IndraLab

Statements

EGF activates RAC1. 253 / 288
| 1 156 96
reach
"In addition, depletion of RhoG significantly inhibits EGF stimulated Rac1 activation in these cells."
22966858
sparser
"A second wave of EGF-induced Rac1 activation was required for EGF-induced cell migration, however, the spatiotemporal regulation of the second wave of EGF-induced Rac1 activation remains largely unclear."
24350867
sparser
"In contrast, combined Cbl and Cbl-b KD dramatically enhanced the EGF-induced Vav2 phosphorylation in response to EGF stimulation ( xref G ) and enhanced the EGF-induced Rac1 and Cdc42 activation ( xref , A and B )."
20940296
reach
"A second wave of EGF induced Rac1 activation was required for EGF induced cell migration, however, the spatiotemporal regulation of the second wave of EGF induced Rac1 activation remains largely unclear."
24350867
sparser
"Tiam1 expression is required for the second wave of EGF-induced Rac1 activation in A431 cells."
24350867
reach
"Both EGF and HGF can induce several Rac1 or Cdc42 specific Guanine nucleotide exchange factors (GEFs) [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]; however, it is still unclear which RacGEF (s) are responsible for MLK3 activation."
27213454
sparser
"Additionally, we showed that Rac1 activation by EGF was prevented by addition of an Akt inhibitor (Figure xref )."
27102434
reach
"We also showed that the extent of EGF induced Rac1 activation in MDCK and CIIA-Flag cells was markedly greater than that in MDCK and control cells (XREF_FIG)."
22042618
reach
"Indeed, this concept is supported by a previous study showing that EGF treatment of MCF-7 and MDA-MB-231 breast cancer cells promotes the release and activation of RhoA, Rac1 and Cdc42 by Ser174 phosp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29307615
reach
"Epithelial growth factor (EGF)-mediated activation of Rho GTPase Rac1 and formation of lamellipodia in TS/A cells were remarkably suppressed by DET treatment."
22342517
reach
"Using GLISA, 300 muM R-naproxen pretreatment of Swiss 3T3 cells for 15 min significantly inhibited EGF mediated Rac1 activation relative to EGF stimulated controls and a 1 h pretreatment reduced activation to basal unstimulated levels (XREF_FIG)."
26558612
reach
"Previously, we reported that epidermal growth factor (EGF) induced two waves of Rac1 activation; namely, at 5min and 12h after stimulation."
24350867
reach
"Analysis of Rac1 biological activity showed that Rac1N17 lentivirus particles infection significantly inhibited epidermal growth factor stimulated Rac1 activity in the PFCs, while Rac1L61 lentivirus particles enhanced the Rac1 activity."
20159679
sparser
"We showed that ZINC69391 was able to impair Rac1 activation by EGF, a described activator of Rac1 signaling pathway [ xref ]."
24066799
reach
"As shown in XREF_FIG (left panel), EGF stimulated Rac1 activity was significantly suppressed (XREF_FIG (right panel) in Rab5C KD cells."
24587345
reach
"Thrombin or EGF resulted in activation of Rac1 in CHRF-288-11 or HeLa cells respectively and W7 inhibited this activation."
22905193
sparser
"EGF and HRG also activate Rac1 in other breast cancer cell lines as well as in immortalized MCF-10A mammary cells ( xref )."
21172654
reach
"Treatment with 100 ng/mL EGF increased RAC1, Ref-1, EGR1, and PTEN expression and markedly inhibited Akt phosphorylation in these cells."
27935858
reach
"This compound was able to block Rac1 interaction with its GEF Tiam1, prevented EGF induced Rac1 activation and inhibited cell proliferation, cell migration and cell cycle progression in highly aggressive breast cancer cell lines."
24066799
reach
"These results suggest that HER2 is essential for EGF induced Rac1 activation in SKBR-3 cells."
30515933
sparser
"Therefore, it may be reasonable to believe that EGF‐induced activation of Rac1 and enhanced the expression of TJ and AJ proteins mediated through PI3K/Akt signalling pathway."
26769343
reach
"Exogenous expression of ILK and/or ELMO2 significantly enhanced EGF induced Rac1 activation over the same period (XREF_FIG and XREF_FIG)."
22160594
reach
"We therefore examined this potential mode of action, and found that EGF induced Rac1 activation was regulated by CysLT1 signaling (unpublished data)."
23139868
sparser
"Exogenous expression of ILK and/or ELMO2 significantly enhanced EGF-induced Rac1 activation over the same period ( xref and xref )."
22160594
reach
"These results therefore demonstrate that BRK colocalizes and alters the subcellular localization of KAP3A and the C-terminus of KAP3A is required for KAP3A localization.BRK has been shown to phosphory[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18077133
reach
"Because knockdown of these GEFs suppressed EGF induced Rac1 activation only partially, we looked for another Rac1 GEF downstream of the EGF receptor and found that Asef, a Rac1-Cdc42 GEF bound to the tumor suppressor APC, also contributed to EGF induced Rac1 activation."
18653540
sparser
"Rapid Activation of RhoG and Rac1 by EGF Is Not Interdependent."
20237158
sparser
"Therefore, it may be reasonable to speculate that EGF-induced ERK and Rac1 activation and cell migration require the PH domain of GEP100 and are mediated through the GEP100/Arf6 pathways."
22701712
reach
"Expression of a carboxy-terminal fragment of Vav2 decreased the elevation of Rac1 activity induced by epidermal growth factor, consistent with Vav2 mediating activation of Rac1 downstream from growth factor receptors."
10982832
sparser
"Previously, we set up controls for this assay, such as a known model of Cdc42 and Rac1 activation by EGF in HeLa cells ( xref ( xref ), and GDP-loaded, and GTPγS-loaded HeLa protein extracts ( xref )."
