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OTUD6B binds AS1. 399 / 399
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"Previous studies have reported that elevated expression of MRCCAT1, ATB, and SNHG14 in KIRC were correlated with poor prognosis, and this is also the case for low expression of OTUD6B-AS1 and ADAMTS9-AS2."
32974157
sparser
"The correlations between the OTUD6B-AS1 expression level and the clinicopathological features were evaluated using the Mann-Whitney test."
32256450
sparser
"Overall, OTUD6B-AS1 inhibits cellular development in CRC by sponging miR-21-5p and upregulating PNRC2, providing a novel insight into the exploration on CRC treatment."
33686892
sparser
"As mentioned above, we explored the mechanism by which OTUD6B-AS1 overexpression affects ccRCC by performing experiments to demonstrate that OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins in ccRCC cells."
30670025
sparser
"As predicted by TCGA, OTUD6B-AS1 expression was markedly downregulated in CRC tissues compared with in normal tissues ( xref )."
33574932
sparser
"lncRNA OTUD6B-AS1 Downregulates IDH2 Expression by Stabilizing miR-6734-5p."
32952848
sparser
"We next characterized the interactions between lncRNA OTUD6B-AS1 and miR-6734-5p in evoking oxidative damage."
32952848
sparser
"Notably, OTUD6B-AS1 was identified as a molecular sponge of microRNA-664b-3p (miR-664b-3p)."
32682012
sparser
"Furthermore, miR-6734-5p can also inhibit development of colon cancer and acute myeloid leukemia [ xref , xref ]; however, the oxidative damage effects of lncRNA OTUD6B-AS1 and miR-6734-5p against bladder cancer have not previously been reported."
32952848
sparser
"Then, we included the variables age, gender, clinical tumor grade, pathologic stage, lymph node status, metastasis status, and OTUD6B-AS1 mRNA level in a multivariate Cox regression model and found that the OTUD6B-AS1 mRNA level was an independent predictor for overall survival status in KIRC patients (HR = 0.275, 95% CI 0.081–0.930, p  = 0.038; Table  xref )."
30670025
sparser
"Mechanistically, As 2 O 3 increased levels of metal-regulatory transcription factor 1 (MTF1), which regulates lncRNA OTUD6B-AS1, in response to oxidative stress."
32952848
sparser
"These data suggest that OTUD6B-AS1 overexpression can promote G1/G0 arrest, inhibit G2/M phase and increase the apoptotic rate of ccRCC cells in vitro."
30670025
sparser
"Following the treatment of ACHN and OS-RC-2 cells with 5-Aza-CdR, the expression level of OTUD6B-AS1 was significantly higher in the 5-Aza-CdR-treated cells than in the control cells (Fig. xref b)."
30670025
sparser
"The result also showed that OTUD6B-AS1 inhibits the viability, migration, and invasion of thyroid carcinomas by targeting oncogenic miR-183-5p."
32256450
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"Ovarian tumour domain containing 6B antisense RNA1 (OTUD6B-AS1), a newly identified long noncoding RNA (lncRNA), has been reported as a key cancer-related lncRNA."
32682012
sparser
"Twelve differentially expressed lncRNAs ( AL136531.1, MAPT-AS1, AL606834.2, ST7-AS1, AC024361.1, SEMA3B-AS1, AL136368.1, AC004585.1, LINC01871, AL136295.7, LINC01235 , and OTUD6B-AS1 ) were selected as independent prognostic factors (Table xref )."
33947836
sparser
"Collectively, our results reveal that As 2 O 3 provokes oxidative stress by upregulating lncRNA OTUD6B-AS1, which downregulates mitochondrial NADP + -dependent isocitrate dehydrogenase 2 (IDH2) by stabilizing its negative regulator, miR-6734-5p."
32952848
sparser
"In addition, we explored the expression of Nrf2 and found that Nrf2 levels in cytoplasm were clearly elevated following lncRNA OTUD6B-AS1 overexpression or treatment with As 2 O 3 ; however, Nrf2 in the nucleus showed the opposite trend (Figures xref – xref )."
32952848
sparser
"Overexpression of OTUD6B-AS1 markedly suppressed the proliferation of ccRCC cells in vitro."
30670025
sparser
"OTUD6B-AS1 inhibits tumorigenesis in ccRCC cells in vivo."
30670025
sparser
"These findings indicate that increased lncRNA OTUD6B-AS1 may be able to inhibit bladder cancer development, which is similar to the findings of Wang et al. [ xref ] that lncRNA OTUD6B-AS1 can suppress progression of clear cell renal cell carcinoma."
32952848
sparser
"The qRT-PCR results showed that OTUD6B-AS1 expression was significantly enhanced in the SW579 and TPC-1 cells after transfection with OTUD6B-AS1- pcDNA3.1 compared to transfection with empty pcDNA3.1 ( xref )."
32256450
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"As 2 O 3 Upregulates lncRNA OTUD6B-AS1 Expression via MTF1."
32952848
sparser
"lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is oriented in an antisense direction relative to the protein-coding gene OTUD6B on the opposite DNA strand."
32256450
sparser
"To test the OTUD6B-AS1 expression levels in ccRCC cells, we performed qRT-PCR assays and found that the expression levels of OTUD6B-AS1 were downregulated in the ccRCC cell lines compared with HK-2 cells."
30670025
sparser
"Overall, these findings indicate that the lncRNA OTUD6B-AS1 accelerates the proliferation and invasion of HCC cells by enhancing GSKIP/Wnt/β-catenin signalling via the sequestration of miR-664b-3p."
32682012
sparser
"These results demonstrate that lncRNA OTUD6B-AS1 functions to increase the miR-6734-5p level by enhancing miR-6734-5p stability."
32952848
sparser
"Online programs predicted miR-183-5p and miR-21 as potential targets of OTUD6B-AS1."
32256450
sparser
"The results revealed that miR-6734-5p levels were significantly increased, by more than 5.18-fold, in T24 cells overexpressing lncRNA OTUD6B-AS1 relative to untreated control cells ( P < 0.01) ( xref )."
32952848
sparser
"In addition, we assessed whether MTF1 could activate transcription from the lncRNA OTUD6B-AS1 promoter using a dual-luciferase reporter assay."
32952848
sparser
"Importantly, lncRNA OTUD6B-AS1 dramatically exacerbated As 2 O 3 -induced cytotoxicity by inducing oxidative stress."
32952848
sparser
"Additionally, western blot assays revealed remarkable alterations in CCNB1 and CCND1 expressing in the ACHN and OS-RC-2 cells overexpressing OTUD6B-AS1, confirming that OTUD6B-AS1 is involved in cell cycle regulation (Fig. xref c)."
30670025
sparser
"Therefore, it was hypothesized that OTUD6B-AS1 could inhibit CRC progression via inhibition of miR-3171 expression."
33574932
sparser
"The upregulation of OTUD6B-AS1 expression significantly upregulated the expression of the epithelial marker E-cadherin, while the expression levels of the mesenchymal cell markers N-cadherin and Snail were downregulated in both the ACHN and OS-RC-2 cells with enhanced expression of OTUD6B-AS1 (Fig. xref c)."
30670025
sparser
"BC cases were divided into high-risk and low-risk groups based on the median risk score, calculated by 8 GT-lncRNAs, including MIR4435-2HG, MAPT-AS1, TGFB2-AS1, AL357054.4, AL161719.1, OTUD6B-AS1, AC083799.1, and LINC01016."
36189319
sparser
"In summary, we first investigated the expression pattern of OTUD6B-AS1 in ccRCC tissue and cell lines."
30670025
sparser
"Significantly reduced lncRNA OTUD6B-AS1 levels were observed in MTF1 knockdown cells ( P < 0.01), and the elevated lncRNA OTUD6B-AS1 levels in As 2 O 3 -treated T24 cells were significantly attenuated by MTF1 knockdown ( P < 0.01) ( xref )."
32952848
sparser
"Additionally, further studies are needed to determine whether other As 2 O 3 -induced cytotoxic effects, including inhibition of cancer stem-like cells, are also mediated by lncRNA OTUD6B-AS1."
32952848
sparser
"Likewise, OTUD6B-AS1 was classified as a ferroptosis-related risk lncRNA in BC survival ( xref )."
36189319
sparser
"Furthermore, the overexpression of OTUD6B-AS1 significantly decreased proliferation and tumorigenesis while partly inhibiting cell migration and invasion in ccRCC cells in vitro."
30670025
sparser
"The lncRNA OTUD6B-AS1 (OTUD6B antisense RNA 1) is down-regulated in human RCC tissues, and lower OTUD6B-AS1 expression in RCC tissues correlates with poor patient prognosis [ xref ]."
33593347
sparser
"However, the expression and biological functions of the lncRNA OTUD6B-AS1 are still unknown in tumors, including clear cell renal cell carcinoma (ccRCC)."
30670025
sparser
"Here, we demonstrated that the antioncogenic effect of OTUD6B-AS1 is partly mediated through the inhibition of the activity of the Wnt/β-catenin pathway and the EMT-related pathway."
30670025
sparser
"The result suggested that lower OTUD6B-AS1 expression was closely related to the growth and metastasis of thyroid cancer."
32256450
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"Xenograft tumors with both lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment showed significantly weaker Ki-67 expression ( xref ) and significantly stronger p57 expression ( xref ), compared with xenograft tumors with lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment alone."
32952848
sparser
"The long noncoding RNA OTUD6B-AS1 enhances cell proliferation and the invasion of hepatocellular carcinoma cells through modulating GSKIP/Wnt/β-catenin signalling via the sequestration of miR-664b-3p."
