IndraLab

Statements

Delta-actitoxin-Avd1a binds OTUD6B. 609 / 609
| 609
sparser
"To the best of our knowledge, the present study was the first to investigate the role of OTUD6B-AS1 in CRC, and to examine whether OTUD6B-AS1 could affect the proliferation, invasion and migration of CRC cells by regulating miR-3171."
33574932
sparser
"As shown in Figures xref and xref , compared with untreated xenograft tumors, more obvious apoptosis was detected in xenograft tumors in which lncRNA OTUD6B-AS1 was overexpressed or those treated with As 2 O 3 ( P < 0.01)."
32952848
sparser
"In conclusion, OTUD6B-AS1 which acts as a tumor suppressor inhibits the viability, migration, and invasion of thyroid carcinomas by targeting miR-183-5p and miR-21."
32256450
sparser
"The lncRNA ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) can promote GPX4-mediated ferroptosis and increase the radiosensitivity of colorectal cancer cells ( xref )."
38846953
sparser
"A previous study revealed that lncRNA OTUD6B-AS1 promoted autophagy in triple-negative breast cancer cells by regulation of the miR-26a/MTDH pathway [ xref ]."
36522638
sparser
"OTUD6B-AS1 is expressed at lower levels in CRC tissues and cells."
39026978
sparser
"Recent accumulating evidence has indicated that lncRNAs, such as OTUD6B-AS1, URRCC, HOTAIRM1 and MRCCAT1, play important regulatory roles in diverse biological processes in ccRCC [ xref – xref ]."
| PMC
sparser
"Mechanistic studies have shown that OTUD6B-AS1 inhibits CRC proliferation by sponging miR-21-5p and upregulating PNRC2 ( xref )."
39026978
sparser
"Cai et al. suggest that the overexpression of OTUD6B-AS1 promotes apoptosis through the OTUD6B-AS1/miR-21-5p/PNRC2 axis ( xref )."
39026978
sparser
"Additionally, the downregulation of lncRNA OTUD6B-AS1, functioning as a competing ceRNA, modulates the expression of miR-26a-5p, inhibits autophagy, and improves paclitaxel-induced cytotoxicity ( xref )."
40223929
sparser
"To determine the involvement of OTUD6B-AS1 in HCC, we compared the expression levels of OTUD6B-AS1 in HCC and normal tissues using the ENCORI Pan-Cancer Analysis Platform."
32682012
sparser
"The dual-luciferase reporter assay demonstrated the binding of miR-3171 and OTUD6B-AS1, since the miR-3171 mimic + OTUD6B-AS1 WT group exhibited lower luciferase activity compared with the mimic-NC + OTUD6B-AS1 WT group ( xref )."
33574932
sparser
"lncRNA ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) is oriented in an antisense direction relative to the protein-coding gene OTUD6B on the opposite DNA strand ( xref )."
33574932
sparser
"NRIR in monocytes of patients with SSc was closely related to IFN response, which further supports the abnormal phenomenon of IFN in SSc. xref Abd-Elmawla et al analyzed the plasma expression profile of lncRNA, and studied its relationship with disease types and related clinical indicators, and proposed plasma SPRY4-IT1, HOTTIP, ANCR and TINCR as new candidate biomarkers for SSc. xref Further, the study by Takata et al unveiled that OTUD6B-AS1 regulated cell proliferation and apoptosis through the expression of cyclinD1 independent of the sense gene, revealing its potential value in regulating the apoptosis of fibroblasts and vascular smooth muscle cells, and may participate in the pathogenesis of SSc. xref Another study by Pachera et al showed that the expression of H19X was dose-dependent with the concentration of TGF-β and could effectively induce fibroblast differentiation and survival, enhance ECM production and tissue progressive fibrosis. xref Of note, the study by Wang et al demonstrated that intrinsic TGF-β activation may result in increased expression of TSIX in the serum of patients with SSc, and that TSIX was able to regulate collagen production. xref Since increased levels of TSIX appear to correlate with levels of skin fibrosis, it has the potential to act as a novel disease biomarker that may also serve as a potential therapeutic target. xref Recently, HOTAIR was found to induce skin fibroblasts differentiation in vitro, driving EZH2-dependent myofibroblast activation in SSc through miRNA 34a-dependent activation of NOTC H. xref Apart from Notch signaling, HOTAIR can also induce fibrosis through Hedgehog signaling. xref The differentially expressed ncRNA00201 and PSMB8-AS1 in blood and MGC12916 in fibroblasts were also confirmed to be important players in the pathogenesis of SSc. xref , xref , xref The involvement of lncRNAs in the pathogenesis of scleroderma is intricate and complicated, which may be through the epigenetic regulation of the gene itself, interacting with miRNAs, or affecting the secretion of important cytokines such as IL-6 and TNFα, xref and participating in regulating the expression of fibrosis-related genes."
35342304
sparser
"A previous study has demonstrated that overexpression of OTUD6B-AS1 could inhibit the proliferation, invasion and migration of renal clear cell carcinoma cells ( xref )."
33574932
sparser
"These data indicate that mutations in the binding sites of miR-21 and miR-183-5p compromised the binding of miR-21 and miR-183-5p to OTUD6B-AS1."
32256450
sparser
"These results suggested that miR-3171 is a direct target of OTUD6B-AS1."
33574932
sparser
"OTUD6B-AS1 suppresses the proliferation, invasion and migration of HCT116 cells by targeting miR-3171."
33574932
sparser
"Moreover, decreased expression of miR-183-5p and miR-21 was observed in SW579 and TPC-1 cells that overexpressed OTUD6B-AS1 ( xref )."
32256450
sparser
"Therefore, it was hypothesized that OTUD6B-AS1 could inhibit CRC progression via inhibition of miR-3171 expression."
33574932
sparser
"To further verify this hypothesis, another RT-qPCR assay was performed and revealed that miR-3171 expression was markedly reduced following OTUD6B-AS1 overexpression."
33574932
sparser
"These results indicated that OTUD6B-AS1 inhibited the proliferation, invasion and migration of HCT116 cells by targeting miR-3171."
33574932
sparser
"As shown in xref , co-transfection of OTUD6B-AS1 overexpression plasmid and miR-3171 mimics reversed the inhibitory effects of OTUD6B-AS1 overexpression alone on the proliferation, colony formation abilities and the Ki67 expression of HCT116 cells."
33574932
sparser
"Furthermore, the addition of miR-3171 mimics attenuated the effects of OTUD6B-AS1 overexpression on the invasive and migratory abilities of HCT116 cells ( xref )."
33574932
sparser
"Additionally, the expression levels of migration-associated proteins were elevated when the HCT116 cells with overexpression of OTUD6B-AS1 were co-transfected with miR-3171 mimic ( xref )."
33574932
sparser
"Taken together, these findings support the prediction that miR-21 and miR-183-5p are the direct target of OTUD6B-AS1."
32256450
sparser
"When analyzing data from TCGA, it was revealed that the levels of OTUD6B-AS1 in CRC tissues were notably decreased compared with those in the adjacent non-tumor tissues, suggesting a potential antitumor effect of OTUD6B-AS1 in CRC progression."
33574932
sparser
"Therefore, the present study aimed to investigate whether OTUD6B-AS1 can have an effect on the proliferation, invasion and migration of CRC cells."
33574932
sparser
"The present study demonstrated that OTUD6B-AS1 expression was decreased in CRC cells compared with in a normal intestinal epithelial cell line, which was in accordance with the results of TCGA database analysis."
33574932
sparser
"Furthermore, overexpression of OTUD6B-AS1 inhibited the proliferation, migration and invasion of CRC cells."
33574932
sparser
"In the present study, a binding site between OTUD6B-AS1 and miR-3171 was predicted using the Starbase database, and miR-3171 expression was upregulated in HCT116 cells."
33574932
sparser
"ShRNA sequences specifically targeting OTUD6B-AS1 or GSKIP were inserted into pLKO."
32682012
sparser
"Additionally, we constructed a OTUD6B-AS1, AL162377.1, AC108449.2, and AF111167.2/hsa-miR-21-5p/KLF9 regulatory axis for KIRC."
35685693
sparser
"The lncRNA OTUD6B-AS1 therefore exerts tumor suppressive effects by inactivating the Wnt/β-catenin pathway (Table  xref )."
33593347
sparser
"Our findings suggest that OTUD6B−AS1 could be a therapeutic target for patients with breast cancer."
37543427
sparser
"Moreover, our findings show that OTUD6B-AS1 promotes tumorigenesis and may serve as an attractive anticancer target for HCC."
32682012
sparser
"Additionally, miR-21 and miR-183-5p overexpression had stacked effect to reverse the effect of OTUD6B-AS1 on the viability, migration, and invasion abilities."
32256450
sparser
"Previous study found that OTUD6B-AS1 silenced Wnt/β-catenin pathway in ccRCC ( xref )."
32256450
sparser
"Our work also found that miR-183-5p overexpression promoted the viability, migration, and invasion, reversed OTUD6B-AS1 effect in thyroid carcinomas, which supports the function of miR-183-5p as an oncogene in thyroid carcinomas."
32256450
sparser
"The result also showed that OTUD6B-AS1 inhibits the viability, migration, and invasion of thyroid carcinomas by targeting oncogenic miR-183-5p."
32256450
sparser
"We revealed a novel mechanism underlying the inhibitory effect of OTUD6B-AS1 on tumor cells."
32256450
sparser
"ARlncRNA OTUD6B-AS1 increases the formation of paclitaxel resistance in triple-negative breast cancer by inhibiting DNA repair (up-regulating genomic instability) and promoting autophagy."
37588204
sparser
"Research has discovered that LncRNA-miR194-2HG ( xref ), LncRNA-DAW ( xref ), LncRNA-NRAV ( xref ), LncRNA-DUXAP10 ( xref ), LncRNA-CRNDE ( xref ), LncRNA OTUD6B-AS1 ( xref ), and miR-550a-5p ( xref ) are overexpression in HCC tissue."
38634048
sparser
"OTUD6B-AS1 and miR-183-5p, constitute another regulatory axis of thyroid carcinomas."
32256450
sparser
"The expression of OTUD6B-AS1, AL162377.1, AC108449.2, AF111167.2, hsa-miR-21-5p, and KLF9 affected the prognosis of KIRC patients."
35685693
sparser
"As shown in the forest map, AC108449.2, AC098484.1, AC092611.2, AC234775.3, OTUD6B-AS1, SPINT1-AS1 and AC007637.1 are considered a protective element, but LINC-PINT, ANKRD10-IT1, ITGB2-AS1, AC021078.1, AC127024.4, AC009120.2, AC090589.3, AC087481.3, LINC01004, AC008105.3, INE1, AC048382.2, AC012170.2, AP002807.1, THUMPD3-AS1, DLEU2, AC069281.2 and AC008870.2 were detrimental parts ( xref )."
37341994
sparser
"OTU Deubiquitinase 6B-Antisense Transcript 1 (OTUD6B-AS1), a novel long non-coding RNA (lncRNA), has recently emerged as a critical regulator in various tumors."
39949926
sparser
"In this work, we compile and discuss findings from a range of studies investigating the expression patterns of OTUD6B-AS1 in different cancers and its consequent effects on tumor behavior, both in vitro and in vivo."
39949926
sparser
"We delve into the mechanisms through which OTUD6B-AS1 influences cancer initiation and progression, focusing on its role in regulating essential cellular processes such as cell growth, migration, invasion, angiogenesis, ferroptosis, and treatment resistance."
39949926
sparser
"Operating through complex interactions with microRNAs (miRNAs), proteins, and pivotal signaling pathways - most notably Wnt/β-catenin - OTUD6B-AS1 exhibits variable roles across cancer types and cellular environments."
39949926
sparser
"OTUD6B-AS1 locates on chromosome 8q21.3 and has 2179 bp (NR_110439, ENST00000524003.1)."
32256450
sparser
"LncRNA OTUD6B-AS1 was downregulated in ccRCC cell lines."
30670025
sparser
"To test the OTUD6B-AS1 expression levels in ccRCC cells, we performed qRT-PCR assays and found that the expression levels of OTUD6B-AS1 were downregulated in the ccRCC cell lines compared with HK-2 cells."
30670025
sparser
"Additionally, the function and pathological significance of lncRNA OTUD6B-AS1 in NSCLC metastasis were also confirmed in vivo ."
34337014
sparser
"Additionally, we assess the clinical relevance of OTUD6B-AS1 expression levels, evaluating its potential as a biomarker for cancer prognosis and diagnosis, as well as a target for therapeutic intervention."
39949926
sparser
"By consolidating existing knowledge, this work aims to highlight the clinical implications of OTUD6B-AS1 and encourage further research in oncology, ultimately contributing to the advancement of targeted cancer therapies."
39949926
sparser
"Cai et al. reported that overexpression of OTUD6B-AS1 inhibits the migration and invasion of CRC cells ( xref )."
39026978
sparser
"2 and StarBase was 8 lncRNAs (AC009299.3, FAM66E, OTUD6B-AS1, NUTM2A-AS1, SNHG1, MIR17HG, BRWD1-IT1 and XIST) ( xref (a))."
36387469
sparser
"Zhang et al. reported that overexpression of OTUD6B-AS1 by binding with HuR stabilizes TRIM16 and increases iron accumulation mediated by GPX4, thus weakening CRC’s radioresistance ( xref )."
39026978
sparser
"Hsa-miR-21-5p, which is closely related to KLF9, was highly expressed in tumors and KIRC patients with high expression of hsa-miR-21-5p have a worse prognosis, suggesting that a negative regulatory relationship between KFL9 and hsa-miR-21-5p and that hsa-miR-21-5p influenced the malignant progression of tumors. ( xref ) Moreover, we further identified four upstream lncRNA targets OTUD6B-AS1, AL162377.1, AC108449.2, and AF111167.2 in StarBase (the inclusion criteria here were the absolute value of correlation coefficient > 0.3 and P < 0.05). ( xref ) They were all closely related to hsa-miR-21-5p. ( xref ) The prognosis of KIRC patients with their high expression levels was better. ( xref ) Therefore, we speculated that there was a negative regulatory relationship between lncRNAs and miRNA."
35685693
sparser
"The OTUD6B-AS1, AL162377.1, AC108449.2, and AF111167.2/hsa-miR-21-5p/KLF9 regulatory axis may play an important role in the progression of KIRC."
35685693
sparser
"OTUD6B-AS1 facilitates paclitaxel resistance by sponging miR-26a-5p to upregulating MTDH, then promoting autophagy and genome instability [ xref ]."
38977630
sparser
"Among the nine key cuproptosis-related lncRNAs we obtained, MME-AS1 has been confirmed to be related to the survival in intrahepatic cholangiocarcinoma [ xref ], while OTUD6B-AS1 is not only associated the progression of various cancers, but also with tumor drugs resistance [ xref – xref ]."
38353835
sparser
"Multivariate Cox regression identified 8 lncRNAs with the best predictive correlation: AL122010.1, OTUD6B-AS1, EGOT, AP005131.2, Z68871.1, AC024361.1, LINC00987, ST7-AS1 ( xref ), and we further visualized the correlation between lncRNAs and mRNAs using Cytoscape ( xref ), showing that Z68871.1, AP005131.2 and AC024361.1 were most closely associated with mRNAs."
39411134
sparser
"We used the RS = (0.542214503 × Z68871.1 expression) + (0.571403384 × OTUD6B-AS1 expression) - (0.8962291962× AC024361.1 expression) - (0.576665716 × LINC0098 expression) - (0.76381661 × AL122010.1 expression) - (0.717527719 × ST7-AS1 expression) - (0.497395506 × AP005131.2 expression) - (0.411082836 × EGO expression) to calculate the score of each patient."
39411134
sparser
"We found that similar to the multifactorial results, high expression levels of AC024361.1, LINC00987, AL122010.1, EGOT, ST7-AS1, and AP005131.2 were correlated with a better prognosis; on the contrary, high expression levels of OTUD6B-AS1 and Z68871.1 were associated with a poor prognosis of patients."