25862245
reach
"Epidermal growth factor stimulates Rac1 activation through Src and PI3K to promote colonic epithelial cell migration XREF_BIBR and induces the ERK and Rac1 signaling pathway to promote cell migration in human hepatoma HepG2 cells XREF_BIBR."
26078726
reach
"3.4 EGF induced Rac1 activation is abolished by RV treatment."
21732534
reach
"Furthermore, specific downregulation of PI3K and Akt signaling in MDA-MB-231 cells did not alter EGF induced activation of Rac1."
23554696
reach
"However, EGF stimulation causes Rac-1 mediated internalization of E-cadherin via macropinocytosis."
19055694
sparser
"In addition, emodin markedly suppressed EGF-induced activation of Cdc42 and Rac1 and the corresponding cytoskeleton changes."
15928809
sparser
"We showed that inhibition of membrane-localized ERK activity potentiates EGF-induced Rac1 activation and leads to enhanced membrane protrusion dynamics."
32452765
reach
"The EGF induced activation of Rac1 in cells exogenously expressing ILK and ELMO2 suggests Rac1 may mediate ILK and ELMO2 modulation of cell polarization."
22160594
sparser
"FIP-fve inhibited EGF-induced activation of Rac1."
24274472
sparser
"Rac1 dissociation from RhoGDI and subsequent Rac1 activation induced by the growth factors EGF and PDGF required phosphorylation of S101 and S174 by Pak1."
16472651
reach
"Conversely, 10 ng/ml EGF (a known stimulator of both Rac1 and O (2) (-) release) increased ENaC NP (o) values in both type 1 and 2 cells."
20097736
sparser
"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively ( xref , xref )."
30541856
reach
"In contrast, combined Cbl and Cbl-b KD dramatically enhanced the EGF induced Vav2 phosphorylation in response to EGF stimulation (XREF_FIG G) and enhanced the EGF induced Rac1 and Cdc42 activation (XREF_FIG, A and B)."
20940296
reach
"This result suggested that EGF induced Rac1 activation may occur transiently and/or locally in SKBR-3 cells and, thus, it might be difficult to detect Rac1-GTP from total cell lysates by the pull-down assay."
30515933
sparser
"ZINC69391 Interferes Rac1 Activation by EGF."
24066799
reach
"Rac1 can be activated by EGF and other growth factors that elicit the exchange of GTP for the GDP bound to inactive Rac (Dise et al., 2008)."
18487071
reach
"Because the PI3K and Akt signaling pathway plays a vital role in EGF induced migration by activating Rac1 and Cdc42, that are important for lamellipodia and filopodia formation respectively, and found upregulated in metastatic lesions, we further examined the activation status of Rac1 and Cdc42 in the absence or presence of EGF by G-LISA."
27167206
reach
"MCF-7 cells were stimulated with EGF and IGF-I, which activate Rac1 in a P-Rex1-dependent manner [XREF_BIBR], as well as with 10% FBS."
29983884
reach
"To assess the involvement of Rac1 in the EGF induced invasive phenotype of Hs578T and MDA-MB-231 cells, we first examined if EGF activated Rac1 in these cells."
21281602
sparser
"We previously found that the EGF-induced biphasic activation of Rac1 and the second-wave Rac1 activation require the upregulation of Tiam1, a member of the Rac GEF family. xref We also demonstrated that CysLT1 signaling played an important role in Tiam1 upregulation required for the second EGF-induced wave of Rac1 activation."
24350867
reach
"In order to understand role for Rac1 in IMC-C225 mediated inhibition of cell invasion, pull-down assays using the PBD of PAK were performed to measure Rac1 activation in MDA-MB468 cells treated with either EGF or with IMC-C225 for the same time points as studied for RhoA activation."
18829495
reach
"In the present study, it was found that EGF induced Rac1 activation in vivo and in vitro."
26769343
reach
"We used HeLa and Swiss 3T3 cells for these studies because the robust Rac1 activation induced by EGF stimulation in these cells serves as an excellent positive control."
29746086
reach
"Rac1 activation by EGF was inhibited by gefitinib, lapatinib or SB203580 but amplified by PD98059."
23411345
sparser
"S100A7 overexpression affect EGF-induced Rac1 activation and associated signaling."
23535840
sparser
"This result suggested that EGF‐induced Rac1 activation may occur transiently and/or locally in SKBR‐3 cells and, thus, it might be difficult to detect Rac1‐GTP from total cell lysates by the pull‐down assay."
30515933
sparser
"U73122 treatment prevented Rac1 activation by EGF, whereas its inactive analogue did not."
22384062
reach
"EGF stimulated Rac1 activation is rapid and transient."
24587345
sparser
"It was further shown that EGF activates Rac1 through the activation of Tiam1, either via 14-3-3ζ or through 5-lipoxygenase-leukotriene C4 [ xref , xref ]."
30544910
reach
"We examined whether EGF induced changes in fibronectin and phospho-Rac1 and cdc42, which in turn increase cell migration, are mediated by COX-2 induction in HNSCC."
25595899
reach
"We showed that in A431 cells PLEKHG6 together with Vav family GEFs is involved in the rapid activation of RhoG after EGF stimulation (XREF_FIG, D and E), whereas EGF induced Rac1 activity is not influenced by this GEF."
20237158
sparser
"Recently, it has been shown to localize at the leading edge of migrating glioma cells where it promoted membrane ruffling and migration and invasion of glioma cells, as well as the EGF-induced activation of Rac1 ( xref )."
24109588
sparser
"Rac1 can be activated by EGF and other growth factors that elicit the exchange of GTP for the GDP bound to inactive Rac ( Dise et al., 2008 )."
18487071
reach
"Similar to BDNF, our results show that miR-204 inhibits EGF induced activation of Rac1."
23285024
sparser
"In the present study, we found that overexpression of e3B1 in NIH3T3/EGFR cells sensitized EGF-induced activation of Rac1, whereas it had no impact on EGF-induced activation of p21Ras."
