IndraLab

Statements

AKT1S1 binds MTOR. 72 / 76
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"When PRAS40 is bound to mTOR, mTOR activity is reduced; in contrast, when mTOR detaches from PRAS40, mTOR is active ( xref )."
29729422
reach
"Increased p-raptor (S792) or PRAS40 binding to mTOR was inhibited by Compound C (XREF_FIG and XREF_FIG; XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), suggesting that AMPK mediates iron chelation increased p-raptor and PRAS40 binding to mTOR."
32541839
reach
"In human cells, PRAS40 binds to mTOR in an insulin dependent manner and may act as a negative regulator of TORC1 in the absence of insulin."
22068330
sparser
"To verify whether AMPK mediates increased p-raptor (S792) or PRAS40 binding to mTOR, IP against mTOR was performed after cells were treated with CPX or Dp44mT, with/without Compound C pretreatment."
32541839
sparser
"In the presence of EtOH, there was an increased interaction between PRAS40 and mTOR."
20127721
reach
"In the presence of EtOH, there was an increased interaction between PRAS40 and mTOR."
20127721
sparser
"Increased p-raptor (S792) or PRAS40 binding to mTOR was inhibited by Compound C (Figs. xref and xref ), suggesting that AMPK mediates iron chelation-increased p-raptor/PRAS40 binding to mTOR."
32541839
hprd
No evidence text available
17277771
sparser
"Genetic investigation of the factors associated with PRAS40 induction during mTOR inhibition identified amplification of EGFR, MDM2, and PDGFRA as more common in the non-responder subgroup, a finding not predicted from preclinical work."
18215108
trips
"PRAS40 binds the mTOR kinase domain and its interaction with mTOR is induced under conditions that inhibit mTOR signalling, such as nutrient or serum deprivation or mitochondrial metabolic inhibition."
17277771
reach
"Treatment with the iron chelators consistently increased the binding of PRAS40 to mTOR, which was attenuated by FeSO 4 pretreatment in all cases."
32541839
reach
"PRAS40 binds the mTOR kinase domain and its interaction with mTOR is induced under conditions that inhibit mTOR signalling, such as nutrient or serum deprivation or mitochondrial metabolic inhibition."
17277771
sparser
"DEPTOR is unique among the mTOR binding proteins in that it associates with both the mTORC1 and -2, and unlike PRAS40 interacts directly with the mTOR protein."
21487027
reach
"Furthermore, PRAS40 binding to mTOR was strongly reduced when Raptor was knocked down (XREF_FIG)."
18030348
biogrid
No evidence text available
18570873
reach
"Additionally, AKT directly phosphorylates AKT1S1 (PRAS40), and this phosphorylation acts to block the inhibitory binding of AKT1S1 to FRAP1 [XREF_BIBR]."
20604938
biogrid
No evidence text available
17386266
sparser
"This is consistent with reports in which stressors such as amino acid deprivation or the glycolytic inhibitor 2-deoxyglucose increased the PRAS40-mTOR interaction [ xref ]."
20127721
sparser
"Additionally, AKT directly phosphorylates AKT1S1 (PRAS40), and this phosphorylation acts to block the inhibitory binding of AKT1S1 to FRAP1 [ xref ]."
20604938
reach
"MTOR interaction with Raptor, mLST8 and GbetaL, and PRAS40 forms the mTORC1 complex."
25845444
reach
"Mechanistically, miR-96 targeted the 3 '-UTR (3 '-untranslated region) of AKT1S1 mRNA leading to its downregulation and disruption of the AKT1S1 and mTOR complex."
29152153
biogrid
No evidence text available
19446321
reach
"As dephosphorylated PRAS40 is known to inhibit mTORC1 by direct binding [XREF_BIBR], we predicted that PRAS40 could also interact with mTOR in this same subcellular compartment."
29895328
sparser
"As dephosphorylated PRAS40 is known to inhibit mTORC1 by direct binding [ xref ], we predicted that PRAS40 could also interact with mTOR in this same subcellular compartment."
29895328
reach
"The binding of mTOR, PRAS40 and RagC to raptor did not differ for control and septic muscle in the basal condition; however, the Leu induced decrease in PRAS40 * raptor and increase in RagC * raptor seen in control muscle was absent in sepsis."
