNT-responsive DNA elements [ xref ]."
37048063
sparser
"We propose that this stimulates binding of β-catenin to LEF-1, leading to enhanced Wnt signaling responses."
26403202
reach
"In vitro studies [42] have demonstrated competition for β-catenin between E-cadherin and APC and also between LEF-1 and APC, such that LEF-1 only binds with the β-catenin that is not forming complexes[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24998198
reach
"In this scenario, β-catenin/TCF-1/TCF-4 complexes bind to and transactivate the LEF-1 promoter, which binds more β-catenin to chromatin, thereby generating a self-amplifying increase in the transcript[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24998198
sparser
"Our model ( xref ) details the proposed steps for Rac1 involvement in canonical Wnt signaling: (1) activation (GTP-loading) of Rac1 reduces its association with the plasma membrane and causes active Rac1–β-catenin complexes to dissociate from the membrane, (2) these active Rac1–β-catenin complexes translocate to the cytoplasm and nucleus, (3) active Rac1 mediates the phosphorylation of β-catenin at S191 and S605 via JNK2 kinase in the cytoplasm and possibly the nucleus, and (4) the newly phosphorylated β-catenin preferentially binds to LEF-1 to boost gene transactivation."
26403202
sparser
"As mentioned above, their second selected target, Lef-1, binds nuclear β-catenin in Wnt signaling (section 3.2)."
23531534
reach
"Beta-catenin forms complexes with Tcf and Lef-1 and functions as a transcriptional activator downstream of the Wnt signaling pathway."
9721853
biogrid
No evidence text available
37371581
sparser
"Chibby and ICAT, two physiological inhibitors of β-catenin–LEF-1 interaction ( xref ; xref ) both bind within this region, whereas LEF-1 binds between S191 and S605 within the arm repeats 3–9 ( xref )."
26403202
sparser
"We speculate that addition of the phosphate group at S605 might disturb interaction between β-catenin and the inhibitors, but retains or enhances β-catenin interaction with LEF-1 and recruitment of transcriptional co-activators such as BCL9, pygopus, Mediator and CBP ( xref )."
26403202
sparser
"It has been demonstrated that LEF-1 is constitutively associated with β-catenin in SW480 cells xref ."
20011538
sparser
"The development of small molecule inhibitors targeting β-catenin–LEF-1 interactions have been met with specificity difficulties due to an overlapping binding region between LEF-1/TCFs and destruction complex members such as APC and Axin ( xref )."
26403202
sparser
"Thus further understanding of the mechanism by which Rac1 modulates the interaction between LEF-1 and β-catenin may provide an alternative therapeutic avenue to reduce β-catenin–LEF-1 signaling in colorectal cancer."
26403202
reach
"Treatment with lithium chloride, microinjection of constitutive beta-catenin, HDAC1 IgG, or the addition of serum all acted to reverse this repressive function.Immunoprecipitation from cells transfect[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12464179
reach
"Lef1 can interact with beta-catenin in a transcriptional complex downstream of Wnt signaling XREF_BIBR, and this pathway antagonizes aspects of testis development XREF_BIBR, XREF_BIBR."
22438826
reach
"After nuclear translocation, beta-catenin binds to DNA binding proteins Tcf and Lef1 which subsequently regulates target genes including Cyclin D1 [XREF_BIBR] important in proliferation."
27750091
reach
"Mechanistically, armed with serine/threonine-specific protein phosphatase activity, EYA4 reduced nuclear translocation of beta-catenin by dephosphorylating beta-catenin at Ser552, thereby suppressing the transcription of MYCBP which was induced by beta-catenin/LEF1 binding to the promoter of MYCBP."
31385398
reach
"However, clinical targeted treatment of the beta-catenin and LEF -1 complex remains relatively ineffective."
26750754
sparser
"In vivo, MuSCs underwent a switch from β-catenin-Lef1 to Smad3-Lef1 interactions during the postnatal switch from proliferation to quiescence, with β-catenin-Lef1 interactions recurring during damage-induced reactivation."
30042129
sparser
"Nuclear β-catenin associates with the Lymphoid Enhancer Factor/T-Cell-Specific Transcription Factor (LEF/TCF) family of transcription factors and activates transcription of Wnt target genes (Reviewed in [ xref ])."
21416313
reach
"Studies have shown that the transcription factor Lef1 can transfer into the nucleus and bind with β-catenin to regulate downstream gene expression [42]."
36611960
reach
"Accumulated β-catenin in the cytoplasm translocates to the nucleus and forms a complex with the transcription factor T cell factor (TCF) or lymphoid enhancer-binding factor 1 (LEF1), replacing Groucho and CtBP corepressors [8,19,20]."
37190019
reach
"Thus, we attempted to synthesize a peptide (TAT-NLS-BLBD-6) that could interfere with the interaction of beta-catenin and LEF-1 at nuclei in human breast cancer cells."
26750754
reach
"Since APC and the HMG transcription factor Lef-1 both bind to the armadillo repeats of beta-catenin, beta-catenin must dissociate from APC and subsequently bind to Lef-1 to allow translocation to the nucleus and transcriptional activation."
9362521
sparser
"Proteolysis of EpCAM leads EpICD to form complex withFHL2, β-catenin and Lef1."
26176230
reach
"Combined with the established role of the Wnt signaling pathway in adult hippocampal neurogenesis together with the known association between Lef1 and beta-catenin, our results here suggest that Lef1 is an important part of the Wnt controlled regulation of neural precursor function in the adult dentate gyrus."
25565948
sparser
"Consistent with the above data, mutation of LRE2 and LRE3 alone or in combination attenuated transactivation by LEF1-β-catenin in transfection assays in P19 cells ( Fig. 4 D)."
16309666
reach
"While the full-length LEF1, transcribed from upstream promoter, interacts with beta-catenin and regulates Wnt target genes, the shorter isoform is incapable of binding beta-catenin and suppresses the regulation of Wnt targets through beta-catenin."
20843783
reach
"In consequence of the accumulation of β-catenin in the cytosol and its subsequent translocation into the nucleus, β-catenin binds LEF-1 acting as a coactivator ( Boras-Granic and Hamel, 2013 )."
25800977
reach
"Our research revealed a novel interaction between PITX2, Lef-1, and beta-catenin in which the Lef-1 beta-catenin binding domain is dispensable for its interaction with PITX2."
17785445
sparser
"Both, complete LEF1-deficiency and prevention of the β-Catenin-LEF1 interaction impaired the ability of SNAIL1 to elicit expression of an alternative set of Wnt/β-catenin targets, and to promote cancer cell invasion."
31463973
sparser
"It is notable, however, that although LEF1 did not exhibit detectable binding to the LRE1 under our EMSA conditions, this motif did contribute to LEF1-β-catenin mediated transactivation ( Fig. 4 D), s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16309666
reach
"The beta-catenin and LEF -1 complex is a nuclear response transcription factor and plays an important role in the Wnt and wingless signaling pathway XREF_BIBR."
26750754
reach
"Otherwise, upon Wnt binding to the receptor complex, beta-catenin translocates to the nucleus and binds with the LEF-1 and TCF co-transfection factor."
26750754
reach
"In hepatoblastoma (HB), the knockdown of METTL3 notably decreased the expression and stability of CTNNB1 mRNA, which encodes β-catenin that binds to the TCF4/LEF1 transcription factor and activates target genes such as Jun, MYC, and CCND1, resulting in significant inhibition of HB cell proliferation and colony formation [47]."
40136363
sparser
"While co-transfection of a vitamin D receptor expression vector, along with β-catenin and Lef1, into COS-7 cells, did not alter the expression of a Wnt reporter, the cooperative interactions of β-cate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20138991
sparser
"Over-expression of ILK in intestinal epithelial cells resulted in the expression of an invasive phenotype, which is consistent with Wnt pathway events such as loss of E-cadherin expression, and nuclea[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12191615
sparser
"Previous studies clarified that the N-terminal end of lef-1 binds to β-catenin [24,25,28] ."
15936755
sparser
"Nuclear β-catenin binds to and activates T cell factor (TCF) and lymphoid enhancer-binding (LEF) TFs, which directly bind to specific DNA sequences, enhancing the transcription of genes such as Cyclin D, c-Myc, and Axin2 and inhibiting transcription of other genes such as E-cadherin and the tumor suppressor p53 [ xref , xref ]."
33668092
reach
"There, CTNNB1 replaces TLE and forms a complex with TCF/LEF1, promoting transcription of Wnt target genes ( Molenaar et al., 1996; Huber et al., 1996; Behrens et al., 1996 )."
29279097
reach
"Beta-catenin binds TCF-4 or LEF-1 to regulate cognate gene expression."
21744004
sparser
"LEF1 interacts with β-catenin to regulate cell proliferation and differentiation, facilitating the delivery of β-catenin to target cells [ xref , xref ]."
40004133
reach
"Cross-talk with the Wnt pathway is possible at the level of beta-catenin and Lef-1 interactions with EpICD, and known for induction of the EPCAM promoter by Tcf4."
21552419
reach
"Beta-catenin and LEF -1 complexes target motifs at -75 and -15 within the CCND1 promoter [XREF_BIBR]."
27695879
sparser
"It has been proposed by Billin and collaborators [63] that the activation of LEF1-dependent genes by β-catenin in HEK293 cells involves a two step mechanism, the first being attenuation of the enzymat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16226380
sparser
"Wnt/β-catenin itself enhances EMT: adding it to epithelial cell lines stabilized the complex between cytoplasmic β-catenin and lymphoid enhancing factor-1 (LEF-1), which then transports it to the nucleus, leading to EMT; overexpressed LEF-1 dramatically promotes EMT [ xref ]."
38612719
reach
"In the current study, we synthesized a small peptide to block the interaction between beta-catenin and LEF-1."
26750754
biogrid
No evidence text available
12748295
sparser
"This process results in activation of β-catenin-regulated transcription (CRT), which interacts with transcription factors lymphoid enhancer factor (LEF)/T-cell factor (TCF)."
31867287
sparser
"TCF7 and LEF1 are genes that are responsible for the transcription of downstream genes, and T-cell factor and lymphoid enhancer factor can recruit β-catenin into the nucleus and bind with β-catenin."
38161510
sparser
"In the nucleus, β-catenin binds to the lymphoid enhancer factor/T-cell factor to initiate the transcription of target genes that promote the proliferation, migration, and invasion of cancer cells [ xref ]."
32898953
reach
"10 As a complex, Smad2/3-Smad 4 are then able to enter the nucleus of the epithelial cell and increase transcription of LEF-1, a member of the T-cell family of transcription factors that binds with beta-catenin suppressing transcription of epithelial markers such as E-cadherin and promoting transcription of mesenchymal markers such as vimentin and N-cadherin."
22561306
reach
"XREF_BIBR While collectively these measurements reveal that phosphorylated APC competes effectively with LEF and TCF for beta-catenin binding, recent data suggests phosphorylation of LEF-1 by CK2 also enhances LEF-1 binding to beta-catenin."
19928349
reach
"Lef1, a nuclear mediator of Wnt signaling, associates with beta-catenin and activates Wnt responsive genes."
33329029
reach
"APC binds to CK2 and renders it inactive, leaving LEF-1 in an unphosphorylated form and thereby less able to bind beta-catenin."
19928349
reach
"In our current study, we synthesized a small peptide TAT-NLS-BLBD-6 (ATDEMIPF), which is a Wnt and beta-catenin signaling inhibitor and prevents the binding of beta-catenin to LEF-1 at the nuclear region of human breast cancer cells."
26750754
reach
"Beta-catenin then accumulates in the nucleus and binds LEF-1 and TCF to regulate the expression of Wnt target genes."
25311420
sparser
"As β-catenin binds to LEF1 in the nucleus in the Wnt-canonical pathway ( xref ), we performed β-catenin immunostaining."
28693719
sparser
"Another downregulated gene upon miR-194 inhibition, PITX2, interacts with LEF-1 and β-catenin to increase LEF-1 expression, resulting in the activation of downstream target genes ( xref , xref )."
30542715
reach
"As beta-catenin binds to LEF1 in the nucleus in the Wnt-canonical pathway, we performed beta-catenin immunostaining."
28693719
reach
"In the present study, we explored the mechanism (s) through which NO exerts its effect on cytosolic beta-catenin accumulation and nuclear beta-catenin and LEF -1 complex formation."
12507936
sparser
"Behrens, J. et al. (1996) Functional interaction of beta-catenin with the transcription factor LEF-1."
27568239
reach
"The interaction between β-catenin and LEF- 1 is essential for tumorigenesis, offering valuable insights into the complex molecular networks that regulate cancer progression [11–14]."
40307756
reach
"Upon activation by a canonical Wnt signal, the HMG-box protein Lef1/Tcf can form a complex with β-catenin."
21296678
sparser
"HQ blocked nuclear translocation of β-catenin and lymphoid enhancer-binding factor 1 (LEF-1), and importantly, HQ also reduced the interaction of β-catenin and LEF-1 in the nucleus."
28886972
sparser
"Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
15557752
reach
"Furthermore, the degradation of E-cadherin and the release of beta-catenin to cytosol were accompanied by the formation of nuclear beta-catenin and LEF -1 complex, demonstrating the dissociation of beta-catenin from E-cadherin may be responsible for the activation of beta-catenin and LEF -1 transcription complex."
12507936
sparser
"In hESCs, binding of β-catenin:LEF-1 complexes to distal ME gene enhancers induces cohesin-dependent enhancer–promoter looping and transcription initiation ( xref )."
29269485
reach
"These MMP inhibitors also blocked the cytosolic accumulation of beta-catenin and nuclear formation of beta-catenin and LEF -1 complex."
12507936
reach
"Recent implications for beta-catenin signaling in cancer prompted us to examine colon cancer cell lines for the expression of LEF-1, a transcription factor that binds to beta-catenin."
9419974
reach
"In the nucleus, beta-catenin interaction with the transcriptional effectors TCF and LEF1 converts the latter from transcriptional repressors to activators."
24117889
reach
"In summary, our study found that TAT-NLS-BLBD-6, a cell penetrating pentapeptide, blocks the interaction between beta-catenin and LEF-1 and also efficiently attenuates tumorigenesis in vitro and in vivo."
26750754
sparser
"Regarding the putative role of the LEF1 gene in hernia, it has been shown that the LEF1 protein constitutively associates with β-catenin which mediates the action of the anti-mullerian hormone (AMH) [ xref ], itself involved in the gubernaculum swelling reaction that occurs during the first phase of testicular descent [ xref , xref ]."
25886970
reach
"CK1delta and CK2 phosphorylations of LEF1 on S40 in the N-terminal beta-catenin binding domain XREF_BIBR, XREF_BIBR are dispensable for beta-catenin and LEF1 interaction in vitro, 120 and thus their regulatory roles on LEF1 activity remain to be confirmed."
22111711
reach
"Active beta-catenin binds to LEF1 to form transcription complexes to activate target gene expression."
27441654
reach
"The sum effect of MMP inhibitors demonstrated that NO induced activation of MMP may cause the degradation of E-cadherin and the subsequent dissociation of beta-catenin, thereby contributing to the cytosolic accumulation of beta-catenin and nuclear formation of beta-catenin and LEF -1 complex."
12507936
sparser
"Genes such as cyclin D1 and c-myc are activated following the molecular interaction between β-catenin and lymphoid enhancer binding factor (LEF)/T-cell factor in the nucleus (TCF) xref ."
36646875
sparser
"Β-Catenin mediates the Wnt signaling pathway and associates with the lymphoid enhancer factor–T-cell factor (LEF/TCF) to regulate transcription [202,203] ."
