IndraLab

"10 CDK1 phosphorylates NPM at the beginning of mitosis at Thr199 and Thr234/237, resulting in inhibition of NPM RNA binding activity."

No evidence text available

"NPM1 is phosphorylated at Threonine 199 by the cyclin-dependent kinases CDK1 and CDK2 during the cell cycle [ xref ]."

"However, under the experimental conditions used here, the t199 residue was the most likely candidate to be phosphorylated by cyclin b/cdc2 these results strongly support the concept that the rna binding activity of b23.1 is inactivated by cyclin b/cdc2-mediated phosphorylation."

"We also demonstrated for the first time that PPM1D up-regulated phosphorylation of Thr199 and Ser4 of NPM sequentially via the CDC25C-CDK1-PLK1 signalling cascade thereby modulating nucleolar formation (Fig. 6). While phosphorylation at Thr199 by CDK1 and Ser4 by PLK1 has been reported in separate studies27383940, an association between these sites has not previously been reported."

No evidence text available

"NPM1 is phosphorylated at Threonine 199 by the cyclin dependent kinases CDK1 and CDK2 during the cell cycle [XREF_BIBR]."

"Therefore, premature activation of cdk1 in 14-3-3gamma-knockdown cells causes hyper-phosphorylation of T199 residue of NPM1 and possibly other centrosomal proteins resulting in centrosome amplification."

"We also demonstrated for the first time that PPM1D up-regulated phosphorylation of Thr199 and Ser4 of NPM sequentially via the CDC25C-CDK1-PLK1 signalling cascade thereby modulating nucleolar formation (Fig. 6). While phosphorylation at Thr199 by CDK1 and Ser4 by PLK1 has been reported in separate studies27383940, an association between these sites has not previously been reported. Our results suggested that phosphorylation of Thr199 by CDK1 was important for phosphorylation of Ser4 by PLK1. Phosphorylation at Thr199 by CDK1 was suggested to be involved in dissociation of NPM from the centrosome, leading to centrosome duplication40, and in regulation of RNA binding activity of NPM3839."

No evidence text available

"Thr199 and Ser4 of NPM are phosphorylated by G2/M checkpoint kinase CDK1 XREF_BIBR and polo like kinase PLK1 XREF_BIBR, respectively."

"It is suggested that phosphorylation of Thr199 of NPM by CDK1 enhances phosphorylation of Ser4 of NPM by PLK1."

"In this report, we showed that phosphorylation of NPM by CDK1 including Thr199 induces sequential phosphorylation of Ser4 by PLK1."

"T199 phosphorylation of NPM1 is mediated by the cyclin dependent kinases CDK1 and CDK2, which in turn play a key role in NPM1 dissociation from nucleolar components."

"Immunofluorescence studies using an antibody against phosphorylated Thr199 revealed that B23 is phosphorylated at this CDK1 site at the start of mitosis and is dephosphorylated during anaphase."

"We also demonstrated for the first time that PPM1D up-regulated phosphorylation of Thr199 and Ser4 of NPM sequentially via the CDC25C-CDK1-PLK1 signalling cascade thereby modulating nucleolar formation (Fig. 6). While phosphorylation at Thr199 by CDK1 and Ser4 by PLK1 has been reported in separate studies27383940, an association between these sites has not previously been reported. Our results suggested that phosphorylation of Thr199 by CDK1 was important for phosphorylation of Ser4 by PLK1."