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EGF activates RAC1. 309 / 344
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"Epidermal growth factor stimulates Rac1 activation through Src and PI3K to promote colonic epithelial cell migration XREF_BIBR and induces the ERK and Rac1 signaling pathway to promote cell migration in human hepatoma HepG2 cells XREF_BIBR."
26078726
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"The pull-down assay of active Rac1 showed that MLS000532223 completely inhibited EGF induced Rac1 activation in Swiss 3T3 cells."
20008126
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"UTKO1 inhibited EGF induced Rac1 activation at 12 hrs with the same concentration to inhibit lamellipodia formation."
30247093
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"Here, we confirm these findings by showing that overexpression of e3B1 in another fibroblastic cell line, NIH3T3/EGFR, sensitizes EGF-induced activation of Rac1."
16182283
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"FIP-fve inhibited EGF induced activation of Rac1."
24274472
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"Asef phosphorylation at Tyr94 plays an essential role in the EGF induced activation of Rac1 and Cdc42 and functions in an Src dependent manner in A431 cells [4]."
24378532
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"However, knockdown of EphA2 significantly suppressed the Rac1 activation by EGF ( xref )."
20679435
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"In addition, in MDA-MB-231 cells, EGF stimulation increased both the activation status of Rac1 and RhoA; fetal bovine serum (FBS) stimulation increased the activation of RhoA, whereas deletion of IBP attenuated this activation."
23975422
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"In search of a mechanism for the contribution of RhoG to the malignant behavior of glioblastoma cells, we found that depletion of RhoG strongly inhibits activation of the Rac1 GTPase by both HGF and EGF."
22966858
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"MCF-7 cells were stimulated with EGF and IGF-I, which activate Rac1 in a P-Rex1-dependent manner [XREF_BIBR], as well as with 10% FBS."
29983884
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"In the present study, it was found that EGF induced Rac1 activation in vivo and in vitro."
26769343
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"While EGF also activates Rac1, this effect is rapid and short lived."
21893191
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"Therefore, it may be reasonable to believe that EGF induced activation of Rac1 and enhanced the expression of TJ and AJ proteins mediated through PI3K and Akt signalling pathway."
26769343
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"G-LISA Rac1 activation assay demonstrated that p38MAPK inhibition (and EGFR tyrosine kinase inhibition) or Erk1/2 inhibition respectively decreased or increased EGF-induced Rac1 activation ( Fig. 7 C)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23411345
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"Moreover, EGF-induced Rac1 activation was promoted by Erk1/2 inhibition but repressed by p38MAPK inhibition."
23411345
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"EGF is known to stimulate both Rac1 and Pak1 in vascular smooth muscle cells (Figure 5) [89]."
35740379
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"EGF and HRG also activate Rac1 in other breast cancer cell lines as well as in immortalized MCF-10A mammary cells (XREF_SUPPLEMENTARY)."
21172654
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"Both EGF and HGF can induce several Rac1 or Cdc42 specific Guanine nucleotide exchange factors (GEFs) [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]; however, it is still unclear which RacGEF (s) are responsible for MLK3 activation."
27213454
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"Interestingly, EGF exclusively led to Rac1 activation in singles, while in leaders we observed a gradual deactivation of Rac1 (Fig. S4 B)."
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"Dock4-KO cells showed significant inhibition of EGF-induced activation of Rac1 (Fig.  xref a), while the expression levels of Rac1 protein were not changed (Fig.  xref b,c)."
38802496
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"Further studies indicate that epidermal growth factor (EGF) signaling activates Rac-1, which is involved in cell migration, by triggering the formation of the ternary complex of Ras-related C3 botulin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26456530
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"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively ( xref , xref )."
30541856
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"Depletion of ARF1 significantly inhibited EGF-dependent activation of Rac1 ( Fig. 2 B)."
23707487
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"RNAi mediated depletion of SOS1 suppressed the EGF induced activation of Rac1 in HeLa cells (XREF_FIG), showing that SOS1 mediates Rac1 activation by this growth factor."
22042618
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"An extensive functional analysis using P-Rex1 overexpressing breast cancer cell lines established the requirement of this GEF for Rac1 activation and cell migration induced by EGF, TGFα or HRG."
21893191
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"Epidermal growth factor (EGF) is an efficient activator of Rac1,2, and Cdc42 XREF_BIBR."