39411134
sparser
"After analysis, AC024361.1, LINC00987, AL122010.1, EGOT, ST7-AS1, and AP005131.2 have been identified as protective factors; OTUD6B-AS1 and Z68871.1 have been identified as risk factors."
39411134
sparser
"Li et al.’s experimentation has shown that overexpression of OTUD6B-AS1 can inhibit the malignant biological characteristics of thyroid cancer cells and is achieved by targeting miR-183-5p ( xref )."
39411134
sparser
"However, there are also studies that overexpression of OTUD6B-AS1 can promote tumor progression, such as lncRNA OTUD6B-AS1 promoting malignant transformation of liver cancer cells through Wnt/β-catenin signaling ( xref )."
39411134
sparser
"For example, OTUD6B-AS1 was down-regulated in the skin tissue of patients with systemic sclerosis [ xref ], which manifests as fibrosis of skin and multiple organs."
34968168
sparser
"Eight of these lncRNAs (OTUD6B-AS1, AL122010.1, AC136475.2, AL161646.1, AC245297.3, LINC00578, LINC01871, AP000442.2) were selected for establishment of the risk prediction model."
33225954
sparser
"A total of 8 immune-related lncRNAs were obtained, including OTUD6B-AS1(HR = 1.698; 95% CI 1.066–2.707, p  = 0.026), AL122010.1(HR = 0.404; 95% CI 0.209–0.782, p  = 0.007), AC136475.2(HR = 0.596; 95% CI 0.369–0.964, p  = 0.035), AL161646.1(HR = 1.215; 95% CI 0.954–1.549, p  = 0.115), AC245297.3(HR = 0.710;95% CI 0.450–1.121, p  = 0.142), LINC00578(HR = 1.269; 95% CI 1.001–1.609, p  = 0.049), LINC01871(HR = 0.657; 95% CI 0.448–0.964, p  = 0.032), AP000442.2 (HR = 0.335; 95% CI 0.101–1.115, p  = 0.075) (Fig.  xref d, Table xref )."
33225954
sparser
"In breast cancer, lncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple-negative breast cancer by regulating autophagy and genomic instability mediated by the miR-26a-5p/MTDH pathway ( xref )."
39744936
sparser
"Previous reports indicated that high expression of hsa-miR-21-5p in six cancers including KIRC was strongly associated with shorter overall survival, which was consistent with our results. xref , xref In addition, hsa-miR-21-5p could drive T helper cells to participate in the kidney function and progression of IgA nephropathy. xref Wang et al xref found that high expression of LncRNA OTUD6B-AS1 represented a favorable prognosis and inhibited the proliferation and progression in KIRC."
35685693
sparser
"Similarly, LncRNA OTUD6B-AS1 might have a role in influencing the progression of KIRC by negatively regulating the high expression of hsa-miR-21-5p, which in turn affects the expression of KLF9."
35685693
sparser
"We identified a OTUD6B-AS1, AL162377.1, AC108449.2, and AF111167.2/hsa-miR-21-5p/KLF9 regulatory axis for KIRC, but further studies were needed to fully elucidate."
35685693
sparser
"Eleven out of 23 lncRNAs including MAPKAPK5-AS1, TRIM52-AS1, CRYZL2P, RNF216P1, CD63-AS1, WAC-AS1, PMS2P1, Alu-mediated p21 transcriptional regulator (APTR), ARRDC1-AS1, PPIAP22, and HEIH were related to HCC, whereas the FCF1P2, OTUD6B-AS1, and MHENCR were related to osteosarcoma, renal cancer, and melanoma, respectively."
38846329
sparser
"Dysregulated Long Non-coding RNAs SNHG15 and OTUD6B-AS1 in Systemic Lupus Erythematosus: Insights from Bioinformatics Analysis and Experimental Validation."
40314733
sparser
"So OTUD6B-AS1, AL161646.1 and LINC00578 were risk factors with HR > 1, while AL122010.1, AC136475.2, AC245297.3, LINC01871 and AP000442.2 were protective factors with an HR < 1."
33225954
sparser
"Except for AL161646.1 and LINC00578 ( p  < 0.0001), lncRNAs, including OTUD6B-AS1, AC245297.3, AC136475.2, AL122010.1, AL161646.1 and LINC01871, were high expressed between breast cancers and normal tissues."
33225954
sparser
"Risk score = (0.53* OTUD6B-AS1) + (−0.91* AL122010.1) + (−0.52* AC136475.2) + (0.20* AL161646.1) + (−0.34* AC245297.3) + (0.24* LINC00578) + (−0.42* LINC01871) + (−1.10* AP000442.2) (Table  xref )."
33225954
sparser
"The heatmap revealed that OTUD6B-AS1, AL161646.1, and LINC00578 were highly expressed in the low-risk group, while AC136475.2, AL122010.1, LINC01871, and AP000442.2 were highly expressed in the high-risk group (Fig.  xref g–i)."
33225954
sparser
"OTUD6B-AS1 and LINC00578 were discovered as prognostic signatures in breast cancer for the first time."
33225954
sparser
"These results further indicate that exacerbation of oxidative stress is key for enhancement of As 2 O 3 -induced cytotoxicity by lncRNA OTUD6B-AS1."
32952848
sparser
"OTUD6B-AS1 is transcribed from the opposite strand of the OTUD6B gene, which is located on chromosome 8 in head-to-head orientation to OTUD6BAS1 [ xref ]."
33225954
sparser
"It was reported that high OTUD6B-AS1 expression indicates poor prognosis in ovarian cancer [ xref ]."
33225954
sparser
"However, Gang Wang et al . [ xref ] found that high OTUD6B-AS1 expression was associated with improved survival and inhibited clear cell renal cell carcinoma proliferation via the Wnt/β-catenin signaling pathway."
33225954
sparser
"Zhuolu Wang et al. found that OTUD6B-AS1 inhibits viability, migration, and invasion of Thyroid Carcinoma by Targeting miR-183-5p and miR-21 [ xref ]."
33225954
sparser
"In the current study, we found that the overall survival was lower under high OTUD6B-AS1 expression."
33225954
sparser
"We speculated that OTUD6B-AS1 affects macrophages and differentiating T cells through miR-21, then affects immune status, and finally affects the occurrence and development of tumor."
33225954
sparser
"Twelve differentially expressed lncRNAs ( AL136531.1, MAPT-AS1, AL606834.2, ST7-AS1, AC024361.1, SEMA3B-AS1, AL136368.1, AC004585.1, LINC01871, AL136295.7, LINC01235 , and OTUD6B-AS1 ) were selected as independent prognostic factors (Table xref )."
33947836
sparser
"The risk score was determined through the application of the multivariate Cox regression formula as follows: risk score = MIR4435-2HG × (0.867022528575273) + EGOT × (-0.235669144798444) + ST7-AS1 × (-1.11551827892176) + PDCD6IP-DT × (− 0.750754400726742) + ERICH6-AS1 × (− 1.20438658732549) + LINC00310 × (0.882320522314723) + OTUD6B-AS1 × (0.423845118028363) + LINC01871 × (− 0.415778456572796)."
39227722
sparser
"The low-risk profile group exhibited elevated expression levels of EGOT, ST7-AS1, PDCD6IP-DT, ERICH6-AS1, and LINC01871, whereas the high-risk group demonstrated increased expression of MIR4435-2HG, LINC00310, and OTUD6B-AS1 (Fig.  xref A)."
39227722
sparser
"For instance, we previously constructed a risk signature based on 6 screened m6A-related lncRNAs, including Z68871.1, AL122010.1, OTUD6B-AS1, AC090948.3, AL138724.1, EGOT ( xref )."
36035182
sparser
"Previously, lncRNA OTUD6B-AS1 was reported to have an antioncogenic role in clear cell renal cell carcinoma, associated with the Wnt/ β -catenin signaling pathway [ xref ]."
32952848
sparser
"The eight Kla-lncRNAs MIR4435-2HG, EGOT, ST7-AS1, PDCD6IP-DT, ERICH6-AS1, LINC00310, OTUD6B-AS1, and LINC01871 were used to categorize BC cases as high- and low-risk groups."
39227722
sparser
"Our results suggest that OTUD6B-AS1 functions as an lncRNA associated with angiogenesis and poses as a risk factor for breast cancer prognosis xref ."
39227722
sparser
"lncRNA has a prominent regulatory role in the Wnt-β-catenin pathway: The increased expression of MSC-AS1 , FAST , JPX , and LALR1 directly or indirectly enhances the stability of β-catenin, thereby activating the Wnt-β-catenin pathway ( xref , xref ); meanwhile, the decreased expression of OTUD6B-AS1 indirectly activates the Wnt-β-catenin pathway ( xref )."
38481861
sparser
"Intriguingly, we established that miR-6734-5p acts as a bridge molecule between lncRNA OTUD6B-AS1 and IDH2."
32952848
sparser
"Tissue lncRNA OTUD6B-AS1 was significantly downregulated in ccRCC patients and exhibited obvious discrimination in the diagnosis (AUC = 0.792, 95% CI: 0.715–0.870) with a sensitivity of 77.3% and specificity of 81.4% [ xref ]."
36732762
sparser
"Among them, MIR647 and lncRNA OTUD6B-AS1 were found to be significantly correlated with m 6 A regulators METTL3 and YTHDF3, with Pearson’s correlation coefficients of 0.60 ( P < 0.001) and 0.61 ( P < 0.001), respectively."
34950253
sparser
"By performing univariate Cox regression analysis on the training set, we identified 13 PRLS (OTUD6B-AS1, AL353622.1, LINC02035, HM13-IT1, FIRRE, NORAD, AC010980.2, AL133243.2, NNT-AS1, PAX8-AS1, POC1B-AS1, U47924.3, and ATP6V0E2-AS1) that had potential prognosis value for UCEC ( xref )."
35228898
sparser
"Li et al. found that miR-26a-5p is a protective element to protect breast cancer from attack, in contrast, OTUD6B-AS1 and MTDH are destructive elements of breast cancer, and miR-26a-5 is down-regulated through OTUD6B-AS1/miR-26a5p/MTDH pathway."
35813295
sparser
"MTDH and OTUD6B-AS1 overexpression promote autophagy and DNA damage, and autophagy defects can increase the short-term sensitivity of tumor cells to chemotherapeutic drugs xref ."
35813295
sparser
"OTUD6B-AS1."
33593347
sparser
"The lncRNA OTUD6B-AS1 (OTUD6B antisense RNA 1) is down-regulated in human RCC tissues, and lower OTUD6B-AS1 expression in RCC tissues correlates with poor patient prognosis [ xref ]."
33593347
sparser
"OTUD6B-AS1 suppresses RCC cell proliferation, induces apoptosis, inhibits cell migration and invasion, and significantly represses tumor growth in mice [ xref ]."
33593347
sparser
"The overall level of PRL expression was higher in cluster2 than in cluster1, especially the expression levels of OTUD6B-AS1 and NORAD ( xref C)."
35228898
sparser
"Mechanistically, OTUD6B-AS1 inactivates the Wnt/β-catenin pathway and suppresses the expression of epithelial-to-mesenchymal transition-related proteins, including E-cadherin, N-cadherin and Snail in RCC cells [ xref ]."
33593347
sparser
"Messemaker et al. have also found that the expression levels of AS lncRNAs, including CTBP1-AS2, AGAP2-AS1, and OTUD6B-AS1, in SSc patients skin tissues showed variations: CTBP1-AS2 and AGAP2-AS1 increased, whereas OTUD6B-AS1 decreased."
35464474
sparser
"A previous study has demonstrated that OTUD6B-AS1 expression is downregulated in clear cell renal cell carcinoma tissue samples, while overexpression of OTUD6B-AS1 inhibits cell proliferation, migration and invasion of clear cell renal cell carcinoma ( xref )."
33574932
sparser
"The overexpression of long non-coding RNA ovarian tumor domain-containing 6B-antisense RNA1 (lncRNA OTUD6B-AS1) stabilizes the tripartite motif 16 (TRIM16) mRNA by binding to the RNA-binding protein human antigen R and increases TRIM16 mRNA levels, promoting GPX4-mediated ferroptosis and inhibiting radioresistance in CRC cells; however, inhibiting ferroptosis attenuates the inhibitory effect of overexpressed lncRNA OTUD6B-AS1 in CRC radioresistance xref ."
39113707
sparser
"Eight ARLs (MAFG−DT, AC097478.1, AL357054.4, AL118556.1, SNHG10, MED14OS, OTUD6B−AS1, and CYTOR) were selected to construct the risk model as a prognostic signature."
37543427
sparser
"Furthermore, qRT-PCR of clinical samples revealed that OTUD6B−AS1 was correlated with prognostic pathological parameters."
37543427
sparser
"OTUD6B−AS1 promoted breast cancer cell proliferation, wound healing, migration, invasion, and human umbilical vein endothelial cells tube formation."
37543427
sparser
"Mechanistically, OTUD6B−AS1 regulated EMT- and angiogenesis-related molecules."
37543427
sparser
"lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is oriented in an antisense direction relative to the protein-coding gene OTUD6B on the opposite DNA strand."
32256450
sparser
"Kaplan-Meier analysis was used to evaluate the relationship between OTUD6B-AS1 expression in ccRCC and patient survival, and the results showed that lower OTUD6B-AS1 expression was associated with poor survival."
30670025
sparser
"Exploration was conducted on the expression of OTUD6B–AS1 in breast cancer tissues, as well as its impact on cellular behaviors and regulation of signaling pathways associated with EMT and angiogenesis."
37543427
sparser
"The low-risk group revealed higher expression of MAFG–DT, AC097478.1, AL357054.4, AL118556.1, SNHG10, and MED14OS, but lower expression of OTUD6B–AS1 and CYTOR ( xref )."
37543427
sparser
"Association of OTUD6B−AS1 with invasive pathological parameters."
37543427
sparser
"Among the eight prognosis-related lncRNAs, OTUD6B−AS1 was selected for further qRT–PCR validation because it showed the largest HR in the univariate Cox regression analysis and the largest coefficient in the formula of multivariate analysis."
37543427
sparser
"The expression level of OTUD6B−AS1 was even higher in breast cancer tissues with axillary lymph node metastasis (ALNM) than those without ALNM ( xref and xref )."
37543427
sparser
"Moreover, higher expression of OTUD6B−AS1 was observed in breast cancer tissues with larger tumor size (T stage) ( xref ), and high expression of OTUD6B−AS1 was positively related with advanced tumor stage ( xref and xref )."
37543427
sparser
"OTUD6B−AS1 was upregulated to a greater extent in breast cancer tissues with high (≥ 30%) Ki-67 expression compared with those with low (< 30%) Ki-67 expression [ xref , xref ] ( xref )."
37543427
sparser
"Interestingly, OTUD6B−AS1 expression was also correlated with molecular subtypes ( xref )."
37543427
sparser
"These results suggested that OTUD6B−AS1 is correlated with aggressive pathological parameters, which are associated with a poor prognosis."
37543427
sparser
"In addition, lncRNA OTUD6B-AS1 (ovarian tumor domain containing 6B antisense RNA1) level was found to decline in CRC, and OTUD6B-AS1 suppresses cell proliferation, EMT and metastasis, and strengthens [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33901505
sparser
"The survival time of the patients with high OTUD6B-AS1 expression ( n  = 26) was longer than that of the patients with low OTUD6B-AS1 expression ( n  = 26) ( P  < 0.0001, Fig. xref e)."
30670025
sparser
"OTUD6B−AS1 promoted breast cancer progression and involved in EMT- and angiogenesis-related signaling."
37543427
sparser
"To investigate the function of OTUD6B−AS1, the BT474 breast cancer cell line was transfected with OTUD6B−AS1 overexpression or empty plasmids."
37543427
sparser
"Overexpression of OTUD6B−AS1 endowed breast cancer cells with increased proliferation ( xref ), wound healing ( xref ), and migratory and invasion ( xref , xref ) abilities."
37543427
sparser
"Subsequently, the tube formation assay was conducted. xref demonstrated that OTUD6B−AS1 enhanced the tube formation capacity of HUVEC."