16182283
reach
"Epidermal growth factor stimulates Rac1 and p21 activated kinase in vascular smooth muscle cells."
17350025
reach
"In the present study, we demonstrate that MEK activation by EGF increased Rac1 activation, dissociation of intercellular contacts, and migration in both control and polyamine depleted cells, while U0126, a specific inhibitor of MEK1, prevented disruption of junctions as well as EGF induced Rac1 activation."
17183546
sparser
"The level of the active form of Rac1(Rac1-GTP) decreased by the knock-down of H-Ras using siRNA targeting H-Ras, demonstrating that activation of Rac1 by EGF was dependent on H-Ras in Hs578T and MDA-M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21281602
reach
"These data suggest that EGF acting through MEK1 and ERK to activate Rac1 regulates cell-cell contacts."
17183546
reach
"EGF stimulation activated Rac1 and Rho (but not cdc42) in FG cells expressing vector control, F10-15, or F1-9 mutations (XREF_FIG)."
19208836
reach
"This is consistent with our results, since EGF induced activity of Rho and Rac1 in FG cells expressing the CAS F1-9 or F10-15 mutations."
19208836
sparser
"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively (28, 32)."
30541856
sparser
"This Rac1 activation by CIIA*-Myc in the cells was abrogated by RNAi-mediated depletion of EPS8, suggesting that a positive effect of CIIA on EGF-induced Rac1 activation requires EPS8."
22042618
sparser
"Further investigation suggested involvement of the Eps8/Abi1/Sos1 tricomplex; we found up‐regulated expression of the Rac1 guanine nucleotide exchange factor, Sos1, in PDAC tissue and cell lines (supplementary material, Figure xref A, B), and Sos1 siRNA knockdown suppressed EGF‐induced Rac1 activation (supplementary material, Figure xref C)."
28608476
sparser
"Depletion of ARF1 significantly inhibited EGF-dependent activation of Rac1 ( Fig. 2 B)."
23707487
reach
"Pretreatment of VSMC by EGF receptor specific tyrosine kinase inhibitor AG1478 and non specific tyrosine kinase inhibitor genistein inhibited EGF induced activation of Rac1, PAK and JNK, whereas tyrosine kinase inhibitors specific for Src (PP1) and specific for platelet derived growth factor (AG1296) had no effect."
17350025
reach
"In the present study, we found that overexpression of e3B1 in NIH3T3 and EGFR cells sensitized EGF induced activation of Rac1, whereas it had no impact on EGF induced activation of p21Ras."
16182283
sparser
"These results suggest that HER2 is essential for EGF‐induced Rac1 activation in SKBR‐3 cells."
30515933
reach
"Thus, the FRET analysis is consistent with the biochemical data, indicating that Rac1 and to a lesser extent Cdc42 are activated by EGF and that both GTPases are sensitive to moderate cytosolic acidification."
20156964
reach
"However, our results show that EGF treatment increases the activation of RhoA and Cdc42 as well as Rac1, and this event is suppressed by PPM1B in human breast cancer cell lines."
29307615
reach
"R-naproxen and R-ketorolac inhibit serum and EGF-stimulated Rac1 and Cdc42 activation and downstream signaling through a proposed allosteric mechanism [48,95]."
30261690
reach
"EGF stimulation of wild type Rac1 or Rac1 (R163A) in HeLa cells led to the activation of Rac1 (XREF_FIG)."
22905193
reach
"Because of the fast activation of RhoG and Rac1 by EGF treatment (30 s) and to separate these events from later (potentially secondary) activation of these GTPases, we refer from here on to their activation at 30 s as the " rapid activation. ""
20237158
reach
"EGF activates RhoA and Rac1 through activation of the EGF pathway [XREF_BIBR, XREF_BIBR]."
23721065
reach
"Consequently, we examined whether ILK and ELMO2 species can modulate EGF induced Rac1 activation in epidermal keratinocytes."
22160594
reach
"However, upon RNAi depletion of Ect2 from A549 cells we could not find any significant changes in Rac1 activation induced by EGF (C.L.H. and M.G.K., unpublished studies)."
22384062
reach
"Tiam1 expression is required for the second wave of EGF induced Rac1 activation in A431 cells."
24350867
sparser
"PTX also inhibited the activation of Rac1 by EGF (data not shown)."
21172654
sparser
"Consistent with these results, overexpression of WT HA-Cbl diminished whereas that of HA-Cbl-C3AHN enhanced the EGF-induced Rac1 activation ( xref , A and B )."
20940296
reach
"Recently, it has been shown to localize at the leading edge of migrating glioma cells where it promoted membrane ruffling and migration and invasion of glioma cells, as well as the EGF induced activation of Rac1."
24109588
reach
"Asef phosphorylation at Tyr94 plays an essential role in the EGF induced activation of Rac1 and Cdc42 and functions in an Src dependent manner in A431 cells [4]."
24378532
reach
"Activity of RAC1 increases by TGF-beta, but also by EGF, and both act synergistically to get maximum effects."
16331683
sparser
"Next, we examined the actions of CIIA variants on EGF-induced Rac1 activation in 239T cells that had been transfected with vectors for HA-SOS1 and Flag-tagged CIIA variants (CIIA, CIIA-ΔC, and CIIA-ΔN)."
22042618
reach
"An extensive functional analysis using P-Rex1 overexpressing breast cancer cell lines established the requirement of this GEF for Rac1 activation and cell migration induced by EGF, TGFalpha or HRG."
21893191
reach
"We suggest that EGF induced activation of Rac1 causes the activation of mTORC2 via RICTOR."
31995655
sparser
"The suppression of EGF-induced Rac1 activation in siRNA-treated cells was restored by wild-type Asef, but not by the Tyr94Phe mutant of Asef."