32682012
sparser
"The regulation of lncRNA OTUD6B-AS1 expression by As 2 O 3 requires further exploration in a clinical context, to corroborate our findings."
32952848
sparser
"Furthermore, binding of MTF1 to the lncRNA OTUD6B-AS1 promoter sequence was directly proven by both ChIP and dual-luciferase reporter assays."
32952848
sparser
"Previously, lncRNA OTUD6B-AS1 was reported to have an antioncogenic role in clear cell renal cell carcinoma, associated with the Wnt/ β -catenin signaling pathway [ xref ]."
32952848
sparser
"In tumors, OTUD6B-AS1 regulates proliferation, migration and invasion."
32256450
sparser
"Mechanistically, OTUD6B-AS1 inactivates the Wnt/β-catenin pathway and suppresses the expression of epithelial-to-mesenchymal transition-related proteins, including E-cadherin, N-cadherin and Snail in RCC cells [ xref ]."
33593347
sparser
"Moreover, decreased expression of miR-183-5p and miR-21 was observed in SW579 and TPC-1 cells that overexpressed OTUD6B-AS1 ( xref )."
32256450
sparser
"The lncRNA OTUD6B-AS1 is oriented in an antisense direction to the protein-coding gene OTUD6B on the opposite DNA strand."
30670025
sparser
"In this research, we explored the role and mechanism of lncRNA ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) in CRC."
33686892
sparser
"The overexpression of OTUD6B-AS1 inhibited the proliferation, migration and invasion of ccRCC cells in vitro and in vivo and promoted apoptosis in ccRCC cells."
30670025
sparser
"A total of 14 lncRNAs were identified as protective genes (AC022150.4, AC061992.1, AC090948.3, AC092794.1, AC107464.3, AL021707.8, AL451085.2, AL606834.2, FLJ42351, LINC00926, LINC01871, TNFRSF14-AS1, U73166.1 and USP30-AS1) with HRs < 1 while 10 lncRNAs (AC022150.2, AC090948.1, AC243960.1, AL021707.6, ITGB2-AS1, OTUD6B-AS1, SP2-AS1, TOLLIP-AS1, Z68871.1 and ZNF337-AS1) were associated with increased risk with HRs >1."
35441740
sparser
"Further, lncRNA OTUD6B-AS1 inhibited mitochondrial NADP + -dependent isocitrate dehydrogenase 2 (IDH2) expression by stabilizing miR-6734-5p, which contributed to cytotoxicity by enhancing oxidative stress."
32952848
sparser
"OTUD6B-AS1 Inhibits Migration and Invasion of Thyroid Carcinoma."
32256450
sparser
"To gain insights into the molecular mechanism underlying lncRNA OTUD6B-AS1 exacerbation of As 2 O 3 -induced cytotoxicity, levels of oxidative stress were evaluated in T24 cells."
32952848
sparser
"To further verify whether MTF1 is responsible for As 2 O 3 regulation of lncRNA OTUD6B-AS1 transcription, endogenous MTF1 was knocked down using shRNA ( xref )."
32952848
sparser
"OTUD6B-AS1 and miR-21 constitute a regulatory axis within thyroid carcinomas."
32256450
sparser
"The long noncoding RNA (lncRNA) OTUD6B antisense RNA 1 (OTUD6B-AS1) is oriented in an antisense direction to the protein-coding gene OTUD6B on the opposite DNA strand."
30670025
sparser
"In this work, we investigated the expression of OTUD6B-AS1 in human thyroid carcinoma tissues and corresponding adjacent normal thyroid tissue samples."
32256450
sparser
"Collectively, our results demonstrated that OTUD6B-AS1, acting as an antioncogene, may be a potential diagnostic and prognostic biomarker as well as a therapeutic target in ccRCC."
30670025
sparser
"As 2 O 3 could activate lncRNA OTUD6B-AS1 transcription in bladder cancer cells, and these findings were validated in a xenograft tumor model."
32952848
sparser
"A recent study reveals the role of OTUD6B-AS1 in clear cell renal cell carcinoma (ccRCC) ( xref )."
32256450
sparser
"The potential targets of OTUD6B-AS1 were screened using the online programs OncomiR and StarBase 3.0, and the LncBase Predicted v."
32256450
sparser
"The expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue compared with normal tissue and was associated with clinicopathological features of patients with ccRCC."
30670025
sparser
"As shown in xref , miR-6734-5p was predicted to have three binding sites in lncRNA OTUD6B-AS1."
32952848
sparser
"These data indicated that miR-21 and miR-183-5p are bound to OTUD6B-AS1."
32256450
sparser
"Subsequently, we overexpressed lncRNA OTUD6B-AS1 8.79-fold by transfecting T24 cells with a lentiviral vector ( xref ), to quantitatively evaluate the biological function of lncRNA OTUD6B-AS1 in As 2 O 3 -induced cytotoxicity in vitro ."
32952848
sparser
"On the other hand, Wang et al. demonstrated that the antioncogenic effect of OTUD6B-AS1 is partly mediated through the inhibition of the activity of the Wnt/β-catenin pathway and the EMT-related pathway ( xref )."
32974157
sparser
"Together, these results further indicate that lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced cytotoxicity."
32952848
sparser
"Accordingly, we sought out to determine the mechanism through which MTF1 regulates lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Then, Kaplan-Meier analysis was used to evaluate the relationship between OTUD6B-AS1 expression in ccRCC and patient clinicopathological characteristics, and the results showed that the survival time of the patients with pathologic stage I + II disease ( n  = 318) was longer than that of the patients with advanced stage lesions ( n  = 205) ( P  < 0.0001, Additional file xref : Figure S1A)."
30670025
sparser
"On the contrary, LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were highly expressed in the high-risk groups, demonstrating that these FRLncRNAs were high-risk LncRNAs."
34671639
sparser
"The survival time of the patients with high OTUD6B-AS1 expression ( n  = 26) was longer than that of the patients with low OTUD6B-AS1 expression ( n  = 26) ( P  < 0.0001, Fig. xref e)."
30670025
sparser
"LncRNA OTUD6B-AS1 overexpression promoted GPX4-mediated ferroptosis to suppress radioresistance in colorectal cancer."
37184781
sparser
"Ferroptosis inhibitor attenuated radioresistance (P < 0.01) caused by lncRNA OTUD6B-AS1 overexpression."
37184781
sparser
"These findings were confirmed by the results of ki-67 staining assays (Fig. xref h) and highlighted OTUD6B-AS1 as an antioncogene in ccRCC cells."
30670025
sparser
"Eight of these lncRNAs (OTUD6B-AS1, AL122010.1, AC136475.2, AL161646.1, AC245297.3, LINC00578, LINC01871, AP000442.2) were selected for establishment of the risk prediction model."
33225954
sparser
"The different expression level of OTUD6B-AS1 correlated with tumor size, clinical stage and lymphatic metastasis of thyroid carcinomas."
32256450
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"Western blotting showed that, under lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment (20  μ mol/L), the expression of Bax and Caspase3 was significantly increased, while levels of Bcl-2 were significantly decreased relative to lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment ( P < 0.01; Figures xref – xref )."
32952848
sparser
"Although several publications have indicated the occurrence of necroptosis regulators in KIRC, very few data are available on the involvement of lncRNAs in controlling or decreasing the neurotrophic signaling in kidney cancer, in particular based on OTUD6B-AS1, AL162377.1, AC108449.2, AF111167.2, and hsa-miR-21-5p targeting KLF9 [ xref – xref ]."
36226251
sparser
"As shown in xref , the luciferase activity driven by the WT promoter sequence was markedly reduced by MTF1 knockdown, indicating that the WT sites are required for binding of MTF1 to the lncRNA OTUD6B-AS1 promoter."
32952848
sparser
"OTUD6B-AS1 regulates proliferation and apoptosis via cyclin D1 expression in a sense gene independent manner ( xref )."
32256450
sparser
"Since ceRNAs represent a novel aspect of regulation, especially during tumorigenesis [ xref ], ongoing work is strongly needed to determine whether OTUD6B-AS1 could be a candidate ceRNA during ccRCC progression."
30670025
sparser
"The Wnt/β-catenin pathway is evolutionarily well-conserved, and aberrant activation of this pathway can cause abnormal cell growth and malignant transformation ( xref ; xref ). xref revealed that the novel lncRNA OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
33178684
sparser
"Therefore, it was hypothesized that OTUD6B-AS1 could exert certain effects on the proliferation, invasion and migration of CRC cells by regulating miR-3171."
33574932
sparser
"We speculated that OTUD6B-AS1 affects macrophages and differentiating T cells through miR-21, then affects immune status, and finally affects the occurrence and development of tumor."
33225954
sparser
"LncRNA OTUD6B-AS1 Induces Cisplatin Resistance in Cervical Cancer Cells Through Up-Regulating Cyclin D2 via miR-206."
34746018
sparser
"OTUD6B-AS1 and miR-183-5p, constitute another regulatory axis of thyroid carcinomas."
32256450
sparser
"The lncRNA OTUD6B-AS1 therefore exerts tumor suppressive effects by inactivating the Wnt/β-catenin pathway (Table  xref )."
33593347
sparser
"To evaluate the impact of OTUD6B-AS1 on the ccRCC cell cycle and apoptosis, flow cytometry assays and Hoechst staining analysis were conducted."
30670025
sparser
"To identify the function of OTUD6B-AS1 in ccRCC, we performed gain-of-function assays."