37543427
sparser
"Risk Score = MCF2L-AS1 × (−0.6581) + USP30-AS1 × (−0.9132) + OTUD6B-AS1 × (1.4151) + MAPT-AS1 × (−0.8676) + PRR34-AS1 × (−1.0859) + DLGAP1-AS1 × (−1.5074)."
37110517
sparser
"The expression of E-cadherin was reduced by OTUD6B−AS1, while the expressions of HIF-1α, MMP1, SMAD5, Snail, Twist1, and VEGFA were increased ( xref )."
37543427
sparser
"This indicates that OTUD6B−AS1 plays a role in controlling EMT and angiogenesis."
37543427
sparser
"In NHBE cells, lncRNAs NUTM2B-AS1 (NUTM2B antisense RNA 1) (log2FC = 6.15, FDR value = 3.38E-02) and OTUD6B-AS1 (OTUD6B antisense RNA 1) were both found to be upregulated (log2FC = 5.66, FDR value = 1.53E-03)."
36877189
sparser
"In line with our discoveries, the lncRNA OTUD6B−AS1 was found to enhance resistance to paclitaxel in breast cancer [ xref ], as well as being associated with an unfavorable prognosis [ xref ]."
37543427
sparser
"Among the eight prognosis-related lncRNAs in this study, OTUD6B−AS1 was selected for further research because it showed the largest HR value in the univariate and multivariate analyses."
37543427
sparser
"The cDNA sequences of OTUD6B-AS1 or GSKIP mRNA 3′-UTR harbouring wild-type or mutant miR-664b-3p binding sites were inserted into pmirGLO plasmids (Promega, Madison, WI, USA)."
32682012
sparser
"For the detection of Wnt signalling, HCC cells were co-transfected with a TCF/LEF1 luciferase reporter plasmid, a plasmid encoding Renilla luciferase and OTUD6B-AS1 shRNA-containing plasmids or OTUD6B[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Our study found that lncRNA OTUD6B-AS1 was upregulated whereas CANX gene was downregulated ( xref )."
36877189
sparser
"Thus, overexpression of lncRNA OTUD6B-AS1 in NHBE cells may provide protective functions for maintaining cellular homeostasis."
36877189
sparser
"Our results proved that higher expression of OTUD6B−AS1 was positively related with larger tumor size, positive lymph node metastasis, more advanced tumor stage, and higher Ki-67 expression in breast cancer, indicating poor survival of patients with breast cancer."
37543427
sparser
"OTUD6B−AS1 also promoted breast cancer cell proliferation, wound healing, migration, invasion, and HUVEC tube formation, and mechanistically regulated EMT- and angiogenesis-related molecules."
37543427
sparser
"OTUD6B−AS1 potentially facilitated the advancement of breast cancer by regulating molecules associated with EMT and angiogenesis, indicating a potential target for therapeutic intervention in breast cancer."
37543427
sparser
"The lncRNA ovarian tumour domain containing 6B antisense RNA1 (OTUD6B-AS1) has been identified as a new tumour-associated lncRNA present in multiple tumour types [ 20 , 21 ]."
32682012
sparser
"The low-risk group highly expressed MCF2L-AS1, MAPT-AS1, USP30-AS1, PRR34-AS1, and DLGAP1-AS1, whereas the high-risk group highly expressed OTUD6B-AS1, as indicated in the heat map ( xref A)."
37110517
sparser
"However, the involvement of OTUD6B-AS1 in HCC is unknown."
32682012
sparser
"This study was performed to determine the expression, function and potential regulatory mechanism of OTUD6B-AS1 in HCC."
32682012
sparser
"The cDNA sequences encoding full length OTUD6B-AS1 were sub-cloned into pLVX plasmids to obtain OTUD6B-AS1 expression plasmids."
32682012
sparser
"Notably, high levels of expression of OTUD6B-AS1 were also detected in multiple HCC cell lines relative to normal hepatocytes HL-7702 ( Fig. 1 D)."
32682012
sparser
"Mechanistically, OTUD6B-AS1 acts as a ceRNA to sponge and sequester miR-21-5p in CRC, leading to proline-rich nuclear receptor coactivator 2 (PNRC2) overexpression, which certifies the inhibitory func[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33901505
sparser
"We found that OTUD6B-AS1 expression was significantly higher in HCC tissues than that in normal tissues ( Fig. 1 A)."
32682012
sparser
"Notably, Kaplan–Meier analysis revealed that patients with high levels of expression of OTUD6B-AS1 had a decreased rate of survival relative to those with low levels of expression of OTUD6B-AS1 ( Fig.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Moreover, we examined the expression of OTUD6B-AS1 in clinical samples of HCC by qRT-PCR."
32682012
sparser
"Two miRNAs, miR-183-5p and miR-21, were found to be potential targets of OTUD6B-AS1 ( xref )."
32256450
sparser
"Our data showed that OTUD6B-AS1 expression was markedly up-regulated in tumour tissues of HCC compared with normal non-tumorous tissues ( Fig. 1 C)."
32682012
sparser
"The sequences of the binding sites of lncRNA OTUD6B-AS1 with miR-21 and miR-183-5p were shown in xref ."
32256450
sparser
"Additional research has uncovered a link between elevated expression levels of lncRNAs like DCST1-AS1 [ xref ], MAPT-AS1 [ xref ], LINK00160 [ xref ], lncRNA OTUD6B-AS1 [ xref ] and the development of PTX resistance in BC via the regulation of specific genes and pathways."
38463803
sparser
"Collectively, these data indicate that OTUD6B-AS1 expression is elevated in HCC."
32682012
sparser
"Furthermore, significant increases in lncRNA OTUD6B-AS1 levels were also observed in xenograft tumor model nude mice treated with As 2 O 3 at 1 mg/kg and 5 mg/kg ( xref )."
32952848
sparser
"Further, lncRNA OTUD6B-AS1 inhibited mitochondrial NADP + -dependent isocitrate dehydrogenase 2 (IDH2) expression by stabilizing miR-6734-5p, which contributed to cytotoxicity by enhancing oxidative stress."
32952848
sparser
"To assess whether OTUD6B-AS1 is involved in regulating HCC cell proliferation and invasion, we performed loss-of-function experiments in which OTUD6B-AS1 was knocked-down using the gene silencing meth[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"The expression of OTUD6B-AS1 in Hep3B and SNU-475 cells was markedly decreased by transfecting plasmids expressing OTUD6B-AS1 shRNAs ( Fig. 2 A)."
32682012
sparser
"We then tested the effect of OTUD6B-AS1 on the proliferation of Hep3B and SNU-475 cells using a CCK-8 assay."
32682012
sparser
"In BC patients, only the high expression of OTUD6B-AS1 was linked with poor prognosis, whereas in BC patients, the high expression of the other five lncRNAs was linked with a good prognosis ( xref K–P)."
37110517
sparser
"Data revealed that silencing OTUD6B-AS1 markedly decreased the proliferative capacity of Hep3B and SNU-475 cells ( Fig. 2 B)."
32682012
sparser
"A colony formation assay confirmed that OTUD6B-AS1 silencing had a suppressive effect on cell proliferation, since fewer colonies were formed when OTUD6B-AS1 was silenced in Hep3B and SNU-475 cells ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Moreover, the silencing of OTUD6B-AS1 caused a significant decrease in the capacity of Hep3B and SNU-475 cells to invade ( Fig. 2 E and F)."
32682012
sparser
"Correspondingly, the up-regulation of OTUD6B-AS1 remarkably accelerated the proliferation and invasion of Hep3B and SNU-475 cells ( Fig. 3 A–F)."
32682012
sparser
"In short, OTUD6B-AS1 is involved in HCC by mediating cell proliferation and invasion."
32682012
sparser
"To determine the molecular mechanism by which OTUD6B-AS1 regulates the progression of HCC, we performed a bioinformatics analysis to predict the downstream miRNA targets of OTUD6B-AS1."
32682012
sparser
"Intriguingly, we found that OTUD6B-AS1 had a putative binding site for miR-664b-3p ( Fig. 4 A)."
32682012
sparser
"To confirm whether miR-664b-3p directly binds to OTUD6B-AS1, a luciferase reporter assay was carried out."
32682012
sparser
"The data demonstrated that the up-regulation of miR-664b-3p caused a significant decrease in the luciferase activity of cells transfected with the wild-type OTUD6B-AS1 reporter plasmid ( Fig. 4 B)."
32682012
sparser
"However, miR-664b-3p overexpression had no significant impact on in luciferase activity of cells transfected with mutant OTUD6B-AS1 ( Fig. 4 B)."
32682012
sparser
"To verify the interaction between miR-664b-3p and OTUD6B-AS1, we performed an RNA pull-down assay using biotinylated miR-664b-3p mimics."
32682012
sparser
"A prognostic model of aging-related lncRNAs including six lncRNA molecules (MCF2L-AS1, USP30-AS1, OTUD6B-AS1, MAPT-AS1, PRR34-AS1 and DLGAP1-AS1) was constructed."
37110517
sparser
"Results demonstrated that biotinylated miR-664b-3p mimics precipitated OTUD6B-AS1 in HCC cells ( Fig. 4 C)."
32682012
sparser
"Moreover, the knockdown of OTUD6B-AS1 markedly up-regulated the expression of miR-664b-3p ( Fig. 4 D), while OTUD6B-AS1 overexpression significantly decreased the expression of miR-664b-3p ( Fig. 4 E)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Li PP et al. reported that overexpression of the lncRNA OTUD6B-AS1 increased paclitaxel resistance in triple-negative breast cancer patients through the downregulation of miR-26a-5p and the overexpression of metadherin (MTDH)."
39253383
sparser
"Overall, these results suggest that OTUD6B-AS1 acts as a molecular sponge of miR-664b-3p."
32682012
sparser
"Similarly, the knockdown of OTUD6B-AS1 up-regulation also down-regulated levels of active β-catenin ( Fig. 6 D and E) and blocked the activation of Wnt/β-catenin signalling in HCC cells ( Fig. 6 F)."
32682012
sparser
"On the contrary, the down-regulation of miR-664–3p or overexpression of OTUD6B-AS1 markedly enhanced the activation of Wnt/β-catenin signalling in HCC cells ( Supplementary Fig. 1 )."
32682012
sparser
"To confirm whether the miR-664b-3p/GSKIP axis modulates OTUD6B-AS1-mediated effects, we assessed the effect of inhibiting miR-664b-3p on OTUD6B-AS1 knockdown-induced inhibition of HCC cell proliferati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Results revealed that the inhibition of miR-664b-3p markedly reversed the suppressive effect of OTUD6B-AS1 knockdown on Wnt/β-catenin signalling, cell proliferation and invasion of HCC cells ( Fig. 7 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Moreover, we found that OTUD6B-AS1 knockdown decreased the expression of GSKIP ( Fig. 7 E), and the restoration of GSKIP also partially abolished the suppressive effect of OTUD6B-AS1 knockdown on HCC [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"In addition, miR-664b-3p overexpression or GSKIP knockdown markedly reversed the OTUD6B-AS1 overexpression-induced promotion effect on HCC cell proliferation and invasion ( Supplementary Fig. 2 )."
32682012
sparser
"Collectively, these data imply that OTUD6B-AS1 regulates HCC cell proliferation and invasion through modulation of the miR-664b-3p/GSKIP axis."
32682012
sparser
"Moreover, the knockdown of OTUD6B-AS1 up-regulated the expression of miR-664b-3p ( Fig. 8 C and D), while it down-regulated the expression of GSKIP and active β-catenin in tumour tissues ( Fig. 8 E)."
32682012
sparser
"The present study has, for the first time, uncovered a key function of the lncRNA, OTUD6B-AS1, in HCC."
32682012
sparser
"Our data showed that high levels of expression of OTUD6B-AS1 in HCC tissues predicted low survival rates for HCC patients."
32682012
sparser
"Functional assays revealed that the silencing of OTUD6B-AS1 repressed proliferation, colony formation and the invasion of HCC cells, whereas its overexpression produced the opposite effect."
32682012
sparser
"Moreover, we determined that OTUD6B-AS1 acts as a molecular sponge of miR-664b-3p."
32682012
sparser
"Therefore, by sequestering miR-664b-3p, OTUD6B-AS1 accelerated the activation of Wnt/β-catenin signalling via the up-regulation of GSKIP."
32682012
sparser
"Multiple tumor types include the lncRNA ovarian tumor domain that contains 6B antisense RNA1 (OTUD6B-AS1)."
37110517
sparser
"Survival rates are lower in hepatocellular carcinoma patients with high OTUD6B‐AS1 expression xref ."
37781080
sparser
"Sixteen lncRNAs (ACTA2-AS1, CARD8-AS1, HCP5, HHIP-AS1, HOTAIRM1, ITGB2-AS1, LINC00324, LINC00605, LINC01503, LINC01547, MIR31HG, MIR155HG, OTUD6B-AS1, PSPXD2A1, SH3PMG3-AS1, SH3, and ZBED5-AS1) were found to be significantly correlated with the OS rate of patients with ovarian cancer (OC) [ xref ]."
34258248
sparser
"Currently, the role of OTUD6B-AS1 in disease progression has been highlighted."
32682012
sparser
"RNA sequence analysis has shown that OTUD6B-AS1 expression levels are decreased in the skin tissues of systemic individuals with sclerosis and that OTUD6B-AS1 participates in the modulation of prolife[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"OTUD6B-AS1 has also been discovered to be a new tumor-associated lncRNA [ xref ]."
37110517
sparser
"Interestingly, recent studies have revealed an important role of OTUD6B-AS1 in cancer [ 20 ]."
32682012
sparser
"Besides, Kong et al. [ xref ] confirmed in their research that silencing OTUD6B-AS1 obviously reduced the proliferation and invasion of HCC cells."
33604387
sparser
"It has been reported that OTUD6B-AS1 expression is dysregulated in a wide range of tumour tissues [ 20 ]."
32682012
sparser
"Several reports are consistent with our view that OTUD6B-AS1 can serve as a predictor of BC prognosis [ xref , xref ]."
37110517
sparser
"Particularly, low levels of OTUD6B-AS1 expression have frequently been detected in clear cell renal cell carcinoma and patients with the low expression of OTUD6B-AS1 had decreased survival rates compa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Moreover, the up-regulation of OTUD6B-AS1 repressed the proliferation and invasion of clear cell renal cell carcinoma cells in vitro , and impeded tumour growth in vivo [ 20 ]."
32682012
sparser
"Therefore, these findings suggest that OTUD6B-AS1 acts a tumour-suppressive lncRNA."
32682012
sparser
"However, recent reports have shown that high levels of expression of OTUD6B-AS1 predicts low survival rates in ovarian cancer patients [ 21 ], indicating a tumour-promotion role of OTUD6B-AS1."
32682012
sparser
"In accordance with this study, our data demonstrated that OTUD6B-AS1 was highly expressed in HCC, and also predicted a low survival rate in HCC patients."
32682012
sparser
"The overexpression of lncRNA OTUD6B-AS1 inhibits cell proliferation, migration, and invasion in clear cell renal cell carcinoma ( xref )."
34692706
sparser
"Moreover, our results revealed that the down-regulation of OTUD6B-AS1 caused a significant decrease in HCC cell proliferation and invasion."
32682012
sparser
"On the other hand, the up-regulation of OTUD6B-AS1 accelerated proliferation and invasion in HCC cells."
32682012
sparser
"Notably, we also found that the knockdown of OTUD6B-AS1 repressed the tumour growth of HCC cells in vivo ."
32682012
sparser
"Their analysis of the TCGA cohort led to the identification of a six aging-related lncRNA signature, comprising MCF2L-AS1, USP30-AS1, OTUD6B-AS1, MAPT-AS1, PRR34-AS1, and DLGAP1-AS1."
38067461
sparser
"In conclusion, these data demonstrate that OTUD6B-AS1 sequesters miR-664b-3p, and the expression of OTUD6B-AS1 accelerates HCC cell proliferation and invasion, which is associated with the up-regulati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Therefore, our study confirms a tumour-promotion function of OTUD6B-AS1 in HCC."
32682012
sparser
"The contrasting effect of OTUD6B-AS1 expression in tumorigenesis indicates that the expression status and precise function of OTUD6B-AS1 may be strictly dependent on the tumour type involved."