18201548
sparser
"Thus, unlike polarized cells, in mesenchymal cells LEF-1 and β-catenin may form complexes within the nucleus."
10629227
sparser
"Lithium favours lymphoid enhancer factor-1/beta-catenin binding to Gip promoter and diminishes ChIP through T cell factor-4 and histone deacetylase 1."
19582394
sparser
"Under conditions of prodifferentiation Wnt+Activin signaling, YAP bound to β-catenin:LEF-1 complexes at ME gene enhancers can dampen transcription levels by interfering with P-TEFb recruitment and enforcing suboptimal transcription elongation of these genes."
29269485
sparser
"Meanwhile, we found that the interaction between LEF1 and β-catenin plays an important role in condensate formation for both LEF1 and β-catenin."
37657935
sparser
"To investigate whether LEF1 can form phase-separated droplets with β-catenin, we purified recombinant mCherry–LEF1 and mEGFP–β-catenin to perform a phase-separation assay in vitro ( xref )."
37657935
reach
"The nuclear beta-catenin interacts with the transcription factor LEF1 to activate target gene expression (Behrens et al., 1996; Hsu et al., 1998)."
16890162
sparser
"We then investigated whether LEF1 and β-catenin can form co-condensates and if their phase behavior is regulated by protein concentration."
37657935
reach
"These results suggest that Tob1a inhibits the interaction between beta-catenin and LEF1 solely by competing with LEF1 for beta-catenin."
16890162
sparser
"These findings suggest that the interaction between LEF1 and β-catenin plays an important role in condensate formation for both proteins."
37657935
sparser
"In addition, the interaction between LEF1 and β-catenin is essential for their co-condensate formation in vivo."
37657935
reach
"This indicates that Snail can positively regulate PD-L1 and inhibit RKIP expressions at the transcriptional level, possibly via the activation of Wnt signaling in order to promote the β-catenin–LEF1 complex for subsequent binding to the PD-L1 promoter."
38786085
biogrid
No evidence text available
19223551
biogrid
No evidence text available
10825188
sparser
"Upon accumulation, β-catenin interacts with members of the lymphoid enhancer factor/T-cell factor (Lef-1/TCF) family to generate a functional transcription factor complex [2,6,7] which in turn induc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20840866
sparser
"Our results clearly demonstrated that endogenous β-catenin and endogenous LEF-1/TCF proteins in mesenchymal FosER cells were able to form a transcriptionally active complex, which could be increased further upon ectopic expression of β-catenin and LEF-1 without additional signals."
10629227
sparser
"This indicates that Snail can positively regulate PD-L1 and inhibit RKIP expressions at the transcriptional level, possibly via the activation of Wnt signaling in order to promote the β-catenin–LEF1 complex for subsequent binding to the PD-L1 promoter."
38786085
sparser
"As a result it has been further shown that β-catenin interacts with the transcription factor LEF1, which is in turn associated with Smad 4, thereby regulating the transcription of TGF-β target molecules xref ."
25036590
reach
"This protein inhibits Wnt and beta-catenin mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of beta-catenin) to bind to beta-catenin."
15245581
reach
"Dapper1, originally identified as a protein binding to Dishevelled, can associate with HDAC1 and enhance the interaction of Lef1 with HDAC1 (at the expense of Lef1 binding to beta-catenin), thus maintaining Lef1 in the repressive state, as previously proposed."
19433865
reach
"The effect of CGP049090 and PFK115-584, two compounds that specifically inhibit the binding between β-catenin and LEF1, was evaluated in Kasumi-1 and HL-60 AML cell lines, primary AML blasts and healthy peripheral blood mononuclear cells as a control."
31703382
reach
"As a result, the levels of β-catenin and Dvl2 increased, after which β-catenin was transferred to the nucleus and bound to Lymphoid enhancer-binding factor 1 (LEF1) receptor to activate the expression of c-Myc and Cyclin D1, which are related to cell proliferation and differentiation, and activated the Wnt/β-catenin signaling pathway, promoting LR in rats after hepatectomy [125]."
40329344
sparser
"A proteolytic fragment of EpCAM containing the intracellular domain was recently shown to form a complex with β-catenin and Lef-1 that translocates to the nucleus and activates the transcription of ge[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22819673
reach
"Moreover, the miR-371-373 cluster is activated by Wnt signaling through the direct binding of beta-catenin and LEF1 to the promoter region and then forms a positive feedback loop with Wnt signaling by[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26255203
sparser
"Our IP results showed that LEF1 bound to β-catenin in MHCC-LM3 and Huh7 cells ( Supplementary Fig. S6A )."
34767927
sparser
"Of note, β-catenin binds to Lef1 and activates Lef1 transcription complex, leading to down-regulation of E -cadherin expression [ xref ]."
33388549
reach
"LMNADelta9 expressing cells showed a 70% reduction in the nuclear levels of Tcf and Lef1 binding to beta-catenin compared to controls, indicating that Delta9 reduced the levels of active Tcf and Lef-beta-catenin."
20833363
sparser
"HMGB2 potentiates the transcriptional activation of the Lef-1-β-catenin complex, promotes chondrocyte survival and may be a target in aging-related cartilage pathology [83] ."
20206140
reach
"Immunoprecipitation was conducted using β-catenin antibodies, and the interaction between β-catenin and ICAT, TCF3, TCF4, and LEF1 was verified by Western blot."
31207322
bel
"Surplus β-catenin then translocates to the nucleus, interacts with the LEF and TCF transcription factors, and persistently transactivates genes associated with the regulation of cell proliferation, such as MYC and cyclin D1 (Refs 30,32,106,135-137)."
18432252
sparser
"Nurr1 was reported to interact with β-catenin and Lef-1 in 293F cells [36] ."
21362399
sparser
"Further analysis revealed that SMAR1 overexpression led to Wnt3a dependent TCF4/LEF1 transcriptional inhibition by reducing the association of β-catenin and LEF1 ( xref )."
29765542
sparser
"Here, we found that LEF1 interacts with the KDM4A/N-CoR transcriptional repression complex in a β-catenin-independent manner."
37553362
sparser
"Then, β-catenin interacts with T-cell factor (TCF) and lymphoid enhancer-binding factor (LEF) family of transcriptional factors to activate the transcription of target genes [19–21] ."
37591442
biogrid
No evidence text available
15728254
reach
"cAMP response element-binding protein (CREB)-binding protein (CBP) is a co-activator which enhances the binding of β-catenin and TCF/LEF-1 [24]."
36466073
sparser
"Wnt signaling activity is closely associated with glucose level in tumor cells, such that glucose can promote the formation of the lymphoid enhancer binding factor 1 (LEF1)/β-catenin complex, leading to β-catenin acetylation and increased transcriptional activity ( xref )."
35707761
bel
"Surplus β-catenin then translocates to the nucleus, interacts with the LEF and TCF transcription factors, and persistently transactivates genes associated with the regulation of cell proliferation, such as MYC and cyclin D1 (Refs 30,32,106,135-137)."
18432252
sparser
"From the results, we summarise that β-catenin binds to LEF1 to stimulate the transcription of Nup93, which is an immediate downstream target of LEF1."
34767927
sparser
"Behrans J, van Kries JP, Kuehl M, Bruhn L, Wedlich D, o Grosschedl R, Birchmeier W: Functional interaction of beta- catenin with the transcription factor LEF-1."
9330865
biogrid
No evidence text available
27684187
sparser
"Interestingly, both the N- and C-terminal domains of TIEG1 were shown to interact with Lef1 and β-catenin using Duolink assays (Figure xref )."
28201653
sparser
"In many systems, Lef1 interaction with β-catenin is necessary for Lef1-mediated activation of target genes (reviewed in Behrens et al. 1996; Clevers and van de Wetering, 1997 )."
18280717
sparser
"Furthermore, while the Wnt pathway does not initiate activation, the signaling components, β-catenin, and Lef1 , bind to the Pitx2 promotor and are subsequently required for maintaining Pitx2 expression."
33182738
reach
"In the nucleus, beta-catenin directly interacts with LEF-1, activates the keratin gene, promotes hair growth and regulates the HF cycle."
33393222
reach
"Snail acts on the canonical pathway through the upregulation of LEF1, a nuclear binding partner of β-catenin [33]."
40227590
reach
"Mammalian Dpr1 and Dact inhibits expression of Wnt responsive reporters through its promotion of Dvl degradation in a lysosome dependent pathway, and by inhibiting the binding of LEF1 with beta-catenin, but promoting the binding of LEF1 with a corepressor, histone deacetylase 1 (HDAC1) XREF_BIBR, XREF_BIBR."
19440376
sparser
"Epistasis analysis suggested that TRABID might act downstream of beta-catenin stabilization and affect the interaction of beta-catenin with LEF1 [ xref ]."
22584113
reach
"The interaction between beta-catenin, lymphoid enhancer-binding factor 1 (LEF1), and transcription factor 4 (TCF4) was studied in order to see its effects on melanoma."
31934531
reach
"It was observed that the increase in TCF4 and decrease in LEF1 was associated with an invasive transformation of melanoma, in contrast with epithelial tumors where the upregulation of beta-catenin interaction factor LEF1 was seen to promote EMT [89, 90]."
31934531
sparser
"Nuclear β-catenin interacts with members of the T-cell (TCF) or lymphoid enhancer-binding factor (LEF) family and activates Wnt target genes such as the oncogenes cyclin D1 and c-MYC, which ultimately[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26233797
reach
"Moreover, PKF115-584 (that disrupts the interactions between β-catenin and LEF1, leading to transcriptional inactivation of LEF1) prevented and partially reverted leukemogenesis, by inducing apoptosis and reducing proliferation in human T-ALL cells [81]."
32046053
reach
"On the other hand, interactions between Runx2 and beta-catenin and Lef1 inhibit Runx2 mediated activation of osteocalcin [XREF_BIBR], and Runx3 was recently shown to attenuate tumorogenic beta-catenin signaling via direct physical interactions with both beta-catenin and TCF4 [XREF_BIBR]."
19330799
sparser
"According to a previous study, Dact2 promotes disassembly of lysosome-dependent pathway components and suppresses LEF1 binding to beta-catenin [ 10 ]."
31983425
sparser
"A recent study on colon cancer showed that Dact2 can shuttle into and out of the nuclei, with its predominant steady-state location in the cytoplasm dependent on its nuclear export signal; Dact2 direc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31983425
sparser
"Dact2 promotes Dishevelled degradation in the lysosome-dependent pathway and inhibits LEF1 binding to beta-catenin; re-expression of Dact2 stimulates transcriptional activation of T-cell factor-4 and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31983425
reach
"Quantitative analysis indicated that SPDEF disrupted the binding between beta-catenin and TCF1 or TCF3, but did not affect the binding between beta-catenin and LEF1 or TCF4."
28390865
reach
"Chibby, a newly identified β-catenin antagonist, can disrupt the association between β-catenin and LEF1, and promotes β-catenin nuclear export via a chibby-14-3-3-β-catenin complex [81,82]."
32961708
sparser
"Lymphoid enhancer-binding factor 1 (Lef-1) and β-catenin bind the Pitx2 gene promotor in mice, indicating a role for the canonical Wnt signaling pathway in regulating Pitx2 expression."
33425902
sparser
"TIEG1 [ xref ] regulates bone Wnt signaling, affecting β-catenin nuclear localization and interacting with Lef1 and β-catenin."
39371752
sparser
"Β-catenin may increase FoxO transcriptional activity and competitively limit β-catenin interaction with members of the lymphoid enhancer factor/T cell factor family. xref This may lead to cell injury, since β-catenin has been demonstrated to be necessary for protection against Aβ toxicity in neuronal cells. xref "
20716915
reach
"In nucleus, β-catenin binds to LEF-1 and TCF-1 and activates expression of effector target genes in embryonic stem cells ( Fig. 4 )."
28427511
sparser
"Immunoprecipitation (IP) analyses showed that protein-binding of LEF1 to β-catenin ( Fig. 3 C; Fig. S3 A) and to SMAD4 ( Fig. 3 D; Fig. S3 B) were increased after the induction of the osteogenic diffe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25530217
reach
"Nuclear DACT2 disrupts the interaction between β-catenin and LEF1, and cytoplasmic DACT2 represses β-catenin nuclear transport via restoring E-cadherin–β-catenin complex [80]."
32961708
sparser
"Nuclear β-catenin associates with the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors to induce expression of genes involved in cell proliferation and survival, as well as migration and angiogenesis ( xref , xref )."
25228654
reach
"TCF1 and LEF1 can bind beta-catenin and activate target genes when the Wnt pathway is active."
24936456
sparser
"Association of LEF-1 with β-catenin relieves this inhibition and facilitates LEF-1 interaction with chromatin ."
12067647
sparser
"In the activated Wnt signaling pathway, LEF1 forms dimers with over-aggregated β-catenin."
36199361
sparser
"In melanocytes, β-catenin can interact with LEF1 to synergistically regulate the MITF expression (Takeda et al. xref )."
39219677
sparser
"This allows the direct interaction of β-catenin with the lymphoid enhancer factor-T cell factor family of transcription factors and the promotion of cells into the cell cycle ( xref )."
24765201
sparser
"In the nucleus, β-catenin interacts with T-cell factor and/or Lymphoid enhancer-binding factor (LEF) and activates downstream target genes, including c-Myc, cyclin D1, c-Jun, c-fos, fra-1, and members[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37738904
reach
"Specificity of the small molecules was demonstrated by coimmunoprecipitation experiments for the lef-1 and beta-catenin interaction."
20360943
reach
"Coimmunoprecipitation showed a selective disruption of beta-catenin and lef -1 interaction."
20360943
sparser
"Together, these results indicate that Lef1 and β-catenin are associated with IL-1β-mediated MMP13 upregulation in chondrocytes."
17971297
sparser
"In mouse developing lung, conditional knockout of Wnt downstream β-catenin or overexpression of Wnt antagonist DKK1 disrupts peripheral lung epithelial progenitor cell differentiation, 40,41 whereas o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18258849
sparser
"This TFBS was identified by virtue of its interaction with the beta-catenin-T cell-factor/lymphoid-enhancer-factor complex (Tcf/Lef) [ xref ]."
18485226
reach
"In that study, ionizing radiation-treated cervical cancer and nasopharyngeal carcinoma cells showed inhibition of GSK3β by ionizing radiation-activated AKT, resulting in the accumulation of dephosphor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34022282
reach
"Additionally, beta-catenin interacts with Lef-1 and with Klf4 to activate tert gene transcription."
25262238
sparser
"LEF1 interacts with β-catenin and plays critical role in proliferation of RCC by activating downstream target genes [ xref , xref ]."
27766950
reach
"We observe similar molecular interactions between Lef1 and beta-catenin and vertebrate Hipk2, suggesting a direct and conserved role for Hipk proteins in promoting Wnt signaling."
19088090
sparser
"Β-catenin binds with the lymphoid enhancer-binding factor 1 (LEF1) and synergistically upregulates MITF expression and promotes melanogenesis."
38361944
sparser
"Subsequently, β-catenin translocates to the nucleus and binds the lymphoid enhancer factor/T cell factor transcription factor to specifically induce the expression of TJ proteins [ 360 , 361 ]."
31770579
reach
"The association between β-catenin and LEF-1/TCF in the nucleus eventually results in the activation of the transcription process [24]."
36310084
reach
"This regulation required Rac1 dependent phosphorylation of beta-catenin at specific serines, which when mutated (S191A and S605A) reduced beta-catenin binding to LEF-1 by up to 50%, as revealed by PLA and immunoprecipitation experiments."
