IndraLab

Statements

UCHL5 binds GST. 4 / 4
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"We carried out in vitro binding assays to confirm that S14 or UIP1 could individually bind UCH37 in vitro under conditions other than that of the yeast two-hybrid system, As shown in Fig. 3A,B [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Only the fragment containing the N-terminal 101 residues of NFRKB (N101) bound selectively to GST-Uch37 ( xref )."
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"Almost no detectable FLAG-S14 was found bound to GST-UCH37 when the molar ratio of 6×His-UIP1:FLAG-S14 was 1:1 (lane 6 of Fig. 4A )."
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"However, FLAG-S14 (even at the 6×His-UIP1:FLAG-S14 molar ratio of 1:6) had no significant effect on the amount of 6×His-UIP1 bound to GST-UCH37 (lane 6 of Fig. 4B )."
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