32682012
sparser
"Recently, the involvement of dysregulated long noncoding RNA ovarian tumor domain containing 6B antisense RNA1 (lncRNA OTUD6B-AS1) in tumorigenesis is established."
32952848
sparser
"Thus, the precise role of OTUD6B-AS1 requires further study in different tumour types."
32682012
sparser
"Here, for the first time, we characterize the regulation of As 2 O 3 in the oxidative damage against bladder cancer via lncRNA OTUD6B-AS1."
32952848
sparser
"Interestingly, we identified miR-664b-3p as a target of OTUD6B-AS1, which we verified using both a luciferase reporter assay and an RNA pull-down assay."
32682012
sparser
"Our data showed that the overexpression of OTUD6B-AS1 decreased the expression of miR-664b-3p, while the knockdown of OTUD6B-AS1 increased the expression of miR-664b-3p."
32682012
sparser
"As 2 O 3 could activate lncRNA OTUD6B-AS1 transcription in bladder cancer cells, and these findings were validated in a xenograft tumor model."
32952848
sparser
"Moreover, the inhibition of miR-664b-3p significantly reversed OTUD6B-AS1 knockdown-mediated tumour-inhibition effect in HCC, indicating that miR-664b-3p contributes to the regulation of OTUD6B-AS1-me[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Therefore, the high levels of OTUD6B-AS1 expression may contribute to decreased miR-664b-3p-mediated inhibition of tumorigenesis, indicating that the OTUD6B-AS1/miR-664b-3p axis is a key regulator of [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32682012
sparser
"Functional assays showed that lncRNA OTUD6B-AS1 dramatically exacerbated As 2 O 3 -mediated oxidative damage by inducing oxidative stress."
32952848
sparser
"Mechanistically, As 2 O 3 increased levels of metal-regulatory transcription factor 1 (MTF1), which regulates lncRNA OTUD6B-AS1, in response to oxidative stress."
32952848
sparser
"Our study suggests that OTUD6B-AS1 has the potential to be utilised as a prognostic biomarker for HCC."
32682012
sparser
"These data suggest that As 2 O 3 activates lncRNA OTUD6B-AS1 transcription in bladder cancer."
32952848
sparser
"lncRNA OTUD6B-AS1 Overexpression Inhibits Bladder Cancer Progression."
32952848
sparser
"Subsequently, we overexpressed lncRNA OTUD6B-AS1 8.79-fold by transfecting T24 cells with a lentiviral vector ( xref ), to quantitatively evaluate the biological function of lncRNA OTUD6B-AS1 in As 2 O 3 -induced cytotoxicity in vitro ."
32952848
sparser
"The results of the CCK-8 assay ( xref ) showed that both lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment (20  μ mol/L) significantly decreased T24 cell viability to 40% ( P < 0.01)."
32952848
sparser
"As shown in Figures xref and xref , lncRNA OTUD6B-AS1 overexpression markedly increased apoptosis of T24 cells ( P < 0.01)."
32952848
sparser
"Interestingly, the elevated T24 cell apoptosis rate induced by As 2 O 3 was further significantly enhanced by lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"Western blotting showed that, under lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment (20  μ mol/L), the expression of Bax and Caspase3 was significantly increased, while levels of Bcl-2 were significantly decreased relative to lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment ( P < 0.01; Figures xref – xref )."
32952848
sparser
"The effect of lncRNA OTUD6B-AS1 in enhancing As 2 O 3 -induced cytotoxicity was further explored in vivo ."
32952848
sparser
"The tumor volumes and weights of xenografts treated with lncRNA OTUD6B-AS1 or As 2 O 3 (5 mg/kg) were clearly reduced and much lower in those treated with both lncRNA OTUD6B-AS1 and As 2 O 3 (all P < 0.01; Figures xref – xref )."
32952848
sparser
"In addition, levels of lncRNA OTUD6B-AS1 in xenograft tumors were confirmed to increase with lncRNA OTUD6B-AS1 overexpression, As 2 O 3 treatment, or both (all P < 0.01; xref )."
32952848
sparser
"The effects of lncRNA OTUD6B-AS1 were further assessed by pathological examination of harvested tumors."
32952848
sparser
"The results of H&E staining indicated that, compared with xenograft tumors with lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment (5 mg/kg), xenograft tumors subjected to both treatments exhibited more severe edema and had increased numbers of vacuolated cells ( xref )."
32952848
sparser
"Together, these results further indicate that lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced cytotoxicity."
32952848
sparser
"Similar to the in vitro results, elevated apoptosis rates induced by As 2 O 3 were found to undergo a further significant increase in response to lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"Xenograft tumors with both lncRNA OTUD6B-AS1 overexpression and As 2 O 3 treatment showed significantly weaker Ki-67 expression ( xref ) and significantly stronger p57 expression ( xref ), compared with xenograft tumors with lncRNA OTUD6B-AS1 overexpression or As 2 O 3 treatment alone."
32952848
sparser
"As 2 O 3 Upregulates lncRNA OTUD6B-AS1 Expression via MTF1."
32952848
sparser
"To gain insights into the molecular mechanism underlying lncRNA OTUD6B-AS1 exacerbation of As 2 O 3 -induced cytotoxicity, levels of oxidative stress were evaluated in T24 cells."
32952848
sparser
"Interestingly, following lncRNA OTUD6B-AS1 overexpression, levels of ROS and MDA in T24 cells treated with As 2 O 3 were significantly enhanced ( P < 0.01); however, the activities of the antioxidant enzymes SOD and GSH-Px showed the opposite trend, suggesting an impaired antioxidant defense system (Figures xref and xref )."
32952848
sparser
"In addition, we explored the expression of Nrf2 and found that Nrf2 levels in cytoplasm were clearly elevated following lncRNA OTUD6B-AS1 overexpression or treatment with As 2 O 3 ; however, Nrf2 in the nucleus showed the opposite trend (Figures xref – xref )."
32952848
sparser
"Furthermore, ROS and MDA production also increased significantly in response to lncRNA OTUD6B-AS1 overexpression ( P < 0.01)."
32952848
sparser
"According to the JASPAR database, the lncRNA OTUD6B-AS1 promoter has a potential binding site for the transcription factor, MTF1 (score, 5.75) ( xref )."
32952848
sparser
"To corroborate these findings, we conducted ChIP assays and found that the pull-down with the MTF1 antibody greatly enriched the lncRNA OTUD6B-AS1 promoter region containing putative metal response elements (nucleotides 754 to 854 in the promoter) by almost 5.20-fold relative to control IgG ( P < 0.01), and a reference region (nucleotides 100 to 200 in the promoter) without putative MRE was used as a negative control ( xref ."
32952848
sparser
"In addition, we assessed whether MTF1 could activate transcription from the lncRNA OTUD6B-AS1 promoter using a dual-luciferase reporter assay."
32952848
sparser
"As shown in xref , the luciferase activity driven by the WT promoter sequence was markedly reduced by MTF1 knockdown, indicating that the WT sites are required for binding of MTF1 to the lncRNA OTUD6B-AS1 promoter."
32952848
sparser
"Together, these results suggest that lncRNA OTUD6B-AS1 exacerbation of As 2 O 3 -induced cytotoxicity is achieved by enhancement of oxidative stress."
32952848
sparser
"Next, we investigated the molecular process by which As 2 O 3 upregulates lncRNA OTUD6B-AS1 expression."
32952848
sparser
"To further verify whether MTF1 is responsible for As 2 O 3 regulation of lncRNA OTUD6B-AS1 transcription, endogenous MTF1 was knocked down using shRNA ( xref )."
32952848
sparser
"Significantly reduced lncRNA OTUD6B-AS1 levels were observed in MTF1 knockdown cells ( P < 0.01), and the elevated lncRNA OTUD6B-AS1 levels in As 2 O 3 -treated T24 cells were significantly attenuated by MTF1 knockdown ( P < 0.01) ( xref )."
32952848
sparser
"These data suggest that lncRNA OTUD6B-AS1 transcription is regulated by MTF1."
32952848
sparser
"OTUD6B-AS1 lncRNA induces oxidative stress to increase cancer cell damage, specifically by increasing metal-regulated transcription factor 1 (MTF1), which inhibits the expression of mitochondrial NADP + -dependent IDH2 [ xref ]."
36497259
sparser
"Collectively, these findings indicate that lncRNA OTUD6B-AS1 is directly regulated by MTF1 at the transcriptional level upon As 2 O 3 treatment."
32952848
sparser
"lncRNA OTUD6B-AS1 Downregulates IDH2 Expression by Stabilizing miR-6734-5p."
32952848
sparser
"To further interrogate the molecular mechanism by which lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced oxidative damage, we investigated the downstream targets of lncRNA OTUD6B-AS1 by bioinformatic analysis."
32952848
sparser
"First, the subcellular localization of lncRNA OTUD6B-AS1 in T24 cells was determined using a nuclear mass separation assay."
32952848
sparser
"To further elucidate whether lncRNA OTUD6B-AS1 could directly bind to miR-6734-5p, RNA pull-down assays were performed and showed that lncRNA OTUD6B-AS1 was more highly enriched in miR-6734-5p pull-down cell lysates, relative to control cells (Figures xref and xref )."
32952848
sparser
"As shown in xref , 86.53% of lncRNA OTUD6B-AS1 expression was detected in the cytoplasm fraction in T24 bladder cancer cells, suggesting that the oxidative stress induced by lncRNA OTUD6B-AS1 may be achieved through regulation of miRNA."
32952848
sparser
"As shown in xref , miR-6734-5p was predicted to have three binding sites in lncRNA OTUD6B-AS1."
32952848
sparser
"Subsequently, we investigated the regulation of miR-6734-5p by lncRNA OTUD6B-AS1."
32952848
sparser
"The results revealed that miR-6734-5p levels were significantly increased, by more than 5.18-fold, in T24 cells overexpressing lncRNA OTUD6B-AS1 relative to untreated control cells ( P < 0.01) ( xref )."
32952848
sparser
"Nevertheless, we found that miR-6734-5p mimics did not significantly downregulate lncRNA OTUD6B-AS1 levels ( P > 0.05; xref )."
32952848
sparser
"These results demonstrate that lncRNA OTUD6B-AS1 functions to increase the miR-6734-5p level by enhancing miR-6734-5p stability."
32952848
sparser
"Further, lncRNA OTUD6B-AS1 significantly reduced IDH2 levels by 50% ( P < 0.01) (Figures xref and xref )."
32952848
sparser
"LncRNA OTUD6B-AS1 , which is induced by metal-regulatory transcription factor 1 (MTF1) after the treatment of arsenic trioxide (As 2 O 3 ), retains NRF2 in the cytoplasm to inhibit its function."
35625948
sparser
"On the other hand, OTUD6B-AS1 improves the stability of miR-6734-5p, a miRNA that targets isocitrate dehydrogenase 2 (IDH2) through direct binding to decrease the expression of IDH2 ."
35625948
sparser
"Furthermore, the elevation of Nrf2 in cytoplasm induced by lncRNA OTUD6B-AS1 was further significantly enhanced by miR-6734-5p; however, Nrf2 in the nucleus showed the opposite trend (Figures xref – xref )."
32952848
sparser
"These data indicate that miR-6734-5p contributes to oxidative stress as a downstream target of lncRNA OTUD6B-AS1."
32952848
sparser
"Together, these results demonstrate that miR-6734-5p serves as an intermediate in the regulation of IDH2 by lncRNA OTUD6B-AS1."
32952848
sparser
"We next characterized the interactions between lncRNA OTUD6B-AS1 and miR-6734-5p in evoking oxidative damage."
32952848
sparser
"lncRNA OTUD6B-AS1 can inhibit cancer cell proliferation [ xref ]; however, whether lncRNA OTUD6B-AS1 is also involved in oxidative damage triggered by As 2 O 3 in bladder cancer was previously unknown."
32952848
sparser
"Here, for the first time, we demonstrate that lncRNA OTUD6B-AS1 expression is upregulated by As 2 O 3 and that lncRNA OTUD6B-AS1 in regulated in response to oxidative stress."
32952848
sparser
"Further, we show that lncRNA OTUD6B-AS1 induction by As 2 O 3 is dependent on MTF1."
32952848
sparser
"As shown in Figures xref – xref , lncRNA OTUD6B-AS1 upregulated ROS and MDA and this activity could be significantly enhanced by miR-6734-5p."
32952848
sparser
"Further, upregulation of miR-6734-5p also significantly elevated the T24 cell apoptosis rate in response to lncRNA OTUD6B-AS1 overexpression, relative to lncRNA OTUD6B-AS1 overexpression alone ( P < 0.01) (Figures xref and xref )."
32952848
sparser
"The results of CCK-8 assays also revealed significantly lower cell viability of bladder cells overexpressing lncRNA OTUD6B-AS1 and miR-6734-5p mimics ( P < 0.01) ( xref )."
32952848
sparser
"Importantly, lncRNA OTUD6B-AS1 dramatically exacerbated As 2 O 3 -induced cytotoxicity by inducing oxidative stress."
32952848
sparser
"The novel lncRNA, OTUD6B-AS1, is an important target of gene expression [ xref ]."
32952848
sparser
"Data from The Cancer Genome Atlas database reveal that lncRNA OTUD6B-AS1 levels are reduced and that lncRNA OTUD6B-AS1 acts as an antioncogene in various tumors [ xref ]."
32952848
sparser
"More interestingly, another major finding was that the molecular mechanism involved lncRNA OTUD6B-AS1 inhibition of IDH2 expression through stabilization of miR-6734-5p ( xref )."
32952848
sparser
"Here, we found that lncRNA OTUD6B-AS1 transcription was activated by As 2 O 3 in bladder cancer cells, and these results were validated using a xenograft tumor model, suggesting that the antitumor effects of As 2 O 3 may be via upregulation of lncRNA OTUD6B-AS1; however, the roles of lncRNA OTUD6B-AS1 in exacerbated As 2 O 3 -induced oxidative damage have yet to be elucidated."
32952848
sparser
"Additionally, overexpression of lncRNA OTUD6B-AS1 significantly reduced tumor size and tumor weight in a xenograft model."
32952848
sparser
"Here, we investigated the function of lncRNA OTUD6B-AS1 and found that its overexpression clearly suppressed proliferation and elevated apoptosis of bladder cancer cells."
32952848
sparser
"These findings indicate that increased lncRNA OTUD6B-AS1 may be able to inhibit bladder cancer development, which is similar to the findings of Wang et al. [ xref ] that lncRNA OTUD6B-AS1 can suppress progression of clear cell renal cell carcinoma."
32952848
sparser
"Further, functional assays showed that overexpression of lncRNA OTUD6B-AS1 could exacerbate As 2 O 3 -induced cell damage both in vitro and in vivo ."
32952848
sparser
"Cumulatively, we believe that As 2 O 3 -induced cell damage is achieved by upregulation of lncRNA OTUD6B-AS1."
32952848
sparser
"Numerous studies have shown that ROS underlies the induction of cancer cell apoptosis by As 2 O 3 , and following lncRNA OTUD6B-AS1 overexpression, the elevation of ROS and MDA in T24 cells induced by As 2 O 3 was further significantly enhanced."
32952848
sparser
"Furthermore, the elevation of Nrf2 in cytoplasm induced by As 2 O 3 was further significantly enhanced by lncRNA OTUD6B-AS1; however, Nrf2 in the nucleus showed the opposite trend."
32952848
sparser
"Accordingly, we sought out to determine the mechanism through which MTF1 regulates lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Furthermore, we found that MTF1 knockdown eliminated the As 2 O 3 -induced increase of lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Collectively, these results offer new insights into the mechanisms involved in As 2 O 3 anticancer activity and demonstrate that lncRNA OTUD6B-AS1 expression can be activated by As 2 O 3 through ROS-mediated upregulation of MTF1."
32952848
sparser
"Furthermore, binding of MTF1 to the lncRNA OTUD6B-AS1 promoter sequence was directly proven by both ChIP and dual-luciferase reporter assays."
