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IL6 phosphorylates STAT3 on tyrosine. 70 / 70
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"IL-6 stimulation led to tyrosine phosphorylation of STAT3 (data not shown)."
| DOI
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"Because IL-6 has been found to increase the levels of serine pSTAT3 and induce the phosphorylation of STAT3 on tyrosine residues XREF_BIBR, we incubated CLL cells with 30 mg/ml rhIL-6 for 20 minutes and analyzed their miR-155 expression levels at different times following incubation using RT-PCR and relative qRT-PCR."
23750211
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"As shown in XREF_FIG d, IL-6 did not induce tyrosine phosphorylation of STAT-3 in these T cells."
12370259
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"Physiologically, STAT3 is phosphorylated first by the IL-6 receptor; in its tyrosine phosphorylated form, STAT3 dimerizes and migrates to the nucleus, where it binds to its DNA response elements."
12710894
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"Interleukin-6 (IL-6) induces tyrosine phosphorylation and activation of the latent transcription factor Stat3 in HepG2 cells."
8626374
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"We also confirmed that 8226 cells lack constitutively activated STAT3, whereas IL-6 stimulated the tyrosine phosphorylation of STAT3 and the activation of MAPK, as previously described."
21164517
sparser
"Mechanistically, FXT suppressed the IL-6-induced phosphorylation of tyrosine residue (Tyr705) of signal transducer and activator of transcription 3 (STAT3) probably by binding to STAT3."
31080186
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"Whereas IL-6 stimulated tyrosine phosphorylation of STAT3 in a time- and dose dependent way, DXM and BTM had no effect on STAT3 phosphorylation."
20693312
sparser
"The IL-6 (10 ng/mL)-induced phosphorylation of JAK2 and STAT3 tyrosine residues was significantly inhibited after the cells were treated for 30 min."
33505935
rlimsp
"Representative western blot analysis showing that AG490 (50 μM) and S3I-201 (300 μM) suppressed the tyrosine phosphorylation of STAT3 by IL-6."
24520293
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"We next sought to determine whether the effects of Wit were limited to EGF and IL-6 stimulation of STAT3 and/or STAT5 tyrosine phosphorylation."
24983364
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"To inhibit STAT3 activity, we utilized C188-9, a specific STAT3 inhibitor.28, 33, 34 As expected, C188-9 treatment reduced the tyrosine phosphorylated levels of STAT3 (Y705) in both two cell lines whereas IL-6, a known STAT3 activator, stimulated the tyrosine phosphorylation of STAT3."
| PMC
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"IL-6 activates kinases belonging to the JAK family, namely JAK1, JAK2 and Tyk2 [26,27], leading to the tyrosine phosphorylation of STAT1 and STAT3 proteins."
10606754
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"STAT3 is rapidly tyrosine phosphorylated in response to IL-6, ciliary neurotrophic factor, oncostatin M, leukemia inhibitory factor, IL-11, granulocyte colony stimulation factor and epidermal growth factor."
8918689
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"EBL 's from patients carrying the STAT3 " A " risk allele exhibited increased basal and IL-6 stimulated STAT3 tyrosine phosphorylation, increased transcription of STAT3 and SOCS3 after IL-6 stimulation, and increased membrane localization of the IL-6 receptor, GP130, and JAK2."
22197944
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"IL-6 stimulation induces tyrosine phosphorylation and recruitment of transcriptional factor signal transducer and activator of transcription 3 (STAT3), which dimerizes and is translocated to the nucleus to initiate the transcription of specific genes (5)."
35126375
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"However, the knockdown of IL-6 did not prevent the induction of STAT3 tyrosine phosphorylation."
23114963
sparser
"Stimulation of the BCR significantly increases the constitutive activation of NF-κB, and NF-κB induces the production of IL-6, which successively phosphorylates STAT3 on tyrosine residues in an autocrine or paracrine manner ( xref )."
28722170
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"Cell signaling studies showed that murine OSM, LIF, IL-6, and CT-1 stimulated the tyrosine phosphorylation of STAT-3, suggesting STAT-3 activation is not sufficient for eotaxin induction in NIH 3T3 cells."
12496442
rlimsp
"Tyrosine phosphorylation of STAT3 stimulated by IL-6 alone was not different from that by IL-6 plus glucocorticoid."
9795374
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"The time course of STAT3 tyrosine phosphorylation by IL-6 plus glucocorticoid was not different from that by IL-6 alone."
9795374
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"In M1/WT4 cells IL-6 causes strong tyrosine phosphorylation of STAT3, whereas the response to CSF-1 is weaker."
10427504
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"In this study, we show that IL-6 stimulates a transient increase of tyrosine phosphorylation of STAT3 in a dose dependent fashion."
15894558
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"A major driver of STAT3 activation in malignancy is the IL-6, which signals through a complex of IL-6 receptor and gp130 to activate JAKs and induce tyrosine phosphorylation of STAT3 [XREF_BIBR]."