22509380
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"The effects of Rac1 activation by EGF, and deactivation by Rac1 inhibitor NSC23766, in OECM1 & SAS spheroids are given in Fig. S4 ."
30500449
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"Next, we examined the actions of CIIA variants on EGF induced Rac1 activation in 239T cells that had been transfected with vectors for HA-SOS1 and Flag tagged CIIA variants (CIIA, CIIA-DeltaC, and CIIA-DeltaN)."
22042618
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"EGF activates RhoA and Rac1 through activation of the EGF pathway [ xref , xref ]."
23721065
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"CIIA and CIIA-DeltaC potentiated the EGF induced stimulation of Rac1 activity, whereas CIIA-DeltaN did not."
22042618
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"EGF induced activation of Rac1 and Cdc42 was increased in T+ but decreased in sT."
22592160
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"EGF signaling triggers activation of RAC1 and CDC42, which link the signal to the actin cytoskeleton leading to the formation of lamellipodia- and filopodia-like protrusions, respectively (Figure 2A; Kurokawa et al., 2004)."
37057894
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"We also showed that the extent of EGF induced Rac1 activation in MDCK and CIIA-Flag cells was markedly greater than that in MDCK and control cells (XREF_FIG)."
22042618
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"Consistent with these results, RNAi mediated depletion of CIIA blocked EGF induced Rac1 activation in HeLa cells, and this effect was reversed by ectopic expression of CIIA *-Myc (XREF_FIG), which contains three silent point mutations within the region targeted by the CIIA siRNA."
22042618
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"However, further Rac1 activation by EGF was absent in CdGAP KD podocytes (Fig. 2D,E)."
36333327
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"EGF also increased ERK and Rac1 activity."
22701712
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"Coactivation of Rac1 and Cdc42 at lamellipodia and membrane ruffles induced by epidermal growth factor."
14699061
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"This Rac1 activation by CIIA *-Myc in the cells was abrogated by RNAi mediated depletion of EPS8, suggesting that a positive effect of CIIA on EGF induced Rac1 activation requires EPS8."
22042618
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"Furthermore, blocking ERK signaling with its inhibitor U0126 remarkably inhibited both EGF induced Rac1 activation as well as cell migration, and ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) also largely abolished EGF induced cell migration."
22701712
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"As lamellipodia formation has been reported to be mainly regulated by Rac1, 27,28 we also found that EGF stimulation induced rapid and transient activation of Rac1 at 2–5 min and a second wave of Rac1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27094149
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"The regulatory mechanisms and role of early EGF-induced Rac1 activation have been extensively studied, 29,30 however, the mechanism by which the EGF-induced second wave of Rac1 activation and lamellip[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27094149
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"Therefore, we anticipated that the identification of a 7 -binding molecule would provide information about the regulatory mechanism behind the EGF-induced second wave of Rac1 activation and lamellipod[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27094149
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"Collectively, these results suggested that CIIA promoted the activation of Rac1 by EGF."
22042618
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"Immunoblot analysis revealed that basal active Rac1 and RhoA levels were significantly decreased in Arl8b KD cells compared to NT cells (XREF_SUPPLEMENTARY), although EGF stimulated Rac1 and RhoA activity was not significantly different in Arl8b KD cells (XREF_SUPPLEMENTARY)."
27105540
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"Since the expression level of Tiam1 was quite low in un-stimulated A431 cells, it is unlikely that Tiam1 is responsible for the EGF-induced first wave of Rac1 activation."
27094149
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"This result suggested that EGF induced Rac1 activation may occur transiently and/or locally in SKBR-3 cells and, thus, it might be difficult to detect Rac1-GTP from total cell lysates by the pull-down assay."
30515933
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"Ten minutes after EGF stimulation, the FRET signal became prominent at membrane ruffles at the dorsal cell surface, suggesting that EGF dependent activation of Rac1 occurred transiently and locally in SKBR-3 cells."
30515933
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"Analysis of Rac1 biological activity showed that Rac1N17 lentivirus particles infection significantly inhibited epidermal growth factor stimulated Rac1 activity in the PFCs, while Rac1L61 lentivirus particles enhanced the Rac1 activity."