18653540
sparser
"Ten minutes after EGF stimulation, the FRET signal became prominent at membrane ruffles at the dorsal cell surface (Figure  xref D,E; Video xref ), suggesting that EGF‐dependent activation of Rac1 occurred transiently and locally in SKBR‐3 cells."
30515933
reach
"Epidermal growth factor (EGF) stimulation substantially increases the activation of Rac1 in control P cells but essentially has no effect in activating Rac1 in cultures with 70% Ppp4 depletion."
18487071
reach
"Moreover, the presence of both LY294002 and PP2 together completely blocked EGF stimulated Rac1 activation (0.8 +/- 0.3 fold-increase; Fig. 3 A and B)."
19116140
reach
"EGF increases Rac1 activity 5- to 10-fold above basal levels with maximal activity evident at 2 min post-stimulation in Swiss 3T3 fibroblasts and HeLa cells."
26558612
reach
"Further investigation suggested involvement of the Eps8/Abi1/Sos1 tricomplex; we found up-regulated expression of the Rac1 guanine nucleotide exchange factor, Sos1, in PDAC tissue and cell lines (supplementary material, Figure XREF_SUPPLEMENTARY A, B), and Sos1 siRNA knockdown suppressed EGF induced Rac1 activation (supplementary material, Figure XREF_SUPPLEMENTARY C)."
28608476
reach
"Importantly, Armus plays a key role in EGF induced scattering and Arf6 dependent disassembly of junctions, by integrating Rac1 activation with Rab7 function to promote E-cadherin degradation.By using [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20116244
reach
"These data demonstrate that ' hyper-stimulation ' of the EGF pathway, which induces Rac1 activation, is sufficient to promote the uptake of a non invasive C. jejuni strain."
21999233
sparser
"Thrombin and EGF respectively resulted in the activation of Rac1 in CHRF-288-11 cells and HeLa cells respectively while W7 inhibited the activation of Rac1 by thrombin and EGF."
22905193
sparser
"Inhibition of ERK activity by U0126 or suppression of Rac1 activity by ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) significantly prevents EGF-induced cell migration, suggesting that EGF-induced ERK and Rac1 activation was responsible for the migration of these cancer cells."
22701712
reach
"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively (28, 32)."
30541856
sparser
"Expression of dominant negative Rac1 in A431 cells abolished Rac1 activation by EGF and prevented binding of the Arp2/3 complex to vinculin induced by EGF ( xref A)."
12473693
reach
"Following treatment, both compound # 1 and # 6 diminished the EGF induced Rac1 activation in a dose dependent manner, with a nearly complete inhibition observed at 50 muM for both compounds."
28410221
sparser
"Assessment of Rac1 activation by EGF using a p21-binding domain (PBD) pull-down assay in Rac-GEF KD cells revealed that only FARP1 depletion reduced Rac1-GTP levels significantly ( xref )."
34731623
reach
"Therefore, it may be reasonable to believe that EGF induced activation of Rac1 and enhanced the expression of TJ and AJ proteins mediated through PI3K and Akt signalling pathway."
26769343
sparser
"Asef phosphorylation at Tyr94 plays an essential role in the EGF-induced activation of Rac1 and Cdc42 and functions in an Src-dependent manner in A431 cells [4] ."
24378532
reach
"Therefore EGF induced activation of Rac1 promotes T-cad relocalization downstream of EGFR phosphorylation."
23411345
sparser
"Furthermore, specific downregulation of PI3K/Akt signaling in MDA-MB-231 cells did not alter EGF-induced activation of Rac1."
23554696
sparser
"However, further Rac1 activation by EGF was absent in CdGAP KD podocytes (Fig.  xref D,E)."
36333327
reach
"RNAi mediated depletion of SOS1 suppressed the EGF induced activation of Rac1 in HeLa cells (XREF_FIG), showing that SOS1 mediates Rac1 activation by this growth factor."
22042618
reach
"The level of the active form of Rac1 (Rac1-GTP) decreased by the knock-down of H-Ras using siRNA targeting H-Ras, demonstrating that activation of Rac1 by EGF was dependent on H-Ras in Hs578T and MDA-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21281602
sparser
"Cell migration is driven by the second wave of EGF-induced Rac1 activation."
30544910
reach
"The affinity reagent PBD interacts only with the activated state of Rac1, which is induced here by epidermal growth factor (EGF) stimulation."
21858900
reach
"The results demonstrated that CHAG treatment efficiently blocked the phosphorylation and activation of EGFR, integrin and VEGFR, inhibited the EGF induced signaling of MAPK and ERK, PI3K and Akt and Rac1 mediated pathways, and diminished the EGF induced expression of proliferation and migration related proteins."
27589842
reach
"As shown in Figure 5F, depletion of Vav1 in the CAPAN2 cell line results in a defect in the basal and EGF stimulated activation of the Rac1 effector molecule Pak1."
15652748
reach
"However, in our study, blocking ERK activity significantly prevents EGF induced Rac1 activation."
22701712
sparser
"Our data provide evidence for EGF-induced Rac1 activation and implicate PAK and JNK as downstream targets of Rac1 in EGF signal transduction in VSMC."
17350025
reach
"Rac1 dissociation from RhoGDI and subsequent Rac1 activation induced by the growth factors EGF and PDGF required phosphorylation of S101 and S174 by Pak1."
16472651
reach
"EGF and HRG also activate Rac1 in other breast cancer cell lines as well as in immortalized MCF-10A mammary cells (XREF_SUPPLEMENTARY)."
21172654
reach
"Similar to what we observed concerning EGF induced regulation of Rac1, the two sublines differed with regard to the dose dependent effect of EGF on activation of Rap1."
16182283
sparser
"While EGF also activates Rac1, this effect is rapid and short lived."
21893191
sparser
"However, knockdown of EphA2 significantly suppressed the Rac1 activation by EGF ( xref )."