29254202
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"In macrophages lacking Socs3, however, continuous IL-6 signaling induced uniformly high levels of STAT3 tyrosine phosphorylation, and early IL-6-inducible genes were inappropriately expressed at intermediate time points."
16473883
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"IL-6 induces tyrosine phosphorylation of STAT3 through activation of JAK kinases, but HB-EGF also has the potential to promote STAT3 phosphorylation by binding to and activating the EGF receptor."
22351606
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"It could be demonstrated by using either antibody that IL-6 induced a significant STAT3 tyrosine phosphorylation (lanes 2) and pretreatment of hepatocytes with 100 mM ethanol for 30 min abolished this[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10486586
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"In the case of IL-6 signal transduction, IL-6 induces tyrosine phosphorylation of STAT3, STAT1 and STAT5."
11337018
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"A major driver of STAT3 activation is the cytokine interleukin-6 (IL-6), which signals through a heterodimeric IL-6 receptor (IL-6Ralpha and IL-6Rbeta) to activate JAKs and induce STAT3 tyrosine phosphorylation."
28529032
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"NF-κB–transcribed IL-6 enhances the phosphorylation of STAT3 on tyrosine residues."
28722170
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"Addition of exogenous IL-6 to STAT2 -/- cells induced STAT3 tyrosine phosphorylation (XREF_FIG) thus suggesting that activation of STAT3 is not directly STAT2 dependent, but is mediated by IL-6."
20233899
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"The ability of IL-6 to induce tyrosine phosphorylation of STAT3 was abolished in the LNCaP-IL-6+ subline."
12547723
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"In particular, IL-6 treatment during T-cell activation results in the tyrosine phosphorylation of STAT3, which induces the expression of IL17 [XREF_BIBR]."
26514620
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"Tyrosine phosphorylation of STAT3 stimulated by IL-6 alone was not different from that by IL-6 plus glucocorticoid."
9795374
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"In CLL cells, extracellular IL6 or BCR signaling induces tyrosine phosphorylation of STAT3 [XREF_BIBR, XREF_BIBR], leading to upregulated of anti-apoptosis genes and a survival advantage."
| PMC
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"IL-6 and sIL-6R and OSM treatment induced STAT-3 tyrosine phosphorylation, but had little effect on PIAS3 expression (XREF_FIG and data not shown)."
18676737
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"Our studies found that pretreatment with FD resulted in blockade of activation of fibroblast and GMC manifested by fibronectin (FN) and alpha-smooth muscle actin (alpha-SMA) protein expression and decline of STAT3 tyrosine phosphorylation induced by IL-6 or high glucose."
26033204
rlimsp
"The time course of STAT3 tyrosine phosphorylation by IL-6 plus glucocorticoid was not different from that by IL-6 alone."
9795374
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"IL-6 increased the levels of tyrosine phosphorylation of JAK2 and STAT3 in A549 cells, which was reversed by HMA treatment."
31035048
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"Tyrosine phosphorylation of STAT3 induced by IL-6 has also been linked to effector lymphocyte and granulocyte activation in IBD."
23429443
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"As a set of representative results are shown in Fig. 3a, IL-6 significantly activated tyrosine (Tyr 705) phosphorylation of STAT3 in parental Du-145 cells and Neo clones, and slightly increased tyrosi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11163768
rlimsp
"DCE and CS were also able to down modulate IL-6 dependent-tyrosine phosphorylation of STAT3 and its DNA binding activity in DLD-1 cell line (Figure S1)."
21625597
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"In mouse livers and bone marrow derived macrophages, both interferon-gamma (IFNgamma) and interleukin-6 (IL-6) rapidly induced the tyrosine phosphorylation of signal transducer and activator of transcription-1 (STAT1) and STAT3."
16473883
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"The Asp170 residue in the helix alpha1 of the coiled-coil domain of STAT3 interacts with other transcription factors [XREF_BIBR], and tyrosine phosphorylation of STAT3 by IL-6 is required for its receptor binding, dimerization, nuclear translocation, and DNA binding [XREF_BIBR]."
31952344
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"The JAKs IL-6 receptor associated tyrosine kinase phosphorylates STAT3, promoting its translocation to the nucleus and regulating the acute phase response and inflammation."
23276528
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"While this suggests a number of therapeutic strategies, including blocking antibodies to IL-6 or the IL-6 receptor, there has been great interest in using small molecule inhibitors of the Jak family kinases that mediate STAT3 tyrosine phosphorylation triggered by IL-6."
23531638
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"In agreement with this model, despite high levels of STAT3 Tyr phosphorylation and STAT3 chromatin binding induced by IL-6 treatment, we detected very few genes induced by IL-6 in Th-1 cells, which express high levels of CDK8."
33357429
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"In particular, we found that CD28-activated RelA/NF-kappaB induces the expression of IL-6 that, in a positive feedback loop, mediates the activation and nuclear translocation of tyrosine phosphorylated STAT3 (pSTAT3)."
