IndraLab

Statements

AKT phosphorylates NPM1 on S48. 20 / 20
| 1 13 6
sparser
"We find that AKT phosphorylation of NPM-Ser48 prevents oligomerization that results in nucleoplasmic localization of ARF, constitutive MDM2 inhibition and stabilization of p53."
25071014
sparser
"We find that AKT phosphorylation of NPM-Ser48 inhibits NPM oligomerisation and localization at the nucleolus."
25071014
sparser
"AKT phosphorylation of NPM-Ser48 regulates NPM oligomerization."
25071014
sparser
"We confirmed that AKT specifically phosphorylated NPM on Ser48 by in-vitro kinase assay (Fig. xref )."
25071014
sparser
"Phosphorylated NPM, but not a NPM-S48A derivative, can be detected with a specific phospho-peptide antibody (pS48-NPM) (Fig. xref and xref ) and furthermore, NPM-Ser48 could be phosphorylated by AKT in response to EGF stimulation ( xref )."
25071014
sparser
"Moreover, site directed mutants of NPM that restrict oligomerization also perturb ARF association and nucleolar targeting [ xref , xref , xref ], suggesting that phosphorylation of NPM-Ser48 by AKT may also impact ARF localization and stability."
25071014
sparser
"Taken together these results suggest that nucleolar localization of both ARF and NPM are disrupted by AKT phosphorylation of NPM-Ser48, but that a stable pool of NPM persists in oligomeric form."
25071014
sparser
"The data presented above argues that phosphorylation of NPM-Ser48 by AKT promotes the nucleoplasmic localization of ARF."
25071014
sparser
"The NPM/ARF interaction can be disrupted by DNA damage as well as phosphorylation of NPM at Ser48 by Akt, both of which lead to ARF’s nucleoplasmic transition and interaction with MDM2. xref , xref Following DNA damage, cJun can interact with NPM and cause NPM and ARF redistribution, an event that requires JunB, JNK activation and its phosphorylation of cJun at Thr91 and Thr93. xref "
25908633
sparser
"NPM1 phosphorylation at Ser48 by prosurvival kinase Akt led to NPM1 pentamer disassembly and exit from the nucleolus 7 , and so not surprisingly NPM1 phosphorylated at Ser48 is prevalent in human tumo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38702038
sparser
"Having established that the phosphorylation of NPM-Ser48 by AKT promotes ARF nucleoplasmic localization, MDM2 inhibition and the stabilization of p53 mut , we next wished to address if phosphorylation of NPM-Ser48 was a common phenomenon, and potentially contributing to the stabilization of p53 mut in human tumors."
25071014
rlimsp
"Phosphorylated NPM, but not a NPM-S48A derivative, can be detected with a specific phospho-peptide antibody (pS48-NPM) (Fig. 1D and S1D) and furthermore, NPM-Ser48 could be phosphorylated by AKT in response to EGF stimulation (Fig."
25071014
rlimsp
"We confirmed that AKT specifically phosphorylated NPM on Ser48 by in-vitro kinase assay (Fig. 1C). Phosphorylated NPM, but not a NPM-S48A derivative, can be detected with a specific phospho-peptide antibody (pS48-NPM) (Fig. 1D and S1D) and furthermore, NPM-Ser48 could be phosphorylated by AKT in response to EGF stimulation (Fig."
25071014
rlimsp
"We confirmed that AKT specifically phosphorylated NPM on Ser48 by in-vitro kinase assay (Fig. 1C)."
25071014
reach
"We confirmed that AKT specifically phosphorylated NPM on Ser48 by in-vitro kinase assay."
25071014
sparser
"According to Hamilton, AKT phosphorylates NPM1 on serine 48 to regulate the localization of ARF in PDAC cells [ xref ]."
36428674
rlimsp
"Taken together these results suggest that nucleolar localization of both ARF and NPM are disrupted by AKT phosphorylation of NPM-Ser48, but that a stable pool of NPM persists in oligomeric form."
25071014
rlimsp
"Moreover, site directed mutants of NPM that restrict oligomerization also perturb ARF association and nucleolar targeting [39, 52, 56], suggesting that phosphorylation of NPM-Ser48 by AKT may also impact ARF localization and stability."
25071014
rlimsp
"AKT Phosphorylates NPM on Serine 48 and Regulates NPM Quaternary Structure (A) AKT or IgG immunoprecipitates and whole cell lysates from T24 cells were probed with indicated antibodies."
25071014
sparser
"NPM1 phosphorylation at Ser48 by prosurvival kinase Akt led to NPM1 pentamer disassembly and exit from the nucleolus [ xref ], and so not surprisingly NPM1 phosphorylated at Ser48 is prevalent in human tumors [ xref ]."
38405961