IndraLab

Statements

EGF phosphorylates PTK2. 92 / 94
| 1 33 58 2
reach
"It was observed that expression of WT PKN1 increased TGFbeta1- and EGF dependent phosphorylation of FAK while KN PKN1 expression decreased this effect (XREF_FIG)."
25120372
reach
"We found that EGF stimulation significantly increased the phosphorylation of Src, FAK, Akt, and ERK1/2 in control cells, but these proteins were not significantly activated in Dsg2 depleted cells."
32989241
reach
"EGF stimulates phosphorylation of FAK in pancreatic acini cells in primary culture [24] ."
15694384
sparser
"Pretreatment with wortmannin inhibited EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation in a concentration-dependent manner ( Fig. 6 ) with a half-maximal inhibition occurring with 100 n[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
reach
"XREF_BIBR Focal adhesion may be regulated by multiple factors, and the results show that PKN1 may interfere with TGFbeta- and EGF dependent phosphorylation of FAK (XREF_FIG)."
25120372
sparser
"Although EGF stimulates signal transduction between EGFR and FAK, no evidence for a direct interaction of these two proteins has been observed and it is unclear as to what factor(s) mediates the complex formation between EGFR and FAK and EGF-induced FAK phosphorylation."
20159552
reach
"In BC, ARF1 was found to be highly expressed and fundamental for epidermal growth factor (EGF)-mediated phosphorylation of focal adhesion kinase (FAK) and Src, regulating BC cell proliferation and adhesion (Schlienger et al., 2015)."
38533085
reach
"Previous studies have demonstrated that EGF could upregulate FAK and AKT phosphorylation in glioblastoma cell lines [ 25 , 26 ]."
33508300
reach
"EGF promoted the phosphorylation level of FAK and AKT, while harmine significantly abolished EGF-induced FAK and AKT phosphorylation in glioblastoma cells ( Fig. 3 A-D )."
33508300
sparser
"It was observed that expression of WT PKN1 increased TGFβ1- and EGF-dependent phosphorylation of FAK while KN PKN1 expression decreased this effect ( xref )."
25120372
sparser
"Long et al. reported that a splicing isoform of SRC-3, SRC-3Δ4 can mediate Epidermal Growth Factor Receptor (EGFR) and Focal Adhesion Kinase (FAK) interaction, promoting EGF-induced FAK and c-Src phosphorylation and breast cancer cell migration ( xref )."
34310341
reach
"In this study, we observed that wild-type PTEN inhibited FAK phosphorylation stimulated by EGF, whereas phosphatase inactive mutant PTEN permitted the induction of FAK phosphorylation."
9832564
reach
"This figure also demonstrates that EGF increases FAK phosphorylation in V7 cells."
21533871
sparser
"Moreover, pretreatment of pancreatic acini with 100 μM LY294002 for 1 h caused a 49±12% and 47±14% decrease in the tyrosine phosphorylation of p125 FAK and paxillin by EGF (10 nM) ( Fig. 8 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"Knockdown of MFI2 suppresses EGF-induced FAK phosphorylation in OSCC cells."
37309001
sparser
"Pretreatment with C3 transferase decreased EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation by 77±13% and 78±35%, respectively ( Fig. 9 , top panel lanes 3,4 and b[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"Pretreatment with cytochalasin D completely inhibited EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation ( Fig. 10 , top panel lanes 3,4 and bottom panel)."
9990300
reach
"EGF enhanced FAK phosphorylation and cell spreading in adhering HaCaT cells with low phosphorylated FAK."
18543351
sparser
"Notably, only phospho-FAK was decreased in MFI2-knockdown cells compared with control cells (Fig.  xref C and D), suggesting that MFI2 is an important mediator for regulating the EGF-induced phosphorylation of FAK in OSCC cell lines."
37309001
trips
"In addition, melittin significantly suppressed the EGF-induced FAK phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway."
24675423
sparser
"In the present study, EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation decreased sharply after 2.5 min, such that at 5 min any increase over the unstimulated control was barely detected."
9990300
sparser
"It was unclear what factor mediates the interaction between EGFR and FAK and FAK phosphorylation induced by EGF."
20159552
sparser
"Pretreatment with cetuximab significantly inhibited EGF-induced phosphorylation of Src, FAK, Akt, and ERK1/2 in control cells, but not in Dsg2-depleted cells."