26403202
reach
"However, it is also possible that Rac1 stimulates beta-catenin nuclear transactivation function by stabilizing the interaction between beta-catenin and LEF-1 (summarized in XREF_FIG A)."
26403202
reach
"Moreover, treatment with the Rac1 inhibitor NSC23766 resulted in a marked reduction in interactions between beta-catenin and LEF-1 in the nuclei of Wnt treated cells (XREF_FIG B)."
26403202
reach
"When LEF-1 interacts with CTNNB1, it leads to upregulation of LEF-1 in SPN."
30283597
reach
"To determine whether the beta, catenin, LEF1, and TCF complex might directly regulate Cacna1g, Cacna2d2, Kcna6, Kcnh8, Drd3, Gabra3, Glra1, Grid2, and Calb2, we analyzed the in vivo binding of beta-catenin to LEF1 and TCF motifs within the CNSs using a chromatin immunoprecipitation (ChIP) assay with designed primers."
23157480
reach
"These results, when considered together, suggest that active Rac1 can stimulate complex formation between beta-catenin and LEF-1 and contributes to the positive effect of Wnt on the assembly of beta-catenin-LEF-1 complexes in cells (XREF_FIG B, D)."
26403202
reach
"To gain further insight into the effect of Rac1 on the interaction between beta-catenin and LEF-1 we analyzed the consequences of mutating the Rac1 sensitive beta-catenin serine residues S191 and S605 on binding of LEF-1."
26403202
reach
"Our findings instead suggest that Rac1 enhances the interaction between beta-catenin and LEF-1, providing a plausible new explanation for the role of Rac1 in augmenting transactivation of Wnt target genes."
26403202
reach
"We propose that this stimulates binding of beta-catenin to LEF-1, leading to enhanced Wnt signaling responses."
26403202
biogrid
No evidence text available
29374064
reach
"Thus further understanding of the mechanism by which Rac1 modulates the interaction between LEF-1 and beta-catenin may provide an alternative therapeutic avenue to reduce beta-catenin-LEF-1 signaling in colorectal cancer."
26403202
reach
"A high proportion of human sebaceous adenomas have amino acid substitutions in the N-terminus of Lef1, which impair the binding of Lef1 to beta-catenin resulting in the inhibition of beta-catenin-dependent transcription of Wnt target genes [XREF_BIBR]."
22960253
sparser
"We conclude that residue F660 is essential for ICAT anchoring but plays no role in the β-catenin interaction with LEF1."
28273108
sparser
"Activation of the Wnt/β-catenin pathway is characterized by 1) stabilization of cytoplasmic β-catenin after receptor engagement by Wnt ligands; 2) β-catenin nuclear translocation, 3) β-catenin interaction with lymphoid enhancer-binding factor-1/T-cell factor-1 (LEF/TCF) transcription factors; and 4) stimulation of target genes ( xref – xref )."
28838333
sparser
"Previous studies have found that members of the lymphoid enhancer factor-1/T-cell factor (LEF-1/TCF) family interaction with β-catenin and their roles as the nuclear effectors of Wnt signaling [ xref , xref ]."
27835604
sparser
"The bHLH-LZ region of MITF-M is responsible for the physical interaction with LEF-1, and beta-catenin is required for the collaboration between LEF-1 and MITF-M. Importantly, MITF-M could function as a non-DNA-binding co-factor for LEF-1."
12753399
sparser
"Increased free β-catenin translocate into the nucleus and bind to the lymphoid enhancer factor/T cell factor (LEF/TCF) family of transcription factors to activate the transcription of target genes lik[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31770525
reach
"Of these two proteins, LEF-1 bound β-catenin with higher significance than TCF4 in both K562 and HEL nuclei."
30630973
biogrid
No evidence text available
37303493
sparser
"This result indicates that β-catenin and Lef1 associate with chromatin at the Tcf/Lef element within the promoter region."
21689639
sparser
"Intratumoral, peritumoral and Crohn’s-like lymphoid reactions were not significantly associated with nuclear CTNNB1 expression ( P > 0.14)."
29510987
sparser
"Loss of Kindlin-1 induces nuclear translocation of β-catenin-Lef1 in Kindler syndrome (KS) and mouse skin, leading to elevated Wnt/β-catenin signaling and premature anagen onset of Kind1-K5 mouse hair[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29544897
sparser
"ChIP experiments demonstrated that β-catenin and Lef1 bind to the enhancer region of the gene."
21689639
biogrid
No evidence text available
18296023
reach
"Wnt/β-catenin signaling activates when β-catenin forms a complex with LEF1 in the nucleus, starting the expression of Wnt target genes."
39823842
reach
"In addition, Wnt signaling activated target gene expression through the interaction of beta-catenin and a LEF-1 and TCF transcription factor family member [XREF_BIBR, XREF_BIBR]."
26580798
reach
"Dkk1 is thought to act as a soluble inhibitor for Wnt signaling [XREF_BIBR], while transcription factor Lef1 mediates Wnt signaling pathway by binding with its co-activator beta-catenin [XREF_BIBR]."
29121993
sparser
"41 In sharp contrast, smad4, another effector of TGF-β, has been shown to form a complex with β-catenin and LEF-1 and to increase β-catenin/LEF-1 transcriptional activity in a [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11839557
reach
"Dact2 promotes Dishevelled degradation in the lysosome-dependent pathway and inhibits LEF1 binding to beta-catenin; re-expression of Dact2 stimulates transcriptional activation of T-cell factor-4 and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31983425
reach
"Both Wnt signalling and Shh signalling are inappropriately activated in epidermal tumours [ 2–4,39 • ], and it therefore comes as a surprise that human sebaceous gland tumours harbour LEF1 mutations t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16919447
biogrid
No evidence text available
10890911
reach
"Whereas it is recognized that beta-catenin and LEF1 interaction is important in the earlier development of tooth formation, the observed decrease in expression may be associated with the function of Lef1 in the regulation of Enam expression."
22243241
reach
"The coordinated binding of beta-catenin and LEF-1 to enhancers is reminiscent of the cooperative binding of recombinant LEF-1 and beta-catenin proteins on chromatin templates in vitro."
25936800
reach
"Because Wnt3a preferentially induces the full-length form of LEF-1, which includes the N-terminal beta-catenin interaction domain, this mechanism ensures mutually interdependent binding of LEF-1 and beta-catenin to chromatin and enables rapid and synchronous activation of downstream target genes."
25936800
sparser
"Considering the specific expression of Lef1 in the RG cells and not in the IPCs, the induction of β-catenin expression should form heterodimeric activators with Lef1 in the RG cells and lead to downstream target activation and generation of IPCs from the RG cells."
23257686
reach
"EA Directly Interacts with LEF-1 and Destabilizes the LEF-1 and beta-Catenin Complex."
20011538
reach
"The chromatin immunoprecipitation assay assay showed beta-catenin and LEF -1 heterodimer could directly bind to the promoter of Mre11 in vivo."
20956948
reach
"When stimulated with Wnt or genetic mutations of Wnt components, β-catenin accumulates in the cytoplasm, translocates into the nucleus and binds to LEF-1/TCF4 and some other coregulators, leading to the expression of target genes, such as c-Myc and MMP7."
33893396
sparser
"CCAR2 stabilizes the LEF1-β-catenin interaction by inhibiting SIRT1-mediated deacetylation of β-catenin by blocking their interaction, thereby enhancing LEF1-β-catenin complex formation on Wnt responsive enhancers and increasing the expression of Wnt/β-catenin target genes, including cancer-promoting TFs PROX1 and MACC1 xref , xref (Fig. xref )."
37524873
reach
"Similar mutations, leading to an increased half-life of beta-catenin, were identified in several human melanoma cell lines leading to a constitutively active beta-catenin and Lef -1 complex in these c[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10528152
reach
"In the nucleus, β-catenin binds to LEF1/TCF to initiate transcription and expression of target genes, Runx2 and Osterix, thereby regulating the proliferation and differentiation of osteoblasts."
39982061
reach
"To further test the role of Beta-catenin in Lef1 activation, we blocked the interaction between Lef1 and Beta-catenin using the inhibitor iCRT3."
36243826
reach
"In addition, Beta-catenin could bind to and activate Lef1 through the Wnt signalling pathway, and such activation could be facilitated by cadherin junctions ."
36243826
reach
"Β-catenin binds with the lymphoid enhancer-binding factor 1 (LEF1) and synergistically upregulates MITF expression and promotes melanogenesis."
38361944
reach
"E-cadherin localized with β-catenin in the nucleus suppresses the LEF-1-mediated activation of the Wnt downstream target genes via recruiting β-catenin and prevents its nuclear localization, leading to inhibiting the interaction between β-catenin and LEF-1 (99)."
34713301
sparser
"Once inside, β-Catenin is associated with the T-cell factor (TCF) and lymphoid enhancer factor (LEF); it functions by regulating multiple cellular mechanisms, thus behaving as a transcriptional switch [ xref ]."
36359888
sparser
"In the presence of Wnt, β-catenin translocate to the nucleus where β-catenin forms an active complex with lymphoid enhancer factor (LEF) and T-cell factor (TCF), thereby activating downstream genes [ [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31178140
sparser
"Β-Catenin-binding to Lef1 converts this complex into a potent transcriptional activator."
20006729
sparser
"ILK has been shown to regulate cell fate via nuclear interaction of beta-catenin with lymphoid enhancer factor (LEF-1) transcription factors."
15673299
reach
"Wnt signaling promotes EC proliferation (Masckauchan et al., 2005, 2006) and the Lef1/Ctnnb1 complex induces cell cycle progression through transcriptional activation of Cyclin D1 (Shtutman et al., 1999)."
19154719
sparser
"In the nucleus, β-catenin forms an active complex with T-cell factor and lymphoid enhancer factor proteins by displacing the TLE/Groucho complexes and recruiting histone modifying co-activators, such as CBP/p300, BRG1, BCL9 and Pygo ( xref )."
34036399
sparser
"Maetzel et al ( xref ) firstly demonstrated that a proteolytic fragment of EpCAM containing EpICD forms a complex with β-catenin and LEF-1, which translocates to the nucleus and activates the transcription of genes associated with cell proliferation, such as c-Myc and cyclins A and E, thereby promoting oncogenesis ( xref ) ( xref )."
30015855
sparser
"The expressed LEF1 then associates with beta-catenin to drive further expression of full-length LEF1 [ xref ]."
26509276
sparser
"The Wnt signal is transferred into the cytosol and thereby further transduced to the cell nucleus via the β-catenin–T-cell factor and lymphoid enhancer factor (TCF/LEF) complex to enhance the expression of targets, including Myc and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)."
36903626
sparser
"In vitro phosphorylation of purified Lef-1 was used to examine the effect of CK2 on the interaction of Lef-1 with β-catenin."
21075118
sparser
"A further study showed that the expression of Isl1 was directly regulated by β-catenin, which can interact with Lef-1 and bind to Isl1 promoter."
34238675
reach
"29 These inhibitors and their mechanism of action (inhibition of LEF-1 and beta-catenin binding) are being more fully explored with the development of a LEF-1 and beta-catenin FRET assay."
12659745
reach
"The ESR1 and beta-catenin conjugation then associated with the transcription factors TCF3 and LEF1."
26432349
reach
"Surprisingly, beta-catenin directly interacts with the transcription factor LEF-1 and XTCF-3, and thereby is not only translocated into the nucleus but also modulates the properties of LEF-1 and XTCF-3 as a transcription factor."
9046239
reach
"In pull-down assays, HINT1 did not bind to beta-catenin or LEF-1 nor did it impair the LEF-1 and beta-catenin interaction or the binding of pontin or reptin to beta-catenin."
21316334
sparser
"E-cadherin localized with β-catenin in the nucleus suppresses the LEF-1-mediated activation of the Wnt downstream target genes via recruiting β-catenin and prevents its nuclear localization, leading to inhibiting the interaction between β-catenin and LEF-1 ( xref )."
34713301
sparser
"These results suggested that Pax3 did not affect β-catenin levels but, rather, may interact with Lef1 and β-catenin in a ternary complex."
20006729
sparser
"Hence, an immuno-precipitation assay was performed in RANG-2 cells to analyze interaction of LEF1 with β-catenin."
30530053
sparser
"Expectedly, under conditions of low FBXO16 expression, more β-catenin was bound to LEF1 in contrast to cells that had higher levels of FBXO16 ( xref G )."
30530053
sparser
"Β-catenin then forms the dimeric β-catenin-LEF1 complex that activates the transcription of Wnt target genes [ xref ]."
37147457
sparser
"After nuclear translocation, β-catenin interacts with Lef-1 and binds to Isl1 promoter together, which promotes the transcription of Isl1 [87] ."
34238675
reach
"Our IP results showed that LEF1 bound to β-catenin in MHCC-LM3 and Huh7 cells ( Supplementary Fig. S6A )."
34767927
reach
"XREF_BIBR - XREF_BIBR Although it is well established that the formation of nuclear beta-catenin and LEF1 complexes has a crucial role in the activation of Wnt target genes, the details of the regulatory mechanisms of beta-catenin activity and its interaction with LEF1 are still under investigation."
26477307
reach
"From the results, we summarise that β-catenin binds to LEF1 to stimulate the transcription of Nup93, which is an immediate downstream target of LEF1."
34767927
reach
"The accumulated β-catenin in the nucleus subsequently activates Nup93 transcription by binding to LEF1, which ultimately facilitates the Nup93-dependent HCC metastasis and progression.Several studies [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34767927
reach
"OTUD7B Promotes the Interaction between LEF1 and beta-Catenin."
37371581
sparser
"Isothermal titration calorimetry revealed that β-catenin binds to non-phosphorylated or CK2 phosphorylated Lef-1 with the same affinity, which is consistent with the absence of phosphoSer40 interactions in the crystal structure of phosphorylated Lef-1 N-terminal domain bound to β-catenin."
21075118
sparser
"Β-Catenin in the nucleus interacts with transcription factor T-cell factor (TCF) and/or lymphoid enhancer factor (LEF), promoting expression of specific genes."
26109834
sparser
"Moreover, PKF115-584 (that disrupts the interactions between β-catenin and LEF1, leading to transcriptional inactivation of LEF1) prevented and partially reverted leukemogenesis, by inducing apoptosis and reducing proliferation in human T-ALL cells [ xref ]."
32046053
sparser
"During gastrulation, β-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 and induce EMT [ xref ]."
32334405
reach
"We further investigated whether the increased nuclear translocation of LEF1 by OTUD7B leads to enhanced transcriptional activity by promoting the interaction between LEF1 and β-catenin."
37371581
reach
"This clearly demonstrates that OTUD7B promotes the nuclear localization of LEF1 and enhances the interaction between LEF1 and β-catenin in the nucleus, resulting in enhanced transcriptional activation."
37371581
sparser
"Although β-catenin–mediated attenuation of HDAC1 activity is important for the transcriptional activity of β-catenin-LEF1 complex, how β-catenin enzymatically inactivates HDAC1 remains unclear."
37147457
sparser
"As shown in xref , β-catenin binds to LEF-1 to inhibit CDH1 transcription and cause EMT during gastrulation [ xref ]."
39959483
sparser
"However, it has also been reported that the inhibition of interaction between β-catenin and Lef-1 caused HFSCs to differentiate into sebocytes xref , xref ."
25076848
reach
"Functional interaction of beta-catenin with the transcription factor LEF-1."