20679435
reach
"Thrombin and EGF respectively resulted in the activation of Rac1 in CHRF-288-11 cells and HeLa cells respectively while W7 inhibited the activation of Rac1 by thrombin and EGF."
22905193
reach
"Maximal Cdc42 and Rac1 activation in SKOV3ip cells treated with 10 ng/ml EGF occurred at different times; 20 min for Cdc42 and 5 min for Rac1 (XREF_SUPPLEMENTARY)."
26206334
reach
"However, further Rac1 activation by EGF was absent in CdGAP KD podocytes (Fig. 2D,E)."
36333327
sparser
"The present study clearly demonstrates that the activation of Rac1 by EGF plays an important role in the EGF-induced invasive phenotype in Hs578T and MDA-MB-231 cells."
21281602
reach
"Indeed, we found that centrosome amplification induced a consistent ~ 1.5-fold Rac1 activation using a biochemical pull-down assay to measure GTP liganded Rac1 in multiple cell lines [Note : maximal Rac1 activity induced by EGF in MCF10A cells is ~ 2-fold (XREF_FIG and XREF_SUPPLEMENTARY)]."
24739973
sparser
"To assess the involvement of Rac1 in the EGF-induced invasive phenotype of Hs578T and MDA-MB-231 cells, we first examined if EGF activated Rac1 in these cells."
21281602
reach
"Next, we examined the actions of CIIA variants on EGF induced Rac1 activation in 239T cells that had been transfected with vectors for HA-SOS1 and Flag tagged CIIA variants (CIIA, CIIA-DeltaC, and CIIA-DeltaN)."
22042618
reach
"Collectively, these results suggested that CIIA promoted the activation of Rac1 by EGF."
22042618
sparser
"Rac1 activation by EGF was inhibited by gefitinib, lapatinib or SB203580 but amplified by PD98059."
23411345
reach
"EGF induced activation of Rac1 and Cdc42 was increased in T+ but decreased in sT."
22592160
reach
"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively (28, 32)."
30541856
reach
"In cells, both compounds blocked Rac1 binding to its effector PAK1 following EGF induced Rac1 activation in a dose dependent manner, while showing no inhibition of the closely related Cdc42 and RhoA activity."
28410221
reach
"To clarify the role of each GEF, we time-lapse imaged the EGF induced activity change of Rac1 in A431 cells transfected with siRNA targeting each Rac1 GEF."
18653540
sparser
"An extensive functional analysis using P-Rex1 overexpressing breast cancer cell lines established the requirement of this GEF for Rac1 activation and cell migration induced by EGF, TGFα or HRG."
21893191
reach
"Consistent with these results, overexpression of WT HA-Cbl diminished whereas that of HA-Cbl-C3AHN enhanced the EGF induced Rac1 activation (XREF_FIG, A and B)."
20940296
reach
"The results showed that Rac1 activation was largely abolished in cells expressing GEP100 siRNA, GEP100-^ PH and Arf6-T27N, respectively, indicating that the GEP100 and Arf6 signaling pathway is essential for EGF stimulated Rac1 activation (XREF_FIG)."
22701712
sparser
"In search of a mechanism for the contribution of RhoG to the malignant behavior of glioblastoma cells, we found that depletion of RhoG strongly inhibits activation of the Rac1 GTPase by both HGF and EGF."
22966858
trips
"Coactivation of Rac1 and Cdc42 at lamellipodia and membrane ruffles induced by epidermal growth factor."
14699061
sparser
"Because knockdown of these GEFs suppressed EGF-induced Rac1 activation only partially, we looked for another Rac1 GEF downstream of the EGF receptor and found that Asef, a Rac1-Cdc42 GEF bound to the tumor suppressor APC, also contributed to EGF-induced Rac1 activation."
18653540
sparser
"Similar to BDNF, our results show that miR-204 inhibits EGF-induced activation of Rac1."
23285024
sparser
"We reported that EGF and the ErbB3 ligand heregulin β1 (HRG) strongly activate Rac1 in MCF-7 and T-47D breast cancer cells [ xref ]."
21172654
reach
"In conditions where cells were depleted of ARF1, Rac1 and IRSp53 remained in the cytosol even when cells were treated with EGF."
23707487
reach
"Altogether, these results argue for the requirement of a PLCgamma dependent activation of PKCepsilon for the activation of Rac1 by EGF."
22384062
reach
"PKG II Inhibits EGF induced Activation of RAC1."
23613900
sparser
"Even though no study has attempted to analyze comprehensively all these GEFs simultaneously and their interplay, it seems likely that Rac1 activation by EGF is controlled in a cell type- and cell function-dependent manner through these different GEFs."
20237158
sparser
"Furthermore, activation of Rac1 by EGF is inhibited by a PLC inhibitor."
12586341
reach
"FIP-fve inhibited EGF induced activation of Rac1."
24274472
reach
"MLS000532223 effectively inhibited EGF stimulated activation of Rac1 at 3-10 muM, while NSC23766 showed more modest inhibition even at doses up to 50 muM."
20008126
reach
"Epidermal growth factor (EGF) is an efficient activator of Rac1,2, and Cdc42 XREF_BIBR."
22509380
reach
"Both EGF and HGF promoted the activation of the Rac1 effector PAK in A549 cells."
34731623
reach
"The result showed that EGF treatment caused an obvious increase of active RAC1 and high activity of PKG II efficiently inhibited the activation of RAC1 (XREF_FIG)."
23613900
sparser
"Replacement of these two residues with alanine in a double mutant (K153A/R163A) strongly diminishes EGF-induced Rac1 activation [ xref ]."
32456244
reach
"Cell based measurement of GTPase activity demonstrated that R-ketorolac specifically inhibits epidermal growth factor stimulated Rac1 and Cdc42 activation at low micromolar concentrations."
26071482
reach
"This interaction is critical for EGF induced Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro."
19264842
reach
"Moreover, for EGF induced Rac1 activation, a step essential to F-actin formation and subsequent macropinocytosis, S1P signaling is required for its full activation, as judged by FRET analysis."