32989241
sparser
"Moreover, the rapid decrease in the EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation contrasts with previous results showing a sustained increased in p125 FAK and paxillin tyrosine phos[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"The dose–response curves for EGF stimulation of tyrosine phosphorylation of p125 FAK and paxillin were similar to the ability of EGF to cause receptor occupation in rat pancreatic acini [1] , demons[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"This interaction promotes EGF-induced phosphorylation of FAK and c-Src which leads to increased cell migration."
37711895
sparser
"The above results suggested that EGF induced the phosphorylation of FAK (397 sites) in colorectal cancer cells, and the the downregulated expression or activity of FAK significantly inhibited EGF-induced EMT in colorectal cancer cells."
32148496
reach
"Interestingly, EGF treatment induced FAK phosphorylation at a different location (Try925) without affecting the phosphorylation status of FAK Tyr397 ( Figure S5 F), as reported previously ( Flinder et[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30612941
sparser
"Moreover, the results of this study showed that EGF induced FAK phosphorylation in Caco-2 cancer cells, and FAK inhibitors could inhibit EGF-induced FAK phosphorylation in colorectal cancer."
32148496
sparser
"Subsequently, in the present study, the effects of shikonin on EGF-induced FAK, Akt and GSK3β phosphorylation were explored."
35949620
sparser
"As demonstrated in xref , EGF upregulated the phosphorylation of FAK, AKT and GSK3β, while shikonin pre-treatment significantly blocked the EGF-induced FAK, AKT and GSK3β phosphorylation ( xref )."
35949620
reach
"Our data showed that prolonged EGF/EGFR signalling activation led to FAK phosphorylation and cytoskeletal re-organization, which further influenced the colony formation and invasion of HNSCC cells tra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27424177
sparser
"Importantly, knockdown of SRC-3Δ4 in MDA-MB231 cells hindered EGF-induced interaction between EGFR and FAK ( xref ), and greatly decreased EGF-stimulated FAK phosphorylation on Y925 ( xref )."
20159552
sparser
"This was established by demonstrating that, after pretreatment of pancreatic acini with thapsigargin in a calcium-free medium to deplete the intracellular calcium stores, EGF-stimulated p125 FAK and [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"This result demonstrated that increases in cytosolic calcium from extracellular stores are also not important for stimulation of p125 FAK and paxillin tyrosine phosphorylation by EGF."
9990300
sparser
"Firstly, EGF may also induce FAK and AKT phosphorylation through other signalling cascades that are insensitive to shikonin."
35949620
sparser
"Previous studies have demonstrated that EGF could upregulate FAK and AKT phosphorylation in glioblastoma cell lines [ 25 , 26 ]."
33508300
sparser
"Jeong et al. probed that melittin could constrain EGF-induced MMP-9 expression via blocking the NF- κ B and PI3K/Akt/mTOR signaling pathway and repress EGF-induced FAK phosphorylation through inhibiting the mTOR/p/0S6K/4E-BP1 signaling pathway in breast cancer cells [ xref ]."
29854840
sparser
"Our study next explored the influences of harmine on EGF-enhanced FAK and AKT phosphorylation."
33508300
reach
"As demonstrated in Fig. 3A, EGF upregulated the phosphorylation of FAK, AKT and GSK3β, while shikonin pre-treatment significantly blocked the EGF-induced FAK, AKT and GSK3β phosphorylation (Fig. 3A, B, E and F)."
35949620
sparser
"Moreover, MEL inhibited mTOR/p70S6K/4EBP1 pathway and thereby significantly suppressed the EGF-induced FAK phosphorylation [ 80 ]."
31408722
sparser
"In contrast, both adhesion-dependent FAK autophosphorylation ( xref and xref ,) and EGF-stimulated FAK phosphorylation were partly abolished in the Kindlin-1 knockdown group ( xref and xref )."
28290610
sparser
"EGF could activate FAK and p130cas phosphorylation, while fibronectin could activate ERK general phosphorylation."
24410474
sparser
"EGF promoted the phosphorylation level of FAK and AKT, while harmine significantly abolished EGF-induced FAK and AKT phosphorylation in glioblastoma cells ( Fig. 3 A-D )."
33508300
reach
"Here we identify SRC-3Delta4, a splicing isoform of the SRC-3 oncogene, as a signaling adaptor that links EGFR and FAK and promotes EGF induced phosphorylations of FAK and c-Src."