31068940
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"Tyrosine phosphorylation of STAT3 was induced by IL-6, OSM and LIF."
10922477
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"Immunoprecipitation experiments showed that IL-6 increased tyrosine phosphorylation of Stat1 and Stat3 in PC12 cells, whereas NE caused a sustained increase in tyrosine phosphorylation of Stat1."
10779380
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"In the parental M1 cells, IL-6 caused sustained tyrosine phosphorylation and DNA binding of endogenous STAT3, which reached its maximal level at 10 min (tyrosine phosphorylation) or 15 min (DNA bindin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11337018
trips
"In CD45+ U266 cells, IL-6 increased tyrosine phosphorylation of gp130 and STAT3 and stimulated the level of Mcl-1 protein expression."
14979481
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"Jak1 kinase activity, interleukin 6 induced gene activation, Stat3 tyrosine phosphorylation, and DNA binding were inhibited, as was activation of Jak1 and Stat1 by interferon gamma."
8790359
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"IL6, but not LIF or OSM inhibited proliferation, induced NE differentiation and tyrosine phosphorylation of STAT3 in LNCaP cells."
15313471
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"The IL-6 and Janus-activated kinase can trigger tyrosine phosphorylation of both STAT1 and STAT3 through homo- or hetero-dimerization of the signal transduction subunit gp130 [XREF_BIBR]."
27769057
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"Taken together, these data suggest that stimulation of the BCR induces the production of high IL-6 levels and that IL-6 induces the phosphorylation of STAT3 on tyrosine residues."
28722170
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"Early studies indicated that tyrosine phosphorylation of STAT3 stimulated by interleukin 6 could cause its translocation into nucleus where STAT3 was activated, bound to the enhancer elements of target genes, and formed nuclear bodies."
35628304
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"Collectively, JAB/SOCS-1/SSI-1 is a potent inhibitor of IL6 signaling, including IL6 induced tyrosine phosphorylation of gp 130 and STAT3."
9918119
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"Targeting IL-6 and IL-6 receptor using monoclonal antibodies Siltuximab and Tocilizumab in a K-ras mutant mouse model for lung cancer suppressed cancer progression via inhibition of STAT3 tyrosine phosphorylation (86,87) and polarized macrophages towards an anti-tumor phenotype (87)."
34012800
rlimsp
"In support of this hypothesis, we observed that a short treatment of cells with Ang II transiently inhibited the IL-6-induced Stat3 tyrosine phosphorylation."
9879441
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"To examine whether IL-6 has an autocrine effect on Nrf2/MnSOD, MDA-MB-231 (IV2-3) cells were exposed to IL-6 that activated Tyrosine phosphorylation (Y705) of STAT3 and also enriched Nrf2 and MnSOD in a time-dependent manner (Fig. 4A)."
35017469
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"It is reported that IL6 can also induce signal transduction as well as phosphorylation of transcriptional activator 3 (STAT3) and protein tyrosine kinase 2 (JAK2) by activating the IL6/JAK2/STAT3 signal pathway."
33424592
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"We reported recently that angiotensin II (AII) and phorbol 12-myristate 13-acetate (PMA) transiently inhibit interleukin 6 (IL-6)-stimulated tyrosine phosphorylation of signal transducers and activators of transcription 3 (Stat3) and subsequent formation of sis inducing factor-A (SIF-A)."
8798409
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"In CD45+ U266 cells, IL-6 increased tyrosine phosphorylation of gp130 and STAT3 and stimulated the level of Mcl-1 protein expression."
14979481
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"IL-6 increased phosphorylation of STAT3 (at Tyr (705)) and ERK1/2 (at Tyr (204)) within 5 min that peaked at 15-30 min and returned to basal levels at 2 h. Phosphorylation of STAT3 was blocked by genistein, a protein tyrosine kinase inhibitor, and AG490, a JAK2 inhibitor, but not PD98059, an ERK1/2 kinase inhibitor."
12595539
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"Among the STAT family members, IL-6 has been shown to induce the tyrosine phosphorylation of STAT1 and STAT3, their nuclear translocation, and their binding to a specific DNA element (Akira et al. 199[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8934572
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"Li et al reported that extracellular PBEF is able to attenuate stress induced macrophage apoptosis via activation of the STAT3 pathway by promoting IL-6 tyrosine phosphorylation of STAT3 (XREF_FIG)."
23787158
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"Based on our findings, we propose a revision to the Jak-STAT activation model, in which IL-6 stimulation induces tyrosine phosphorylation of cytoplasmic STAT3, inducing dimerization and nuclear transl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16285960
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"As expected, we found that 50 ng/mL IL-6 activated STAT3 and induced STAT3 tyrosine phosphorylation on Tyr 705 in A549 and HCC827 cells."
24789104
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"It was found that SS prevented the IL-6 stimulated tyrosine phosphorylation of both gp130 and Stat3, blockading signal transduction and completely abolishing IL-6 stimulated production of haptoglobin."
10598880