32952848
sparser
"We speculate that higher doses of As 2 O 3 may exceed the tolerance of metallothioneins and that lncRNA OTUD6B-AS1 induces MTF1 in response to uncleared As 2 O 3 , promoting ROS production and oxidative damage."
32952848
sparser
"Therefore, we speculated that lncRNA OTUD6B-AS1 might enhance oxidative stress by downregulating IDH2."
32952848
sparser
"The lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is another tumor-suppressive lncRNA that is downregulated in RCC patients and associated with lower survival rate and worse clinical outcomes."
36483915
sparser
"LncRNA OTUD6B-AS1 was found to be involved in the tumorigenesis and progression of various human cancers, including breast cancer [ 125–127 ]."
36202173
sparser
"Li et al. found that OTUD6B-AS1 binds and inhibits miR-26a-5p, which upregulates MTDH, thereby inducing autophagy and DNA damage in TNBC [ 128 ]."
36202173
sparser
"To our knowledge, no mechanism underlying the regulation of miRNA by lncRNA OTUD6B-AS1 has previously been reported."
32952848
sparser
"We found that lncRNA OTUD6B-AS1 expression was significantly higher in the cytoplasm fraction of T24 bladder cancer cells, supporting a role for lncRNA OTUD6B-AS1 function in regulation of miRNA."
32952848
sparser
"Ectopic expression of OTUD6B-AS1 inhibits tumor cell migration and invasion through suppressing the Wnt/β-catenin axis and reducing the expression of epithelial-to-mesenchymal transition (EMT)-related proteins including E-cadherin, N-cadherin, and Snail (Wang et al., 2019[ xref ])."
36483915
sparser
"Further, RNA-RNA pull-down assays revealed tight binding between lncRNA OTUD6B-AS1 and miR-6734-5p, indicating that lncRNA OTUD6B-AS1 may stabilize miR-6734-5p, preventing its degradation."
32952848
sparser
"Consequently, levels of miR-6734-5p were elevated in response to increased lncRNA OTUD6B-AS1 levels."
32952848
sparser
"These data indicate that lncRNA OTUD6B-AS1 can suppress IDH2 expression through stabilization of miR-6734-5p."
32952848
sparser
"These findings clearly suggest that the lncRNA OTUD6B-AS1 can be considered as a promising target for RCC treatment."
36483915
sparser
"Furthermore, miR-6734-5p can also inhibit development of colon cancer and acute myeloid leukemia [ xref , xref ]; however, the oxidative damage effects of lncRNA OTUD6B-AS1 and miR-6734-5p against bladder cancer have not previously been reported."
32952848
sparser
"Therefore, we sought to determine the interactions between lncRNA OTUD6B-AS1 and miR-6734-5p in evoking oxidative damage against bladder cancer."
32952848
sparser
"Intriguingly, gain-of-function experiments demonstrated the effects of the combination of lncRNA OTUD6B-AS1 and miR-6734-5p in promoting oxidative stress, and knockdown of IDH2 also had a similar effect."
32952848
sparser
"These results represent new data indicating how lncRNA OTUD6B-AS1 exacerbates As 2 O 3 -induced oxidative damage via miR-6734-5p-mediated functional inhibition of IDH2."
32952848
sparser
"Cytotoxicity analyses also indicated that both lncRNA OTUD6B-AS1 and miR-6734-5p, or both miR-6734-5p and knockdown of IDH2, clearly increased apoptosis rates relative to lncRNA OTUD6B-AS1, miR-6734-5p, or IDH2 knockdown alone."
32952848
sparser
"Overall, our results prove, for the first time, that the function of lncRNA OTUD6B-AS1 in enhancing As 2 O 3 -mediated oxidative damage is achieved by downregulation of IDH2 through stabilization of its negative regulator, miR-6734-5p."
32952848
sparser
"The regulation of lncRNA OTUD6B-AS1 expression by As 2 O 3 requires further exploration in a clinical context, to corroborate our findings."
32952848
sparser
"Additionally, further studies are needed to determine whether other As 2 O 3 -induced cytotoxic effects, including inhibition of cancer stem-like cells, are also mediated by lncRNA OTUD6B-AS1."
32952848
sparser
"In conclusion, the current study has elucidated a novel molecular mechanism of As 2 O 3 in evoking oxidative stress in bladder cancer, where MTF1 was increased by As 2 O 3 , thereby activating transcription of lncRNA OTUD6B-AS1."
32952848
sparser
"Subsequently, lncRNA OTUD6B-AS1 inhibited IDH2 expression by stabilizing miR-6734-5p, exacerbating As 2 O 3 -induced oxidative stress."
32952848
sparser
"In this study, we found that lncRNA TERC, lncRNA CCAT1 and lncRNA OTUD6B-AS1 were upregulated in CRC."
33493136
sparser
"The expression trend of lncRNA OTUD6B-AS1 in CRC and renal cell carcinoma is inconsistent, which may be related to tissue specificity and functional differences."
33493136
sparser
"LncRNA OTUD6B-AS1 is upregulated in clear cell renal cell cancer and enhances tumor proliferation [ xref ]."
33714208
sparser
"LncRNA ENST00000524003 (lncRNA OTUD6B-AS1) was downregulated in renal clear cells and inhibited the migration and invasion of cancer cells by inhibiting the activity of Wnt/ catenin pathway and epithelial-mesenchymal transformation [ xref ]."
33493136
sparser
"Long noncoding RNAs (lncRNAs) are loosely defined as RNAs that exceed 200 bases in length and have no apparent coding capacity, which have been shown to have important roles in promoting tumor formation and progression. [ xref , xref ] Currently, only few lncRNAs have recently been reported to be implicated in the modulation of autophagy in TNBC cells. [ xref ] For example, NAMPT‐AS is an oncogenic lncRNA in TNBC that epigenetically activates NAMPT to promote tumor progression and metastasis. [ xref ] Moreover, lncRNA OTUD6B‐AS1 promotes paclitaxel resistance in TNBC by regulation of miR‐26a‐5p/MTDH pathway‐mediated autophagy. [ xref ] As only a few autophagy‐associated lncRNAs were discovered in TNBC, the landscape of lncRNAs dysregulated and their molecular mechanisms in autophagic regulatory networks in TNBC remain largely unknown."
37096846
sparser
"lncRNA OTUD6B-AS1 could upregulate PNRC2 to inhibit the cellular processes in colorectal cancer xref ."
37024523
sparser
"The lncRNA OTUD6B-AS1 was also found to promote therapeutic resistance in TNBC, resulting in poor prognosis ( xref )."
37529698
sparser
"The lncRNAs YTHDF3-AS1, LINC00839, and OTUD6B-AS1 played an important role in promoting proliferation and metastasis of BC cells, according to our biofunctional experiments."
37529698
sparser
"This study focuses on the dysregulated expression of lncRNAs SNHG15 and OTUD6B-AS1 as potential biomarkers for disease progression and diagnostics."
40314733
sparser
"The findings from the bioinformatics analysis were substantiated by qPCR, which validated the differential expression of the lncRNAs SNHG15 and OTUD6B-AS1 in a larger cohort of samples, affirming their potential roles as biomarkers for SLE."
40314733
sparser
"The findings revealed significant up-regulation of SNHG15 (fold change = 2.01, p = 0.0011) and down-regulation of OTUD6B-AS1 (fold change = 0.26, p < 0.0001) in SLE patients compared to controls."
40314733
sparser
"In breast cancer, over-expression of OTUD6B‐AS1 has been found to promote autophagy and thus genomic instability via the lncRNA OTUD6B-AS1/miR-26a-5p/MTDH signalling pathway, this lncRNA promotes the progression of triple-negative breast cancer xref ."
37781080
sparser
"Correlation analyses linked OTUD6B-AS1 expression with hematological and inflammatory markers, while SNHG15 was associated with liver function indicators."
40314733
sparser
"ROC analysis demonstrated high diagnostic accuracy for both lncRNAs, particularly OTUD6B-AS1."
40314733
sparser
"This study establishes SNHG15 and OTUD6B-AS1 as promising biomarkers for SLE, highlighting their distinct associations with immune and organ-specific pathology."
40314733
sparser
"The robust diagnostic performance of OTUD6B-AS1, evidenced by its high sensitivity and specificity, suggests its utility in developing more accurate diagnostic tests for SLE."
40314733
sparser
"Using these HRLs, we finally constructed a novel prognostic model for patients with breast cancer, which consisted of 12 PHRLs: TDRKH-AS1, AC011978.2, AC110995.1, OTUD6B-AS1, YTHDF3-AS1, AL512380.1, MIR4435-2HG, HSD11B1-AS1, LINC02084, TRG-AS1, AL451085.3, and AL109955.1 (RBM38-AS1)."
34977036
sparser
"Zhang et al. documented that heightened expression of OTUD6B-AS1 led to the stabilization of TRIM16 by interacting with HuR, consequently augmenting GPX4-mediated ferroptosis and thereby mitigating radioresistance in CRC [ xref ]."
39036600
sparser
"Thyroid cancer growth and metastasis resulted strongly correlated with the expression of the lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) [ xref ]."
38586315
sparser
"According to Wang et al. (2020), OTUD6B-AS1 functions as a tumor suppressor in TC, and its overexpression inhibits TC cell viability, migration, and invasion [ xref ]."
38586315
sparser
"MiR-21 and miR-183-5p are both correlated with OTUD6B-AS1 activity, and their overexpression compromises the suppressive action of OTUD6B-AS1 on TC cell proliferation and invasiveness ( xref ) [ xref ]."
38586315
sparser
"Antitumorigenic effects have been reported for other lncRNAs such as LINC01510, Lnc ENST, and OTUD6B-AS1, playing a role via several signaling pathways or mediating miRNAs [ 319–321 ]."
35870535
sparser
"Lentivirus-mediated overexpression of OTUD6B-AS1 significantly reduces cell proliferation and promotes cell apoptosis in clear cell renal cell carcinoma (ccRCC), while OTUD6B-AS1 overexpression of β-catenin reduces the activity of the Wnt/β-catenin pathway and partially inhibits cell migration and invasion [ xref ]."
38880876
sparser
"Finally, OTUD6B also plays an important role in several diseases other than tumors; for example, biallelic variants in OTUD6B lead to mental retardation syndromes associated with seizures and dysmorphic features [ xref ]; OTUD6B-AS1 is also a key regulator of apoptosis in systemic sclerosis [ xref ]."
38880876
sparser
"OTUD6B-AS1 was demonstrated to indicate poor prognosis in ovarian cancer, clear cell renal cell carcinoma (ccRCC), and breast cancer (as an immune-related lncRNA) while its overexpression inhibited ccRCC proliferation ( xref ; xref ; xref )."
34977036
sparser
"Similarly, OTUD6B-AS1 played a tumor-suppressive role in thyroid carcinoma ( xref ), bladder carcinoma ( xref ), and CRC ( xref ; xref )."
34977036
sparser
"In contrast, in hepatocellular carcinoma (HCC), OTUD6B-AS1 could enhance cell proliferation and invasion ability via the GSKIP/Wnt/β-catenin signaling pathway ( xref )."
34977036
sparser
"LncRNA OTUD6B-AS1 induces autophagy and genome instability, increasing breast tumor progression and mediating paclitaxel resistance."
36780958
sparser
"LncRNA OTUD6B-AS1 sponges miR-26a-5p to increase MTDH expression in triggering autophagy to support the proliferation and survival of breast tumor cells and mediates resistance to paclitaxel chemother[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
36780958
sparser
"Although no functional affects were sought in this study [85] , one of the long noncoding RNAs found to be dysregulated was OTUD6B-AS1 and this has recently been shown to decrease Wnt pathway signalli[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30858032
sparser
"Long non-coding RNAs (lncRNAs) exert a key role in multiple cellular processes such as cell proliferation, migration and chemoresistance. xref , xref Du et al . found that lncRNA DLX6-AS1 enhanced cisplatin resistance in TNBC cells via miR-199b- 5p/PXN signaling. xref Li et al . showed that lncRNA OTUD6B-AS1 facilitate paclitaxel resistance in TNBC via miR-26a-5p/MTDH axis. xref Additionally, evidence has reported that lncRNAs play a key function in intracellular communication as well as in the tumor microenvironment. xref , xref "
36281649
sparser
"lncRNA OTUD6B-AS1 was associated with thyroid and renal carcinoma inhibition via regulation of Wnt/β-catenin signaling ( xref )."
38094755
sparser
"The main regulation mechanisms and results of the JAK/STAT signaling pathway include FUS-dependent JAK/STAT3 pathway activation (ITIH4-AS1), miR-18a/STAT3 sponging (CASC2), apoptosis induction via STAT5 and STAT6 (RP11-468E2.5), apoptosis induction via targeting STAT5/6 (RP11-468E2.5), CRC development via miR-9-5p/STMN1 and IL-6R regulation (lncRNA LINC01116), JAK/STAT3 pathway downregulation, expression of E-cadherin and occludin (lncRNA AB073614), cell proliferation and inhibition of apoptosis, JAK1/STAT3 signaling inhibition, Bcl-2 increase, caspase-3 and Bax downregulation (lncRNA LINC00346), miR-21-5p sponging, PNRC2 regulation, CRC progression inhibition (lncRNA OTUD6B-AS1), CRC development via miR-9-5p/STMN1 and IL-6R regulation (LINC01116), TPT1-mediated FAK and JAK-STAT3 signaling upregulation, CRC cell migration, miR-149-3p sponging, and CRC progression and metastasis (RNA TPT1-AS1 and RNA HOXA11-AS) ( xref )."
38094755
sparser
"Recently, various lncRNAs in the tumorigenesis of thyroid carcinoma have been well documented, such as LINC00673 ( xref ) and ZFAS1 ( xref ) which play an oncogenic role, while OTUD6B-AS1 ( xref ) and lncRNA-CTC function as tumor suppressor ( xref )."
35720005
sparser
"Other lncRNAs identified as potentially involved in cancer include FRMD6-AS1 [ xref , xref ] and LINC00466 [ xref , xref ], which have also been shown to promote tumor growth in vivo alongside [ xref ] LINC00877 [ xref ], LINC01091 [ xref ], NNT-AS1 [ xref , xref , xref ], ZNF252P-AS1 [ xref ], LZTS1-AS1 [ xref ], and OTUD6B-AS1 [ xref , xref ]."
37834450
sparser
"By contrast, lncRNA OTUD6B-AS1 possesses the function of inhibiting the cell viability, tumor migration, and tumor invasion of TC via miR-21 along with miR-183-5p ( xref )."
35720005
sparser
"Although several publications have indicated the occurrence of necroptosis regulators in KIRC, very few data are available on the involvement of lncRNAs in controlling or decreasing the neurotrophic signaling in kidney cancer, in particular based on OTUD6B-AS1, AL162377.1, AC108449.2, AF111167.2, and hsa-miR-21-5p targeting KLF9 [ xref – xref ]."
36226251
sparser
"Overall, these findings demonstrated that OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells via downregulation of miR-3171, suggesting that OTUD6B-AS1 may serve as a novel biomarker for CRC treatment."
33574932
sparser
"In human PASMCs and dermal fibroblasts, PDGF stimulation decreases the expression of lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1); and silencing this lncRNA decreases cell proliferation and apoptosis by targeting cyclin D1 [ xref ]."
39595622
sparser
"Overexpression of OTUD6B-AS1 promotes autophagy through lncRNA OtUD6B-AS1/miR-26a-5p/MTDH signalling pathway and thus affects the prognosis of breast cancer patients xref , xref ."
37781080
sparser
"13965984463825 + OTUD6B-AS1 * 0.741301698570473 + MORF4L2-AS1 * 3.51751820190694 + AC078846."
37781080
sparser
"LncRNA OTUD6B-AS1, MORF4L2-AS1 and LINC02649 were more highly expressed in the high-risk group, while AC024270.3, AC087392."
37781080
sparser
"1, OTUD6B-AS1 and MORF4L2-AS1 were correlated with the high-risk group."
