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USP10 binds CAPRIN1. 27 / 27
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"However, it is important to note that the interactions between G3BP1 and USP10 or Caprin1 do not fully dictate SG disassembly or assembly, as two different laboratories recently demonstrated that the unstressed G3BP1 interactome significantly overlaps the interactome of G3BP1 during stress [XREF_BIBR, XREF_BIBR]."
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"Subsequent work demonstrated that in addition to homo- and hetero-dimerization, G3BP1 and 2 interact with USP10 and Caprin1 [ xref , xref , xref , xref ]."
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"Impact of G3BP1 F33W mutation on USP10 and Caprin1 peptide binding."
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"G3BP mediated SG assembly is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region that allows interactions with 40S subunits."
27022092
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"G3BP1-F33W is SG competent despite its inability to bind Caprin1 or USP10, suggesting that G3BP is only regulated by Caprin1/USP10."
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"As a result, this disjunction between USP10 and Caprin1 binding may well reflect differences in the motif peptides, since the USP10 FGDF motif contains two phenylalanines, whereas the Caprin1 FIQDSMLD motif contains only one."
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"SG formation is rescued in these cells by expression of either wild-type G3BP, or G3BP containing a point mutation that renders it unable to bind USP10 or Caprin1."
30082464
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"Nancy presented preliminary data indicating differential regulation of SG nucleation by these G3BP1 binding partners, whereby Caprin1 and USP10 bind the G3BP-NTF2-like domain in a competitive manner, with opposite results; G3BP1:Caprin1 complexes promote SG formation, whereas G3BP1:USP10 complexes inhibit SG formation."
25256393
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"In addition, the acetylation-mimicking mutation K376Q impaired the RNA-dependent interaction of G3BP1 with PABP1, but its RNA-independent interactions with Caprin-1 and USP10 were little affected."
31481451
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"Recently, Kedersha and colleagues reported that SG assembly is finely regulated by the binding of G3BPs to Caprin1 or USP10 [ xref ]."
27367717
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"Rescue experiments using a G3BP1 mutant that lacks the ability to bind Caprin1 or USP10 rescues SGs assembly, whereas other phosphomimetic mutants of G3BP1 (G3BP1-S149E) fail to do that [ xref ]."
30595162
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"In contrast, G3BP1 mutants (G3BP1Δ1–11, G3BP1Δ1–50, and site-specific mutant G3BP1-F33W) unable to bind USP10 ( xref ) or Caprin1 ( xref ) fail to precipitate either protein ( xref , lanes 2, 7, and 8)."
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"Stress granule condensation (SGC) of translationally arrested mRNAs requires G3BP, and G3BP-mediated SGC is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region for SGC and for interactions with 40S ribosomal subunits."
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"A mutant of G3BP incapable of binding to either Caprin1 or USP10 is fully SG competent, whereas overexpression of Caprin1 in the ΔΔG1/G2 cells does not restore SGs ( xref ; xref )."
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"Additionally, several groups have shown that mutating F33 to a tryptophan ablates Caprin1 and USP10 binding (8, 26, 31, 32) ."
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"The WT FGDF peptide displaces both Caprin1 and USP10 from immobilized G3BP1, but mutant F10A peptide does not, suggesting that Caprin1 and USP10 bind within the same region of G3BP ( xref )."
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"Coimmunoprecipitation of 40S proteins is not inhibited by G3BP1 mutations (F33W, Delta1-11, and Delta1-50) that abrogate USP10 and Caprin1 binding (XREF_FIG, lanes 2, 7, and 8) or by mutation of S149 (lanes 4 and 5)."
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"It is still possible that S149 phosphorylation influences the mutually exclusive interaction of USP10 and Caprin1 in the context of recovery and disassembly of SGs, whereby the interaction of USP10 is favored and acts as a “decondensase” of SGs ( xref )."
31171631
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"G3BP-mediated SG assembly is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region that allows interactions with 40S subunits."
27022092
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"Tryptophan mutations in G3BP1 tune the stability of a cellular signaling hub by weakening transient interactions with Caprin1 and USP10."
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"Previous crystal structures have demonstrated the importance of hydrophobic interactions and -stacking in the G3BP1 groove for USP10 and Caprin1 peptide binding (17, 26, 30) ."
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"In addition to the known levels of control by post-translational modifications, the ability of G3BP1 to nucleate SGs has been linked to its interaction with either Caprin1 or USP10 competing for the NTF2 motif on G3BP1 in a mutually exclusive manner, where USP10 plays an inhibitory anti-aggregation role [ xref ]."
31905230
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"Stress granule condensation (SGC) of translationally arrested mRNAs requires G3BP, and G3BP mediated SGC is inhibited by serine 149 phosphorylation, regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region for SGC and for interactions with 40S ribosomal subunits."
27022092
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"We propose that G3BP mediates the condensation of SGs by shifting between two different states that are controlled by the phosphorylation of S149 and by binding to Caprin1 or USP10."
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"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10 (Ward et al., xref )."
31681621
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"In addition, a quantitative mass spectrometry study revealed that DENV-2 RNA interacts with the SG components G3BP-1/2, Caprin1, and USP10 (Ward et al., 2011)."
31681621
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"Our data suggest that G3BP is necessary to mediate SG assembly via p-eIF2α and eIF4A. To examine the roles of its interacting partners Caprin1 and USP10, we assessed their SG nucleating abilities alone or in combination."
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