20159552
sparser
"This conclusion is supported by the fact that pretreatment of pancreatic acini with two structurally unrelated PI3-kinase activity inhibitors, wortmannin and LY294002, caused a significant reduction i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"In the presence of SOCS-3, EGF-induced FAK phosphorylation was impaired, indicating that SOCS-3 expressed in HEK/DOR cells acts as an inhibitor of pp125FAK."
18804531
reach
"Firstly, EGF may also induce FAK and AKT phosphorylation through other signalling cascades that are insensitive to shikonin."
35949620
sparser
"To examine the role of the small GTP-binding protein p21 rho in EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation, we used the Clostridium botulinum C3 transferase which specifically i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"Our study found that harmine significantly inhibited EGF-induced FAK and AKT phosphorylation."
33508300
sparser
"Our results support the conclusion that p21 rho is important for the ability of EGF to stimulate tyrosine phosphorylation in pancreatic acini because C3 transferase pretreatment markedly inhibited EG[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"As expected and similar to the effect of SRC-3Δ4 knockdown ( xref ), expression of PAK1KD greatly decreased EGF-stimulated FAK phosphorylation at Y925 ( xref )."
20159552
sparser
"In the present study we found that cytochalasin D, which disrupts the integrity of the actin cytoskeleton [38] , completely inhibited EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"Thus, in rat pancreatic acini the phosphorylation of p125 FAK and paxillin by EGF depends on the integrity of the actin cytoskeleton."
9990300
sparser
"In contrast to the action of a number of neuropeptides in other cell systems, EGF-induced p125 FAK and paxillin tyrosine phosphorylation occurs through a PKC- and calcium-independent mechanism."
9990300
reach
"As expected, EGF treatment for 0.5 h induced the phosphorylation of FAK and Akt in both control and MFI2-knockdown SCC4 and KOSC3 cells."
37309001
reach
"SRC-3Delta4 mediates the interaction between EGFR and FAK and augments EGF stimulated FAK and c-Src kinase phosphorylations."
20159552
reach
"It was unclear what factor mediates the interaction between EGFR and FAK and FAK phosphorylation induced by EGF."
20159552
reach
"Thus, in rat pancreatic acini the phosphorylation of p125 FAK and paxillin by EGF depends on the integrity of the actin cytoskeleton.In summary, we found that EGF causes a rapid, concentration depende[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"It was unclear what factor mediates the interaction between EGFR and FAK and EGF-induced FAK phosphorylation."
20159552
sparser
"In addition, MEL significantly suppressed the EGF-induced FAK phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway [ xref ]."
28536009
sparser
"They also stated that MEL significantly suppressed the EGF-induced FAK phosphorylation through inhibition of mTOR/p70S6K/4E–BP1 pathway."
28536009
sparser
"However, the effect of SPARC on FBS- or EGF-induced FAK and Src phosphorylation was less pronounced (data not shown)."
17322388
sparser
"The inhibitory effect of SPARC on EGF-induced phosphorylation of FAK and Src was more pronounced in SKOV3 (Figure 6A) compared with OVCAR3 and IGROV1 cells (data not shown)."
17322388
reach
"In addition, FAK phosphorylation in response to EGF varies depending on the tissue type, with some tissues showing no change [21], neither a decrease [40] nor an increase [41,42] in FAK phosphorylatio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16945503
reach
"Additionally, bee venom suppresses EGF-induced EMT in A549 cells by inhibiting the phosphorylation of ERK, FAK and mTOR and consequently reducing the expression of ZEB2 and Slug [30]."
39001509
reach
"Our results revealed reduced expression of paxillin and decreased phosphorylation of both paxillin and FAK following EGF stimuli in fascin silenced cells."
29088820
reach
"EGFR and FAK phosphorylation was also significantly increased by EGF treatment."
29325228
sparser
"Our finding that EGF stimulates the phosphorylation of FAK in MiaPaCa-2 cells is in agreement with other study reported by Hsia et al. [15] ."
15135307
reach
"EGF could activate FAK and p130cas phosphorylation, while fibronectin could activate ERK general phosphorylation.The effect of EGFR mAbs and TKIs on gastric cancer cells Cetuximab, an IgG1 anti-EGFR mAb, can bind specially to the extracellular domain of EGFR on both normal and tumor cells, thus competitively inhibiting the binding of EGF [36]."