8757136
sparser
"This differential expression of the LEF isoform in LSC versus normal HSCs offers the opportunity to preferentially target LSCs by impairing LEF1-β-catenin interaction, and targeting Wnt/β-catenin axis."
32046053
reach
"Some studies reported promising results in APC mutated CRC cell lines [XREF_BIBR, XREF_BIBR], whilst others showed that nuclear beta-catenin bound to LEF-1 and TCF transcription factors would be protected from degradation such that TNKSi treatment would not be effective [XREF_BIBR]."
26930278
sparser
"These data indicate that the effect of CK2 on the Wnt/β-catenin pathway does not appear to be at the level of the Lef-1–β-catenin interaction."
21075118
reach
"Interaction between beta-catenin and LEF-1 was assessed in vivo in C3H10T1/2 SS18-SSX-V5 and C3H10T1/2 pLIVc cells by proximity ligation assay (PLA), using a polyclonal goat anti-LEF-1 and a mouse anti-beta-catenin antibody."
26905812
reach
"Recent biochemical studies indicate that the vertebrate HMG-domain proteins Lef-1 and XTcf-3 can physically interact with beta-catenin, a homologue of Drosophila Armadillo (Arm), the most downstream component known in the Wnt signal transduction pathway."
9039917
reach
"According to a previous study, Dact2 promotes disassembly of lysosome-dependent pathway components and suppresses LEF1 binding to beta-catenin [ 10 ]."
31983425
reach
"However, when the Wnt pathway is activated, β-catenin accumulates in the nucleus and forms a complex with LEF1."
38003292
reach
"Calcium ions can modulate the activity of Wnt signaling components, including LEF1, by affecting β-catenin stability, the interaction between β-catenin and LEF1, or downstream signaling events."
38003292
reach
"Upon the binding of HIF1α protein to HRE or the binding of β-catenin to TCF/LEF1, the luciferase reporter gene is transcriptionally activated."
36490351
trips
"Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
15557752
sparser
"The interactions between β-catenin and LEF1 have been historically viewed as inseparable."
32824435
reach
"Furthermore, we demonstrate that TGFbeta dependent activation of LEF1 and TCF target genes can occur in the absence of beta-catenin binding to LEF1 and TCF and requires both Smad and LEF1 and TCF DNA binding sites in the Xtwn promoter."
10890911
sparser
"Pitx2 can also be an effector of this pathway, for example, in epithelial cells it forms a functional complex with β-catenin and Lef1 to synergistically induce transcription from the Lef1 promoter ( A[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19850024
sparser
"The dimer of Lef1 and β-catenin regulate the expression of downstream transcription factors after the Wnt signaling pathway is activated, playing an important part in myogenesis and adipogenesis [ xref ]."
36611960
reach
"Furthermore, endogenous interaction between beta-catenin and LEF1 was decreased in DBC1 KO SW480 cells (XREF_FIG), suggesting that DBC1 stabilizes the interaction between beta-catenin and LEF1 by interacting with beta-catenin."
26477307
sparser
"LEF-1 transcription factors are regarded to be the most downstream effectors in the wnt-signalling regulation pathway, forming a complex with β-catenin before binding DNA."
10433970
reach
"Thus, expression of the OSTM1DeltaC mutant blocked the ability of Wnt3a to signal to a major embryonic response, PE formation.The epistasis experiments implicated OSTM1DeltaC action on Wnt and beta-ca[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18296023
reach
"We compared the ability of OSTM1 and OSTM1DeltaC to regulate the Wnt3a induced binding of beta-catenin to Lef1."
18296023
reach
"Beta-catenin binding to Lef1 increased in cells treated with Wnt3a."
18296023
sparser
"In order to compare the interactions of Tcf family members with β-catenin, we have determined crystal structures of non-phosphorylated and CK2-phosphorylated Lef-1 bound to the armadillo domain of β-catenin and Lef-1."
21075118
sparser
"Coimmunoprecipitation data demonstrated that overexpression of NLK protein reduced β-catenin binding to LEF-1."
24395089
reach
"Furthermore, we determined the mechanism whereby OSTM1 regulates Wnt signaling : through modulation of the beta-catenin and Lef1 interaction, a crucial downstream event in Wnt and beta-catenin signal [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18296023
reach
"Therefore, we tested the ability of OSTM1 and OSTM1DeltaC to regulate the beta-catenin and Lef1 interaction."
18296023
reach
"It will be informative to probe the beta-catenin and Lef1 interaction in cells from grey-lethal mice or ARO patients with OSTM1 deficiency.Finally, expression of OSTM1DeltaC was shown to inhibit the f[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18296023
reach
"Consistent with the model in which beta-catenin acetylation enhances its binding to LEF1 and leads to increased chromatin occupancy of LEF1 by stabilizing LEF1, beta, and catenin complex, XREF_BIBR, XREF_BIBR our results further showed that DBC1 enhances the interaction of beta-catenin with LEF1 (XREF_FIG) and is required for efficient recruitment of LEF1 and beta-catenin to their target chromatin regions (XREF_FIG), probably through protecting beta-catenin from SIRT1 mediated deacetylation (XREF_FIG)."
26477307
sparser
"What is the structural basis of specific protein-protein interactions, such as those between homeodomain proteins and β-catenin-Lef1 interactions, and how are these determinants altered in transcripti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10456033
reach
"Binding of β-catenin to TCF/LEF1 causes the removal of Groucho and HDAC, whereas histone acetylase re-acetylates histones, unpacking DNA and facilitating transcription [23] ."
26851176
reach
"Here, we present ITC measurements of Lef-1 and Tcf4 binding to beta-catenin under identical conditions, which show that Lef-1 binds slightly less strongly than Tcf4 to beta-catenin."
21075118
reach
"Of note, beta-catenin binds to Lef1 and activates Lef1 transcription complex, leading to down-regulation of E-cadherin expression [XREF_BIBR]."
33388549
reach
"Nuclear β-catenin displaces these co-repressors, binds the amino-termini of Lef1/Tcfs, enhances their interactions with chromatin, and recruits co-activators (e.g."
15474285
sparser
"We also find that CK2 phosphorylation of Lef-1 produces no change in affinity or in the structure of the complex, indicating that the effects of CK2 on β-catenin-mediated transcription are not at the level of the Lef-1–β-catenin interaction."
21075118
reach
"LiCl activation of the Wnt and beta-catenin signaling pathway causes nuclear accumulation of beta-catenin, which binds with TCF and LEF1 transcription factor to modulate the expression of downstream g[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29679546
reach
"The extended N-terminal region of Lef-1 binds to beta-catenin using most of the conserved sequence motif Dxthetathetaxphix 2-7 E."
21075118
sparser
"The transient expression of Wnt1 induces the formation of β-catenin–LEF1 complex in nucleus, resulting in persistent activation of canonical Wnt/β-catenin signaling xref ."
36056132
sparser
"The extended N-terminal region of Lef-1 binds to β-catenin using most of the conserved sequence motif Dxθθxφx 2–7 E. There are relatively few direct contacts between Lef-1 residues 11–18 and β-catenin, which include a salt bridge between Asp12 and Arg515, and the backbone carbonyl oxygen of Gly11 forms a hydrogen bond with Arg582 ( xref )."
21075118
reach
"Consequently, we evaluated their interaction in endothelial cells and found that the treatment of HMVECs with YKL-40 induced an association between β-catenin and LEF1 (Figure 3G)."
37686533
reach
"Given the difference in the structures of TCF-4 and Lef-1 bound to beta-catenin, it was of interest to compare the thermodynamics of these complexes."
21075118
reach
"Intracellular signal transduction results in the stabilization and nuclear localization of β-catenin, which forms a complex with T cell transcription factors (TCFs) or lymphoid enhancer binding factor 1 (LEF1) to regulate the transcription of target genes [8]."
| PMC
sparser
"Binding of β-catenin with the transcription factors of the lymphoid enhancer-binding factor (LEF)/T-cell factor (TCF) family switches the inhibitory state of LEF/TCF to the activating state and turn o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20006983
biogrid
No evidence text available
15525529
sparser
"Wnt/β-catenin signaling activates when β-catenin forms a complex with LEF1 in the nucleus, starting the expression of Wnt target genes."
39823842
reach
"TCF1, LEF1 and TCF4 can bind beta-catenin and activate transcription of target genes as a result of Wnt signaling."
29443926
sparser
"Subsequently beta-catenin interacts with members of the lymphoid enhancer factor 1/T-cell factor (LEF1/TCF) family, resulting in a functional transcription factor complex and the expression of downstream target genes. xref , xref The non-canonical Wnt ligands also bind to FZD family receptors, and ROR2 and RYK co-receptors. xref - xref The non-canonical signaling pathways include three pathways (Wnt/Ca 2+ , Wnt/G protein, and Wnt/PCP signaling pathways) and non-canonical signaling regulates cell polarity and movement. xref - xref Among the five Wnt antagonist families (secreted frizzled-related protein (sFRP), Wnt inhibitory factor 1 (Wif1), Xenopus Cerberus, Wise and Dickkopf (DKK) families) the DKK family consists of four main members (DKK1-4) which contain two distinct cysteine-rich domains. xref , xref Our lab has studied several Wnt antagonist genes and these function in renal cancer. xref - xref Previously DKK4 has been thought to act as an inhibitor of Wnt/beta-catenin signaling in colorectal cancer. xref , xref Recently two groups found that DKK4 expression was increased in colon cancer tissues compared to matched normal colon tissues and DKK4 was induced by activated beta-catenin, although DKK4 itself significantly inhibited TCF/LEF reporter activity in colon cancer cell lines. xref , xref As far as we know, there have been no reports regarding DKK4 and renal cancer."
21472712
sparser
"These results showed that β-catenin and LEF1 could form a ternary complex with the consensus sequence in the Anf promoter."
19799869
sparser
"In order to assess the possible effects of CK2 phosphorylation on the Lef-1–β-catenin interaction, the complex of the CK2-phosphorylated Lef-1(1–63) and β-cat-arm was purified and crystallized."
21075118
sparser
"In this smaller lattice, Lef-1 Asp26, Gly28, Pro30, Gln31, Glu33, Phe36, Ala37, and Glu38 interact directly with neighboring β-catenin molecules ( xref ); note that Glu33 forms a salt bridge with Arg647 of a neighbor as well as Arg386 (see above)."
21075118
reach
"These results suggest that accumulated beta-catenin proteins may bind to the lymphocyte enhancer binding factor-1 (LEF-1), form the beta-catenin and LEF -1 complex, and stimulate such promoters regulating the cell cycle as the cyclin D1 gene."
12375019
reach
"In principle this is possible, as it was recently shown that β-catenin–LEF-1 complexes were not retained in the nucleus when the chromatin-binding domain of LEF-1 was mutated ( Sharma et al., 2012 )."
22433990
reach
"[Development of an ELISA based high throughput screening method for novel anticancer agents targeting beta-catenin and Lef1 interaction]."
31001956
sparser
"Approaches impairing binding of LEF1 to β-catenin significantly impaired AML growth, but spared normal hematopoietic stem cells."
31758089
sparser
"Accordingly, ectopic LEF1 expression increases β-catenin binding to TCF-binding sequences and activates the MMP7 promoter [ xref ]."
28757546
sparser
"Residues 11–18 adopt a conformation different from the equivalent portions of other Tcfs, which may be due to differences in lattice packing, as neighboring molecules of β-catenin interact with some of this region of Lef-1. (Interestingly, pLef-1 Cys16 forms a disulfide bond with β-catenin Cys350 from a lattice neighbor, even though its conformation is the same in the non-phosphorylated structure.) It is also possible that some of the difference arises from the insertion of Pro13 between two acids that are otherwise adjacent in sequence in the other Tcfs ( xref ); Lef-1 Asp12 forms a salt bridge with β-catenin Arg515 ( xref ), whereas the equivalent interaction is absent in other TCF–β-catenin structures."
21075118
reach
"Similarly, the interaction between nuclear beta-catenin and TCF-4 or LEF-1 was reduced by CaSR activation, indicating an inhibitory association of beta-catenin and TCF-4 or LEF-1 in the nucleus."
27108064
sparser
"The nuclear β-catenin interacts with the transcription factor LEF1 to activate target gene expression ()."
16890162
sparser
"These results suggest that Tob1a inhibits the interaction between β-catenin and LEF1 solely by competing with LEF1 for β-catenin."
16890162
sparser
"In addition to TCF4 mainly (Gotoh et al. xref ), LEF-1 (Grueneberg et al. xref ), HBP1 (Sampson et al. xref ), and UBF2 (Grueneberg et al. xref ) can also affect the cyclin D1 promoter activity by interaction with β-catenin or LEF-1."
22704330
reach
"In response to Wnt signaling, LEF-1 bound to both DNA and β-catenin activates Wnt-responsive genes."
35177069
sparser
"In consequence of the accumulation of β-catenin in the cytosol and its subsequent translocation into the nucleus, β-catenin binds LEF-1 acting as a coactivator ( Boras-Granic and Hamel, 2013 )."
25800977
reach
"Activation of the Wnt and beta-catenin pathway is characterized by 1) stabilization of cytoplasmic beta-catenin after receptor engagement by Wnt ligands; 2) beta-catenin nuclear translocation, 3) beta-catenin interaction with lymphoid enhancer binding factor-1 and T-cell factor-1 (LEF and TCF) transcription factors; and 4) stimulation of target genes."
28838333
reach
"In the nucleus, CTNNB1 forms a complex with LEF1, inhibits the transcriptional activity of E-cadherin, and induces epithelial-mesenchymal transition [23]."
31511060
biogrid
No evidence text available
16510874
sparser
"On the other hand, changes in the expression of the key molecules needed for active canonical Wnt pathway, like LEF1 (2.0-fold induction) and β-catenin interacting protein (12.5-fold reduction) were o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20435172
sparser
"In the presence of Wnt, the β-catenin transcriptional co-activator translocates to the nucleus and associates with members of the T-cell factor/ lymphoid enhancer factor (TCF/LEF; hereafter, TCF) family of transcription factors [ xref , xref ]."
30794691
reach
"Coimmunoprecipitation data demonstrated that overexpression of NLK protein reduced beta-catenin binding to LEF-1."
24395089
sparser
"According to Du et al. [ xref ], the binding of β-catenin and LEF1 to the CD274 promoter region stimulated transcription."
37496925
sparser
"Nuclear β-catenin binds to LEF1, displacing the Groucho/TLE repressors, resulting in transcriptional activation of downstream target genes (c-MYC, Cyclin D1, and LEF1) in response to activation of canonical WNT signaling [ xref ]."
29535816
sparser
"These results suggest that DBC1 is required for efficient binding of LEF1 to its regulatory regions and/or for stable association of LEF1 with β-catenin, which strongly enhances the binding of LEF1 to chromatin, xref and thereby facilitates the occupancy of p300 and Pol II on the regulatory regions of LEF1–β-catenin target genes."
26477307
reach
"In the Wnt/β-catenin signaling pathway, accumulated β-catenin in melanocytes enters the nucleus and binds to LEF1, thereby acting on the promoter region of MITF and affecting its transcription level [8]."
37048437
reach
"Surprisingly, however, phosphorylation of murine Ser40 residue (corresponding to human Ser42) by CKIdelta disrupts the beta-catenin and LEF -1 complex [XREF_BIBR]."
24594309
reach
"Elevated beta-catenin levels in colorectal cancer caused by mutations in either beta-catenin or in the adenomatous polyposis coli protein, which regulates the degradation of beta-catenin, result in th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11223032
sparser
"Thus, we checked whether β-catenin also binds to the Lef1-binding site."
