IndraLab
Statements
sparser
"To determine which binding pocket was used to mediate the interactions with USP11, PPM1G, DHX40, DDX24 and TRIP12, we repeated the myc-USP7 co-IP experiments in CNE2Z cells, using USP7 mutants with D164A,W165A mutations to disrupt the TRAF binding pocket (referred to as DW) or D762R,D764R mutations to disrupt the Ubl2 pocket (referred to as Ubl2)."
sparser
"Comparison of protein recoveries with individual DW and Ubl2 USP7 mutants showed that interactions with PPM1G, USP11, DDX24 and TRIP12 were greatly affected by the DW mutation and much less affected by the Ubl2 mutation, indicating that these proteins all interact predominantly bind USP7 through the TRAF binding pocket."