25218136
biogrid
No evidence text available
18030348
reach
"MTOR and PRAS40 interaction : hypertrophy or proliferation."
24131922
sparser
"Similarly, phosphorylation of p70S6K is also restored by IGF1 stimulation in gefitinib-treated cells, suggesting that the well-known interaction of PRAS40 with mTOR could explain the resistance phenomenon."
32467173
sparser
"Furthermore, PRAS40 binding to mTOR was strongly reduced when Raptor was knocked down ( xref )."
18030348
sparser
"The interaction between mTOR and PRAS40 is disrupted by rapamycin, indicating that FKBP12 may mediate its nascent inhibitory activity [ xref , xref – xref ]."
19963289
reach
"In co-immunoprecipitation assays, antibody against mTOR pulled down PRAS40 and MKP7, while antibody against PRAS40 pulled down MKP7, suggesting the interaction of mTOR and PRAS40 with MKP7."
25077541
sparser
"These results suggest that the effect of iron chelation on the PRAS40-mTOR interaction is consistent, but its effect on the raptor-mTOR interaction is cell line dependent."
32541839
biogrid
No evidence text available
19114562
reach
"We noticed that PRAS40 is a downstream target and negative regulator of mTOR activity, PRAS40 binds the mTOR kinase domain, thus inhibits mTOR activity [51], These findings identify PRAS40 as an impor[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21906675
reach
"Researchers illustrated that under basal conditions PRAS40 binds to mTOR to inactivate it, and that mTOR inactivation is relieved when insulin stimulation activates AKT to phosphorylate PRAS40, thereby initiating release from mTOR."
21742795
sparser
"These results suggest that 14-3-3η may be involved in promoting tumorigenesis in pituitary oncocytoma by interacting with PRAS40 (T246) via the mTOR signaling pathway."
31849836
reach
"MTORC1 is comprised of mTOR, mLST8, and RAPTOR alongside the accessory inhibitory factors PRAS40 and DEPTOR, which bind mTORC1 via RAPTOR and mTOR, respectively (Figure 1)."
36321262
sparser
"PIM1 protein kinase overexpression reduced the association of PRAS40 with mTOR, and increased the mTOR directed phosphorylation of 4EBP1 and p70S6Kinase."
19276681
sparser
"Indeed, high levels of pPRAS40 have been associated with high mTOR activity [ xref , xref ]."
27412291
sparser
"To our surprise, the binding of PRAS40 to mTOR was reduced even though PRAS40 phosphorylation on Thr246 was ablated in response to cAMP ( xref A)."
21763421
sparser
"The interaction of PRAS40 with the mTOR complex 1 (mTORC1) inhibits the activity of mTORC1."
19480563
sparser
"The dephosphorylated mTOR and PRAS40 formed a complex resulting in quiescent mTOR activity."
25077541
reach
"The PRAS40-beta5i interaction still occurred in the presence of Triton X100 (XREF_FIG), the detergent that disrupts the PRAS40-mTOR and raptor interaction, indicating that PRAS40 can bind to beta5i without mTOR and raptor."
26876939
reach
"To verify whether AMPK mediates increased p-raptor (S792) or PRAS40 binding to mTOR, IP against mTOR was performed after cells were treated with CPX or Dp44mT, with and without Compound C pretreatment."
32541839
reach
"A newly resolved crystal structure of Arabidopsis mTORC1 shows direct binding of PRAS40 to mTOR at multiple residues including the rapamycin binding pocket [XREF_BIBR]."
29854299
reach
"To our surprise, the binding of PRAS40 to mTOR was reduced even though PRAS40 phosphorylation on Thr246 was ablated in response to cAMP (XREF_FIG A)."
21763421
sparser
"These results suggest that MKP7 could directly dephosphorylate pmTOR and pPRAS40 and forming complexes with these two proteins ( xref )."
25077541
reach
"The interaction between mTOR and PRAS40 is disrupted by rapamycin, indicating that FKBP12 may mediate its nascent inhibitory activity [XREF_BIBR, XREF_BIBR - XREF_BIBR]."
19963289
biogrid
No evidence text available
17277771
reach
"XREF_FIG and S5B, C), suggesting that AMPK mediates iron chelation increased p-raptor and PRAS40 binding to mTOR."