17945188
reach
"Meanwhile, we found that the interaction between LEF1 and β-catenin plays an important role in condensate formation for both LEF1 and β-catenin."
37657935
sparser
"Mammalian Dpr1/Dact inhibits expression of Wnt-responsive reporters through its promotion of Dvl degradation in a lysosome-dependent pathway, and by inhibiting the binding of LEF1 with β-catenin, but promoting the binding of LEF1 with a corepressor, histone deacetylase 1 (HDAC1) xref , xref ."
19440376
reach
"In addition, the interaction between LEF1 and β-catenin is essential for their co-condensate formation in vivo."
37657935
reach
"These findings suggest that the interaction between LEF1 and β-catenin plays an important role in condensate formation for both proteins.We further explored the phase separation of LEF1 IDR-depleted mutants in vitro."
37657935
sparser
"In this study, it was observed that the overexpression of wild type LEF1, which can interact with β-catenin, decreased the endogenous levels of Notch1 in a dose-dependent manner (left panel of A)."
19000719
signor
"Phosphorylated lrp5/6 leads to inhibition of the so-called beta-catenin destruction complex (which includes axin, gsk3, dvl, ck1, and the tumor suppressor adenomatous polyposis coli), resulting in the stabilization and translocation of beta-catenin in the nucleus, where it activates target genes through binding to tcf/lef transcription factors."
21078818
reach
"Wnt3a stimulation of C3H10T1/2 pLIVc (0.1 muM for 16h) did not result in significant dissociation of HDAC from LEF-1 (not shown) whereas it increased association between LEF-1 and beta-catenin, as discussed earlier (XREF_FIG)."
26905812
reach
"Finally, based on the fact that certain vertebrate TCFs encode either repressor or activator function and that repressive TCF proteins have higher affinities for Gro/TLE, a model has been proposed by which Gro/TLE in complex with repressive TCFs (e.g., TCF3) is released upon Wnt signaling, allowing for the recruitment of activating TCFs (e.g., LEF1) bound to β-catenin [18]."
32759724
reach
"This is in line with the reported three-dimensional (3D) structures of β-catenin bound to LEF-1 and Axin-1 (PDB: 3OUW and PDB: 1QZ7 , respectively; Sun and Weis, 2011 ; Xing et al., 2003 ), showing th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34320339
reach
"Transcriptional activation of Xenopus twin , a gene related to siamois , also requires smad activity, mediated by complex formation between smad4, β-catenin, and Lef1/Tcf ( Nishita et al., 2000 )."
15680369
sparser
"LEF-1 and β-catenin form a ternary complex with DNA that displays an altered DNA bend ( xref )."
19458225
reach
"On the other hand, beta-catenin present in the nucleus binds to Lef1 (k 49) to increase the expression of mRNA of Delta gene (k 54), followed by translation of Delta (k 51) and translocated to the cytoplasm (k 58)."
28106087
reach
"During ADT, when androgen-bound AR is diminished or absent in the nucleus, a complex formation between the transcription factor TCF/LEF-1 and β-catenin is preferred."
26185001
sparser
"Consistent with these results, DBC1 increased the interaction of β-catenin with LEF1 in vivo and in vitro ( xref )."
26477307
sparser
"Intermittent PTH is a pathway agonist, thus, the accumulated β-catenin binds to T-cell-specific transcription factor (TCF) and lymphoid enhancing factor (LEF), displacing the Groucho repressor gene, and thereby allowing the transcription of Wnt-specific osteoblast differentiation genes. xref "
28062525
sparser
"Accumulated nuclear β-catenin forms a transcription complex with the T cell-specific transcription factor (TCF) and lymphoid enhancer-binding factor (LEF), triggering target gene transcription."
37211173
sparser
"Furthermore, endogenous interaction between β-catenin and LEF1 was decreased in DBC1 KO SW480 cells ( xref ), suggesting that DBC1 stabilizes the interaction between β-catenin and LEF1 by interacting with β-catenin."
26477307
reach
"Undegraded beta-catenin in SPNs forms a complex with LEF1, enters the nucleus and activates transcription of several oncogenes including cyclin-D1 that is overexpressed in 70-100% of SPNs [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
27267993
reach
"Beta-catenin bound the transcription factors Tcf7 and Lef1, which promoted transcription of targeted genes, as evidenced by the increased gene expression of Jun, Fzd7, and Nlk after TWS119 treatment."
31119051
reach
"The mutations impaired both LEF1 binding to beta-catenin and transcriptional activation, and are the first tumor associated mutations that inactivate Wnt signaling."
16565724
reach
"LEF1, in turn, directly binds to β-catenin and activates the transcription of WNT-related genes, promoting T cell proliferation and differentiation [57], and also regulating chronic inflammation [58, 59]."
37884975
reach
"The activated LEF-1 binds to β-catenin in the nucleus, participates in cell proliferation and migration, and induces the transcription of cyclin D1 and MYC genes, thereby promoting the uncontrolled tu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36603684
sparser
"In the canonical Wnt/β-catenin pathway, nuclear β-catenin is associated with T cell factors and lymphoid enhancer-binding factor 1, leading to the transcriptional activation of target genes that regul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23228700
sparser
"In the context of cancer stemness, interactions between β-catenin and Lef1 transactivate the miR-371–373 cluster that mediates CSC self-renewal [ xref ]."
33817661
sparser
"Together, these results suggest that DBC1 functions as a positive regulator of β-catenin by inhibiting SIRT1-mediated deacetylation and repression of β-catenin through blocking the interaction between β-catenin and SIRT1, thereby enhancing the association of β-catenin with LEF1 and promoting β-catenin-mediated transcription ( xref )."
26477307
sparser
"Taken together, these results show that COX-2 has a conserved Lef1-binding site at the 3′ region of the genomic locus and binding of Lef1 with β-catenin to the site is linked to increased IL-1β-stimul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17945188
reach
"Electrophoretic mobility shift assays (EMSAs) confirmed binding of beta-catenin and Lef1 to the TBE2 site (XREF_FIG)."
24101380
sparser
"The VDR has been shown to interact with β-catenin [22] and Lef1 [23] , two effectors of this pathway."
22008469
sparser
"Consistent with the model in which β-catenin acetylation enhances its binding to LEF1 and leads to increased chromatin occupancy of LEF1 by stabilizing LEF1–β-catenin complex, xref , xref our results further showed that DBC1 enhances the interaction of β-catenin with LEF1 ( xref ) and is required for efficient recruitment of LEF1 and β-catenin to their target chromatin regions ( xref ), probably through protecting β-catenin from SIRT1-mediated deacetylation ( xref )."
26477307
sparser
"The downregulation of APC contributes to nuclear accumulation of β-catenin, which subsequently interacts with the lymphoid enhancing factor/T cell factor (TCF) transcription factor to activate the transcription of target genes including c-Myc, cyclin D1, and matrix metalloproteinase 7 (MMP7) [ xref ]."
30134915
sparser
"In addition, Beta-catenin could bind to and activate Lef1 through the Wnt signalling pathway, and such activation could be facilitated by cadherin junctions xref – xref ."
36243826
reach
"Beta-catenin forms a complex with LEF1 and TCFs and transactivates LEF1 and TCF mediated transcriptions during dorsalization."
17404593
reach
"Beta-catenin is then accumulated in the cytoplasm and translocated to the nucleus and binds to transcription factors LEF-1 and TCF to regulate the transcription of downstream target genes."
22172041
reach
"DACT2 was reported to promote Dvl degradation in a lysosome dependent pathway, and inhibits LEF1 binding to beta-catenin [XREF_BIBR]."
22806826
reach
"Lef1 , a downstream nuclear transcription factor of the canonical Wnt/β-catenin signaling pathway, mediates the Wnt signaling pathway by binding to β-catenin."
37230203
reach
"The potential explanation is that DACT2 promotes Dvl degradation in a lysosome dependent pathway and inhibits LEF1 binding to beta-catenin [XREF_BIBR]."
22806826
sparser
"However, as β-catenin accumulates in the nucleus, Groucho/TLE is displaced from Tcf/Lef by β-catenin binding to C-terminal of Lef1 (residure252-397)."
20858269
sparser
"Lef1 can interact with β-catenin in a transcriptional complex downstream of Wnt signaling xref , and this pathway antagonizes aspects of testis development xref , xref ."
22438826
sparser
"In CRC, ILK induces β-catenin–LEF1 interaction and transactivation, the loss of E-cadherin via induction of EMT-TFs, and the induction of stem cell markers xref – xref ."
30271576
sparser
"Chromatin immunoprecipitation (ChIP) experiments showed that both β-catenin and LEF1 were associated with the Dicer promoter in SW620 cells ( xref )."
39439393
reach
"Nuclear beta-catenin binds to LEF1, displacing the Groucho/TLE repressors, resulting in transcriptional activation of downstream target genes (c-MYC, Cyclin D1, and LEF1) in response to activation of canonical WNT signaling [XREF_BIBR]."
29535816
reach
"beta-Catenin is an important partner in LEF1 transcriptional factor activity, and when LEF1 is bound to beta-catenin, LEF1 transports it to the nucleus."
14691138
reach
"If beta-catenin is bound with LEF1, TGFbeta may not be able to dissociate the complex."
14691138
sparser
"In all SCOUTs the expression of LEF1 was associated with a marked nuclear expression of β-catenin ( xref )."
28664938
reach
"Subsequent EMSA and ChIP analysis confirmed the binding of Ets-1 and LEF-1 and beta-catenin transcription factors to the predicted sites."
22952895
sparser
"In the nucleus β-catenin interacts with the transcription factor lymphoid enhancer factor/T cell factor (LEF/TCF) resulting in expression of target genes such as c-Myc and cyclin D1 that are involved [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22177455
sparser
"To further test the role of Beta-catenin in Lef1 activation, we blocked the interaction between Lef1 and Beta-catenin using the inhibitor iCRT3."
36243826
reach
"How does the binding of beta-catenin to LEF-1 and TCF proteins affect cooperative interactions at a promoter or enhancer?"
10209158
sparser
"Under repressed conditions of COX-2 expression without IL-1β stimulation, basal level Lef1 and β-catenin binding to the Lef1-binding site was observed."
17945188
reach
"Lef1 is a member the Tcf/Lef family of transcription factors and interacts with beta-catenin to up-regulate Wnt target genes."
30872674
reach
"Upon Wnt stimulation, LEF1 or other TCF/LEF family transcription factors associate with beta-catenin, a key cytoplasmic and nuclear mediator of Wnt pathway, and activate Wnt responsive target genes."
21383983
reach
"This increases the pool of cytoplasmic beta-Catenin, which translocates to the nucleus and then binds with LEF-1 to modulate transcription of target genes.A role for the TGF-beta pathway in desmoid ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17601559
reach
"Wnt and beta-catenin pathway activation is characterized by (i) stabilization of cytoplasmic beta-catenin upon receptor engagement by Wnt ligands, (ii) beta-catenin nuclear translocalion, (iii) beta-catenin interaction with lymphoid enhancer binding factor-1 and T-cell factor-1 (LEF and TCF) transcription factors, and (iv) stimulation of target genes XREF_BIBR, XREF_BIBR."
20852629
sparser
"In addition to a significant decrease in the expression of β-catenin, 4-AAQB dose-dependently downregulated the expression of TCF1/TCF (T-cell factor) and LEF1 (Lymphoid enhancer factor) which forms a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32156637
sparser
"Importantly, LEF-1-bound beta-catenin was protected from degradation by APC and axin in SW480 colon cancer cells."
11986304
sparser
"However, upon IL-1β stimulation, both Lef1 and β-catenin binding were significantly increased ( Fig. 2 B)."
17945188
sparser
"Given the oncogenic nature of the LEF-1 feedback loop, therapeutic strategies that block both the interaction between β-catenin and LEF-1 and their association with chromatin could have therapeutic va[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24820952
sparser
"Accumulated β-catenin in the cytoplasm translocates to the nucleus and forms a complex with the transcription factor T cell factor (TCF) or lymphoid enhancer-binding factor 1 (LEF1), replacing Groucho and CtBP corepressors [ xref , xref , xref ]."
37190019
sparser
"Β-catenin accumulates in the nucleus and binds to the T cell factor and lymphoid enhancer factors (TCF/LEF) to regulate gene transcription [ xref ]. xref A,B illustrate the canonical signaling pathway."
37108459
reach
"Functional interaction of beta- catenin with the transcription factor LEF-1."
10503270
sparser
"Given the important role LEF-1 in regulation of β-catenin (Section 4.3 ) it would be interesting if the above compounds also inhibit β-catenin–LEF-1 complexes."
24820952
sparser
"Other therapeutic strategies include preventing β-catenin–LEF-1 complexes from binding to chromatin which in theory may be possible."
24820952
sparser
"DACT2 has been reported to decrease LEF1-β-catenin binding in colon cancer by competing with β-catenin [ xref ]."
30359298
sparser
"Moreover, β-catenin, a protein found at cell–cell adherens junctions, has been shown to play a role in both linking the actin cytoskeleton to the cadherin class of homophilic cell adhesion receptors as well as directly transducing Wnt/ Wingless signals ( xref ; xref ; xref ); Wnt/Wingless signaling results in the nuclear accumulation of a β-catenin-Lef-1 transcription factor complex that binds the promoter regions of several genes (Behrens, et al., 1996; Huber, et al., 1996 b )."
9281590
sparser
"Here, β-catenin interacts with members of the lymphoid enhancer-binding factor (LEF)1/T-cell factor (TCF) transcription factor family and is translocated into the nucleus to stimulate transcription of a wide range of target genes, such as myc and cyclin D1, which are important regulators of epithelial cell proliferation [37,38]."
30743809
sparser
"In sphere cultures, we further observed that ectopic activation of WNT signaling using a β-catenin-LEF1 fusion protein xref (Fig.  xref ) triggered a strong induction of NOTCH signaling (Fig.  xref )."
32066735
reach
"In the presence of WNT signals, LEF1 binds to beta-catenin via its N-terminal beta-catenin-binding domain and promotes context dependent target gene transcription including c-MYC and AXIN2."
27965462
reach
"The Wnt protein binds to Frizzled receptors, followed by the interaction between β-catenin and the LEF1/TCF4 transcription factor."
39300161
sparser
"Because it has been reported that LEF-1 interacts with β-catenin outside the amino-terminal domain, namely, in the middle part of the molecule, the possible participation of β-catenin has not been formally ruled out."
29998192
sparser
"Drugs designed to disrupt LEF-1–β-catenin transcription factor complexes hold great promise for treating cancers stemming for aberrant Wnt signaling, particularly due to the oncogenic nature of the LE[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24820952
sparser
"Nonetheless, there is a report that in addition to the well-established amino-terminal β-catenin–binding domain of LEF-1, β-catenin binds to another site of LEF-1: residues 150–175 xref ."
29998192
sparser
"In our previous study, we have reported that not only wild-type LEF-1 but also an amino terminus–deleted mutant LEF-1, ΔNLEF-1, which cannot interact with β-catenin, has an ability to induce EMT xref ."
29998192
sparser
"Because EpICD has been shown to form β-catenin-mediated transcription complexes with LEF1 that is also overexpressed in endometrial tumors ( xref ), we examined LEF1 binding profiles in RL95-2 cells by ChIP-seq ( xref )."
27569206
sparser
"Despite the finding that transcription factor LEF-1 interacts with β-catenin to activate their target genes, such as EMT-related gene SNAI2 xref , and induces EMT, we found that not only full-length LEF-1 but also LEF-1 lacking the N-terminal β-catenin–binding domain can induce EMT."