20159679
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"EGF induced an increase in Rac1 activity that persisted for the first 10-15 min of EGF stimulation, coincident with the Arp2/3 complex binding to vinculin (XREF_FIG A)."
12473693
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"Interestingly, EGF was unable to reactivate Rac1 in Ba/F3p210S509A cells under the imatinib + SKF-96365 conditions (Supplementary Figure S4b), indicating the necessity of RhoA activation by BCR-ABL prior to observing the effects of SKF-96365 (Supplementary Figure S2)."
39199564
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"EGF-induced Rac1 activation and EGF-guided chemotactic migration of HSC3 cells were both VAV2 and clathrin dependent."
39744818
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"Basal and EGF induced activation of Rac1 in PC3 and DU145 cells were not affected in cells after Giα2 knockdown."
30078221
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"As shown in xref , the basal activity of Rac1 was not decreased after knockdown of endogenous Giα2 in PC3 and DU145 cells and the EGF induced activation of Rac1 was slightly decreased in both cell types which was not statistically significant."
30078221
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"Cell based measurement of GTPase activity (in animal and human cell lines) demonstrated that the R-enantiomers specifically inhibit epidermal growth factor stimulated Rac1 and Cdc42 activation."
26558612
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"Previously, we set up controls for this assay, such as a known model of Cdc42 and Rac1 activation by EGF in HeLa cells ( xref ( xref ), and GDP-loaded, and GTPγS-loaded HeLa protein extracts ( xref )."
25862245
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"EGF increases Rac1 activity 5- to 10-fold above basal levels with maximal activity evident at 2 min post-stimulation in Swiss 3T3 fibroblasts and HeLa cells."
26558612
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"Using GLISA, 300 muM R-naproxen pretreatment of Swiss 3T3 cells for 15 min significantly inhibited EGF mediated Rac1 activation relative to EGF stimulated controls and a 1 h pretreatment reduced activation to basal unstimulated levels (XREF_FIG)."
26558612
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"S1PR2 inhibition reduces EGF-induced Rac1 activity."
37038210
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"Importantly, Armus plays a key role in EGF induced scattering and Arf6 dependent disassembly of junctions, by integrating Rac1 activation with Rab7 function to promote E-cadherin degradation.By using [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20116244
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"This compound was able to block Rac1 interaction with its GEF Tiam1, prevented EGF induced Rac1 activation and inhibited cell proliferation, cell migration and cell cycle progression in highly aggressive breast cancer cell lines."
24066799
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"Intriguingly, DGKγ significantly attenuated the EGF-dependent Rac1 activation when co-expressed with β2-chimaerin ( Fig. 4 A)."
17254573
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"These results indicate that DGKγ inhibits EGF-induced Rac1 activation through enhancing β2-chimaerin Rac-GAP activity in vivo."
17254573
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"These results suggest that HER2 is essential for EGF induced Rac1 activation in SKBR-3 cells."
30515933
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"However, in our study, blocking ERK activity significantly prevents EGF induced Rac1 activation."
22701712
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"Furthermore, it was found that the activation of Rac1 by EGF stimulation of cells stably expressing QM992 was inhibited by U73122."
12586341
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"We reported that EGF and the ErbB3 ligand heregulin β1 (HRG) strongly activate Rac1 in MCF-7 and T-47D breast cancer cells [ xref ]."
21172654
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"EGF and HRG also activate Rac1 in other breast cancer cell lines as well as in immortalized MCF-10A mammary cells ( xref )."
21172654
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"Thrombin and EGF respectively resulted in the activation of Rac1 in CHRF-288-11 cells and HeLa cells respectively while W7 inhibited the activation of Rac1 by thrombin and EGF."
22905193
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"The results showed that Rac1 activation was largely abolished in cells expressing GEP100 siRNA, GEP100-^ PH and Arf6-T27N, respectively, indicating that the GEP100 and Arf6 signaling pathway is essential for EGF stimulated Rac1 activation (XREF_FIG)."
22701712
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"To confirm this observation using an alternative approach, EGF-induced Rac1 activity was shown to be strongly inhibited by RNAi-mediated knockdown of VAV2 (Fig. S5)."
39744818
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"Activation of Rac1 was caused by EGF/EGF receptors stimulation rather than via the S1P/S1PR2 axis, however to stimulate Rac1 activity, a signal from S1PR2 was required."