33431782
sparser
"Furthermore, transfection of GEP100-△PH, inhibition GEP100 or Arf6 activity by GEP100 siRNA or Arf6 T27N failed to facilitate EGF-induced ERK and Rac1 activation."
22701712
reach
"Moreover, silencing of ARF6 decreased by six-fold the level of GTP : Rac1 both in non stimulated condition and in response to EGF stimulation and abolished EGF induced Rac1 activation (XREF_FIG)."
25799492
reach
"Even though no study has attempted to analyze comprehensively all these GEFs simultaneously and their interplay, it seems likely that Rac1 activation by EGF is controlled in a cell type- and cell function dependent manner through these different GEFs."
20237158
reach
"Cell based measurement of GTPase activity (in animal and human cell lines) demonstrated that the R-enantiomers specifically inhibit epidermal growth factor stimulated Rac1 and Cdc42 activation."
26558612
reach
"This Rac1 activation by CIIA *-Myc in the cells was abrogated by RNAi mediated depletion of EPS8, suggesting that a positive effect of CIIA on EGF induced Rac1 activation requires EPS8."
22042618
reach
"We previously reported that the constitutive degradation of an endosomal small GTPase RhoB by the CUL3/KCTD10 E3 complex functions in EGF-induced Rac1 activation specifically in HER2-positive breast cancer cell lines, among other breast cancer subtypes (Murakami et al, 2019)."
34187934
sparser
"However, in our study, blocking ERK activity significantly prevents EGF-induced Rac1 activation."
22701712
sparser
"Recently, Arnst et al ( xref ) demonstrated two new compounds, referred in their publication as #1 and #6, that were design to target the nucleotide-binding site of Rac1 although, were able to block active Rac1 from binding to its effector PAK1, following EGF-induced Rac1 activation in a dose-dependent manner, they showed no inhibition of Cdc42 or RhoA."
28848995
reach
"Additionally, we showed that Rac1 activation by EGF was prevented by addition of an Akt inhibitor."
27102434
reach
"XREF_FIG shows, that 20 ng/mL EGF caused a 2.4-fold Rac1 activation after 5 min that was completely inhibited by preincubation with 50 muM RV for 30 min."
21732534
sparser
"In the present study, it was found that EGF‐induced Rac1 activation in vivo and in vitro ."
26769343
sparser
"We suggest that EGF-induced activation of Rac1 causes the activation of mTORC2 via RICTOR."
31995655
reach
"EGF was shown to activate the small GTPases Rac1 and Cdc42 at the leading edge XREF_BIBR and Rac1 activation was shown to be downstream of MAPK pathway upon EGF stimulation in the formation of lamellipodia XREF_BIBR."
25976978
sparser
"Moreover, silencing of ARF6 decreased by six-fold the level of GTP:Rac1 both in non-stimulated condition and in response to EGF stimulation and abolished EGF-induced Rac1 activation ( xref )."
25799492
reach
"Here, we confirm these findings by showing that overexpression of e3B1 in another fibroblastic cell line, NIH3T3 and EGFR, sensitizes EGF induced activation of Rac1."
16182283
reach
"Consistent with these results, RNAi mediated depletion of CIIA blocked EGF induced Rac1 activation in HeLa cells, and this effect was reversed by ectopic expression of CIIA *-Myc (XREF_FIG), which contains three silent point mutations within the region targeted by the CIIA siRNA."
22042618
sparser
"Moreover, EGF-induced Rac1 activation was promoted by Erk1/2 inhibition but repressed by p38MAPK inhibition."
23411345
reach
"Phosphorylation of threonine 108 in RAC1 by activated ERK, upon epidermal growth factor (EGF) treatment, also leads to increased nuclear RAC1 [34]."
29723737
reach
"RhoG mediates EGF stimulated Rac1 activation."
22966858
reach
"The preceding results indicate that Rac1 and Cdc42 are stimulated by EGF, but do not directly link their activity to ruffling and macropinocytosis."
20156964
reach
"In addition, in MDA-MB-231 cells, EGF stimulation increased both the activation status of Rac1 and RhoA; fetal bovine serum (FBS) stimulation increased the activation of RhoA, whereas deletion of IBP attenuated this activation."
23975422
reach
"Thus, our study is the first to demonstrate the involvement of CysLT1 signaling in the mechanism of the second wave of EGF induced Rac1 activation."
24350867
reach
"Ten minutes after EGF stimulation, the FRET signal became prominent at membrane ruffles at the dorsal cell surface, suggesting that EGF dependent activation of Rac1 occurred transiently and locally in SKBR-3 cells."
30515933
sparser
"Consistent with these observations, while CD99CRIII3 suppressed the EGF-induced activation of Rac1 and RhoA GTPases, its inhibitory effect was neutralized by siRNA-mediated knockdown of PTPN12 in MCF-7 cells ( xref E)."
33050232
sparser
"The EGF-induced activation of Rac1 in cells exogenously expressing ILK and ELMO2 suggests Rac1 may mediate ILK/ELMO2 modulation of cell polarization."
22160594
reach
"These two waves of lamellipodia formation were reflected by two waves of EGF induced Rac1 activation, which were observed at 2-5min and 6-12h, respectively."
24350867
reach
"Moreover, it is known that the EGF signaling pathway activates Rac1 and Cdc42 XREF_BIBR, and the IL-4 signaling pathway induces activation of the Rac1, Cdc42, and Wnt signaling pathways XREF_BIBR."
30963125
sparser
"PKG II Inhibits EGF-induced Activation of RAC1."
23613900
reach
"This is illustrated by the fact that the concentration of EGF required to cause maximal activation of Rac1 was relatively low (5 ng/ml) in the NIH3T3 and EGFR-e 3B1 cells but was much higher (at least[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16182283
sparser
"Altogether, these results argue for the requirement of a PLCγ-dependent activation of PKCε for the activation of Rac1 by EGF."