30670025
sparser
"To determine whether OTUD6B-AS1 can exert its effects on proliferation, invasion and migration of HCT116 cells by targeting miR-3171, a series of functional experiments was performed."
33574932
sparser
"Finally, we accordingly constructed a risk prognostic model based on the 6 identified m6A-related lncRNAs, including Z68871.1, AL122010.1, OTUD6B-AS1, AC090948.3, AL138724.1, EGOT."
34722303
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"Furthermore, we found that MTF1 knockdown eliminated the As 2 O 3 -induced increase of lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Luciferase reporter assay was used to confirm the interactions between OTUD6B-AS1 and its potential targets."
32256450
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"LncRNA OTUD6B-AS1 inhibits many cellular processes in colorectal cancer by sponging miR-21-5p and regulating PNRC2."
33686892
sparser
"Luciferase reporter assays showed miR-183-5p and miR-21 bound to OTUD6B-AS1."
32256450
sparser
"Recently, the involvement of dysregulated long noncoding RNA ovarian tumor domain containing 6B antisense RNA1 (lncRNA OTUD6B-AS1) in tumorigenesis is established."
32952848
sparser
"In the current study, we found that the overall survival was lower under high OTUD6B-AS1 expression."
33225954
sparser
"The results of qRT-PCR analyses showed that lncRNA OTUD6B-AS1 levels in T24 bladder cancer cells were significantly and dose-dependently increased in response to As 2 O 3 ; lncRNA OTUD6B-AS1 levels were increased by more than 8.65-fold by As 2 O 3 treatment at 20  μ mol/L for 6 h, relative to untreated T24 cells ( P < 0.01) ( xref )."
32952848
sparser
"Compared to the normal thyroid cells Nthy-ori, decreased expression of OTUD6B-AS1 was observed in the SW579 and TPC-1 cells ( p < 0.001, xref )."
32256450
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"Next, we screened potential targets of OTUD6B-AS1 using several online programs, OncomiR, StarBase 3.0 and LncBase Predicted v."
32256450
sparser
"The Overexpression miR-183-5p and miR-21 Partly Compromised the Inhibitory Effects of OTUD6B-AS1 on Migration and Invasion."
32256450
sparser
"We investigated the possible pathways that contribute to the effects of OTUD6B-AS1 on ccRCC."
30670025
sparser
"Although previous studies have shown that OTUD6B-AS1 expression is deregulated in the skin tissue of patients with systemic sclerosis [ xref ], the mechanism of OTUD6B-AS1 in tumors and other diseases, especially RCC, is unknown."
30670025
sparser
"Intriguingly, gain-of-function experiments demonstrated the effects of the combination of lncRNA OTUD6B-AS1 and miR-6734-5p in promoting oxidative stress, and knockdown of IDH2 also had a similar effect."
32952848
sparser
"Interestingly, following lncRNA OTUD6B-AS1 overexpression, levels of ROS and MDA in T24 cells treated with As 2 O 3 were significantly enhanced ( P < 0.01); however, the activities of the antioxidant enzymes SOD and GSH-Px showed the opposite trend, suggesting an impaired antioxidant defense system (Figures xref and xref )."
32952848
sparser
"Elevated expression of the lncRNA was also found in multiple HCC cell lines and the silencing of OTUD6B-AS1 significantly decreased proliferation, colony formation and invasion."
32682012
sparser
"This result found that miR-21 overexpression promoted the viability, migration, and invasion, reversed OTUD6B-AS1 effect in thyroid carcinomas."
32256450
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"Additionally, compared with OTUD6B-AS1 group, miR-183-5p and miR-21 expression were significantly increased after co-transfected OTUD6B-AS1, miR-183-5p, and (or) miR-21 mimic into SW579 and TPC-1 cells ( xref )."
32256450
sparser
"In the current study, we reported that OTUD6B-AS1 overexpression inhibited cells viability, migration, and invasion, similar with the effect of OTUD6B-AS1 in ccRCC."
32256450
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"OTUD6B-AS1 and LINC00578 were discovered as prognostic signatures in breast cancer for the first time."
33225954
sparser
"The lncRNA OTUD6B-AS1 also regulates the Wnt/β-catenin signaling pathway to induce the proliferation and invasion of hepatocellular carcinoma [ xref ]."
34201062
sparser
"We investigated the expression of OTUD6B-AS1 in 60 pair of thyroid carcinoma tissues and adjacent para-cancerous tissues."
32256450
sparser
"These results suggested that miR-3171 is a direct target of OTUD6B-AS1."
33574932
sparser
"In addition, levels of lncRNA OTUD6B-AS1 in xenograft tumors were confirmed to increase with lncRNA OTUD6B-AS1 overexpression, As 2 O 3 treatment, or both (all P < 0.01; xref )."
32952848
sparser
"However, the expressions and biological functions of OTUD6B-AS1 are still unknown in thyroid carcinomas."
32256450
sparser
"The expression of OTUD6B-AS1 correlated with tumor size, clinical stage, and lymphatic metastasis of thyroid carcinoma."
32256450
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"First, we found that the OTUD6B-AS1 promoter was methylated by consulting the UCSC database ()."
30670025
sparser
"These data suggest that lncRNA OTUD6B-AS1 transcription is regulated by MTF1."
32952848
sparser
"In the field of cancer research, Wang and colleague reported OTUD6B-AS1 was downregulated in ccRCC tissue samples, and that patients with low OTUD6B-AS1 expression had shorter overall survival than patients with high OTUD6B-AS1 expression, which showed that the different expression levels of OTUD6B-AS1 indirectly correlated with patient survival ( xref )."
32256450
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"The dual-luciferase reporter assay demonstrated the binding of miR-3171 and OTUD6B-AS1, since the miR-3171 mimic + OTUD6B-AS1 WT group exhibited lower luciferase activity compared with the mimic-NC + OTUD6B-AS1 WT group ( xref )."
33574932
sparser
"Furthermore, the elevation of Nrf2 in cytoplasm induced by As 2 O 3 was further significantly enhanced by lncRNA OTUD6B-AS1; however, Nrf2 in the nucleus showed the opposite trend."
32952848
sparser
"To our knowledge, no mechanism underlying the regulation of miRNA by lncRNA OTUD6B-AS1 has previously been reported."
32952848
sparser
"Further, lncRNA OTUD6B-AS1 significantly reduced IDH2 levels by 50% ( P < 0.01) (Figures xref and xref )."
32952848
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"As shown in xref , co-transfection of OTUD6B-AS1 overexpression plasmid and miR-3171 mimics reversed the inhibitory effects of OTUD6B-AS1 overexpression alone on the proliferation, colony formation abilities and the Ki67 expression of HCT116 cells."
33574932
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"The overexpression of OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of epithelial-to-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin and Snail) in ccRCC cells."
30670025
sparser
"Data from The Cancer Genome Atlas database reveal that lncRNA OTUD6B-AS1 levels are reduced and that lncRNA OTUD6B-AS1 acts as an antioncogene in various tumors [ xref ]."
32952848
sparser
"Upregulation of OTUD6B-AS1 expression induces ccRCC cell apoptosis and alters cell cycle progression in vitro."
30670025
sparser
"Based on the results above, we can infer that OTUD6B-AS1 can regulate the activity of the Wnt/β-catenin signaling pathway and affect the expression of several EMT-related proteins in ccRCC cells."
30670025
sparser
"As shown in Figures xref – xref , lncRNA OTUD6B-AS1 upregulated ROS and MDA and this activity could be significantly enhanced by miR-6734-5p."
32952848
sparser
"We first examined the expression of OTUD6B-AS1 in thyroid carcinoma cells SW579 and TPC-1, and normal thyroid cells Nthy-ori."
32256450
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"qRT-PCR analysis was performed at 48 h posttransfection, and the data revealed that OTUD6B-AS1 expression was significantly increased by plvx-OTUD6B-AS1 compared with the empty vector. (Fig. xref c)."
30670025
sparser
"The effects of OTUD6B-AS1 on thyroid carcinoma cells were determined via the MTT and transwell assays."
32256450
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"LncRNA OTUD6B-AS1 was downregulated in ccRCC cell lines."
30670025
sparser
"Kaplan-Meier analysis was used to evaluate the relationship between OTUD6B-AS1 expression in ccRCC and patient survival, and the results showed that lower OTUD6B-AS1 expression was associated with poor survival."
30670025
sparser
"OTUD6B-AS1 is transcribed from the opposite strand of the OTUD6B gene, which is located on chromosome 8 in head-to-head orientation to OTUD6BAS1 [ xref ]."
33225954
sparser
"Additionally, the use of only one TCGA dataset to investigate OTUD6B-AS1 expression is another limitation of the present study, and future studies should improve upon this and the other limitations."
33574932
sparser
"To explore the role of the lncRNA OTUD6B-AS1 in ccRCC, we analyzed the expression of OTUD6B-AS1 in a cohort of 75 ccRCC tissue samples and matched nontumor samples using qRT-PCR analysis, with our results showing that OTUD6B-AS1 expression was remarkably decreased in the ccRCC tissue samples compared with the paired adjacent normal tissue samples (Fig.  xref a and b)."
30670025
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"Taken together, these findings support the prediction that miR-21 and miR-183-5p are the direct target of OTUD6B-AS1."
32256450
sparser
"The results demonstrated that OTUD6B-AS1 expression was low in CRC cells and tissues."
33574932
sparser
"In the present study, a binding site between OTUD6B-AS1 and miR-3171 was predicted using the Starbase database, and miR-3171 expression was upregulated in HCT116 cells."