37781080
sparser
"The role of OTUD6B-AS1 in different cancers varies: for example, over-expression of OTUD6B-AS1 in kidney cancer acts on the wnt/β-catenin pathway to inhibit cancer development and metastasis xref ; OTUD6B-AS1 is a biomarker of ovarian cancer occurrence and prognosis xref , and high expression of OTUD6B‐AS1 is closely associated with poor prognosis of ovarian cancer xref ."
37781080
sparser
"We calculated the risk scores for all samples using the following formula: Risk score = (0.309031851 × Exp of TDRKH-AS1) + (0.213481355 × Exp of AC011978.2) + (0.170772984 × Exp of AC110995.1) + (0.051734285 × Exp of OTUD6B-AS1) + (0.046646469 ×Exp of YTHDF3-AS1) + (0.019875191 × Exp of AL512380.1) + (0.019087737 × Exp of MIR4435-2HG) + (–0.037067764 × Exp of HSD11B1-AS1) + (–0.063799579 × Exp of LINC02084) + (–0.162152639 × Exp of TRG-AS1) + (–0.399031951 × Exp of AL451085.3) + (–0.886350422 × Exp of AL109955.1)."
34977036
sparser
"LncRNA OTUD6B-AS1 inhibits many cellular processes in colorectal cancer by sponging miR-21-5p and regulating PNRC2."
33686892
sparser
"Univariate Cox regression analysis was used for selecting six lncRNAs (NIFK-AS1, TP53TG1, TOLLIP-AS1, YTHDF3-AS1, LINC00839, and OTUD6B-AS1) that were associated with the prognosis of patients with BC ( xref ). xref depicts a forest map of the univariate Cox regression analysis."
37529698
sparser
"CCK-8 kit assay indicated that similar results as those of plate colony formation assay in MCF-7 cells were observed ( xref ), indicating that the lncRNAs YTHDF3-AS1, LINC00839, and OTUD6B-AS1 played an important role in promoting BC cell proliferation."
37529698
sparser
"In addition, transwell migration and invasion experiment showed that knocking down YTHDF3-AS1, LINC00839, and OTUD6B-AS1 led to decrease of invasive MCF-7 cells, but increased after silencing NIFK-AS1, TP53TG1, and TOLLIP-AS1 ( xref )."
37529698
sparser
"Therefore, we speculate that OTUD6B-AS1 plays a similar role in breast cancer and other cancers, and high OTUD6B-AS1 expression will be associated with the poor prognosis of breast cancer patients."
37781080
sparser
"MiKi et al. [ xref ] showed that Otud6b-AS1 silencing significantly reduced the proliferation and apoptosis of HPASMCs."
38878112
sparser
"Likewise, OTUD6B-AS1 may accelerate HCC cell proliferation and invasion by sequestering miR-664b-3p to induce GSKIP upregulation, which is associated with the activation of Wnt/β-catenin signaling [ xref ]."
37240232
sparser
"According to our data, OTUD6B-AS1 expression was low in CRC tissues and cells."
33686892
sparser
"LINC01871, LINC02384, LIPE-AS1, and HSD11B1-AS1 have been classified as low-risk ferroptosis-related lncRNAs (FRLncRNAs), while LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 are considered high-risk FRLncRNAs."
39763590
sparser
"Bioinformatic analysis and mechanistical experiments confirmed that OTUD6B-AS1 could act as a competitive endogenous RNA (ceRNA) to upregulate Proline-Rich Nuclear Receptor Coactivator 2 (PNRC2) expression by sequestering miR-21-5p."
33686892
sparser
"Additionally, the long noncoding RNA OTUD6B-AS1 (lncRNA OTUD6B-AS1) of OTUD6B exacerbates oxidative damage in bladder cancer [ xref ] and inhibits the growth, invasion, and migration of thyroid cancer [ xref ]."
34201062
sparser
"The lncRNA OTUD6B-AS1 also regulates the Wnt/β-catenin signaling pathway to induce the proliferation and invasion of hepatocellular carcinoma [ xref ]."
34201062
sparser
"Senescence‐associated gene set consisting of lncRNAs MCF2L‐AS1, USP30‐AS1, OTUD6B‐AS1, MAPT‐AS1, PRR34‐AS1 and DLGAP1‐AS1 can assess the benefits of chemotherapy and immunotherapy for breast cancer with early diagnostic utility [ xref ]."
39550706
sparser
"LncRNA OTUD6B-AS1 overexpression promoted GPX4-mediated ferroptosis to suppress radioresistance in colorectal cancer."
37184781
sparser
"Overexpression of OTUD6B-AS1 significantly decreased the viability, migration, and invasion of thyroid carcinoma cells."
32256450
sparser
"We also confirmed the effects of OTUD6B-AS1 on ccRCC cells."
30670025
sparser
"The overexpression of OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of epithelial-to-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin and Snail) in ccRCC cells."
30670025
sparser
"Ferroptosis inhibitor attenuated radioresistance (P < 0.01) caused by lncRNA OTUD6B-AS1 overexpression."
37184781
sparser
"LncRNA OTUD6B-AS1 stabilized TRIM16 mRNA via binding to HuR."
37184781
sparser
"OTUD6B-AS1 overexpression stabilized TRIM16 via binding to HuR and increased GPX4-mediated ferroptosis, thus attenuating CRC radioresistance."
37184781
sparser
"Our constructed prognostic model, derived from lncRNAs linked to ARGs, comprised 11 significant lncRNAs: NRAV, MCM3AP-AS1, OTUD6B-AS1, AC026356.1, AC009133.1, DDX11-AS1, AC108463.2, MIR4435-2HG, WARS2-AS1, LINC01094, and HCG18."
38824386
sparser
"Eighteen cuproptosis-related lncRNAs were identified and 11 of them including AL023882.1 , AC091588.1 , AC138028.2 , AC027514.1 , AL592301.1 , LRRC8C-DT , MFF-DT , NIFK-AS1 , MECOM-AS1 , OTUD6B-AS1 and RNF32-AS1 were selected for risk score system construction."
37434691
sparser
"From these 33 lncRNAs, we selected 11 lncRNAs (NRAV, MCM3AP-AS1, OTUD6B-AS1, AC026356.1, AC009133.1, DDX11-AS1, AC108463.2, MIR4435-2HG, WARS2-AS1, LINC01094, and HCG18) and constructed a COX risk model."
38824386
sparser
"LncRNA OTUD6B-AS1 suppresses the progression and viability of colorectal tumor cells and stimulates apoptosis."
36423787
sparser
"LncRNA OTUD6B-AS1 sponges miR-21–5p which increases the expression levels of PNRC2 and in turn induces apoptosis to interfere with colorectal tumor progression [198] ."
36423787
sparser
"In this study, we constructed a risk model for the ARG-related lncRNAs NRAV, MCM3AP-AS1, OTUD6B-AS1, AC026356.1, AC009133.1, DDX11-AS1, AC108463.2, MIR4435-2HG, WARS2-AS1, LINC01094, and HCG18 and examined the relationship between the model and the prognosis and immune cells of patients with HCC."
38824386
sparser
"lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is a tumor-suppressive noncoding RNA in clear cell renal cell carcinoma."
32256450
sparser
"The long noncoding RNA (lncRNA) OTUD6B antisense RNA 1 (OTUD6B-AS1) is oriented in an antisense direction to the protein-coding gene OTUD6B on the opposite DNA strand."
30670025
sparser
"TCGA database data show that the expression of the lncRNA OTUD6B-AS1 is downregulated and that OTUD6B-AS1 acts as an antioncogene in a variety of tumors."
30670025
sparser
"However, the expression and biological functions of the lncRNA OTUD6B-AS1 are still unknown in tumors, including clear cell renal cell carcinoma (ccRCC)."
30670025
sparser
"The role of OTUD6B-AS1 in thyroid carcinomas has not been reported yet."
32256450
sparser
"We aim to investigate the expression and biological functions of OTUD6B-AS1 in thyroid carcinomas."
32256450
sparser
"The expression level of OTUD6B-AS1 was measured in 75 paired human ccRCC tissue and corresponding adjacent normal renal tissue samples."
30670025
sparser
"The correlations between the OTUD6B-AS1 expression level and clinicopathological features were evaluated using the chi-square test."
30670025
sparser
"Methods: The expression level of OTUD6B-AS1 was measured in 60 paired human thyroid carcinoma tissues and corresponding adjacent normal thyroid tissues."
32256450
sparser
"The effects of OTUD6B-AS1 on ccRCC cells were determined via MTT assay, clone formation assay, transwell assay, and flow cytometry."
30670025
sparser
"Furthermore, the impact of OTUD6B-AS1 overexpression on the activation of the Wnt/β-catenin signaling pathway was investigated."
30670025
sparser
"The aim of the present study was to investigate the role of ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) in CRC and its underlying mechanisms."
33574932
sparser
"The correlations between the OTUD6B-AS1 expression levels and clinicopathological features were evaluated using the Mann-Whitney test."
32256450
sparser
"Finally, ACHN cells with OTUD6B-AS1 overexpression were subcutaneously injected into nude mice to evaluate the influence of OTUD6B-AS1 on tumor growth in vivo."
30670025
sparser
"OTUD6B-AS1 inhibits viability, migration, and invasion of thyroid carcinoma by targeting miR-183-5p and miR-21."
32256450
sparser
"Taken together, our findings present a road map for targeting the newly identified lncRNA OTUD6B-AS1 to suppress ccRCC progression in cell lines, and these results elucidate a novel potential therapeutic target for ccRCC treatment."
30670025
sparser
"As for our research, we are the first to report the expression pattern, biological function and potential regulatory mechanism of OTUD6B-AS1 in ccRCC."
30670025
sparser
"These data indicated that miR-21 and miR-183-5p are bound to OTUD6B-AS1."
32256450
sparser
"Results: OTUD6B-AS1 was downregulated in thyroid carcinoma tissue samples."
32256450
sparser
"The Wnt/β-catenin pathway is evolutionarily well-conserved, and aberrant activation of this pathway can cause abnormal cell growth and malignant transformation ( xref ; xref ). xref revealed that the novel lncRNA OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
33178684
sparser
"This study explored the mechanism of long non-coding RNA ovarian tumor domain containing 6B-antisense RNA1 (lncRNA OTUD6B-AS1) in CRC radioresistance through tripartite motif 16 (TRIM16)."
37184781
sparser
"The subcellular localization of lncRNA OTUD6B-AS1 was tested by the nuclear/cytoplasmic fractionation assay, with RNA immunoprecipitation assay to validate gene interactions."
37184781
sparser
"LncRNA OTUD6B-AS1 and TRIM16 were poorly expressed (P < 0.01) in CRC tissues and cells and further decreased (P < 0.01) in radioresistant CRC cells."
37184781
sparser
"OTUD6B-AS1 overexpression decreased cell survival (P < 0.01), increased γ-H2AX levels (P < 0.01), and elevated ferroptosis and oxidative stress (P < 0.01) after X-ray radiation."
37184781
sparser
"Quantitative real-time PCR (qRT-PCR) assays revealed that OTUD6B-AS1 expression was significantly decreased in ccRCC tissue samples and cell lines, suggesting antioncogene functions for OTUD6B-AS1."
30670025
sparser
"OTUD6B-AS1 expression in CRC cell lines was examined using reverse transcription-quantitative PCR."
33574932
sparser
"In this study, we found that the expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue samples and that patients with low OTUD6B-AS1 expression had shorter overall survival than patients with high OTUD6B-AS1 expression, which showed that the different expression level of OTUD6B-AS1 indirectly correlated with survival of patients."
30670025
sparser
"Lentivirus-mediated OTUD6B-AS1 overexpression significantly decreased the proliferation of ccRCC cells and promoted the apoptosis of the cells."
30670025
sparser
"The effects of OTUD6B-AS1 on thyroid carcinoma cells were determined via the MTT and transwell assays."
32256450
sparser
"Furthermore, OTUD6B-AS1 overexpression partly inhibited cell migration and invasion."
30670025
sparser
"Luciferase reporter assay was used to confirm the interactions between OTUD6B-AS1 and its potential targets."
32256450
sparser
"The potential targets of OTUD6B-AS1 were screened using the online programs OncomiR and StarBase 3.0, and the LncBase Predicted v."
32256450
sparser
"The expression of OTUD6B-AS1 correlated with tumor size, clinical stage, and lymphatic metastasis of thyroid carcinoma."
32256450
sparser
"The probable relationship between OTUD6B-AS1 and overall survival in ccRCC patients were also determined."
30670025
sparser
"Furthermore, our findings indicated that the lncRNA OTUD6B-AS1 inhibited ccRCC proliferation through the inactivation of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
30670025
sparser
"Comprehensive analysis of the lncRNA OTUD6B-AS1 in the TCGA database."
30670025
sparser
"Likewise, OTUD6B-AS1 was classified as a ferroptosis-related risk lncRNA in BC survival ( xref )."
36189319
sparser
"After the LASSO regression, 11 of them, including AL023882.1, AC091588.1, AC138028.2, AC027514.1, AL592301.1, LRRC8C-DT, MFF-DT, NIFK-AS1, MECOM-AS1, OTUD6B-AS1, and RNF32-AS1 were selected to develop a risk score system, which was subsequently proven to have an independent prognostic value."
37434691
sparser
"In the kidney clear cell carcinoma (KIRC) cohort, the patients in the high OTUD6B-AS1 expression group had a five-year survival rate 22.8% higher than the five-year survival rate of 15.2% of the patients in the low OTUD6B-AS1 expression group."
30670025
sparser
"Online programs predicted miR-183-5p and miR-21 as potential targets of OTUD6B-AS1."
32256450
sparser
"The overall survival status significantly differed between the high OTUD6B-AS1 expression group and the low OTUD6B-AS1 expression group (HR = 1.962, 95% CI 1.445–2.664, p  < 0.0001; Fig. xref b)."
30670025
sparser
"OTUD6B-AS1 (ovarian tumor domain containing 6B antisense RNA1) was reported to be related to the cell death of cancers including bladder cancer, clear renal cell cancer, and breast cancer ( xref )."
37434691
sparser
"Therefore, OTUD6B-AS1 could be used as an indicator of ccRCC."
30670025
sparser
"However, no significant difference in overall survival status was found between the high OTUD6B-AS1 expression group and the low OTUD6B-AS1 expression group in the KIRP and KICH cohorts (Fig. xref c and d)."
30670025
sparser
"Then, Kaplan-Meier analysis was used to evaluate the relationship between OTUD6B-AS1 expression in ccRCC and patient clinicopathological characteristics, and the results showed that the survival time of the patients with pathologic stage I + II disease ( n  = 318) was longer than that of the patients with advanced stage lesions ( n  = 205) ( P  < 0.0001, Additional file xref : Figure S1A)."
30670025
sparser
"Li et al. reported in their study that OTUD6B-AS1 could promote paclitaxel resistance of triple-negative breast cancers by mediating autophagy and genomic instability ( xref )."
37434691
sparser
"Luciferase reporter assays showed miR-183-5p and miR-21 bound to OTUD6B-AS1."
32256450
sparser
"Xu et al. found that OTUD6B-AS1 was related to ferroptosis and able to influence the immune microenvironment in breast cancer ( xref )."
37434691
sparser
"The expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue compared with normal tissue and was associated with clinicopathological features of patients with ccRCC."
30670025
sparser
"OTUD6B-AS1 knockdown significantly reduced proliferation in both dermal fibroblasts and HPASMCs by increasing target cyclin D1 expression at the mRNA and protein levels."
35464474
sparser
"In addition, OTUD6B-AS1 knockdown favored cells apoptosis resistance, which can be regarded as a compensatory mechanism for inhibition of cells proliferation."
35464474
sparser
"Moreover, overexpression of miR-183-5p and miR-21 compromised the inhibitory effects of OTUD6B-AS1 on viability, migration, and invasion of thyroid carcinoma cells."
32256450
sparser
"To explore the role of the lncRNA OTUD6B-AS1 in ccRCC, we analyzed the expression of OTUD6B-AS1 in a cohort of 75 ccRCC tissue samples and matched nontumor samples using qRT-PCR analysis, with our results showing that OTUD6B-AS1 expression was remarkably decreased in the ccRCC tissue samples compared with the paired adjacent normal tissue samples (Fig.  xref a and b)."
