IndraLab

Statements

UBE2D2 binds OTUB1. 21 / 22
8 | 9 4
reach
"Addition of FAT10 also led to an increased interaction between OTUB1 and its cognate E2 UbcH5B implying a function of FAT10 in the inhibition of polyubiquitylation."
30718280
sparser
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
sparser
"Binding of UBE2D2 to OTUB1, however, lowers the K M for of OTUB1 K48 chains by more than an order of magnitude to within range of physiological substrate concentrations xref ."
32049508
reach
"Resolution was extended by refinement at 3.3 A resolution against the P2 1 X-ray data set to an R factor / R free of 22.1/28.8%, with a doubling of the contents of the asymmetric unit The fold architectures of UbcH5b, OTUB1 and Ub have been described previously and do not deviate significantly in the Ub ~ UbcH5b and OTUB1-Ub complex (see XREF_SUPPLEMENTARY for sequence alignments and secondary structure assignments)."
22325355
biogrid
No evidence text available
28514442
reach
"To assess if free Ub could influence binding of OTUB1 to UbcH5b ~ Ub, we employed a time resolved (TR)-FRET-based assay where UbcH5b ~ Ub was labelled with Tb 3+ cryptate and OTUB1 was labelled with Alexa 488."
22325355
reach
"A near-complete model of the Ub ~ UbcH5b and OTUB1-Ub complex was refined at 3.8 A resolution to an R factor / R free of 21.7/27.9% with good geometry statistics (XREF_TABLE)."
22325355
sparser
"OTUB1 residue Glu28 is sandwiched between UBCH5B residues Lys66 and Arg90 and OTUB1-Asp35 hydrogen bonds with UBCH5B-Lys63, while OTUB1-Leu24 forms hydrophobic interactions with UBCH5B-Leu 119 ( xref )."
23955022
reach
"Compared with a previously published Ub ~ UbcH5b and OTUB1-Ub complex structure, Ubv.B1.1 is rotated by 22degrees relative to the distal site Ub.wt (XREF_FIG)."
23287719
reach
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
biogrid
No evidence text available
22325355
biogrid
No evidence text available
23287719
reach
"Binding of UBE2D2 to OTUB1, however, lowers the K M for of OTUB1 K48 chains by more than an order of magnitude to within range of physiological substrate concentrations 37."
32049508
reach
"This affords van der Waals and salt bridge (between Glu51 in donor Ub and Arg72 and Arg74 in free Ub) interactions between Ub molecules (XREF_FIG), which we suspected could confer interdependency of Ub ~ UbcH5b and Ub binding to OTUB1."
22325355
reach
"A detailed description of the binary interactions defining the overall topology of the Ub ~ UbcH5b and OTUB1-Ub complex follows."
22325355
sparser
"Addition of FAT10 also led to an increased interaction between OTUB1 and its cognate E2 UbcH5B implying a function of FAT10 in the inhibition of polyubiquitylation."
30718280
biogrid
No evidence text available
26186194
biogrid
No evidence text available
26752685
biogrid
No evidence text available
19615732
biogrid
No evidence text available
20725033
biogrid
No evidence text available
22325355