32541839
sparser
"When PRAS40 is bound to mTOR, mTOR activity is reduced; in contrast, when mTOR detaches from PRAS40, mTOR is active ( Saxton and Sabatini, 2017 )."
29729422
reach
"In this case the inhibtion of phosphorylation leads to release of 14-3-3 proteins, inducing binding of AKT1S1 to mTOR and inhibition of function."
32409632
sparser
"Treatment with the iron chelators consistently increased the binding of PRAS40 to mTOR, which was attenuated by FeSO 4 pretreatment in all cases ( xref – xref )."
32541839
sparser
"A newly resolved crystal structure of Arabidopsis mTORC1 shows direct binding of PRAS40 to mTOR at multiple residues including the rapamycin-binding pocket [ xref ]."
29854299
reach
"MTOR is found in two separate multiprotein complexes : mTOR complex (mTORC) 1, in which mTOR interacts with raptor, mLST8, and PRAS40; and mTORC2, formed by mTOR interaction with rictor, mLST8, and mSin."
20332342
biogrid
No evidence text available
18030348
sparser
"AMPK phosphorylation of Raptor is thought to lead to changes in the availability of 14-3-3 for PRAS40 binding, which acts to suppress mTOR kinase activity, thus leading to autophagy initiation."
24710529
sparser
"In addition, 14-3-3 proteins may associate with Raptor and ULK1 under conditions of nutrient deprivation and phosphorylation-dependent 14-3-3 association with Raptor may reduce the availability of 14-3-3 for PRAS40 binding, which may have the combined effect of suppressing mTOR kinase activity and inducing autophagy."
24710529
reach
"When PRAS40 is bound to mTOR, mTOR activity is reduced; in contrast, when mTOR detaches from PRAS40, mTOR is active (Saxton and Sabatini, 2017)."
29729422
reach
"Treatment with the iron chelators consistently increased the binding of PRAS40 to mTOR, which was attenuated by FeSO 4 pretreatment in all cases (XREF_FIG - XREF_FIG)."
32541839
reach
"Increased p-raptor (S792) or PRAS40 binding to mTOR was inhibited by Compound C (Figs."
32541839
reach
"The difference between Torin1 and rapamycin could also be due to the effect of rapamycin in perturbing the interaction between PRAS40 and mTOR and raptor, as revealed previously."
26876939
sparser
"Three main signaling axes were identified for the downregulated phospho-proteins, namely, CHK2-TP53, PRAS40-MTOR and SRC family kinases ( xref B)."
33318047
sparser
"PRAS40 dephosphorylation is associated with the inhibition of mTOR activity in renal cancer cells [ xref ]."
28978182
reach
"Unlike DEPTOR, which inhibits both mTORC1 and mTORC2, PRAS40 (proline rich Akt substrate 40 kDa) binds the mTOR kinase domain in a phosphorylation dependent manner and only suppresses the kinase activity of mTORC1 [XREF_BIBR, XREF_BIBR]."
25450580
sparser
"Increased p-raptor (S792) or PRAS40 binding to mTOR was inhibited by Compound C ( xref and xref ; xref and xref ), suggesting that AMPK mediates iron chelation-increased p-raptor/PRAS40 binding to mTOR."
32541839
biogrid
No evidence text available
19446321
reach
"We also present crystal structures of RAPTOR-TOS motif complexes that define the determinants of TOS recognition, of an mTOR FKBP12-rapamycin-binding (FRB) domain and substrate complex that establishes a second substrate-recruitment mechanism, and of a truncated mTOR and PRAS40 complex that reveals PRAS40 inhibits both substrate-recruitment sites."
29236692
reach
"AKT1S1 (PRAS40) binds the mTOR kinase domain and inhibits mTORC1 mediated signalling."
32409632
biogrid
No evidence text available
18030348
sparser
"In this case the inhibtion of phosphorylation leads to release of 14-3-3 proteins, inducing binding of AKT1S1 to mTOR and inhibition of function."
32409632
sparser
"Treatment with the iron chelators consistently increased the binding of PRAS40 to mTOR, which was attenuated by FeSO 4 pretreatment in all cases (Fig. xref )."
32541839