29998192
reach
"Expression of the MYF5 gene is regulated directly by CTNNB1 and LEF1 binding and transcriptional co-activation of the MYF5 epaxial enhancer XREF_BIBR."
28165492
sparser
"This ultimately coincides with the nuclear translocation and binding of β-catenin to the lymphoid enhancer-binding factor/T-cell-specific transcription factors (LEF/TCF), family of transcription facto[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23274128
sparser
"LEF1, in turn, directly binds to β-catenin and activates the transcription of WNT-related genes, promoting T cell proliferation and differentiation [ xref ], and also regulating chronic inflammation [ xref , xref ]."
37884975
sparser
"Rac1 also enhances the nuclear formation of the β-catenin and LEF-1 complexes, providing the basis for the role of Rac1 in augmenting transactivation of Wnt target genes [ xref ]."
32178475
reach
"The binding of unphosphorylated beta-catenin to Lef1 in the nucleus to form the transcription activator was modeled using a mass action law (Eq2.11 in the Additional file XREF_SUPPLEMENTARY)."
23602012
reach
"Research by Juan Galceran et al. [XREF_BIBR] confirmed that Lef1 binds to beta-catenin in the nucleus to form the Lef1, beta, and catenin complex that activates the expression of endogenous Dll1 in fibroblasts."
23602012
sparser
"Subsequent transforming growth factor (TGF)-beta3 signaling increases LEF-1 gene expression causing formation of beta-catenin-lymphoid enhancer factor (LEF)-1 complexes that initiate EMT."
18799618
reach
"Finally, β-catenin binds to TCF/LEF1 to replace the transcriptional suppressor Groucho in the target gene promoter, thereby regulating the expression of downstream target genes (c-Myc, Cyclin D1, etc.)."
32038289
sparser
"On the contrary, we observed an inhibition of HIV-1 reactivation in GS143 treated CD4+ T cells by ethacrynic acid [ xref ], which interferes with the association β-catenin with LEF-1 suggesting the importance of β-catenin-LEF-1 interaction in GS143-mediated HIV-1 reactivation ( xref )."
40168443
biogrid
No evidence text available
8757136
biogrid
No evidence text available
8757136
reach
"The zinc-finger transcription factor snail is activated when β-catenin translocates to the nucleus and binds with TCF/LEF-1 to form a functional complex, which results in E-cadherin down-regulation an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28712972
reach
"LEF1, in turn, binds to the promoter regions of at least two direct target genes (Lmx1a and Fgf20) and together with beta-catenin appears to activate their transcription in the mutant VM."
26049140
sparser
"Inside the nucleus, β-catenin interacts with T cell transcription factor (TCF) and lymphoid enhancer factor (LEF) and induces transcription of β-catenin target genes [ xref ]."
33049989
sparser
"Thus, the differential dependence on AML LSCs versus HSCs offers the opportunity to reach LSCs preferentially, impairing the LEF1-β-catenin interaction and, consequently, inhibiting the growth of AML,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33716201
reach
"In the canonical pathway, Wnt binding leads to the stabilization of the transcription factor β-catenin, which enters the nucleus and interacts with lymphoid enhancer factor 1 (LEF-1) to regulate Wnt p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19643084
sparser
"The cytoplasmic tails of N- or E-cadherin colocalized with beta-catenin in the nucleus, and suppressed the constitutive LEF-1-mediated transactivation, by blocking beta-catenin-LEF-1 interaction."
9860970
sparser
"The critical nature of cell-cell adhesions (specifically E-cadherin binding) to control the oncogenic activity of β-catenin is underscored by studies showing that binding of β-catenin to E-cadherin prevents β-catenin nuclear localization and β-catenin/LEF-1-mediated transactivation xref , xref , xref , which β-catenin binding with LEF-1 can be out-competed by E-cadherin xref , as well as studies showing that E-cadherin can limit the transforming properties of activating β-catenin mutations xref , xref , xref ."
39605508
reach
"In the nucleus, beta-catenin binds to and activates the TCF/LEF transcription factor complex to induce the expression of wnt-pathway genes, such as, EphB2, EphB3, TCF4 and LEF1."
19956666
sparser
"In addition, Smad4 can be immunoprecipitated in a complex with β-catenin and Lef1 from cultured cells [85] and Smad4 binding sites in the Xtwn promoter are required for optimal activation in response [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16356740
reach
"Binding of beta-catenin to cadherin, APC protein, and LEF-1 indicates divergent functions of beta-catenin."
9199178
reach
"In addition, beta-catenin also translocates into the nucleus and interacts with LEF-1 and TCF-4 at the COX2 promoter to activate the transcription of COX2."
28587064
reach
"Regarding the putative role of the LEF1 gene in hernia, it has been shown that the LEF1 protein constitutively associates with beta-catenin which mediates the action of the anti-mullerian hormone (AMH) [XREF_BIBR], itself involved in the gubernaculum swelling reaction that occurs during the first phase of testicular descent [XREF_BIBR, XREF_BIBR]."
25886970
reach
"In response to a canonic Wnt signal, β-catenin accumulates in the nucleus and binds Lef-1 or other Lef/Tcf family transcription factors, and regulates the transcription of downstream genes."
22521245
sparser
"Wnt/β-catenin also enhances EMT: addition of Wnt-1 to normal epithelial cell lines stabilizes the complex between cytoplasmic β-catenin and lymphoid enhancing factor-1 (LEF-1) that then transports to the nucleus, leading to EMT, and Kim et al. demonstrated that overexpressed LEF-1 dramatically promoted EMT [ xref ]."
37510988
sparser
"We conclude that Wnt3a signaling stimulates the robust assembly of LEF-1:β-catenin complexes near hESC mesendodermal genes."
25936800
sparser
"In 2006, Medici, working with Hay and Goodenough [176] , showed that complete loss of CDH1 expression and consequent full EMT required Smad signaling that stimulated the formation of β-catenin complex[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37852342
reach
"In NLK-knockout cells, there is a constitutive interaction between Lef1 and beta-catenin, which could explain the elevated Tcf/Lef promoter activity in these cells."
27903773
sparser
"A high proportion of human sebaceous adenomas have amino acid substitutions in the N-terminus of Lef1, which impair the binding of Lef1 to β-catenin resulting in the inhibition of β-catenin-dependent transcription of Wnt target genes [ xref ]."
22960253
sparser
"As expected, Wnt3a greatly increased binding of both LEF-1 and β-catenin, predominantly to pre-existing enhancer sites."
25936800
reach
"Further, a significant difference in the interaction between Lef1 and beta-catenin was seen when wild-type cells were compared with knockout cells (XREF_FIG)."
27903773
sparser
"Lef1 interacts with β-catenin to activate the transcription of the Wnt target genes [ xref , xref ]."
36140843
sparser
"This TNKSi insensitivity is conferred by β-catenin’s association with LEF1 and BCL9-2/B9L, which accumulate during Wnt stimulation, thereby providing a feed-forward loop that converts transient into chronic β-catenin signaling."
24419084
sparser
"Cell (Cambridge, Mass.) 111, 251–263 *Behrens, J., von Kries, J.P., Kuhl, M., Bruhn, L., Wedlich, D., Grosschedl, R. and Birchmeier, W. (1996) Functional interaction of β-catenin with the transcription factor LEF-1."
15715524
sparser
"The coordinated binding of β-catenin and LEF-1 to enhancers is reminiscent of the cooperative binding of recombinant LEF-1 and β-catenin proteins on chromatin templates in vitro ( xref )."
25936800
sparser
"Because Wnt3a preferentially induces the full-length form of LEF-1 ( xref ), which includes the N-terminal β-catenin interaction domain, this mechanism ensures mutually interdependent binding of LEF-1 and β-catenin to chromatin and enables rapid and synchronous activation of downstream target genes."
25936800
sparser
"OTUD7B Promotes the Interaction between LEF1 and β-Catenin."
37371581
sparser
"We further investigated whether the increased nuclear translocation of LEF1 by OTUD7B leads to enhanced transcriptional activity by promoting the interaction between LEF1 and β-catenin."
37371581
sparser
"Indeed, functional activation of β-catenin-LEF1 was confirmed in the nail matrix just above the RSPO4 expressing mesenchyme (Fig. xref )."
34099859
sparser
"MYC promotes the interaction between LEF1 and β-catenin by directly targeting LEF1, activating the expression of PPARβ/δ and Acyl CoA dehydrogenase 9(ACAD9)."
39875357
sparser
"This clearly demonstrates that OTUD7B promotes the nuclear localization of LEF1 and enhances the interaction between LEF1 and β-catenin in the nucleus, resulting in enhanced transcriptional activation."
37371581
sparser
"However, EpICD does not only interact with β-catenin and Lef-1, it also binds to Lef-1 consensus sites in the promoter of Wnt target genes such as cyclin D1."
24944998
sparser
"In relation to this, it is notable that although lymphoid enhancer factor/TCF7 transcription factors associate with β-catenin in canonical wnt signaling leading to the transcription of its target genes, SATB1 has recently been shown to competitively influence the TCF7 binding to β-catenin ( xref )."
20467038
sparser
"Central regions of Tcf7s and Lef1 interact weakly with β-catenin and strongly with co-repressors, including Hdacs and Tle proteins."
22079544
reach
"MYC promotes the interaction between LEF1 and β-catenin by directly targeting LEF1, activating the expression of PPARβ/δ and Acyl CoA dehydrogenase 9(ACAD9)."
39875357
sparser
"The binding of unphosphorylated β-catenin to Lef1 in the nucleus to form the transcription activator was modeled using a mass action law (Eq 2.11 in the Additional file xref )."
23602012
reach
"MMP-2 was observed to dissociate the E-cadherin–β-catenin complex and increase the nuclear translocation and binding of β-catenin with lymphoid enhancer-binding factor 1 (LEF1) for the expression of fibrotic genes, such as α-SMA and collagen I [57]."
35743056
sparser
"Specificity of the small molecules was demonstrated by coimmunoprecipitation experiments for the lef-1/beta-catenin interaction."
20360943
sparser
"The stabilized β-catenin interacts with the T-cell factor (TCF) and lymphoid enhancer factor (LEF) transcription factors in a manner that displaces genetic repressors and recruits coactivators."
17208432
sparser
"The GSK3-Axin2 degradation complex is in antiphase with the β-catenin-Lef1 transcriptional activator, because it degrades β-catenin and thus inhibits the formation of the β-catenin-Lef1 complex."
23602012
sparser
"Additionally, since LEF1 and β-catenin interact as two essential transcriptional components of the Wnt signaling pathway, OTUD7B may facilitate the nuclear localization of both LEF1 and β-catenin as a protein complex."
37371581
sparser
"In line with our previous findings that DBC1 enhances LEF1-β-catenin complex formation on WREs of β-catenin targets including a Wnt-inducible transcription factor PROX1 involved in colon cancer progression xref , DBC1 increases LEF1-β-catenin interaction on the MACC1 enhancer, and loss of DBC1 reduced the occupancy of LEF1, TCF4, and β-catenin on the MACC1 enhancer."
30082743
sparser
"Coimmunoprecipitation showed a selective disruption of beta-catenin/lef-1 interaction."
20360943
reach
"Based on these insights, new therapeutic opportunities have been explored by investigators who show that small-molecule WNT-pathway inhibitors, which inhibit the interaction between beta-catenin and LEF1, selectively induce cell death in AML cell lines and primary AML blasts [XREF_BIBR]."
27571104
sparser
"Research by Juan Galceran et al. [ xref ] confirmed that Lef1 binds to β-catenin in the nucleus to form the Lef1-β-catenin complex that activates the expression of endogenous Dll1 in fibroblasts."
23602012
reach
"In the nucleus beta-catenin binds to Lef1 and TCF transcription factors to initiate gene activation.The importance of Wnt signaling in bone development became obvious following the discovery of gain- [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19389391
sparser
"In NLK-knockout cells, we observed sustained interaction between Lef1 and β-catenin, leading to elevated luciferase reporter of β-catenin/Lef1–mediated transcriptional activation."
27903773
reach
"Then, β-catenin binds to LEF-1 to regulate Wnt-like, ERE-independent, gene transcription and also binds to ERs to regulate ERE-dependent gene transcription."
19778547
biogrid
No evidence text available
9419974
reach
"We performed ChIP assay and found that ARA014418 treatment increased the levels of co-immunoprecipitated cis-elements of LEF1/TCF-1 and LEF1/TCF-2 by β-catenin (Figure 7A), suggesting inhibition of GSK-3β enhances the binding of β-catenin to LEF1/TCF elements.To determine the effect of ARA014418 on PME-1 expression in primary cultured cortical neurons, we treated the neurons with various concentrations of ARA014418 for 4.5 hr and analyzed mRNA level of PME-1 by qPCR."
31714894
sparser
"Interaction of β-catenin with transcription factors of the lymphoid enhancer-binding factor/T cell factor family in the nucleus subsequently regulate the transcription of Wnt target genes ( xref )."
19723499
reach
"In the embryonic ectoderm, the TCF family members LEF1, TCF4, and TCF3 are phosphorylated after Wnt stimulation.46 Of note, at early stages of T-cell development, TCF1 is rapidly induced by NOTCH signaling and binds to β-catenin, which is activated by the Wnt cascade induced by Frizzled and LRP5/6 receptors interacting with extracellular Wnt ligands, to promote chromatin accessibility.47–49 Here, our data showed that TCF1 displayed increased phosphorylation after LDL stimulation."
38272565
biogrid
No evidence text available
9419974
sparser
"Behrens J, Von Kries JP, Kuhl M, Bruhn L, Wedlich D, Grosschedl R, et al. Functional interaction of β-catenin with the transcription factor LEF-1."
12975039
sparser
"Elevated beta-catenin levels in colorectal cancer caused by mutations in beta-catenin or by the adenomatous polyposis coli molecule, which regulates beta-catenin degradation, result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of mostly unknown target genes."
10318916
reach
"However, it has also been reported that the inhibition of interaction between beta-catenin and Lef-1 caused HFSCs to differentiate into sebocytes XREF_BIBR, XREF_BIBR."
25076848
reach
"The two pathways were found to converge at the promoter of Xtwn, where Smad4, beta-catenin, and Lef1 form a complex and bind to adjacent regulatory elements to co-regulate Xtwn expression."
19002158
reach
"Together, the data obtained so far suggested that estradiol might activate a beta-Catenin and LEF -1 complex that is similar to that activated by Wnt in other systems."
19360103
sparser
"We confirmed the β-catenin and Lef1 interaction by overexpressing HA-tagged Lef1 alone or β-catenin together."
17445771
sparser
"The interaction between Lef1 and β-catenin was clearly detected ( Fig. 4 B, lower)."
17445771
sparser
"Studies show that low levels of E-cadherin mimics Wnt signaling, allowing free β-catenin to associate with LEF-1, a transcription factor, in the nucleus and contribute to cancer cell growth [ xref ]."
40001961
reach
"The bioinformatic analysis revealed an array of putative nuclear factor-binding sites, including two potential LEF1/TCF like elements located on -349 bp – -333 bp and +19 bp – +35 bp (Figure 2A), on which β-catenin acts as co-activator of TCF transcription factors to regulate the transcription of target genes.To determine whether β-catenin binds to the LEF1/TCF elements of PME-1 promoter, we performed the ChIP assay in SH-SY5Y cells."
31714894
reach
"We investigated whether inhibition of GSK-3β affects the binding of β-catenin with LEF1/TCF cis-elements."