33574932
sparser
"Furthermore, miR-3171 was demonstrated to be a direct target of OTUD6B-AS1 using a luciferase reporter assay."
33574932
sparser
"These findings indicate that OTUD6B-AS1 upregulation suppresses ccRCC tumor cell growth in vivo."
30670025
sparser
"In general, our study found (1) miR-26a-5p was a protective factor of breast cancer, while OTUD6B-AS1 and MTDH were risk factors; (2) OTUD6B-AS1 was the up-stream regulator of miR-26a-5p verified by luciferase; (3) up-regulation of miR-26a-5p and down-regulation of MTDH promoted cellular cytotoxicity of paclitaxel (PTX) in vitro and in vivo . (4) down-regulation of miR-26a-5p, overexpression of MTDH and OTUD6B-AS1 promoted autophagy and DNA damage; (5) up-regulation of OTUD6B-AS1 and MTDH inhibited DNA damage response (DDR) by inhibiting the phosphorylated activation of RAD51, ATR and ATM."
34740994
sparser
"This study was designed to determine the functional significance and regulatory mechanism of OTUD6B-AS1 in HCC."
32682012
sparser
"Conclusions: Taken together, our findings present in vitro evidence of lncRNA OTUD6B-AS1 as a tumor suppressor in thyroid carcinomas."
32256450
sparser
"Cumulatively, we believe that As 2 O 3 -induced cell damage is achieved by upregulation of lncRNA OTUD6B-AS1."
32952848
sparser
"Taken together, our findings present a road map for targeting the newly identified lncRNA OTUD6B-AS1 to suppress ccRCC progression in cell lines, and these results elucidate a novel potential therapeutic target for ccRCC treatment."
30670025
sparser
"These data suggest that As 2 O 3 activates lncRNA OTUD6B-AS1 transcription in bladder cancer."
32952848
sparser
"OTUD6B-AS1 suppresses the proliferation, invasion and migration of HCT116 cells by targeting miR-3171."
33574932
sparser
"OTUD6B-AS1 expression in CRC cell lines was examined using reverse transcription-quantitative PCR."
33574932
sparser
"Functional assays showed that lncRNA OTUD6B-AS1 dramatically exacerbated As 2 O 3 -mediated oxidative damage by inducing oxidative stress."
32952848
sparser
"Furthermore, the expression levels of OTUD6B-AS1 were assessed in different CRC cell lines and in normal HIEC cells."
33574932
sparser
"Furthermore, OTUD6B-AS1 overexpression partly inhibited cell migration and invasion."
30670025
sparser
"These data indicate that lncRNA OTUD6B-AS1 can suppress IDH2 expression through stabilization of miR-6734-5p."
32952848
sparser
"Besides, Kong et al. [ xref ] confirmed in their research that silencing OTUD6B-AS1 obviously reduced the proliferation and invasion of HCC cells."
33604387
sparser
"LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability."
34740994
sparser
"Two miRNAs, miR-183-5p and miR-21, were found to be potential targets of OTUD6B-AS1 ( xref )."
32256450
sparser
"Furthermore, the elevation of Nrf2 in cytoplasm induced by lncRNA OTUD6B-AS1 was further significantly enhanced by miR-6734-5p; however, Nrf2 in the nucleus showed the opposite trend (Figures xref – xref )."
32952848
sparser
"The effects of OTUD6B-AS1 on ccRCC cells were determined via MTT assay, clone formation assay, transwell assay, and flow cytometry."
30670025
sparser
"This study demonstrates that the lower expression of OTUD6B-AS1 is associated with poor prognosis of ccRCC ( xref )."
32256450
sparser
"The sequences of the binding sites of lncRNA OTUD6B-AS1 with miR-21 and miR-183-5p were shown in xref ."
32256450
sparser
"Here, for the first time, we demonstrate that lncRNA OTUD6B-AS1 expression is upregulated by As 2 O 3 and that lncRNA OTUD6B-AS1 in regulated in response to oxidative stress."
32952848
sparser
"The subcellular localization of lncRNA OTUD6B-AS1 was tested by the nuclear/cytoplasmic fractionation assay, with RNA immunoprecipitation assay to validate gene interactions."
37184781
sparser
"Moreover, silencing OTUD6B-AS1 inhibited HCC proliferation and invasion through the Wnt/ β -catenin pathway [ xref ]."
34594378
sparser
"Furthermore, ROS and MDA production also increased significantly in response to lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"Methods: The expression level of OTUD6B-AS1 was measured in 60 paired human thyroid carcinoma tissues and corresponding adjacent normal thyroid tissues."
32256450
sparser
"lncRNA OTUD6B-AS1 Overexpression Inhibits Bladder Cancer Progression."
32952848
sparser
"Currently, no OTUD6B-AS1 that act as ceRNAs have been reported in thyroid carcinomas."
32256450
sparser
"Long non-coding RNA OTUD6B-AS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells via downregulation of microRNA-3171."
33574932
sparser
"LncRNA ENST00000524003 (lncRNA OTUD6B-AS1) was downregulated in renal clear cells and inhibited the migration and invasion of cancer cells by inhibiting the activity of Wnt/ catenin pathway and epithelial-mesenchymal transformation [ xref ]."
33493136
sparser
"Transwell migration and invasion assays showed that SW579 and TPC-1 cells overexpressed OTUD6B-AS1 decreased migration and invasion abilities ( xref )."
32256450
sparser
"The results of the CCK-8 assay ( xref ) showed that both lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment (20  μ mol/L) significantly decreased T24 cell viability to 40% ( P < 0.01)."
32952848
sparser
"Furthermore, The Cancer Genome Atlas database was utilized to examine the expression levels of OTUD6B-AS1 in CRC tissues."
33574932
sparser
"lncRNA OTUD6B-AS1 Exacerbates As"
32952848
sparser
"The results of CCK-8 assays also revealed significantly lower cell viability of bladder cells overexpressing lncRNA OTUD6B-AS1 and miR-6734-5p mimics ( P < 0.01) ( xref )."
32952848
sparser
"Recent accumulating evidence has indicated that lncRNAs, such as OTUD6B-AS1, URRCC, HOTAIRM1 and MRCCAT1, play important regulatory roles in diverse biological processes in ccRCC [ xref – xref ]."
| PMC
sparser
"LncRNA OTUD6B-AS1 and TRIM16 were poorly expressed (P < 0.01) in CRC tissues and cells and further decreased (P < 0.01) in radioresistant CRC cells."
37184781
sparser
"In this study, we identified that the expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue compared with matched paracancerous tissue, and the downregulation of OTUD6B-AS1 expression was associated with worse clinical characteristics and poor overall survival."
30670025
sparser
"However, to the best of our knowledge, the role of OTUD6B-AS1 in CRC has not yet been determined."
33574932
sparser
"OTUD6B-AS1 inhibits viability, migration, and invasion of thyroid carcinoma by targeting miR-183-5p and miR-21."
32256450
sparser
"Quantitative real-time PCR (qRT-PCR) assays revealed that OTUD6B-AS1 expression was significantly decreased in ccRCC tissue samples and cell lines, suggesting antioncogene functions for OTUD6B-AS1."
30670025
sparser
"These data indicate that mutations in the binding sites of miR-21 and miR-183-5p compromised the binding of miR-21 and miR-183-5p to OTUD6B-AS1."
32256450
sparser
"Moreover, the ACHN and OS-RC-2 cells transfected with the OTUD6B-AS1 overexpression plasmid exhibited higher levels of apoptosis than the corresponding control cells (Fig. xref d and e)."
30670025
sparser
"Interestingly, the elevated T24 cell apoptosis rate induced by As 2 O 3 was further significantly enhanced by lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"Together, these results demonstrate that miR-6734-5p serves as an intermediate in the regulation of IDH2 by lncRNA OTUD6B-AS1."
32952848
sparser
"The binding site between OTUD6B-AS1 and miR-3171 was predicted using Starbase 2.0 ( xref )."
33574932
sparser
"Therefore, OTUD6B-AS1 may serve as a potential novel biomarker and target for the diagnosis and treatment of CRC."
33574932
sparser
"According to our data, OTUD6B-AS1 expression was low in CRC tissues and cells."
33686892
sparser
"These results indicated that OTUD6B-AS1 inhibited the proliferation, invasion and migration of HCT116 cells by targeting miR-3171."
33574932
sparser
"To further interrogate the molecular mechanism by which lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced oxidative damage, we investigated the downstream targets of lncRNA OTUD6B-AS1 by bioinformatic analysis."
32952848
sparser
"The role of OTUD6B-AS1 in thyroid carcinomas has not been reported yet."
32256450
sparser
"Additionally, to demonstrate the mechanism of OTUD6B-AS1 acting as an antioncogene in ccRCC cells, the expression of EMT-related proteins was determined by western blotting."
30670025
sparser
"Furthermore, the protein expression levels of cleaved PARP and Bax exhibited significant increases, while Bcl-2 gene expression exhibited significant decreases in the ACHN and OS-RC-2 transfected with the OTUD6B-AS1 overexpression plasmid (Fig. xref f)."
30670025
sparser
"OTUD6B-AS1."
33593347
sparser
"After constructing the overexpression plasmid of OTUD6B-AS1, transfection efficiency was verified by RT-qPCR, and it was demonstrated that OTUD6B-AS1 expression was significantly upregulated in the Oe-OTUD6B-AS1 group compared with the Oe-NC group ( xref )."
33574932
sparser
"The aim of the present study was to investigate the role of ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) in CRC and its underlying mechanisms."