30670025
sparser
"Therefore, downregulated OTUD6B-AS1 resulted in cell dysregulation in SSc-related fibrosis and microangiopathy ( xref )."
35464474
sparser
"Conclusions: Taken together, our findings present in vitro evidence of lncRNA OTUD6B-AS1 as a tumor suppressor in thyroid carcinomas."
32256450
sparser
"We also found that the expression of OTUD6B-AS1 was related to the pathologic tumor stage, clinical grade, lymph node status and metastasis status but not the gender and age of patients (Fig. xref c and Table  xref )."
30670025
sparser
"BC cases were divided into high-risk and low-risk groups based on the median risk score, calculated by 8 GT-lncRNAs, including MIR4435-2HG, MAPT-AS1, TGFB2-AS1, AL357054.4, AL161719.1, OTUD6B-AS1, AC083799.1, and LINC01016."
36189319
sparser
"Wang et al. reported in their study that OTUD6B-AS1 could promote apoptosis of clear renal cell carcinoma through the Wnt/β-catenin pathway ( xref )."
37434691
sparser
"Then, we included the variables age, gender, clinical tumor grade, pathologic stage, lymph node status, metastasis status, and OTUD6B-AS1 mRNA level in a multivariate Cox regression model and found that the OTUD6B-AS1 mRNA level was an independent predictor for overall survival status in KIRC patients (HR = 0.275, 95% CI 0.081–0.930, p  = 0.038; Table  xref )."
30670025
sparser
"OTUD6B-AS1 is a newly identified lncRNA, whose overexpression could suppress tumor growth in kidney cancer, thyroid cancer, and colorectal cancer, but boost tumor growth in hepatocellular carcinoma ( xref )."
36189319
sparser
"Ma et al. reported OTUD6B-AS1 as an immune-related lncRNA and served as a risk factor in BC patient prognosis ( xref )."
36189319
sparser
"To assess whether OTUD6B-AS1 could be used as an indicator for the diagnosis of ccRCC, an ROC curve was built."
30670025
sparser
"Univariate Cox analysis ( p < 0.05) was utilized to choose 81 differentially expressed prognostic-related lncRNAs: THBS4-AS1, LINC01711, MACORIS, KIAA1671-AS1, BACE1-AS, SIAH2-AS1, LINC00571, RAP2C-AS1, ARF4-AS1, MYOSLID, PLBD1-AS1, FALEC, GNG12-AS1, AGAP2-AS1, OXCT1-AS1, FOXD2-AS1, SNHG9, LINC00882, APCDD1L-DT, SNHG11, OXCT1-AS1, CTBP1-DT, HHLA3, NNT-AS1, MAP3K4-AS1, OIP5-AS1, LINC01671, LASTR, NFE4, GTF3C2-AS1, LINC01801, LINC00886, CDK6-AS1, EIF3J-DT, MHENCR, LINC01605, H1-10-AS1, SBF2-AS1, PCCA-DT, LYPLAL1-DT, COLCA1, SNHG3, GAS6-DT, LINC02027, SGMS1-AS1, BDNF-AS, KLHL7-DT, NORAD, DHRS4-AS1, SNHG15, LHFPL3-AS2, LINC00460, LINC02446, LINC02195, LINC00271, GATA2-AS1, LINC01011, SEPTIN7-DT, SNHG8, UGDH-AS1, CYTOR, MANCR, MIR4435-2HG, ITGA9-AS1, ZBTB20-AS4, SUCLG2-AS1, LINC01507, OTUD6B-AS1, EIF1B- AS1, HCG25, PAXIP1-AS2, WDFY3-AS2, TGFB2-AS1, BAALC-AS1, LINC00941, LINC02154, SNHG6, EMS2OS, MINCR, ATP1A1-AS1, LINC00623, and LINC01415 ( xref )."
35910212
sparser
"Therefore, it was hypothesized that OTUD6B-AS1 could exert certain effects on the proliferation, invasion and migration of CRC cells by regulating miR-3171."
33574932
sparser
"OTUD6B-AS1 was initially reported as active in fibrosis in systemic sclerosis ( xref )."
32256450
sparser
"In this study, we selected ACHN and OS-RC-2 cells as they had the lowest OTUD6B-AS1 expression among the ccRCC cell lines (Fig.  xref a)."
30670025
sparser
"In this section, we evaluated the effect of a DNA demethylating agent (5-Aza-CdR) on OTUD6B-AS1 expression at the cellular level."
30670025
sparser
"The role of OTUD6B-AS1 in cancer is largely unknown."
32256450
sparser
"More notably, through the comprehensive analysis of heatmap, we regarded LINC01871, LINC02384, LIPE-AS1, and HSD11B1-AS1 as protective LncRNAs, while LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were classified as risk LncRNAs."
34671639
sparser
"A total of 14 lncRNAs were identified as protective genes (AC022150.4, AC061992.1, AC090948.3, AC092794.1, AC107464.3, AL021707.8, AL451085.2, AL606834.2, FLJ42351, LINC00926, LINC01871, TNFRSF14−AS1, U73166.1 and USP30−AS1) with HRs < 1 while 10 lncRNAs (AC022150.2, AC090948.1, AC243960.1, AL021707.6, ITGB2−AS1, OTUD6B−AS1, SP2−AS1, TOLLIP−AS1, Z68871.1 and ZNF337−AS1) were associated with increased risk with HRs >1."
35441740
sparser
"First, we found that the OTUD6B-AS1 promoter was methylated by consulting the UCSC database ()."
30670025
sparser
"A recent study reveals the role of OTUD6B-AS1 in clear cell renal cell carcinoma (ccRCC) ( xref )."
32256450
sparser
"Following the treatment of ACHN and OS-RC-2 cells with 5-Aza-CdR, the expression level of OTUD6B-AS1 was significantly higher in the 5-Aza-CdR-treated cells than in the control cells (Fig. xref b)."
30670025
sparser
"As predicted by TCGA, OTUD6B-AS1 expression was markedly downregulated in CRC tissues compared with in normal tissues ( xref )."
33574932
sparser
"In addition, it has been reported that the overexpression of OTUD6B-AS1 makes hepatocellular carcinoma cells more aggressive through the GSKIP/Wnt/β-catenin signaling pathway ( xref )."
34671639
sparser
"Overexpression of OTUD6B-AS1 markedly suppressed the proliferation of ccRCC cells in vitro."
30670025
sparser
"This study demonstrates that the lower expression of OTUD6B-AS1 is associated with poor prognosis of ccRCC ( xref )."
32256450
sparser
"Then, OTUD6B-AS1 was overexpressed in ACHN and OS-RC-2 cells transfected with the plvx-OTUD6B-AS1."
30670025
sparser
"OTUD6B‐AS1 may act different roles in different cancers."
35441740
sparser
"OTUD6B‐AS1 suppresses viability, migration and invasion in thyroid carcinomas, colorectal cancer cell and renal cell carcinoma. xref , xref , xref , xref On the other hand, OTUD6B‐AS1 promotes hepatocellular carcinoma cells proliferation and invasion and induces chemoresistance in breast cancer cell and cervical cancer cell. xref , xref , xref How to make a wide use of OTUD6B‐AS1 is worth exploring and may provide a novel strategy to cancer treatment."
35441740
sparser
"qRT-PCR analysis was performed at 48 h posttransfection, and the data revealed that OTUD6B-AS1 expression was significantly increased by plvx-OTUD6B-AS1 compared with the empty vector. (Fig. xref c)."
30670025
sparser
"On the contrary, LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were highly expressed in the high-risk groups, demonstrating that these FRLncRNAs were high-risk LncRNAs."
34671639
sparser
"Additionally, 12-FRLncRNA signature involved with LINC01871, LINC00393, AC121247.2, LINC02384, LIPE-AS1, HSD11B1-AS1, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 could accurately predict the prognosis of patients with BC, which was confirmed by the training set, validation set, and set from GEO database."
34671639
sparser
"To identify the function of OTUD6B-AS1 in ccRCC, we performed gain-of-function assays."
30670025
sparser
"Furthermore, the expression levels of OTUD6B-AS1 were assessed in different CRC cell lines and in normal HIEC cells."
33574932
sparser
"Compared with those in normal HIEC cells, the expression levels of OTUD6B-AS1 in CRC cell lines (Caco2, HCT116, LoVo, SW480 and SNU-C1) were markedly downregulated, particularly in HCT116 cells ( xref )."
33574932
sparser
"Similarly, increased OTUD6B-AS1 expression impaired the colony formation capacities of ccRCC cells (Fig. xref f and g)."
30670025
sparser
"According to the comprehensive analysis of 12-FRLncRNA signature, LINC01871, LINC02384, LIPE-AS1, and HSD11B1-AS1 were low-risk FRLncRNAs, whereas LINC00393, AC121247.2, AC010655.2, LINC01419, PTPRD-AS1, AC099329.2, OTUD6B-AS1, and LINC02266 were high-risk FRLncRNAs."
34671639
sparser
"For instance, lncRNA OTUD6B-AS1 was highly expressed in HCC and associated with HCC patients' prognosis."
34594378
sparser
"These findings were confirmed by the results of ki-67 staining assays (Fig. xref h) and highlighted OTUD6B-AS1 as an antioncogene in ccRCC cells."
30670025
sparser
"Moreover, silencing OTUD6B-AS1 inhibited HCC proliferation and invasion through the Wnt/ β -catenin pathway [ xref ]."
34594378
sparser
"However, the expressions and biological functions of OTUD6B-AS1 are still unknown in thyroid carcinomas."
32256450
sparser
"Furthermore, we investigated the effect of OTUD6B-AS1 on cell migration by performing a transwell assay without a Matrigel coating in the upper compartment."
30670025
sparser
"Upregulation of OTUD6B-AS1 expression induces ccRCC cell apoptosis and alters cell cycle progression in vitro."
30670025
sparser
"Currently, no OTUD6B-AS1 that act as ceRNAs have been reported in thyroid carcinomas."
32256450
sparser
"After constructing the overexpression plasmid of OTUD6B-AS1, transfection efficiency was verified by RT-qPCR, and it was demonstrated that OTUD6B-AS1 expression was significantly upregulated in the Oe-OTUD6B-AS1 group compared with the Oe-NC group ( xref )."
33574932
sparser
"OTUD6B-AS1 overexpression inhibited the migration and invasion of ccRCC cells in vitro."
30670025
sparser
"Additionally, an immunofluorescence assay revealed a decrease in the expression levels of proliferation-related protein Ki67 following OTUD6B-AS1 overexpression ( xref )."
33574932
sparser
"Next, we studied whether OTUD6B-AS1 could affect the migration and invasion of ccRCC cells."
30670025
sparser
"The results showed that the invasion of ACHN (upper) and OS-RC-2 (lower) cells was notably decreased with OTUD6B-AS1 overexpression (Fig.  xref a and b)."
30670025
sparser
"In vitro and in vivo evidence indicates that OTUD6B-AS1 functions as a tumor suppressor in ccRCC ( xref )."
32256450
sparser
"To evaluate the impact of OTUD6B-AS1 on the ccRCC cell cycle and apoptosis, flow cytometry assays and Hoechst staining analysis were conducted."
30670025
sparser
"Additionally, western blot assays revealed remarkable alterations in CCNB1 and CCND1 expressing in the ACHN and OS-RC-2 cells overexpressing OTUD6B-AS1, confirming that OTUD6B-AS1 is involved in cell cycle regulation (Fig. xref c)."
30670025
sparser
"In this work, we investigated the expression of OTUD6B-AS1 in human thyroid carcinoma tissues and corresponding adjacent normal thyroid tissue samples."
32256450
sparser
"Moreover, the ACHN and OS-RC-2 cells transfected with the OTUD6B-AS1 overexpression plasmid exhibited higher levels of apoptosis than the corresponding control cells (Fig. xref d and e)."
30670025
sparser
"As expected, the results of the RT-qPCR assay ( xref ) indicated that miR-3171 expression was markedly reduced following OTUD6B-AS1 overexpression compared with that in the OE-NC group."
33574932
sparser
"We also evaluated the correlations between the expression levels of OTUD6B-AS1 and the clinicopathological parameters."
32256450
sparser
"Furthermore, the protein expression levels of cleaved PARP and Bax exhibited significant increases, while Bcl-2 gene expression exhibited significant decreases in the ACHN and OS-RC-2 transfected with the OTUD6B-AS1 overexpression plasmid (Fig. xref f)."
30670025
sparser
"The qRT-PCR results showed that OTUD6B-AS1 expression was significantly enhanced in the SW579 and TPC-1 cells after transfection with OTUD6B-AS1- pcDNA3.1 compared to transfection with empty pcDNA3.1 ( xref )."
32256450
sparser
"We investigated the consequences of OTUD6B-AS1 overexpression on the activation of the Wnt/β-catenin pathway in ccRCC cells."
30670025
sparser
"Moreover, we screened the potential targets of OTUD6B-AS1 and explored the ceRNAs molecular mechanisms underlying the regulatory effects of OTUD6B-AS1 on thyroid carcinoma cells."
32256450
sparser
"These data suggest that OTUD6B-AS1 overexpression can promote G1/G0 arrest, inhibit G2/M phase and increase the apoptotic rate of ccRCC cells in vitro."
30670025
sparser
"Overexpression of OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins."
30670025
sparser
"OTUD6B-AS1 Is Downregulated in Thyroid Carcinoma."
32256450
sparser
"We investigated the expression of OTUD6B-AS1 in 60 pair of thyroid carcinoma tissues and adjacent para-cancerous tissues."
32256450
sparser
"We investigated the possible pathways that contribute to the effects of OTUD6B-AS1 on ccRCC."
30670025
sparser
"To test the effect of OTUD6B-AS1 overexpression on the Wnt/β-catenin signaling pathway, we analyzed the expression of several key proteins of this signaling pathway by western blotting."
30670025
sparser
"We found that OTUD6B-AS1 was downregulated in the thyroid carcinoma tissues compared to the para-cancerous tissues ( p < 0.01, xref )."
32256450
sparser
"Compared with the control group, the OTUD6B-AS1 overexpression group exhibited decreases in both phosphorylated and nonphosphorylated AKT and glycogen synthase kinase 3β (GSK3β), and the total level of β-catenin was also decreased in both ACHN and OS-RC-2 cells (Fig. xref b)."
30670025
sparser
"The correlations between the OTUD6B-AS1 expression level and the clinicopathological features were evaluated using the Mann-Whitney test."
32256450
sparser
"Additionally, to demonstrate the mechanism of OTUD6B-AS1 acting as an antioncogene in ccRCC cells, the expression of EMT-related proteins was determined by western blotting."
30670025
sparser
"The overexpression of OTUD6B-AS1 inhibited the proliferation, migration and invasion of ccRCC cells in vitro and in vivo and promoted apoptosis in ccRCC cells."
30670025
sparser
"In this study, we identified that the expression of the lncRNA OTUD6B-AS1 was downregulated in ccRCC tissue compared with matched paracancerous tissue, and the downregulation of OTUD6B-AS1 expression was associated with worse clinical characteristics and poor overall survival."
30670025
sparser
"The upregulation of OTUD6B-AS1 expression significantly upregulated the expression of the epithelial marker E-cadherin, while the expression levels of the mesenchymal cell markers N-cadherin and Snail were downregulated in both the ACHN and OS-RC-2 cells with enhanced expression of OTUD6B-AS1 (Fig. xref c)."
30670025
sparser
"No correlation was observed between expression of OTUD6B-AS1 and age ( p = 0.57, xref ), gender ( p = 0.18, xref ), and multifocality ( p = 0.57, xref )."
32256450
sparser
"However, when we knocked down OTUD6B-AS1 expression in 786-O cells, we observed the opposite result."
30670025
sparser
"Based on the results above, we can infer that OTUD6B-AS1 can regulate the activity of the Wnt/β-catenin signaling pathway and affect the expression of several EMT-related proteins in ccRCC cells."
30670025
sparser
"Interestingly, the downregulation of OTUD6B-AS1 inhibited PTX-induced autophagy, while the upregulation of OTUD6B-AS1 and MTDH inhibited DNA damage response (DDR), promoted the autophagy process by the upregulation of LC3B-II and decreased PTX-mediated cell death [ xref ]."