31714894
sparser
"The association of Ep-ICD with FHL2 and Wnt pathway components - β-catenin and Lef-1 forms a nuclear complex that binds DNA at Lef-1 consensus sites and induces gene transcription, leading to increased cell proliferation and has been shown to be oncogenic in immunodeficient mice [ xref ]."
20579375
sparser
"The activated LEF-1 binds to β-catenin in the nucleus, participates in cell proliferation and migration, and induces the transcription of cyclin D1 and MYC genes, thereby promoting the uncontrolled tu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36603684
biogrid
No evidence text available
21743491
reach
"Further, Shtutman and colleagues 83 demonstrated that the beta-catenin and LEF -1 complex binds to the cyclin D1 promoter, and the promotion of cyclin D1 expression by beta-catenin is inhibited by tra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11839557
reach
"93 The changes in COX-2 described in the latter study model are also associated with increased beta-catenin and LEF -1 complex formation."
11839557
sparser
"Accumulated β-catenin within the nucleus can interact with T-cell factor-lymphoid enhancer-binding factor (Tcf-Lef) to enable Wnt-respondent gene transcription, containing c-Myc and Cyclin D1, eventually causing the alterations in the proliferation. xref MMP-7 is transcriptionally modulated by β-catenin and also one of primary downstream regulator of canonical Wnt signaling reported in diverse cancers, such as ovarian endometrial carcinoma xref , intestinal adenoma xref and colorectal cancer."
38378037
reach
"We found that the promoter of human PME-1 contains two LEF1/TCF cis-elements, which act as enhancers to promote the expression of PME-1 and that binding of β-catenin to LEF1/TCF cis-elements enhances the expression of PME-1."
31714894
sparser
"The activation of Wnt/β-catenin signaling allows the accumulated β-catenin to bind T-cell-specific transcription factor and lymphoid enhancing factor, leading to the transcription of Wnt-specific osteoblast differentiation genes [ xref ]."
38877549
sparser
"In addition, LEF1 can form the heteromeric protein complex with β-catenin to mediate Wnt/β-Catenin pathway transcription."
36603684
biogrid
No evidence text available
21743491
sparser
"And the depletion of FOXG1 diminished the binding of β-catenin to the LEF-1 promoter (Fig. xref e)."
31771611
sparser
"Co-immunoprecipitation experiments have revealed that ECA can directly bind to the LEF-1 protein in SW480 colon cancer cells [ xref , xref ] and destabilize the LEF-1-β-catenin complex, thereby interfering with its formation with β-catenin."
37047688
sparser
"YAP downregulation has been shown to sensitize BRAFi-resistant melanoma cells to MAPK pathway inhibitors, and YAP and Wnt signaling (via β-catenin-LEF1) coregulate apoptosis of acquired MAPKi-resistant melanoma ( xref )."
34462276
sparser
"Binding of Wnt to FZD and LRP triggers stabilization and translocation of β-catenin into the nucleus, where β-catenin associates with T cell factor or lymphoid enhancer factors to activate Wnt target genes."
35569814
sparser
"Lef-1 and β-catenin form a functional transcription factor complex as a result of Wnt signaling, and their overexpression can bypass a Wnt signal ( Behrens et al., 1996 )."
12039047
sparser
"This could be explained by a likely association of over-expressed Lef-1 protein with endogenous β-catenin."
12039047
sparser
"No binding of LEF-1 or β-catenin was observed at distal (5 kB) upstream sites (data not shown)."
17113388
sparser
"SOX9 has been shown to interact with β-catenin [ xref ], which in turn binds to LEF1 [ xref ]."
35191090
sparser
"DACT2 was reported to promote Dvl degradation in a lysosome-dependent pathway, and inhibits LEF1 binding to β-catenin [ xref ]."
22806826
sparser
"SOX9 physically interacts with β-catenin [ xref ], which in turn binds to LEF1 [ xref ]."
35191090
sparser
"Upon Wnt stimulation, LEF1 or other LEF/TCF family transcription factors associate with β-catenin, a key cytoplasmic/nuclear mediator of the Wnt pathway, to activate Wnt-responsive target genes."
21880414
sparser
"This implies that β-catenin and Lef-1 function through the formation of a β-catenin-Lef-1 complex during the cyclical growth of hair follicles."
22521245
sparser
"Jerky also recruits β-catenin to chromatin, and promotes the association of β-catenin and LEF1, resulting in the induction of Wnt target gene expression.( xref , xref ) We found that RUNX3 increases the occupancy of the TCF4/β-catenin complex in KatoIII cells, suggesting that RUNX3 plays a role in the stabilization of the TCF4/β-catenin complex on the Wnt target gene promoter like TBL1 and Jerky."
24447505
sparser
"In vertebrates, the conserved Wnt signalling cascade promotes the stabilization and nuclear accumulation of β-catenin, which then associates with the lymphoid enhancer factor/T cell factor proteins (LEF/TCFs) to activate target genes."
20126258
reach
"Wnt factors including beta-catenin, glycogen synthase kinase (GSK3) beta, the lymphoid enhancer binding factor 1 (LEF1) and cyclin D1 have been identified as interacting with the androgenic signaling axis [XREF_BIBR, XREF_BIBR]."
23667383
sparser
"Accumulated β-catenin associates with members of the lymphoid enhancer-binding factor/T-cell factor (LEF/TCF) family to regulate specific target genes such as c-myc, cyclin D1, CD44, and Axin2 xref ."
27668402
reach
"Another study showed that SP5 and SP8 both assist the binding of β-catenin to LEF1 upon Wnt activation."
34552611
sparser
"Although these variations in LEF1 expression had no impact on the expression and the phosphorylation of β-catenin, the formation of LEF1/phospho-β-catenin complex was positively associated with the ex[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21880414
sparser
"On the other hand, based on the decreased expression of β-catenin signaling target genes such as Padi1 , Dix , and Tubb3 in Vdr -cKO mice, VDR is thought to form a complex with β-catenin and Lef1 to activate β-catenin signaling and promote keratinocyte proliferation [ xref ]."
39796272
reach
"These results provide evidence that Lef1 promoted the differentiation of bulge stem cells into a hair fate and interacted directly with beta-catenin."
23630438
reach
"To confirm that the compounds inhibit LEF-1 and beta-catenin DNA binding, EMSA assays were performed with immunoprecipitated beta-catenin and a 32 P labeled double stranded deoxyoligonucleotide (5 '-CCCTTTGATCTTACC-3 ') containing the LEF-1 consensus binding site in the presence of varied concentrations of the compounds."
19216569
reach
"We also report that Lef1 could bind to beta-catenin directly and translocate it into the nucleus, resulting in the activation of the Wnt signaling pathway."
23630438
sparser
"Chromatin immunoprecipitation (ChIP) analysis also showed that the T-cell factor (TCF)4, which is one of the transcription factors of the lymphoid enhancer factor (LEF)/TCF family that binds to stabilized β-catenin to regulate Wnt signalling xref , is bound to the promoter of Irs1 xref ."
27708333
reach
"In Wnt signaling pathway, LEF1 interactions with beta-catenin transcriptionally activate Wnt regulated genes."
38113273
reach
"There is evidence that beta-catenin can act as a nuclear import receptor to induce the nuclear translocation of Lef1 XREF_BIBR, indicating a close interaction between Lef1 and beta-catenin."
23630438
reach
"Because LEF-1 possesses its own NLS (Huber et al., 1996) and is able to interact with beta-catenin, we asked whether glucose favored LEF-1 and beta-catenin interaction as a possible mechanism for the [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
reach
"The use of this type of library has identified erythropoieitin (EPO) mimetics (promote EPO receptor dimerization), inhibitors of Myc and Max dimerization, inhibitors of LEF-1 and beta-catenin dimerization, inhibitors of the binding of the protease MMP-2 to alphavbeta3 integrin, and inhibitors of HIV protease."
21183197
reach
"Thus, the availability of LEF-1 may be critical for LEF-1 and beta-catenin interaction and nuclear accumulation.Given that acetylation of beta-catenin through p300 or CBP (Wolf et al., 2002; Xu and Ki[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
sparser
"In the nucleus, β-catenin interacts with T-cell-specific transcription factor (TCF) and lymphoid enhancer-binding factor (LEF) families to drive the transcription of target genes [29] ."
28865751
reach
"Whether LEF1 interactions with beta-catenin and/or SMADs is required for EBV lytic replication needs to be further examined.LEF1 repressive transcriptional activity is also potentiated by interaction with negative regulators Groucho/TLE that recruit HDACs to facilitate chromatin condensation [86]."
38113273
reach
"Thus, unlike polarized cells, in mesenchymal cells LEF-1 and beta-catenin may form complexes within the nucleus."
10629227
reach
"Collectively, the results indicate that combined glucose and LiCl treatment increases p300-LEF-1-beta-catenin interaction while preventing SIRT1-LEF-1 and SIRT1-beta-catenin interaction and suggest th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
reach
"LEF1 binds to β-catenin to regulate the expression of downstream molecules, thereby regulating the signal pathway (42)."
34485109
sparser
"These results indicated that the interaction of LEF1 with β-catenin plays a key role to promote the synergy of DAC and PTX against RCC cells."
21880414
signor
"Activated dvl binds and inhibits the phosphorylation of beta catenin by gsk3beta/alfa, blocking beta catenin degradation), so that beta catenin accumulates and translocates to the nucleus, where it interacts with the t cell specific factor (tcf)/lymphoid enhancer binding factor 1 (lef-1) transcription factor and induces the transcription of target genes such as c-jun, c-myc, and cyclin d1"
15735151
reach
"To activate target gene expression, TCF-1 and LEF-1 must first associate with beta-catenin."
23041833
sparser
"In order to block Wnt/β-catenin signalling, we expressed an N-terminally truncated form of Lef1 (ΔNLef1), which does not bind β-catenin and acts as a dominant-negative inhibitor, in the epidermal basa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18805411
reach
"Whereas ectoptic WT beta-catenin bound p300 upon exposure of the cells to both glucose and LiCl (like endogenous beta-catenin), conditions that promote LEF-1 and beta-catenin interaction and nuclear a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
reach
"Given that p300 binds the C terminus of beta-catenin (Hecht et al., 2000) more than 300 amino acids away from the region missing in Delta19 beta-catenin (arms 5-6), our results suggest that LEF-1 and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
reach
"Thus, the LEF-1 and beta-catenin interaction promoted by the combination of glucose and LiCl appears critical for stabilizing p300 interaction and nuclear retention.To test whether beta-catenin acetyl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23273980
reach
"Although the precise molecular mechanisms have not been explored here, we view it as probable that SIRT1 acts as a competitor for interaction between LEF-1 and beta-catenin."
23273980
reach
"Our results show that Wnt signaling is critically involved in the positioning of the premuscle masses, as their position highly correlates with the experimentally induced position of Tcf4 , a downstre[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17005177
reach
"3D complex structures show that CDH1, LEF1 and TCF7L2 bind to beta-catenin."
27928465
sparser
"Finally, crystal structure of β-catenin ARM repeats 2–10 with a bound part of Lef1 β-catenin-binding domain (β-catenin-BD) revealed that Lef1 interacts with β-catenin in an analogous manner as other members of TCF family."
32486423
reach
"Beta-Catenin and LEF-1 complexes can activate reporter gene expression in a transformed T-lymphocyte cell line (Jurkat) but not in normal T lymphocytes, even though both factors are nuclear."
10330189
sparser
"In the nucleus β-catenin associates with members of the Lymphoid Enhancing Factor/ T-cell Factor (LEF/TCF) family of transcription factors, and induces the expression of genes that regulate cell proli[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27090017
sparser
"The ability to disrupt LEF-1–β-catenin engagement to chromatin could cause the release and export of the entire β-catenin–LEF-1 complex from the nucleus."
22433990
sparser
"Examination of individual NC genes confirmed the association of LEF1 and CTNNB1 with tissue-specific enhancers."
33852891
sparser
"AEG-1 facilitated β-catenin translocation into the nucleus by forming a complex with LEF1 and β-catenin, subsequently activating Wnt signaling downstream genes."
27903708
sparser
"We selected 30 genomic regions that interact with promoters of NC genes and are associated with both LEF1 and CTNNB1."
33852891
sparser
"To define the regulatory features of Wnt-associated elements, we examined how LEF1 and CTNNB1 interact with Axud1E1 , an enhancer directly activated by canonical Wnts ( xref )."
33852891
reach
"Hes-1 has recently also been identified as a direct transcriptional target of beta-catenin and Tcf-4-dependent Wnt signaling in CRC XREF_BIBR and our EMSA data supports this notion by clearly identifying Hes-1 -528 as a binding site for the beta-catenin and Lef -1 complex (XREF_SUPPLEMENTARY)."
21437251
sparser
"Insulin stimulation increased the binding of LEF1 and β-catenin while reducing FoxO1 binding."
25091498
sparser
"When LEF-1 interacts with CTNNB1, it leads to upregulation of LEF-1 in SPN."
30283597
sparser
"In this scenario, β-catenin/TCF-1/TCF-4 complexes bind and transactivate the LEF-1 promoter ( Hovanes et al., 2001; Atcha et al., 2003 ) generating newly synthesized LEF-1, which can then bind β-caten[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22433990
sparser
"Finding ways to not only block the interaction between LEF-1 and β-catenin, but to disrupt its engagement to chromatin could be of therapeutic value."
22433990
sparser
"In principle this is possible, as it was recently shown that β-catenin–LEF-1 complexes were not retained in the nucleus when the chromatin-binding domain of LEF-1 was mutated ( Sharma et al., 2012 )."
22433990
sparser
"The challenge will be to ensure that these agents are non toxic and highly specific for β-catenin–LEF-1 complexes in cancer cells, leaving normal cellular processes intact."
22433990
reach
"Chibby (Cby) inhibits Wnt and beta-catenin-mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of beta-catenin) to bind to beta-catenin."
25175341
reach
"Upon accumulation, beta-catenin interacts with Lef-1 and Tcfs to generate a functional transcription factor complex (Behrens et al. 1996; van de Wetering et al. 1997)."
9845363
reach
"In addition, an antisense DeltaNp63alpha construct (DeltaNp63alpha-AS) reduced luciferase activity mediated by the endogenous beta-catenin and Lef -1 complex, while TAp63alpha had no effect on this ac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12086851
sparser
"Further, a significant difference in the interaction between Lef1 and β-catenin was seen when wild-type cells were compared with knockout cells ( xref )."
27903773
reach
"Functional interaction of beta-catenin with the transcription factor LEF-1."
12975039
sparser
"Further, a more recent study suggests that Wnt-mediated Rac1 activation not only induces nuclear translocation, but also enhances β-catenin and LEF-1 binding in the nucleus ( xref )."
36248967
biogrid
No evidence text available
37566052
sparser
"Wnt/β-catenin pathway activation is characterized by (i) stabilization of cytoplasmic β-catenin upon receptor engagement by Wnt ligands, (ii) β-catenin nuclear translocalion, (iii) β-catenin interaction with lymphoid enhancer-binding factor-1/T-cell factor-1 (LEF/TCF) transcription factors, and (iv) stimulation of target genes xref , xref ."
20852629
sparser
"However, this increase in NRARP:LEF1 interactions only translated into increased Wnt signaling activity in Loucy cells as determined by the increase in LEF1:β-catenin interactions in these cells upon NRARP overexpression (Fig. xref g and Supplementary Fig. xref , xref )."