33574932
sparser
"Furthermore, significant increases in lncRNA OTUD6B-AS1 levels were also observed in xenograft tumor model nude mice treated with As 2 O 3 at 1 mg/kg and 5 mg/kg ( xref )."
32952848
sparser
"However, when we knocked down OTUD6B-AS1 expression in 786-O cells, we observed the opposite result."
30670025
sparser
"Here, we found that lncRNA OTUD6B-AS1 transcription was activated by As 2 O 3 in bladder cancer cells, and these results were validated using a xenograft tumor model, suggesting that the antitumor effects of As 2 O 3 may be via upregulation of lncRNA OTUD6B-AS1; however, the roles of lncRNA OTUD6B-AS1 in exacerbated As 2 O 3 -induced oxidative damage have yet to be elucidated."
32952848
sparser
"Further, functional assays showed that overexpression of lncRNA OTUD6B-AS1 could exacerbate As 2 O 3 -induced cell damage both in vitro and in vivo ."
32952848
sparser
"The results showed that the invasion of ACHN (upper) and OS-RC-2 (lower) cells was notably decreased with OTUD6B-AS1 overexpression (Fig.  xref a and b)."
30670025
sparser
"Additionally, the expression levels of migration-associated proteins were elevated when the HCT116 cells with overexpression of OTUD6B-AS1 were co-transfected with miR-3171 mimic ( xref )."
33574932
sparser
"OTUD6B-AS1 was demonstrated to indicate poor prognosis in ovarian cancer, clear cell renal cell carcinoma (ccRCC), and breast cancer (as an immune-related lncRNA) while its overexpression inhibited ccRCC proliferation ( xref ; xref ; xref )."
34977036
sparser
"In the current work, we evaluated the function of OTUD6B-AS1 in ccRCC progression and found a negative correlation between ccRCC malignancy and OTUD6B-AS1 expression."
30670025
sparser
"We aim to investigate the expression and biological functions of OTUD6B-AS1 in thyroid carcinomas."
32256450
sparser
"OTUD6B-AS1 correlated with tumor size ( p = 0.013, xref ), lymphatic metastasis ( p = 0.015, xref ), and clinical stage ( p = 0.0213 xref )."
32256450
sparser
"OTUD6B-AS1 Is Downregulated in Thyroid Carcinoma."
32256450
sparser
"Then, OTUD6B-AS1 was overexpressed in ACHN and OS-RC-2 cells transfected with the plvx-OTUD6B-AS1."
30670025
sparser
"Here, we investigated the function of lncRNA OTUD6B-AS1 and found that its overexpression clearly suppressed proliferation and elevated apoptosis of bladder cancer cells."
32952848
sparser
"In addition, GSKIP overexpression markedly reversed OTUD6B-AS1 knockdown- or miR-664b-3p overexpression-induced antitumour effects in HCC."
32682012
sparser
"OTUD6B-AS1 was first reported in systemic sclerosis ( xref )."
32256450
sparser
"We found that lncRNA OTUD6B-AS1 expression was significantly higher in the cytoplasm fraction of T24 bladder cancer cells, supporting a role for lncRNA OTUD6B-AS1 function in regulation of miRNA."
32952848
sparser
"In conclusion, OTUD6B-AS1 which acts as a tumor suppressor inhibits the viability, migration, and invasion of thyroid carcinomas by targeting miR-183-5p and miR-21."
32256450
sparser
"In this study, we found that lncRNA TERC, lncRNA CCAT1 and lncRNA OTUD6B-AS1 were upregulated in CRC."
33493136
sparser
"A total of 8 immune-related lncRNAs were obtained, including OTUD6B-AS1(HR = 1.698; 95% CI 1.066–2.707, p  = 0.026), AL122010.1(HR = 0.404; 95% CI 0.209–0.782, p  = 0.007), AC136475.2(HR = 0.596; 95% CI 0.369–0.964, p  = 0.035), AL161646.1(HR = 1.215; 95% CI 0.954–1.549, p  = 0.115), AC245297.3(HR = 0.710;95% CI 0.450–1.121, p  = 0.142), LINC00578(HR = 1.269; 95% CI 1.001–1.609, p  = 0.049), LINC01871(HR = 0.657; 95% CI 0.448–0.964, p  = 0.032), AP000442.2 (HR = 0.335; 95% CI 0.101–1.115, p  = 0.075) (Fig.  xref d, Table xref )."
33225954
sparser
"Further, we show that lncRNA OTUD6B-AS1 induction by As 2 O 3 is dependent on MTF1."
32952848
sparser
"The role of OTUD6B-AS1 in cancer is largely unknown."
32256450
sparser
"Furthermore, the addition of miR-3171 mimics attenuated the effects of OTUD6B-AS1 overexpression on the invasive and migratory abilities of HCT116 cells ( xref )."
33574932
sparser
"Additionally, 12-FRLncRNA signature involved with LINC01871, LINC00393, AC121247.2, LINC02384, LIPE-AS1, HSD11B1-AS1, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 could accurately predict the prognosis of patients with BC, which was confirmed by the training set, validation set, and set from GEO database."
34671639
sparser
"One of the important findings of this study is our identification of two downstream targets of OTUD6B-AS1, miR-183-5p, and miR-21."
32256450
sparser
"Moreover, OTUD6B-AS1 knockdown or miR-664b-3p up-regulation exerted a suppressive effect on Wnt/β-catenin signalling via the down-regulation of GSKIP."
32682012
sparser
"Overexpression of OTUD6B-AS1 inhibited the proliferation, invasion and migration of HCT116 cells."
33574932
sparser
"Risk score = (0.53* OTUD6B-AS1) + (−0.91* AL122010.1) + (−0.52* AC136475.2) + (0.20* AL161646.1) + (−0.34* AC245297.3) + (0.24* LINC00578) + (−0.42* LINC01871) + (−1.10* AP000442.2) (Table  xref )."
33225954
sparser
"To corroborate these findings, we conducted ChIP assays and found that the pull-down with the MTF1 antibody greatly enriched the lncRNA OTUD6B-AS1 promoter region containing putative metal response elements (nucleotides 754 to 854 in the promoter) by almost 5.20-fold relative to control IgG ( P < 0.01), and a reference region (nucleotides 100 to 200 in the promoter) without putative MRE was used as a negative control ( xref ."
32952848
sparser
"More interestingly, another major finding was that the molecular mechanism involved lncRNA OTUD6B-AS1 inhibition of IDH2 expression through stabilization of miR-6734-5p ( xref )."
32952848
sparser
"The correlations between the OTUD6B-AS1 expression level and clinicopathological features were evaluated using the chi-square test."
30670025
sparser
"Additionally, miR-21 and miR-183-5p overexpression had stacked effect to reverse the effect of OTUD6B-AS1 on the viability, migration, and invasion abilities."
32256450
sparser
"To assess whether OTUD6B-AS1 could be used as an indicator for the diagnosis of ccRCC, an ROC curve was built."
30670025
sparser
"Among them, MIR647 and lncRNA OTUD6B-AS1 were found to be significantly correlated with m 6 A regulators METTL3 and YTHDF3, with Pearson’s correlation coefficients of 0.60 ( P < 0.001) and 0.61 ( P < 0.001), respectively."
34950253
sparser
"We investigated the consequences of OTUD6B-AS1 overexpression on the activation of the Wnt/β-catenin pathway in ccRCC cells."
30670025
sparser
"Compared with the control group, the OTUD6B-AS1 overexpression group exhibited decreases in both phosphorylated and nonphosphorylated AKT and glycogen synthase kinase 3β (GSK3β), and the total level of β-catenin was also decreased in both ACHN and OS-RC-2 cells (Fig. xref b)."
30670025
sparser
"We calculated the risk scores for all samples using the following formula: Risk score = (0.309031851 × Exp of TDRKH-AS1) + (0.213481355 × Exp of AC011978.2) + (0.170772984 × Exp of AC110995.1) + (0.051734285 × Exp of OTUD6B-AS1) + (0.046646469 ×Exp of YTHDF3-AS1) + (0.019875191 × Exp of AL512380.1) + (0.019087737 × Exp of MIR4435-2HG) + (–0.037067764 × Exp of HSD11B1-AS1) + (–0.063799579 × Exp of LINC02084) + (–0.162152639 × Exp of TRG-AS1) + (–0.399031951 × Exp of AL451085.3) + (–0.886350422 × Exp of AL109955.1)."
34977036
sparser
"We speculated that OTUD6B-AS1 regulates the migration and invasion of thyroid carcinoma by down-regulating miR-183-5p and miR-21."
32256450
sparser
"Finally, ACHN cells with OTUD6B-AS1 overexpression were subcutaneously injected into nude mice to evaluate the influence of OTUD6B-AS1 on tumor growth in vivo."
30670025
sparser
"miR-3171 is a direct target of OTUD6B-AS1."
33574932
sparser
"A previous study has demonstrated that overexpression of OTUD6B-AS1 could inhibit the proliferation, invasion and migration of renal clear cell carcinoma cells ( xref )."
33574932
sparser
"OTUD6B-AS1 may be an indicator of poor prognosis in patients with ccRCC."
30670025
sparser
"We speculate that higher doses of As 2 O 3 may exceed the tolerance of metallothioneins and that lncRNA OTUD6B-AS1 induces MTF1 in response to uncleared As 2 O 3 , promoting ROS production and oxidative damage."
32952848
sparser
"The effect of lncRNA OTUD6B-AS1 in enhancing As 2 O 3 -induced cytotoxicity was further explored in vivo ."