22384062
sparser
"In previous studies, the carboxyl-terminus of Vav2, which contains the SH2 and SH3 domains, was suggested to play an autoinhibitory role in regulating Vav2 activity, acting as a dominant negative for EGF-induced Rac1 activation ( xref )."
23615439
sparser
"We therefore examined this potential mode of action, and found that EGF-induced Rac1 activation was regulated by CysLT1 signaling (unpublished data)."
23139868
reach
"S100A7 overexpression affect EGF induced Rac1 activation and associated signaling."
23535840
sparser
"Basal and EGF induced activation of Rac1 in PC3 and DU145 cells were not affected in cells after Giα2 knockdown."
30078221
reach
"On the other hand, EGF phosphoinositide 3-kinase independently induces more transient Rac1 activation but neither accumulates active Rac1 nor forms Rac1- and filamentous actin rich protrusions."
11278419
reach
"CIIA and CIIA-DeltaC potentiated the EGF induced stimulation of Rac1 activity, whereas CIIA-DeltaN did not."
22042618
reach
"In cells clamped at pH 7.8, both Rac1 and Cdc42 were stimulated by EGF (XREF_FIG), as found earlier."
20156964
reach
"Furthermore, blocking ERK signaling with its inhibitor U0126 remarkably inhibited both EGF induced Rac1 activation as well as cell migration, and ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) also largely abolished EGF induced cell migration."
22701712
reach
"The ruffling observed in SNV- treated cells is similar to that seen in response to EGF mediated activation of Rac1 dependent ruffling [XREF_BIBR]."
23928044
sparser
"Rac1-GTP was transiently activated by EGF in G-LISA experiments, as already described by other authors [ xref ]."
33167372
sparser
"Concurrent activation of Rac1 and Cdc42 at lamellipodia and membrane ruffles by EGF has been reported in lung cancer cells, 42,43 which was probably achieved by phosphorylation and activation of Asef[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23562274
sparser
"We also showed that the extent of EGF-induced Rac1 activation in MDCK/CIIA-Flag cells was markedly greater than that in MDCK/control cells ( xref )."
22042618
reach
"Incubation of PANC-1 cells with either phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 or Src family kinases inhibitor pyrazolopyrimidine (PP2), attenuated EGF stimulated Rac1 activation from [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19116140
sparser
"3.4 EGF-induced Rac1 activation is abolished by RV treatment."
21732534
reach
"EGF also increased ERK and Rac1 activity."
22701712
reach
"While EGF also activates Rac1, this effect is rapid and short lived."
21893191
reach
"Furthermore, it was found that the activation of Rac1 by EGF stimulation of cells stably expressing QM992 was inhibited by U73122."
12586341
sparser
"G-LISA Rac1 activation assay demonstrated that p38MAPK inhibition (and EGFR tyrosine kinase inhibition) or Erk1/2 inhibition respectively decreased or increased EGF-induced Rac1 activation ( Fig. 7 C)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23411345
sparser
"In the present study, we demonstrate that MEK activation by EGF increased Rac1 activation, dissociation of intercellular contacts, and migration in both control and polyamine-depleted cells, while U0126, a specific inhibitor of MEK1, prevented disruption of junctions as well as EGF-induced Rac1 activation."
17183546
sparser
"To evaluate the ability of ZINC69391 to interfere the Rac1 activation by EGF, pull down assays were carried out in serum-starved F3II cells after the stimulation with EGF."
24066799
reach
"Here, we show that depletion of RhoG significantly inhibits both HGF- and EGF stimulated Rac1 activation in glioblastoma cells, demonstrating that RhoG acts upstream of Rac1 in these cells."
22966858
sparser
"Here, we confirm these findings by showing that overexpression of e3B1 in another fibroblastic cell line, NIH3T3/EGFR, sensitizes EGF-induced activation of Rac1."
16182283
sparser
"As shown in xref , the basal activity of Rac1 was not decreased after knockdown of endogenous Giα2 in PC3 and DU145 cells and the EGF induced activation of Rac1 was slightly decreased in both cell types which was not statistically significant."
30078221
reach
"In parental A549 cells, EGF caused a time-dependent activation of Rac1 at peripheral protrusions in a PI3K-dependent manner (Figure S4D; videos in Data S1)."
34731623
sparser
"This EGF-induced Rac1 activation was blocked by AG1478-mediated inhibition of the EGFR ( Fig. 2 A and B)."
19116140
reach
"Immunoblot analysis revealed that basal active Rac1 and RhoA levels were significantly decreased in Arl8b KD cells compared to NT cells (XREF_SUPPLEMENTARY), although EGF stimulated Rac1 and RhoA activity was not significantly different in Arl8b KD cells (XREF_SUPPLEMENTARY)."
27105540
reach
"More recently, Chang et al. demonstrated that in addition to PVF and EGF driven upregulation of Rac1, the formation of polarized protrusions in the leader cell is supported by negative regulation of the Hippo pathway by Rap1 [XREF_BIBR]."
31404788
reach
"Furthermore, activation of Rac1 by EGF is inhibited by a PLC inhibitor."
12586341
sparser
"Consequently, we examined whether ILK/ELMO2 species can modulate EGF-induced Rac1 activation in epidermal keratinocytes."
22160594
sparser
"The pull-down assay of active Rac1 showed that MLS000532223 completely inhibited EGF induced Rac1 activation in Swiss 3T3 cells."
20008126
reach
"In this study, we found that EGF treatment could activate Ras related C3 botulinum toxin substrate 1 (Rac1), PI3K and Akt and p21 actived kinase (PAK1) along with cell migration."
23554696
sparser
"Collectively, these results suggested that CIIA promoted the activation of Rac1 by EGF."