31586130
reach
"We demonstrated that LEF1 and β-catenin bind to and regulate MIR17HG promoter activity, and that activation of the Wnt/β-catenin pathway increases the MIR17HG promoter activity as well as the endogenous expression of miR-17-92 cluster, whereas inactivation of the Wnt/β-catenin pathway reduces miR-17-92 cluster expression."
31191623
sparser
"Here, the stable β-catenin interacts with the lymphoid enhancer factor/T cell factor (LEF/TCF) and is translocated into the nucleus as a complex of β-catenin/LEF/TCF to stimulate target gene transcription by displacement of groucho-HDAC co-repressors xref , xref ."
23490077
reach
"Moreover, a LEF-1 and TCF recognition site has been identified upstream of these elements, which was shown to bind to a beta-catenin and LEF -1 complex in vitro (Huber et al. 1996b)."
10629227
sparser
"This approach has been used to inhibit WNT signaling with a β-catenin-ENGR repressor and relies on β-catenin's association with the activator LEF-1, which provides the DNA-binding and promoter targeti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11164187
reach
"Taken together, all these data suggest that LEF1 and β-catenin specifically bind to and regulate the MIR17HG promoter."
31191623
reach
"To validate whether LEF1 and β-catenin interact to regulate MIR17HG, we determined the effect of LEF1 and β-catenin interaction on MIR17HG promoter activity."
31191623
reach
"Our results showed that LEF1 and β-catenin could bind to and regulate the MIR17HG promoter (Figure 2)."
31191623
reach
"The upregulation of the above genes takes place in a direct manner, as we demonstrated, conserved LEF binding sites in MMP-9, 13, 14, ADAMTS-5, and COL10A1 promoters, responsible for their promoter activity and is associated directly with the beta-catenin and LEF -1 complex."
22513174
reach
"In both Xenopus and Drosophila, LEF-1 and TCF-1-like proteins bind beta-catenin and appear to translocate with it to the nucleus (Behrens et al. 1996; Riese et al. 1997; van de Wetering et al. 1997)."
9288749
sparser
"In addition, our data shows that NRARP overexpression increases the number of NRARP:LEF1 interactions which in T-ALL cells with low levels of NICD1 translates into more LEF1:β-catenin interactions."
31586130
reach
"However, there are clear discrepancies with the reported nuclear localisation of both β-catenin and the key canonical transcription factor lymphoid enhancer binding factor 1 (LEF1) in the pre-cortex [21,22] and the DP [20,23]."
29738529
reach
"It remains to be determined whether beta-catenin and LEF -1 complexes recognize this site also in vivo and whether they influence the expression of E-cadherin in a positive or negative fashion."
10629227
sparser
"Currently, there are drugs approved by the Food and Drug Administration (FDA) for other medical conditions that target the Wnt/β-catenin pathway, including niclosamide (a Frizzled receptor inhibitor), pimozide (a β-catenin inhibitor), and ethacrynic acid (a β-catenin-LEF1 inhibitor)."
38892410
sparser
"Behrens J, von Kries JP, Ku¨ hl M, Bruhn L, Wedlich D, Grosschedl R et al. Functional interaction of beta-catenin with the transcription factor LEF-1."
22716317
reach
"HQ blocked nuclear translocation of beta-catenin and lymphoid enhancer binding factor 1 (LEF-1), and importantly, HQ also reduced the interaction of beta-catenin and LEF-1 in the nucleus."
28886972
reach
"Taken together, our results suggest that HQ induced hematotoxicity in bone marrow mononuclear cells is associated with dysregulation of Akt/GSK-3beta/beta-catenin signaling due to the dissociation of beta-catenin and LEF -1 complex, and LiCl and IGF-1 may be two potential agents to ameliorate HQ induced hematotoxicity."
28886972
reach
"In this scenario, β-catenin/TCF-1/TCF-4 complexes bind and transactivate the LEF-1 promoter ( Hovanes et al., 2001; Atcha et al., 2003 ) generating newly synthesized LEF-1, which can then bind β-caten[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22433990
sparser
"In the absence of E-cadherin, there was more interaction between β-catenin and LEF-1 than in E-cadherin wild-type cells."
36248967
reach
"Finding ways to not only block the interaction between LEF-1 and β-catenin, but to disrupt its engagement to chromatin could be of therapeutic value."
22433990
sparser
"Moreover, CK1-dependent phosphorylation in contrast to CK2 disrupts the association of beta-catenin and LEF-1 but does not impair DNA binding of LEF-1."
15747065
reach
"The ability to disrupt LEF-1–β-catenin engagement to chromatin could cause the release and export of the entire β-catenin–LEF-1 complex from the nucleus."
22433990
reach
"When beta-catenin binds TCF and Lef1, it can regulate the transcription of Wnt target genes [XREF_BIBR]."
27999816
reach
"The challenge will be to ensure that these agents are non toxic and highly specific for β-catenin–LEF-1 complexes in cancer cells, leaving normal cellular processes intact."
22433990
reach
"Interactions between beta-catenin and LEF-1 and TCF, APC and conductin and axin are essential for wnt controlled stabilization of beta-catenin and transcriptional activation."
10966653
reach
"While beta-catenin can bind directly to both AR and Lef1, GRIP1 can only bind directly to AR; the ability of GRIP1 to associate with and function as a coactivator for Lef1 is entirely dependent on the presence of beta-catenin."
14638683
sparser
"The Lef1 distal region is bound by β-catenin in WNT-stimulated ESCs but not in normal ESC culture conditions [ xref E, compare β-catenin (WNTa) versus β-catenin (control); data from xref ]."
36102628
reach
"As many of these structures were also stained by an affinity purified anti-LEF-1 antibody, they likely represent functional beta-catenin and LEF -1 complexes."
10629227
sparser
"Using recombinant proteins, we found that E-cadherin and lymphocyte-enhancer factor-1 (LEF-1) form mutually exclusive complexes with beta-catenin; the association of beta-catenin with LEF-1 was competed out by the E-cadherin cytoplasmic domain."
10085258
reach
"WNT signaling in CNS vasculature maintains the blood-brain barrier (BBB) and it is both mediated by and up-regulates the transcription factor LEF1, which binds to target genes in combination with beta-catenin (Sabbagh et al., 2018)."
37318981
reach
"The notion that transcriptionally active beta-catenin and LEF -1 complexes were formed in estradiol treated FosER cells was supported by our observation that LEF-1 and TCF-dependent luciferase reporter activity was clearly elevated in the mesenchymal cells."
10629227
sparser
"In the nucleus, β-catenin interacts with members of the lymphoid enhancer factor/T cell factor (LEF/TCF) protein family () and also with other transcription factors, such as Forkhead box protein O1 (F[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24044891
sparser
"Binding of WNT ligands to their receptor stabilizes β-catenin (encoded by CTNNB1 ), which binds to the transcription factors of the lymphoid enhancer-binding factor (LEF) transcription factor (TCF) family."
21472002
sparser
"During gastrulation, β-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 ( xref ) and induce EMT ( xref )."
25249658
sparser
"As discussed above, elevated cytosolic β-catenin levels are associated with increased β-catenin nuclear accumulation and LEF-1-mediated transcription, hence the implication is that presenilin-mediated[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12527355
sparser
"Nuclear β-catenin interacts with members of the Lymphoid Enhancer Factor/T-cell factor (LEF/TCF) family of transcription factors and also with Legless family docking proteins BCL9/BCL9L, which associate with the transcription co-activator pygopus (PYGO) 1/2 ( xref )."
20200414
reach
"These data suggest that Lef1 requires the HMG domain to activate the Col11a1 promoter, either through its DNA binding domain or through interaction with other proteins.In many systems, Lef1 interactio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18280717
biogrid
No evidence text available
10958684
reach
"During gastrulation, beta-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 (XREF_FIG) and induce EMT."
25249658
reach
"Thus, activation of FosER and integrin linked kinase might trigger signaling pathways that are linked to the wnt-pathway or regulate the formation of an active nuclear beta-catenin and LEF -1 complex via as yet unknown mechanisms."
10629227
reach
"To determine whether the in silico identified LEF-1 and TCF binding sequences physically bind to the beta-catenin and Lef -1 complex in vitro, we conducted a competitive electrophoretic mobility-shift assay (EMSA) with a radioactively labeled consensus LEF-1 and TCF strong binding probe and duplex oligonucleotides covering the potential sites in target promoters."
21437251
sparser
"Nuclear β-catenin can interact with T-cell factors (TCFs) and Lymphoid enhancer-binding factors (LEFs) to mediate transcriptional programs mainly involved in cell growth and stemness maintenance, and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682760
sparser
"As transcriptional activity of Lef1 is controlled by recruitment of its co-activator β-catenin, we tested whether Lef1 and β-catenin can form a ternary complex on the TCF/LEF site ( xref F and 5G)."
26212321
reach
"LEF-1 and beta-catenin complexes bind to the promoter region of the E-cadherin gene in vitro, suggesting that this interaction could regulate E-cadherin transcription."
8892228
sparser
"Β-catenin is associated with the lymphoid enhancer factor/T-cell factor family (TCF), converting them from transcription repressors to activators, thereby activating several Wnt-responsive genes ( xref – xref )."
33574925
reach
"In the nucleus, beta-catenin binds to TCF-4 and LEF-1 to produce a transcription factor complex, which mediates cell proliferation and epithelial-mesenchymal transition (EMT) by influencing genes [XREF_BIBR]."
30867651
reach
"In the nucleus, beta-catenin binds TCF and LEF1 transcription factors and drives the expression of specific target genes that control cellular processes ranging from proliferation to cell differentiation."
25124032
sparser
"Behrens J, von Kries JP, Kuhl M, Bruhn L, Wedlich D, Grosschedl R, Birchmeier W. Functional interaction of beta-catenin with the transcription factor LEF1."
25914082
reach
"We recently identified and characterized a novel Wnt like effect of transforming growth factor-beta (TGF-beta) on beta-catenin, the binding partner of Lef-1."
15736165
trips
"Second, once HDAC1-dependent repression has been overridden, beta-catenin binds LEF1 and the beta-catenin-LEF1 complex is competent to activate the expression of downstream target genes."
10958684
reach
"In this study, we show that the beta-catenin and Lef -1 complex is capable of binding to the Jagged-1 promoter in vitro at positions -1933, -1635 and possibly -1083 relative to the translation start site (XREF_FIG and XREF_SUPPLEMENTARY) making these plausible sites for transcriptional interactions between Jagged-1 and the canonical Wnt pathway."
21437251
reach
"Because proinflammatory cytokine induced cartilage degradation appears to involve WNT and beta-catenin signaling and increased WNT and beta-catenin signaling has been implicated in the initiation and progressive deterioration of cartilage degeneration, we hypothesized that small molecule inhibitors of the interaction between beta-catenin and TCF4 and Lef1 could be used to prevent cytokine induced cartilage degradation."
23965253
reach
"As a result it has been further shown that beta-catenin interacts with the transcription factor LEF1, which is in turn associated with Smad 4, thereby regulating the transcription of TGF-beta target molecules XREF_BIBR."
25036590
biogrid
No evidence text available
11751639
reach
"Other therapeutic strategies include preventing β-catenin–LEF-1 complexes from binding to chromatin which in theory may be possible."
24820952
reach
"In our CUT&RUN datasets, approximately 37% of LEF1 binding and 43% of the LEF1/CTNNB1 binding takes place in cis-regulatory regions that only have suboptimal TCF/LEF motifs."
33852891
reach
"These results are consistent with a scenario in which the loss of Wnt activity in migratory cells drives the dissolution of the LEF1/CTNNB1 complex, leading to the subsequent silencing of the specification GRN."
33852891
biogrid
No evidence text available
16204248
sparser
"This activated intra-nuclear β-catenin will further interact with T cell factor (TCF) and lymphoid enhancer factor (LEF) to form a complex which enhances the transcription efficiency of the PD-L1 gene[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34246086
reach
"Second, once HDAC1 dependent repression has been overridden, beta-catenin binds LEF1 and the beta, catenin, and LEF1 complex is competent to activate the expression of downstream target genes."
10958684
reach
"Beta-catenin synthesized in vitro bound strongly and specifically to GST-LEF1 (XREF_FIG C, upper panel), but GAC63 synthesized in vitro did not (XREF_FIG C, lower panel)."
17344318
reach
"Taken together, these results show that COX-2 has a conserved Lef1-binding site at the 3′ region of the genomic locus and binding of Lef1 with β-catenin to the site is linked to increased IL-1β-stimul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17945188
reach
"However, upon IL-1β stimulation, both Lef1 and β-catenin binding were significantly increased ( Fig. 2 B)."
17945188
reach
"The selection of small artificial inhibitors of the nuclear interaction between β-catenin and LEF-1/TCFs has been possible but often suffers from lack of specificity, due to striking overlap in the bi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24820952
reach
"Insulin and glucose inactivate FoxO1 (that accumulates at the cytoplasm) and induce the replacement of TCF4 after nuclear entry of LEF1/β-catenin which recognizes the same binding site.In vivo binding[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25091498
reach
"Insulin stimulation increased the binding of LEF1 and β-catenin while reducing FoxO1 binding."
25091498
reach
"Nuclear beta-catenin binds to and activates the LEF-1 and TCF transcription factors to induce expression of genes involved in tumorigenicity and invasion."
11535620
reach
"Given the important role LEF-1 in regulation of β-catenin (Section 4.3 ) it would be interesting if the above compounds also inhibit β-catenin–LEF-1 complexes."
24820952
reach
"Upon translocation to the nucleus, beta-catenin interacts with Tcf4 and Lef1 to regulate a wide range of gene expression at the transcriptional level [XREF_BIBR]."
20858269
sparser
"Especially, it is noteworthy to mention that a similar result was reported in CRC, which indicates that SNAIL1 serves to control colorectal cancer cell invasion and proliferation by employing β-cateni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36087740
reach
"Beta-catenin and LEF1 signaling is activated in CLL cells, and inhibition of beta-catenin and LEF1 interaction has been proved to induce apoptosis in CLL cells [XREF_BIBR]."
26950276
sparser
"These data support a role for PITX2 in cell proliferation, migration, and cell division through differential Lef-1 isoform expression and interactions with Lef-1 and beta-catenin."
17785445
sparser
"These results indicated that the interaction of the NH 2 -terminus of LEF-1 with β-catenin leads to transcriptional activation."
24269234
sparser
"We also report that Lef1 could bind to β-catenin directly and translocate it into the nucleus, resulting in the activation of the Wnt signaling pathway."
23630438
sparser
"The peptide BLBD synthesized by Hsieh can competitively bind to β-catenin with LEF-1 to interfere with the β-catenin/LEF-1 pathway, inhibiting growth, invasion, migration, and colony formation of breast cancer cells. xref The WASF3-CYFIP1 activated complex can promote actin cytoskeleton recombination, leading to the invasion and metastasis of cancer cells."
32021452
reach
"Given the oncogenic nature of the LEF-1 feedback loop, therapeutic strategies that block both the interaction between β-catenin and LEF-1 and their association with chromatin could have therapeutic va[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24820952
signor
"Upon wnt activation, cytoplasmic beta-catenin is stabilized and enters the nucleus, where it associates with transcription factors, notably tcf (t cell factor) and lef (lymphoid enhancer-binding factor), to regulate the transcription of target genes. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as lef-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus."
23151663
sparser
"Stabilized β-catenin translocates into the nucleus and interacts with the T-cell specific transcription factor and the lymphoid enhancer-binding factor, and then promotes transcription of Wnt target g[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
31323530
sparser
"AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of the Wnt/β-catenin pathway facilitated the maintenance of glioma stem-like cells and their self-renewal [ xref ]."
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