32952848
sparser
"The correlations between the OTUD6B-AS1 expression levels and clinicopathological features were evaluated using the Mann-Whitney test."
32256450
sparser
"Except for AL161646.1 and LINC00578 ( p  < 0.0001), lncRNAs, including OTUD6B-AS1, AC245297.3, AC136475.2, AL122010.1, AL161646.1 and LINC01871, were high expressed between breast cancers and normal tissues."
33225954
sparser
"Previous study found that OTUD6B-AS1 silenced Wnt/β-catenin pathway in ccRCC ( xref )."
32256450
sparser
"The heatmap revealed that OTUD6B-AS1, AL161646.1, and LINC00578 were highly expressed in the low-risk group, while AC136475.2, AL122010.1, LINC01871, and AP000442.2 were highly expressed in the high-risk group (Fig.  xref g–i)."
33225954
sparser
"Overall, these findings demonstrated that OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells via downregulation of miR-3171, suggesting that OTUD6B-AS1 may serve as a novel biomarker for CRC treatment."
33574932
sparser
"Furthermore, as shown in xref , OTUD6B-AS1 overexpression notably suppressed the invasion and migration of HCT116 cells compared with those of cells in the empty vector (Oe-NC) group."
33574932
sparser
"Furthermore, the impact of OTUD6B-AS1 overexpression on the activation of the Wnt/β-catenin signaling pathway was investigated."
30670025
sparser
"As expected, the results of the RT-qPCR assay ( xref ) indicated that miR-3171 expression was markedly reduced following OTUD6B-AS1 overexpression compared with that in the OE-NC group."
33574932
sparser
"In this study, we selected ACHN and OS-RC-2 cells as they had the lowest OTUD6B-AS1 expression among the ccRCC cell lines (Fig.  xref a)."
30670025
sparser
"Correspondingly, OTUD6B-AS1 overexpression had the opposite effect on HCC cell invasion, colony formation and proliferation."
32682012
sparser
"Using Starbase, it was predicted that OTUD6B-AS1 can bind to miR-3171."
33574932
sparser
"Low miR-206 and high OTUD6B-AS1 expression were associated with significantly poorer overall survival."
34746018
sparser
"As shown in Figures xref and xref , lncRNA OTUD6B-AS1 overexpression markedly increased apoptosis of T24 cells ( P < 0.01)."
32952848
sparser
"The probable relationship between OTUD6B-AS1 and overall survival in ccRCC patients were also determined."
30670025
sparser
"We showed that miR-21 is a direct target of OTUD6B-AS1."
32256450
sparser
"Our study reveals a novel molecular mechanism, mediated by lncRNA OTUD6B-AS1, which may play a key role in regulating the progression of HCC."
32682012
sparser
"Additionally, a luciferase reporter assay was used to verify the potential interaction between OTUD6B-AS1 and microRNA-3171 (miR-3171)."
33574932
sparser
"In systemic sclerosis, OTUD6B-AS1 functions as a regulator of apoptosis ( xref )."
32256450
sparser
"Functionally, overexpression of OTUD6B-AS1 inhibited cell proliferation, migration, invasion and EMT, and promoted cell apoptosis."
33686892
sparser
"TCGA database data show that the expression of the lncRNA OTUD6B-AS1 is downregulated and that OTUD6B-AS1 acts as an antioncogene in a variety of tumors."
30670025
sparser
"In vitro and in vivo evidence indicates that OTUD6B-AS1 functions as a tumor suppressor in ccRCC ( xref )."
32256450
sparser
"Additionally, the long noncoding RNA OTUD6B-AS1 (lncRNA OTUD6B-AS1) of OTUD6B exacerbates oxidative damage in bladder cancer [ xref ] and inhibits the growth, invasion, and migration of thyroid cancer [ xref ]."
34201062
sparser
"As shown in Figures xref and xref , compared with untreated xenograft tumors, more obvious apoptosis was detected in xenograft tumors in which lncRNA OTUD6B-AS1 was overexpressed or those treated with As 2 O 3 ( P < 0.01)."
32952848
sparser
"To further verify this hypothesis, another RT-qPCR assay was performed and revealed that miR-3171 expression was markedly reduced following OTUD6B-AS1 overexpression."
33574932
sparser
"In addition, it has been reported that the overexpression of OTUD6B-AS1 makes hepatocellular carcinoma cells more aggressive through the GSKIP/Wnt/β-catenin signaling pathway ( xref )."
34671639
sparser
"lncRNA ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) is oriented in an antisense direction relative to the protein-coding gene OTUD6B on the opposite DNA strand ( xref )."
33574932
sparser
"Subsequently, we investigated the regulation of miR-6734-5p by lncRNA OTUD6B-AS1."
32952848
sparser
"Numerous studies have shown that ROS underlies the induction of cancer cell apoptosis by As 2 O 3 , and following lncRNA OTUD6B-AS1 overexpression, the elevation of ROS and MDA in T24 cells induced by As 2 O 3 was further significantly enhanced."
32952848
sparser
"Next, we studied whether OTUD6B-AS1 could affect the migration and invasion of ccRCC cells."
30670025
sparser
"OTUD6B-AS1 is a newly identified lncRNA, whose overexpression could suppress tumor growth in kidney cancer, thyroid cancer, and colorectal cancer, but boost tumor growth in hepatocellular carcinoma ( xref )."
36189319
sparser
"First, the subcellular localization of lncRNA OTUD6B-AS1 in T24 cells was determined using a nuclear mass separation assay."
32952848
sparser
"The results of H&E staining indicated that, compared with xenograft tumors with lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment (5 mg/kg), xenograft tumors subjected to both treatments exhibited more severe edema and had increased numbers of vacuolated cells ( xref )."
32952848
sparser
"We also found that the expression of OTUD6B-AS1 was related to the pathologic tumor stage, clinical grade, lymph node status and metastasis status but not the gender and age of patients (Fig. xref c and Table  xref )."
30670025
sparser
"Following OTUD6B-AS1 overexpression, Cell Counting Kit-8 and colony formation assays were used to detect the proliferation ability of HCT116 cells."
33574932
sparser
"Subsequently, lncRNA OTUD6B-AS1 inhibited IDH2 expression by stabilizing miR-6734-5p, exacerbating As 2 O 3 -induced oxidative stress."
32952848
sparser
"In this section, we evaluated the effect of a DNA demethylating agent (5-Aza-CdR) on OTUD6B-AS1 expression at the cellular level."
30670025
sparser
"These data indicate that miR-6734-5p contributes to oxidative stress as a downstream target of lncRNA OTUD6B-AS1."
32952848
sparser
"lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is a tumor-suppressive noncoding RNA in clear cell renal cell carcinoma."
32256450
sparser
"Here, for the first time, we characterize the regulation of As 2 O 3 in the oxidative damage against bladder cancer via lncRNA OTUD6B-AS1."
32952848
sparser
"Therefore, it could be concluded that the overexpression of OTUD6B-AS1 suppressed the proliferation, invasion and migration of CRC cells."
33574932
sparser
"OTUD6B-AS1 was initially reported as active in fibrosis in systemic sclerosis ( xref )."
32256450
sparser
"As for our research, we are the first to report the expression pattern, biological function and potential regulatory mechanism of OTUD6B-AS1 in ccRCC."
30670025
sparser
"Moreover, we screened the potential targets of OTUD6B-AS1 and explored the ceRNAs molecular mechanisms underlying the regulatory effects of OTUD6B-AS1 on thyroid carcinoma cells."
32256450
sparser
"Furthermore, our findings indicated that the lncRNA OTUD6B-AS1 inhibited ccRCC proliferation through the inactivation of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
30670025
sparser
"However, the detailed relevance of OTUD6B-AS1 in hepatocellular carcinoma (HCC) remains undetermined."
32682012
sparser
"LncRNA OTUD6B-AS1 stabilized TRIM16 mRNA via binding to HuR."
37184781
sparser
"Therefore, we sought to determine the interactions between lncRNA OTUD6B-AS1 and miR-6734-5p in evoking oxidative damage against bladder cancer."
32952848
sparser
"For example, OTUD6B-AS1 was down-regulated in the skin tissue of patients with systemic sclerosis [ xref ], which manifests as fibrosis of skin and multiple organs."
34968168
sparser
"Further data confirmed that OTUD6B-AS1 knockdown exerted a tumour-inhibition role in HCC in vivo."
32682012
sparser
"The expression level of OTUD6B-AS1 was measured in 75 paired human ccRCC tissue and corresponding adjacent normal renal tissue samples."
30670025
sparser
"Overexpression of OTUD6B-AS1 significantly decreased the viability, migration, and invasion of thyroid carcinoma cells."
32256450
sparser
"Consistent results in thyroid cancer and ccRCC indicate down-regulation of OTUD6B-AS1 as important for cancer development."
32256450
sparser
"Besides, recent accumulating evidence has indicated that lncRNAs, such as OTUD6B-AS1, URRCC, HOTAIRM1 and MRCCAT1, play important regulatory roles in diverse biological processes in ccRCC xref - xref ."
33854630
sparser
"We also confirmed the effects of OTUD6B-AS1 on ccRCC cells."
30670025
sparser
"Using these HRLs, we finally constructed a novel prognostic model for patients with breast cancer, which consisted of 12 PHRLs: TDRKH-AS1, AC011978.2, AC110995.1, OTUD6B-AS1, YTHDF3-AS1, AL512380.1, MIR4435-2HG, HSD11B1-AS1, LINC02084, TRG-AS1, AL451085.3, and AL109955.1 (RBM38-AS1)."
