IndraLab

Statements

UCHL5 activates autophagy. 5 / 6
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"To address whether downregulation of proteasome-associated DUBs ubh-4, the uchl5 homolog, and usp-14 induces a tissue-specific or systemic effect on autophagy in C. elegans, we downregulated ubh-4 and usp-14 by RNAi-feeding and analyzed autophagy in intestinal cells, hypodermal seam cells and pharynx (Fig. 4A)."
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"We show that downregulation of Uchl5 by siRNA reduces autophagy by partially blocking the fusion of autophagosomes with the lysosomes in HeLa cells, which is similar to a previously reported role of the proteasome-associated DUB Usp14 on autophagy."
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"Our data reveal that Uchl5 and Usp14 siRNA treatments or pharmacological inhibition reduce autophagy by blocking autophagosome–lysosome fusion in GFP-LC3-RFP-LC3ΔG HeLa cells."
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"It is unlikely that the impact of Usp14 on autophagy would override that of Uchl5, as our siRNA results show that both Usp14 and Uchl5 knockdown reduces autophagy."
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"To investigate whether Uchl5 or Usp14 knockdown causes a block in autophagy or potentially affect the number of lysosomes, we first performed immunofluorescence analysis against lysosome-associated membrane protein 2 (LAMP2)."
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