37958880
sparser
"Thus, the overexpression of H19, DDX11-AS1, OTUD6B-AS1, and HOTAIR induces drug resistance in BC cells by promoting autophagy."
37958880
sparser
"OTUD6B-AS1 inhibits tumorigenesis in ccRCC cells in vivo."
30670025
sparser
"Furthermore, miR-3171 was demonstrated to be a direct target of OTUD6B-AS1 using a luciferase reporter assay."
33574932
sparser
"OTUD6B-AS1 correlated with tumor size ( p = 0.013, xref ), lymphatic metastasis ( p = 0.015, xref ), and clinical stage ( p = 0.0213 xref )."
32256450
sparser
"To investigate the impact of OTUD6B-AS1 in vivo, we inoculated empty vector- or plvx-OTUD6B-AS1-transfected ACHN cells into nude mice."
30670025
sparser
"Besides, recent accumulating evidence has indicated that lncRNAs, such as OTUD6B-AS1, URRCC, HOTAIRM1 and MRCCAT1, play important regulatory roles in diverse biological processes in ccRCC xref - xref ."
33854630
sparser
"A prognostic risk model comprised of six m 6 A-regulated lncRNAs-Z68871.1, AL122010.1, AL138724.1,OTUD6B-AS1, AC090948.3 and eosinophil granule ontogeny transcript (EGOT) for high-risk patients with tumor-infiltrating immune cells, indicated that m 6 A-regulated lncRNAs may modulate the immune microenvironment in breast cancer ( xref )."
35721510
sparser
"Then, qRT-PCR experiments determined an obvious increase in OTUD6B-AS1 expression in the tumor tissue derived from the plvx-OTUD6B-AS1-transfected ACHN cells compared with that derived from the respective control cells (Fig.  xref d)."
30670025
sparser
"OTUD6B-AS1 Inhibits Migration and Invasion of Thyroid Carcinoma."
32256450
sparser
"These findings indicate that OTUD6B-AS1 upregulation suppresses ccRCC tumor cell growth in vivo."
30670025
sparser
"We first examined the expression of OTUD6B-AS1 in thyroid carcinoma cells SW579 and TPC-1, and normal thyroid cells Nthy-ori."
32256450
sparser
"The rescue assays revealed that miR-3171 mimics markedly reversed the inhibitory effects of OTUD6B-AS1 overexpression on proliferation, invasion and migration of CRC cells."
33574932
sparser
"The lncRNA OTUD6B-AS1 is oriented in an antisense direction to the protein-coding gene OTUD6B on the opposite DNA strand."
30670025
sparser
"Compared to the normal thyroid cells Nthy-ori, decreased expression of OTUD6B-AS1 was observed in the SW579 and TPC-1 cells ( p < 0.001, xref )."
32256450
sparser
"Although previous studies have shown that OTUD6B-AS1 expression is deregulated in the skin tissue of patients with systemic sclerosis [ xref ], the mechanism of OTUD6B-AS1 in tumors and other diseases, especially RCC, is unknown."
30670025
sparser
"To elucidate the role of OTUD6B-AS1 in thyroid carcinoma, SW579 and TPC-1 cells were transfected with OTUD6B-AS1–pcDNA3.1 to overexpress OTUD6B-AS1."
32256450
sparser
"Collectively, our results demonstrated that OTUD6B-AS1, acting as an antioncogene, may be a potential diagnostic and prognostic biomarker as well as a therapeutic target in ccRCC."
30670025
sparser
"Transwell migration and invasion assays showed that SW579 and TPC-1 cells overexpressed OTUD6B-AS1 decreased migration and invasion abilities ( xref )."
32256450
sparser
"As mentioned above, we explored the mechanism by which OTUD6B-AS1 overexpression affects ccRCC by performing experiments to demonstrate that OTUD6B-AS1 decreased the activity of the Wnt/β-catenin pathway and suppressed the expression of EMT-related proteins in ccRCC cells."
30670025
sparser
"In the current work, we evaluated the function of OTUD6B-AS1 in ccRCC progression and found a negative correlation between ccRCC malignancy and OTUD6B-AS1 expression."
30670025
sparser
"Furthermore, The Cancer Genome Atlas database was utilized to examine the expression levels of OTUD6B-AS1 in CRC tissues."
33574932
sparser
"Following OTUD6B-AS1 overexpression, Cell Counting Kit-8 and colony formation assays were used to detect the proliferation ability of HCT116 cells."
33574932
sparser
"Overexpression of OTUD6B-AS1 inhibited the proliferation, invasion and migration of HCT116 cells."
33574932
sparser
"Additionally, a luciferase reporter assay was used to verify the potential interaction between OTUD6B-AS1 and microRNA-3171 (miR-3171)."
33574932
sparser
"Subsequently, rescue assays were performed to clarify the regulatory effects of OTUD6B-AS1 and miR-3171 on CRC development."
33574932
sparser
"miR-3171 is a direct target of OTUD6B-AS1."
33574932
sparser
"The results demonstrated that OTUD6B-AS1 expression was low in CRC cells and tissues."
33574932
sparser
"In addition our data were only obtained with cell lines, and that cell line models have several limitations,so there’s still a lot of work to be done to study OTUD6B-AS1 in depth."
30670025
sparser
"The risk score for each patient was calculated by the formula: risk score = (1.14515Expr PPIC-AS1) + (−1.94235Expr MME-AS1) + (−0.54517Expr AC012676.3) + (1.246856Expr MFF-DT) + (0.83774Expr OTUD6B-AS1) + (−0.49163Expr AL109936.9) + (−0.87639Expr AL807757.2) + (−1.34052Expr AL118556.1) + (−2.04930Expr AL137847.1)."
38353835
sparser
"OTUD6B-AS1 expression is downregulated in HCT116 cells and CRC tissues."
33574932
sparser
"In TNBC cells resistant to paclitaxel, lncRNA OTUD6B-AS1 is identified to promote autophagy and DNA damage through the regulation of the miR-26a-5p/MTDH axis."
37958880
sparser
"In summary, we first investigated the expression pattern of OTUD6B-AS1 in ccRCC tissue and cell lines."
30670025
sparser
"OTUD6B-AS1 may be an indicator of poor prognosis in patients with ccRCC."
30670025
sparser
"The binding site between OTUD6B-AS1 and miR-3171 was predicted using Starbase 2.0 ( xref )."
33574932
sparser
"However, one of the yet undetermined mechanisms not addressed in the current study is whether OTUD6B-AS1 can function as a competitive endogenous RNA (ceRNA) to regulate microRNAs (miRNAs) during ccRCC development."
30670025
sparser
"miR-183-5p and miR-21 Were Direct Targets of OTUD6B-AS1."
32256450
sparser
"Next, we screened potential targets of OTUD6B-AS1 using several online programs, OncomiR, StarBase 3.0 and LncBase Predicted v."
32256450
sparser
"Since ceRNAs represent a novel aspect of regulation, especially during tumorigenesis [ xref ], ongoing work is strongly needed to determine whether OTUD6B-AS1 could be a candidate ceRNA during ccRCC progression."
30670025
sparser
"However, to the best of our knowledge, the role of OTUD6B-AS1 in CRC has not yet been determined."
33574932
sparser
"Using Starbase, it was predicted that OTUD6B-AS1 can bind to miR-3171."
33574932
sparser
"Overexpression of OTUD6B-AS1 inhibits the proliferation, invasion and migration of HCT116 cells."
33574932
sparser
"Furthermore, the overexpression of OTUD6B-AS1 significantly decreased proliferation and tumorigenesis while partly inhibiting cell migration and invasion in ccRCC cells in vitro."
30670025
sparser
"More importantly, no study has revealed the molecular mechanism and downstream targets of OTUD6B-AS1 until now."
30670025
sparser
"Here, we demonstrated that the antioncogenic effect of OTUD6B-AS1 is partly mediated through the inhibition of the activity of the Wnt/β-catenin pathway and the EMT-related pathway."
30670025
sparser
"Therefore, it could be concluded that the overexpression of OTUD6B-AS1 suppressed the proliferation, invasion and migration of CRC cells."
33574932
sparser
"Additional studies are needed to determine whether OTUD6B-AS1 modulates other targets in ccRCC; however, our findings nonetheless provide novel insight into ccRCC pathogenesis as well as a basis for the improvement of individualized treatment for ccRCC patients."
30670025
sparser
"Moreover, the MTF1 expression level could be elevated by As2O3, participating in the regulation of lncRNA OTUD6B-AS1 in responding to oxidative stress [ xref ]."
37380924
sparser
"In order to verify the predictions of the online programs, luciferase reporter plasmids, psiCHECK-2-WT, containing wild type (WT) OTUD6B-AS1 were created using the psiCHECK-2 vector."
32256450
sparser
"To determine whether OTUD6B-AS1 can exert its effects on proliferation, invasion and migration of HCT116 cells by targeting miR-3171, a series of functional experiments was performed."
33574932
sparser
"Furthermore, as shown in xref , OTUD6B-AS1 overexpression notably suppressed the invasion and migration of HCT116 cells compared with those of cells in the empty vector (Oe-NC) group."
33574932
sparser
"In a series of assays, it was observed that the additional treatment with miR-3171 mimics following OTUD6B-AS1 overexpression in HCT116 cells promoted the proliferation, invasion and migration of HCT116 cells, suggesting that OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells via downregulation of miR-3171 expression."
33574932
sparser
"In conclusion, to the best of our knowledge, the present study was the first to investigate the role of OTUD6B-AS1 in CRC cells, and to reveal that lncRNA OTUD6B-AS1 overexpression inhibited the proliferation, invasion and migration of HCT116 cells, at least partially, by targeting miR-3171."
33574932
sparser
"Therefore, OTUD6B-AS1 may serve as a potential novel biomarker and target for the diagnosis and treatment of CRC."
33574932
sparser
"Additionally, the use of only one TCGA dataset to investigate OTUD6B-AS1 expression is another limitation of the present study, and future studies should improve upon this and the other limitations."
33574932
sparser
"The Overexpression miR-183-5p and miR-21 Partly Compromised the Inhibitory Effects of OTUD6B-AS1 on Migration and Invasion."
32256450
sparser
"We speculated that OTUD6B-AS1 regulates the migration and invasion of thyroid carcinoma by down-regulating miR-183-5p and miR-21."
32256450
sparser
"To verify this speculation, we investigated whether the up-regulation of miR-183-5p and miR-21 can compromise the inhibitory effects of OTUD6B-AS1."
32256450
sparser
"Additionally, compared with OTUD6B-AS1 group, miR-183-5p and miR-21 expression were significantly increased after co-transfected OTUD6B-AS1, miR-183-5p, and (or) miR-21 mimic into SW579 and TPC-1 cells ( xref )."
32256450
sparser
"Additionally, miR-21 and miR-183-5p overexpression had stacked effect to reverse the effect of OTUD6B-AS1 on the viability, migration, and invasion abilities ( xref , xref )."
32256450
sparser
"OTUD6B-AS1 was first reported in systemic sclerosis ( xref )."
32256450
sparser
"In the field of cancer research, Wang and colleague reported OTUD6B-AS1 was downregulated in ccRCC tissue samples, and that patients with low OTUD6B-AS1 expression had shorter overall survival than patients with high OTUD6B-AS1 expression, which showed that the different expression levels of OTUD6B-AS1 indirectly correlated with patient survival ( xref )."
32256450
sparser
"Previous studies have reported that elevated expression of MRCCAT1, ATB, and SNHG14 in KIRC were correlated with poor prognosis, and this is also the case for low expression of OTUD6B-AS1 and ADAMTS9-AS2."
32974157
sparser
"On the other hand, Wang et al. demonstrated that the antioncogenic effect of OTUD6B-AS1 is partly mediated through the inhibition of the activity of the Wnt/β-catenin pathway and the EMT-related pathway ( xref )."
32974157
sparser
"Gang Wang et al. demonstrated that lncRNA OTUD6B-AS1 not only indicates poor prognosis but also inhibits ccRCC proliferation via the Wnt/β-catenin signaling pathway [ xref ]."
35891325
sparser
"Inc-PIGM-1, PTOV-AS1, BAIAP1, PTPRJ-AS1, which have critical roles in inflammation, GNG12-AS1 and OTUD6B-AS1, that responsible for proliferation, lncZBTB25-2, lnc-RBMX-1, lNC-HAD2-2, lncMRPS30-8 and l[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38042283
sparser
"We reported downregulation of lncRNA OTUD6B-AS1 in thyroid cancer."
32256450
sparser
"The different expression level of OTUD6B-AS1 correlated with tumor size, clinical stage and lymphatic metastasis of thyroid carcinomas."
32256450
sparser
"Here, we aimed to evaluate the endogenous cellular mechanisms involved in As 2 O 3 -mediated oxidative damage through regulation of lncRNA ovarian tumor domain containing 6B antisense RNA1 (lncRNA OTUD6B-AS1)."
32952848
sparser
"The result suggested that lower OTUD6B-AS1 expression was closely related to the growth and metastasis of thyroid cancer."
32256450
sparser
"Collectively, our results reveal that As 2 O 3 provokes oxidative stress by upregulating lncRNA OTUD6B-AS1, which downregulates mitochondrial NADP + -dependent isocitrate dehydrogenase 2 (IDH2) by stabilizing its negative regulator, miR-6734-5p."
32952848
sparser
"Consistent results in thyroid cancer and ccRCC indicate down-regulation of OTUD6B-AS1 as important for cancer development."
32256450
sparser
"As 2 O 3 Increases lncRNA OTUD6B-AS1 Expression."
32952848
sparser
"First, we investigated the effect of As 2 O 3 on lncRNA OTUD6B-AS1 expression."
32952848
sparser
"Under different physiological and pathological conditions, OTUD6B-AS1 is implicated in different cellular biological functions."
32256450
sparser
"The results of qRT-PCR analyses showed that lncRNA OTUD6B-AS1 levels in T24 bladder cancer cells were significantly and dose-dependently increased in response to As 2 O 3 ; lncRNA OTUD6B-AS1 levels were increased by more than 8.65-fold by As 2 O 3 treatment at 20  μ mol/L for 6 h, relative to untreated T24 cells ( P < 0.01) ( xref )."
32952848
sparser
"In systemic sclerosis, OTUD6B-AS1 functions as a regulator of apoptosis ( xref )."
32256450
sparser
"OTUD6B-AS1 regulates proliferation and apoptosis via cyclin D1 expression in a sense gene independent manner ( xref )."
32256450
sparser
"In tumors, OTUD6B-AS1 regulates proliferation, migration and invasion."
32256450
sparser
"In the current study, we reported that OTUD6B-AS1 overexpression inhibited cells viability, migration, and invasion, similar with the effect of OTUD6B-AS1 in ccRCC."
32256450
sparser
"One of the important findings of this study is our identification of two downstream targets of OTUD6B-AS1, miR-183-5p, and miR-21."
32256450
sparser
"We showed that miR-21 is a direct target of OTUD6B-AS1."
32256450
sparser
"In this research, we explored the role and mechanism of lncRNA ovarian tumor domain containing 6B antisense RNA1 (OTUD6B-AS1) in CRC."
33686892
sparser
"Here, we detected OTUD6B-AS1 expression in CRC tissues and cells by RT-qPCR."
33686892
sparser
"This result found that miR-21 overexpression promoted the viability, migration, and invasion, reversed OTUD6B-AS1 effect in thyroid carcinomas."
32256450
sparser
"Functionally, overexpression of OTUD6B-AS1 inhibited cell proliferation, migration, invasion and EMT, and promoted cell apoptosis."
33686892
sparser
"OTUD6B-AS1 and miR-21 constitute a regulatory axis within thyroid carcinomas."
32256450
sparser
"Overall, OTUD6B-AS1 inhibits cellular development in CRC by sponging miR-21-5p and upregulating PNRC2, providing a novel insight into the exploration on CRC treatment."
33686892
sparser
"miR-183-5p is another direct target of OTUD6B-AS1."
32256450