34977036
sparser
"The novel lncRNA, OTUD6B-AS1, is an important target of gene expression [ xref ]."
32952848
sparser
"However, no significant difference in overall survival status was found between the high OTUD6B-AS1 expression group and the low OTUD6B-AS1 expression group in the KIRP and KICH cohorts (Fig. xref c and d)."
30670025
sparser
"In contrast, in hepatocellular carcinoma (HCC), OTUD6B-AS1 could enhance cell proliferation and invasion ability via the GSKIP/Wnt/β-catenin signaling pathway ( xref )."
34977036
sparser
"To elucidate the role of OTUD6B-AS1 in thyroid carcinoma, SW579 and TPC-1 cells were transfected with OTUD6B-AS1–pcDNA3.1 to overexpress OTUD6B-AS1."
32256450
sparser
"The tumor volumes and weights of xenografts treated with lncRNA OTUD6B-AS1 or As 2 O 3 (5 mg/kg) were clearly reduced and much lower in those treated with both lncRNA OTUD6B-AS1 and As 2 O 3 (all P < 0.01; Figures xref – xref )."
32952848
sparser
"Furthermore, we demonstrated that lncRNA OTUD6B-AS1 was up-regulated in cisplatin-resistant cervical cancer cells, and knocking down OTUD6B-AS1 expression induced re-acquirement of chemosensitivity to cisplatin in cervical cancer cells."
34746018
sparser
"Additionally, overexpression of lncRNA OTUD6B-AS1 significantly reduced tumor size and tumor weight in a xenograft model."
32952848
sparser
"Comprehensive analysis of the lncRNA OTUD6B-AS1 in the TCGA database."
30670025
sparser
"To verify this speculation, we investigated whether the up-regulation of miR-183-5p and miR-21 can compromise the inhibitory effects of OTUD6B-AS1."
32256450
sparser
"The present study demonstrated that OTUD6B-AS1 expression was decreased in CRC cells compared with in a normal intestinal epithelial cell line, which was in accordance with the results of TCGA database analysis."
33574932
sparser
"In this study, we found that the expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue samples and that patients with low OTUD6B-AS1 expression had shorter overall survival than patients with high OTUD6B-AS1 expression, which showed that the different expression level of OTUD6B-AS1 indirectly correlated with survival of patients."
30670025
sparser
"Overexpression of OTUD6B-AS1 inhibits the proliferation, invasion and migration of HCT116 cells."
33574932
sparser
"In addition our data were only obtained with cell lines, and that cell line models have several limitations,so there’s still a lot of work to be done to study OTUD6B-AS1 in depth."
30670025
sparser
"Furthermore, overexpression of OTUD6B-AS1 inhibited the proliferation, migration and invasion of CRC cells."
33574932
sparser
"OTUD6B-AS1 overexpression stabilized TRIM16 via binding to HuR and increased GPX4-mediated ferroptosis, thus attenuating CRC radioresistance."
37184781
sparser
"Similarly, OTUD6B-AS1 played a tumor-suppressive role in thyroid carcinoma ( xref ), bladder carcinoma ( xref ), and CRC ( xref ; xref )."
34977036
sparser
"Collectively, these results offer new insights into the mechanisms involved in As 2 O 3 anticancer activity and demonstrate that lncRNA OTUD6B-AS1 expression can be activated by As 2 O 3 through ROS-mediated upregulation of MTF1."
32952848
sparser
"In conclusion, the current study has elucidated a novel molecular mechanism of As 2 O 3 in evoking oxidative stress in bladder cancer, where MTF1 was increased by As 2 O 3 , thereby activating transcription of lncRNA OTUD6B-AS1."
32952848
sparser
"Subsequently, rescue assays were performed to clarify the regulatory effects of OTUD6B-AS1 and miR-3171 on CRC development."
33574932
sparser
"To test the effect of OTUD6B-AS1 overexpression on the Wnt/β-catenin signaling pathway, we analyzed the expression of several key proteins of this signaling pathway by western blotting."
30670025
sparser
"The rescue assays revealed that miR-3171 mimics markedly reversed the inhibitory effects of OTUD6B-AS1 overexpression on proliferation, invasion and migration of CRC cells."
33574932
sparser
"OTUD6B-AS1 expression is downregulated in HCT116 cells and CRC tissues."
33574932
sparser
"Therefore, OTUD6B-AS1 could be used as an indicator of ccRCC."
30670025
sparser
"Bioinformatic analysis and mechanistical experiments confirmed that OTUD6B-AS1 could act as a competitive endogenous RNA (ceRNA) to upregulate Proline-Rich Nuclear Receptor Coactivator 2 (PNRC2) expression by sequestering miR-21-5p."
33686892
sparser
"miR-183-5p and miR-21 Were Direct Targets of OTUD6B-AS1."
32256450
sparser
"Additionally, an immunofluorescence assay revealed a decrease in the expression levels of proliferation-related protein Ki67 following OTUD6B-AS1 overexpression ( xref )."
33574932
sparser
"Nevertheless, we found that miR-6734-5p mimics did not significantly downregulate lncRNA OTUD6B-AS1 levels ( P > 0.05; xref )."
32952848
sparser
"LncRNA OTUD6B-AS1 is upregulated in clear cell renal cell cancer and enhances tumor proliferation [ xref ]."
33714208
sparser
"In conclusion, to the best of our knowledge, the present study was the first to investigate the role of OTUD6B-AS1 in CRC cells, and to reveal that lncRNA OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells, at least partially, by targeting miR-3171."
33574932
sparser
"We also showed that OTUD6B-AS1 up-regulated the expression of CCND2 by sponging miR-206."
34746018
sparser
"lncRNA OTUD6B-AS1 can inhibit cancer cell proliferation [ xref ]; however, whether lncRNA OTUD6B-AS1 is also involved in oxidative damage triggered by As 2 O 3 in bladder cancer was previously unknown."
32952848
sparser
"Together, these results suggest that lncRNA OTUD6B-AS1 exacerbation of As 2 O 3 -induced cytotoxicity is achieved by enhancement of oxidative stress."
32952848
sparser
"This study explored the mechanism of long non-coding RNA ovarian tumor domain containing 6B-antisense RNA1 (lncRNA OTUD6B-AS1) in CRC radioresistance through tripartite motif 16 (TRIM16)."
37184781
sparser
"Zhuolu Wang et al. found that OTUD6B-AS1 inhibits viability, migration, and invasion of Thyroid Carcinoma by Targeting miR-183-5p and miR-21 [ xref ]."
33225954
sparser
"More notably, through the comprehensive analysis of heatmap, we regarded LINC01871, LINC02384, LIPE-AS1, and HSD11B1-AS1 as protective LncRNAs, while LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were classified as risk LncRNAs."
34671639
sparser
"According to the JASPAR database, the lncRNA OTUD6B-AS1 promoter has a potential binding site for the transcription factor, MTF1 (score, 5.75) ( xref )."
32952848
sparser
"We also evaluated the correlations between the expression levels of OTUD6B-AS1 and the clinicopathological parameters."
32256450
sparser
"Overexpression of OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
30670025
sparser
"Further, RNA-RNA pull-down assays revealed tight binding between lncRNA OTUD6B-AS1 and miR-6734-5p, indicating that lncRNA OTUD6B-AS1 may stabilize miR-6734-5p, preventing its degradation."
32952848
sparser
"Compared with those in normal HIEC cells, the expression levels of OTUD6B-AS1 in CRC cell lines (Caco2, HCT116, LoVo, SW480 and SNU-C1) were markedly downregulated, particularly in HCT116 cells ( xref )."
33574932
sparser
"We found that the expression of OTUD6B-AS1 was up-regulated in HCC tissues, and patients with high levels of OTUD6B-AS1 expression had shorter survival rates than those with low OTUD6B-AS1 expression."
32682012
sparser
"Consequently, levels of miR-6734-5p were elevated in response to increased lncRNA OTUD6B-AS1 levels."
32952848
sparser
"OTUD6B-AS1 overexpression decreased cell survival (P < 0.01), increased γ-H2AX levels (P < 0.01), and elevated ferroptosis and oxidative stress (P < 0.01) after X-ray radiation."
37184781
sparser
"Next, we investigated the molecular process by which As 2 O 3 upregulates lncRNA OTUD6B-AS1 expression."
32952848
sparser
"When analyzing data from TCGA, it was revealed that the levels of OTUD6B-AS1 in CRC tissues were notably decreased compared with those in the adjacent non-tumor tissues, suggesting a potential antitumor effect of OTUD6B-AS1 in CRC progression."
33574932
sparser
"Moreover, overexpression of miR-183-5p and miR-21 compromised the inhibitory effects of OTUD6B-AS1 on viability, migration, and invasion of thyroid carcinoma cells."
32256450
sparser
"The overexpression of lncRNA OTUD6B-AS1 inhibits cell proliferation, migration, and invasion in clear cell renal cell carcinoma ( xref )."
34692706
sparser
"To investigate the impact of OTUD6B-AS1 in vivo, we inoculated empty vector- or plvx-OTUD6B-AS1-transfected ACHN cells into nude mice."
30670025
sparser
"In a series of assays, it was observed that the additional treatment with miR-3171 mimics following OTUD6B-AS1 overexpression in HCT116 cells promoted the proliferation, invasion and migration of HCT116 cells, suggesting that OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells via downregulation of miR-3171 expression."