22042618
sparser
"MCF-7 cells were stimulated with EGF and IGF-I, which activate Rac1 in a P-Rex1-dependent manner [ xref ], as well as with 10% FBS."
29983884
reach
"Basal- and EGF induced activation of Rac1 in PC3 and DU145 cells were not affected in cells after Gialpha2 knockdown."
30078221
reach
"Our data provide evidence for EGF induced Rac1 activation and implicate PAK and JNK as downstream targets of Rac1 in EGF signal transduction in VSMC."
17350025
sparser
"Following treatment, both compound #1 (Figure xref ) and #6 (Figure xref ) diminished the EGF-induced Rac1 activation in a dose-dependent manner, with a nearly complete inhibition observed at 50 μM for both compounds."
28410221
reach
"Recently, Arnst et al demonstrated two new compounds, referred in their publication as # 1 and # 6, that were design to target the nucleotide binding site of Rac1 although, were able to block active Rac1 from binding to its effector PAK1, following EGF induced Rac1 activation in a dose dependent manner, they showed no inhibition of Cdc42 or RhoA."
28848995
reach
"Because we established that Rac1 stimulation could rescue a normal cyst phenotype for MDCK ARF6si cysts, we sought to determine whether Rac1 activation by CN02 and EGF treatment could also restore the supportive laminin-111 microenvironment."
23051733
reach
"EGF and TGFalpha, but not Fnf, increased Rac1 activity and promoted the increase in MMP-13 above that stimulated by Fnf alone."
23460186
reach
"Previously we reported that EGF induced distinct waves of Rac1 activation, and that the second wave Rac1 activation regulated the second wave of lamellipodia formation."
24350867
sparser
"Vav2 Is Critical for EGF-induced Rac1 and Cdc42 Activation, Reorganization of Junctional Actin Cytoskeleton, and Cell Migration in MECs."
20940296
reach
"Vav2 Is Critical for EGF induced Rac1 and Cdc42 Activation, Reorganization of Junctional Actin Cytoskeleton, and Cell Migration in MECs."
20940296
reach
"EGF and TGFalpha, but not FN fragments, increased Rac1 activity and promoted the increase in MMP-13 above that achieved by stimulation with FN fragments alone."
23460186
sparser
"Remarkably, FRET experiments determined that silencing any of the three GEFs abolished EGF-dependent Rac1 activation ( xref ; videos in xref )."
34731623
sparser
"Ectopic expression of PPM1B results in dephosphorylation of RhoGDI1 and, thereby, abates the activation of RhoA, Rac1 and CDC42 by epidermal growth factor (EGF)."
29307615
reach
"In a similar fashion, EGF caused activation of Rac1 in HeLa cells and this activation was also blocked in the presence of W7 (XREF_FIG)."
22905193
sparser
"Pretreatment of VSMC by EGF receptor specific tyrosine kinase inhibitor AG1478 and non-specific tyrosine kinase inhibitor genistein inhibited EGF-induced activation of Rac1, PAK and JNK, whereas tyrosine kinase inhibitors specific for Src (PP1) and specific for platelet-derived growth factor (AG1296) had no effect."
17350025
reach
"The pull-down assay of active Rac1 showed that MLS000532223 completely inhibited EGF induced Rac1 activation in Swiss 3T3 cells."
20008126
sparser
"In cells, both compounds blocked Rac1 binding to its effector PAK1 following EGF-induced Rac1 activation in a dose-dependent manner, while showing no inhibition of the closely related Cdc42 and RhoA activity."
28410221
reach
"G-LISA Rac1 activation assay demonstrated that p38MAPK inhibition (and EGFR tyrosine kinase inhibition) or Erk1/2 inhibition respectively decreased or increased EGF induced Rac1 activation."
23411345
sparser
"EGF activates RhoA and Rac1 through activation of the EGF pathway [ xref , xref ]."
23721065
sparser
"Furthermore, blocking ERK signaling with its inhibitor U0126 remarkably inhibited both EGF-induced Rac1 activation as well as cell migration, and ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) also largely abolished EGF-induced cell migration."
22701712
reach
"Depletion of ARF1 significantly inhibited EGF dependent activation of Rac1."
23707487
reach
"We examined whether EGF activated Rac1 and cdc42 is regulated by fibronectin expression."
25797258
reach
"The present study clearly demonstrates that the activation of Rac1 by EGF plays an important role in the EGF induced invasive phenotype in Hs578T and MDA-MB-231 cells."
21281602
reach
"betaPix siRNA based knockdown experiments block EGF induced H 2 O 2 production and Rac1 activation."
23639002
reach
"Our finding that RhoG contributes to Rac1 activation downstream of EGFR in glioblastoma cells however, contrasts to published observations that EGF stimulated Rac1 activation is independent of RhoG in HeLa cells [XREF_BIBR]."
22966858
reach
"The signaling of EGF activates RAC1 to control cytoskeletal reorganization, a crucial event in the metastatic cascade 19."
33633298
sparser
"RNAi-mediated depletion of SOS1 suppressed the EGF-induced activation of Rac1 in HeLa cells ( xref ), showing that SOS1 mediates Rac1 activation by this growth factor."
22042618
sparser
"Down-regulation of Asef by siRNA inhibits the EGF-induced activation of Rac1."
30544910
reach
"EGF induced an increase in Rac1 activity that persisted for the first 10-15 min of EGF stimulation, coincident with the Arp2/3 complex binding to vinculin (XREF_FIG A)."
12473693
reach
"Additionally, the activation of the planar cell polarity proteins PTK7 (protein tyrosine kinase 7), CELSR (Cadherin EGF LAG seven-pass G-type receptor), and VANG (Van Gogh like) by Wnts or Fzd receptors may support PCP GTPase signaling by recruiting the Rac1 and Cdc42 GEF PAK, the Rac1 GEF ARHGEF7, and the ARF GAP GIT1 [XREF_BIBR, XREF_BIBR]."
27589803