24410474
sparser
"EGF enhanced FAK phosphorylation and cell spreading in adhering HaCaT cells with low-phosphorylated FAK."
18543351
reach
"A recent study identified a splicing isoform of the SRC-3 oncogene, SRC-3Delta4, as a signaling adaptor that links epidermal growth factor receptor (EGFR) and FAK and promotes EGF induced phosphorylations of FAK and c-Src in the signaling cascade leading to epidermal growth factor (EGF)-induced cell migration, an event that requires mechanical signal co-ordination by FA."
21741394
sparser
"In contrast, EGF or PDGF did not stimulate the tyrosine phosphorylation of FAK."
8062907
sparser
"Similarly, while EGF treatment of DAOY cells resulted in increased FAK phosphorylation, ouabain treatment reduced EGF-induced FAK phosphorylation (Fig. xref C) and prevented the formation of EGF-induced actin stress fibers (Fig. xref B)."
25052069
reach
"Phosphorylation of FAK was stimulated by aFGF or bFGF, but not significantly by EGF or PDGF."
8062907
sparser
"However, ouabain inhibited EGF-induced activation of the Erk1/2 and Akt signaling pathways as well as p21 Ras activation, prevented EGF-induced formation of actin stress fibers, and inhibited EGF-induced FAK phosphorylation and DAOY cell motility, possibly through activation of stress signaling."
25052069
reach
"In contradistinction, apical EGF phosphorylated FAK and MAPKs."
16107595
sparser
"Two recent studies in other tissues [19,20] report that EGF can also stimulate tyrosine phosphorylation of p125 FAK and paxillin, suggesting it could be an important signaling cascade for the EGF r[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
reach
"In addition, exogenous epidermal growth factor (EGF) treatment increased FAK phosphorylation."
23525457
reach
"The above results suggested that EGF induced the phosphorylation of FAK (397 sites) in colorectal cancer cells, and the the downregulated expression or activity of FAK significantly inhibited EGF-induced EMT in colorectal cancer cells.After pretreatment with FAK inhibitor PF-228 (10 μM) for 1 hour, the migration and invasion abilities of EGF-induced colorectal cancer cells was determined by the transwell assay."
32148496
reach
"This study demonstrated that EGF/EGFR can phosphorylate FAK and induce EMT in colorectal cancer cells.As knowledge of miRNAs continues to grow, more than 1,000 miRNAs have been identified in human gene banks."
32148496
reach
"Furthermore, we found that bee venom suppressed the EMT-related transcription factors ZEB2 and Slug by blocking EGF-induced ERK, FAK and mTOR phosphorylation."
31786944
sparser
"Moreover, melittin significantly repressed the EGF-induced FAK phosphorylation through inhibition of the mTOR/p70S6K/4E-BP1 pathway [39] ."
33249209
sparser
"EGF stimulates phosphorylation of FAK in pancreatic acini cells in primary culture [24] ."
15694384
reach
"Mechanistically, PTL blocked the phosphorylation of focal adhesion kinase (FAK) and glycogen synthase kinase-3β (GSK3β) induced by epidermal growth factor (EGF)."
38141074
sparser
"Then, ARF1 promotes the EGF-induced phosphorylation and activation of FAK and Src; subsequently, ARF1-activated Src further phosphorylates FAK."
35805075
reach
"Another finding of our study was the observation that CD73 enhanced invasion and metastasis of HNSCC cells, mediated by activation of EGF and EGFR signaling, and its downstream phosphorylation of FAK."
27557512
sparser
"It is unknown, however, what factor mediates the interaction between EGFR and FAK and leads to EGF-induced FAK phosphorylation."
20159552
sparser
"3.5 EBP50 increases EGF-dependent FAK phosphorylation."
22974528
sparser
"In addition, melittin significantly suppressed the EGF-induced FAK phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway."
24675423
sparser
"However, GF109203X pretreatment did not modify EGF-stimulated p125 FAK and paxillin tyrosine phosphorylation ( Fig. 5 A, lanes 3 and 4) or basal tyrosine phosphorylation in con[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9990300
sparser
"In addition, an EGF-induced phosphorylation of FAK, yet another essential mediator for cancer cell invasiveness, was markedly inhibited by ENMD-1198 (Fig. xref )."
18651980