33574932
sparser
"According to the comprehensive analysis of 12-FRLncRNA signature, LINC01871, LINC02384, LIPE-AS1, and HSD11B1-AS1 were low-risk FRLncRNAs, whereas LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were high-risk FRLncRNAs."
34671639
sparser
"First, we investigated the effect of As 2 O 3 on lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Furthermore, we investigated the effect of OTUD6B-AS1 on cell migration by performing a transwell assay without a Matrigel coating in the upper compartment."
30670025
sparser
"Intriguingly, we established that miR-6734-5p acts as a bridge molecule between lncRNA OTUD6B-AS1 and IDH2."
32952848
sparser
"Additionally, the function and pathological significance of lncRNA OTUD6B-AS1 in NSCLC metastasis were also confirmed in vivo ."
34337014
sparser
"In the kidney clear cell carcinoma (KIRC) cohort, the patients in the high OTUD6B-AS1 expression group had a five-year survival rate 22.8% higher than the five-year survival rate of 15.2% of the patients in the low OTUD6B-AS1 expression group."
30670025
sparser
"Lentivirus-mediated OTUD6B-AS1 overexpression significantly decreased the proliferation of ccRCC cells and promoted the apoptosis of the cells."
30670025
sparser
"Further, upregulation of miR-6734-5p also significantly elevated the T24 cell apoptosis rate in response to lncRNA OTUD6B-AS1 overexpression, relative to lncRNA OTUD6B-AS1 overexpression alone ( P < 0.01) (Figures xref and xref )."
32952848
sparser
"Here, we aimed to evaluate the endogenous cellular mechanisms involved in As 2 O 3 -mediated oxidative damage through regulation of lncRNA ovarian tumor domain containing 6B antisense RNA1 (lncRNA OTUD6B-AS1)."
32952848
sparser
"OTUD6B-AS1 suppresses RCC cell proliferation, induces apoptosis, inhibits cell migration and invasion, and significantly represses tumor growth in mice [ xref ]."
33593347
sparser
"However, one of the yet undetermined mechanisms not addressed in the current study is whether OTUD6B-AS1 can function as a competitive endogenous RNA (ceRNA) to regulate microRNAs (miRNAs) during ccRCC development."
30670025
sparser
"The effects of lncRNA OTUD6B-AS1 were further assessed by pathological examination of harvested tumors."
32952848
sparser
"OTUD6B-AS1 overexpression inhibited the migration and invasion of ccRCC cells in vitro."
30670025
sparser
"Then, qRT-PCR experiments determined an obvious increase in OTUD6B-AS1 expression in the tumor tissue derived from the plvx-OTUD6B-AS1-transfected ACHN cells compared with that derived from the respective control cells (Fig.  xref d)."
30670025
sparser
"The overall survival status significantly differed between the high OTUD6B-AS1 expression group and the low OTUD6B-AS1 expression group (HR = 1.962, 95% CI 1.445–2.664, p  < 0.0001; Fig. xref b)."
30670025
sparser
"In order to verify the predictions of the online programs, luciferase reporter plasmids, psiCHECK-2-WT, containing wild type (WT) OTUD6B-AS1 were created using the psiCHECK-2 vector."
32256450
sparser
"We identified 15 antisense lncRNAs ( RUSC1-AS1 , TBX2-AS1 , PTOV1-AS1 , UBE2D3-AS1 , ERCC8-AS1 , ZMIZ1-AS1 , RNF144A-AS1 , RDH10-AS1 , TRG-AS1 , GSN-AS1 , HMGA2-AS1 , ZNF528-AS1 , OTUD6B-AS1 , COX10-AS1 and SLC16A1-AS1 ) that were upregulated in tumour samples compared to bone sample controls."
34440306
sparser
"Therefore, the present study aimed to investigate whether OTUD6B-AS1 can have an effect on the proliferation, invasion and migration of CRC cells."
33574932
sparser
"As shown in xref , 86.53% of lncRNA OTUD6B-AS1 expression was detected in the cytoplasm fraction in T24 bladder cancer cells, suggesting that the oxidative stress induced by lncRNA OTUD6B-AS1 may be achieved through regulation of miRNA."
32952848
sparser
"To the best of our knowledge, the present study was the first to investigate the role of OTUD6B-AS1 in CRC, and to examine whether OTUD6B-AS1 could affect the proliferation, invasion and migration of CRC cells by regulating miR-3171."
33574932
sparser
"Our work also found that miR-183-5p overexpression promoted the viability, migration, and invasion, reversed OTUD6B-AS1 effect in thyroid carcinomas, which supports the function of miR-183-5p as an oncogene in thyroid carcinomas."
32256450
sparser
"Ma et al. reported OTUD6B-AS1 as an immune-related lncRNA and served as a risk factor in BC patient prognosis ( xref )."
36189319
sparser
"Additionally, miR-21 and miR-183-5p overexpression had stacked effect to reverse the effect of OTUD6B-AS1 on the viability, migration, and invasion abilities ( xref , xref )."
32256450
sparser
"Results: OTUD6B-AS1 was downregulated in thyroid carcinoma tissue samples."
32256450
sparser
"We reported downregulation of lncRNA OTUD6B-AS1 in thyroid cancer."
32256450
sparser
"Therefore, we speculated that lncRNA OTUD6B-AS1 might enhance oxidative stress by downregulating IDH2."
32952848
sparser
"Here, we detected OTUD6B-AS1 expression in CRC tissues and cells by RT-qPCR."
33686892
sparser
"Additional studies are needed to determine whether OTUD6B-AS1 modulates other targets in ccRCC; however, our findings nonetheless provide novel insight into ccRCC pathogenesis as well as a basis for the improvement of individualized treatment for ccRCC patients."
30670025
sparser
"The expression trend of lncRNA OTUD6B-AS1 in CRC and renal cell carcinoma is inconsistent, which may be related to tissue specificity and functional differences."
33493136
sparser
"For instance, lncRNA OTUD6B-AS1 was highly expressed in HCC and associated with HCC patients' prognosis."
34594378
sparser
"No correlation was observed between expression of OTUD6B-AS1 and age ( p = 0.57, xref ), gender ( p = 0.18, xref ), and multifocality ( p = 0.57, xref )."
32256450
sparser
"We revealed a novel mechanism underlying the inhibitory effect of OTUD6B-AS1 on tumor cells."
32256450
sparser
"More importantly, no study has revealed the molecular mechanism and downstream targets of OTUD6B-AS1 until now."
30670025
sparser
"These results further indicate that exacerbation of oxidative stress is key for enhancement of As 2 O 3 -induced cytotoxicity by lncRNA OTUD6B-AS1."
32952848
sparser
"miR-183-5p is another direct target of OTUD6B-AS1."
32256450
sparser
"Similar to the in vitro results, elevated apoptosis rates induced by As 2 O 3 were found to undergo a further significant increase in response to lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"However, Gang Wang et al . [ xref ] found that high OTUD6B-AS1 expression was associated with improved survival and inhibited clear cell renal cell carcinoma proliferation via the Wnt/β-catenin signaling pathway."
33225954
sparser
"To further elucidate whether lncRNA OTUD6B-AS1 could directly bind to miR-6734-5p, RNA pull-down assays were performed and showed that lncRNA OTUD6B-AS1 was more highly enriched in miR-6734-5p pull-down cell lysates, relative to control cells (Figures xref and xref )."
32952848
sparser
"OTUD6B-AS1 locates on chromosome 8q21.3 and has 2179 bp (NR_110439, ENST00000524003.1)."
32256450
sparser
"Similarly, increased OTUD6B-AS1 expression impaired the colony formation capacities of ccRCC cells (Fig. xref f and g)."
30670025
sparser
"Overall, our results prove, for the first time, that the function of lncRNA OTUD6B-AS1 in enhancing As 2 O 3 -mediated oxidative damage is achieved by downregulation of IDH2 through stabilization of its negative regulator, miR-6734-5p."
32952848
sparser
"Cytotoxicity analyses also indicated that both lncRNA OTUD6B-AS1 and miR-6734-5p, or both miR-6734-5p and knockdown of IDH2, clearly increased apoptosis rates relative to lncRNA OTUD6B-AS1, miR-6734-5p, or IDH2 knockdown alone."
32952848
sparser
"It was reported that high OTUD6B-AS1 expression indicates poor prognosis in ovarian cancer [ xref ]."
33225954
sparser
"We found that OTUD6B-AS1 was downregulated in the thyroid carcinoma tissues compared to the para-cancerous tissues ( p < 0.01, xref )."
32256450
sparser
"Collectively, these findings indicate that lncRNA OTUD6B-AS1 is directly regulated by MTF1 at the transcriptional level upon As 2 O 3 treatment."
32952848
sparser
"As 2 O 3 Increases lncRNA OTUD6B-AS1 Expression."
32952848
sparser
"A previous study has demonstrated that OTUD6B-AS1 expression is downregulated in clear cell renal cell carcinoma tissue samples, while overexpression of OTUD6B-AS1 inhibits cell proliferation, migration and invasion of clear cell renal cell carcinoma ( xref )."
33574932
sparser
"Under different physiological and pathological conditions, OTUD6B-AS1 is implicated in different cellular biological functions."
32256450
sparser
"So OTUD6B-AS1, AL161646.1 and LINC00578 were risk factors with HR > 1, while AL122010.1, AC136475.2, AC245297.3, LINC01871 and AP000442.2 were protective factors with an HR < 1."
33225954
sparser
"These results represent new data indicating how lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced oxidative damage via miR-6734-5p-mediated functional inhibition of IDH2."
32952848