IndraLab
Statements
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"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig. xref ), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."
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"It has been proposed that three pools of cGAS exist in uninfected cells: unmodified, Lys217-sumoylated and constitutively Lys271-ubiquitinated cGAS. xref Sumoylation at Lys217 antagonizes K48-linked ubiquitination of Lys271 of cGAS, thereby inhibiting its proteasomal degradation. xref In our study, we found that USP29 constitutively interacted with cGAS and USP29 deficiency substantially potentiated basal K48-linked ubiquitination of cGAS and downregulated cGAS protein level without affecting its mRNA expression in uninfected cells, indicating that USP29 constitutively removes K48-linked ubiquitination of cGAS to maintain a relatively high level of cGAS under steady conditions."
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"USP29 deubiquitinates cGAS in the presence or absence of HSV-1 infection Because USP29 interacts with cGAS and its enzyme activity is required for regulating antiviral signaling, we investigated b ELISA analysis of IFN-β, IFN-α, TNF, and CCL5 in the supernatants of Usp29 +/+ and Usp29 m/m BMDMs infected with HSV-1 for 0-24 h, or mock transfected (Mock) or transfected with DNA90, HSV60 or HSV120 for 12 h."
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"Results from endogenous immunoprecipitation assays suggested that USP29 constitutively interacted with cGAS in bone marrow-derived macrophages (BMDMs), bone marrow-derived dendritic cells (BMDCs), mouse embryonic fibroblasts (MEFs), mouse lung fibroblasts (MLFs) or human monocytic THP-1 cells and their association was potentiated after HSV-1 infection (Fig. 1b and Supplementary information, Fig. S2e)."
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"Results from endogenous immunoprecipitation assays suggested that USP29 constitutively interacted with cGAS in bone marrow-derived macrophages (BMDMs), bone marrow-derived dendritic cells (BMDCs), mouse embryonic fibroblasts (MEFs), mouse lung fibroblasts (MLFs) or human monocytic THP-1 cells and their association was potentiated after HSV-1 infection (Fig. xref and Supplementary information, Fig. xref )."
sparser
"Interestingly, we found that TRIM38 constitutively interacts with cGAS but not with USP29, whereas USP29 constitutively interacts with cGAS but not with TRIM38 (Supplementary information, Fig. S2h), indicating that TRIM38 and USP29 independently interacts with and regulates cGAS."