37038210
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"Together, Fig. 6D,E and Fig. S5 demonstrate that VAV2 is the key GEF promoting EGF-stimulated Rac1 activity in HSC3 cells.The requirement of CME for sustained VAV2-dependent Rac1 activation by EGF prompted us to test whether Rac1 is present in EGFR-containing endosomes in HSC3 cells."
39744818
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"In HSC3 cells, our data indicate that VAV2 is essential for EGF-induced Rac1 activation (Fig. 6D,E; Fig. S5) and has a major role in EGF-guided chemotactic cell migration (Fig. 4B,C)."
39744818
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"Additionally, the activation of the planar cell polarity proteins PTK7 (protein tyrosine kinase 7), CELSR (Cadherin EGF LAG seven-pass G-type receptor), and VANG (Van Gogh like) by Wnts or Fzd receptors may support PCP GTPase signaling by recruiting the Rac1 and Cdc42 GEF PAK, the Rac1 GEF ARHGEF7, and the ARF GAP GIT1 [XREF_BIBR, XREF_BIBR]."
27589803
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"PTX also inhibited the activation of Rac1 by EGF (data not shown)."
21172654
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"Thrombin or EGF resulted in activation of Rac1 in CHRF-288-11 or HeLa cells respectively and W7 inhibited this activation."
22905193
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"Thrombin and EGF respectively resulted in the activation of Rac1 in CHRF-288-11 cells and HeLa cells respectively while W7 inhibited the activation of Rac1 by thrombin and EGF."
22905193
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"In a similar fashion, EGF caused activation of Rac1 in HeLa cells and this activation was also blocked in the presence of W7 (XREF_FIG)."
22905193
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"EGF stimulation of wild type Rac1 or Rac1 (R163A) in HeLa cells led to the activation of Rac1 (XREF_FIG)."
22905193
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"Remarkably, FRET experiments determined that silencing any of the three GEFs abolished EGF-dependent Rac1 activation ( xref ; videos in xref )."
34731623
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"Rapid Activation of RhoG and Rac1 by EGF Is Not Interdependent."
20237158
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"These data demonstrate that ' hyper-stimulation ' of the EGF pathway, which induces Rac1 activation, is sufficient to promote the uptake of a non invasive C. jejuni strain."
21999233
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"We previously reported that the constitutive degradation of an endosomal small GTPase RhoB by the CUL3/KCTD10 E3 complex functions in EGF-induced Rac1 activation specifically in HER2-positive breast cancer cell lines, among other breast cancer subtypes (Murakami et al, 2019)."
34187934
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"This interaction is critical for EGF induced Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro."
19264842
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"Even though no study has attempted to analyze comprehensively all these GEFs simultaneously and their interplay, it seems likely that Rac1 activation by EGF is controlled in a cell type- and cell function-dependent manner through these different GEFs."
20237158
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"On the other hand, EGF phosphoinositide 3-kinase independently induces more transient Rac1 activation but neither accumulates active Rac1 nor forms Rac1- and filamentous actin rich protrusions."
11278419
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"Moreover, silencing of ARF6 decreased by six-fold the level of GTP:Rac1 both in non-stimulated condition and in response to EGF stimulation and abolished EGF-induced Rac1 activation ( xref )."
25799492
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"Vav2 Is Critical for EGF-induced Rac1 and Cdc42 Activation, Reorganization of Junctional Actin Cytoskeleton, and Cell Migration in MECs."
20940296
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"EGF activates RhoA and Rac1 through activation of the EGF pathway [XREF_BIBR, XREF_BIBR]."
23721065
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"Altogether, our data suggest that the brain endothelium and EGF contribute to extravasation by promoting DOCK4/RAC1- and DOCK9/CDC42-mediated elongation and intercalation of breast cancer cells."
38762624
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"Replacement of these two residues with alanine in a double mutant (K153A/R163A) strongly diminishes EGF-induced Rac1 activation [ xref ]."
32456244
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"To clarify the role of each GEF, we time-lapse imaged the EGF induced activity change of Rac1 in A431 cells transfected with siRNA targeting each Rac1 GEF."
18653540
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"Because knockdown of these GEFs suppressed EGF induced Rac1 activation only partially, we looked for another Rac1 GEF downstream of the EGF receptor and found that Asef, a Rac1-Cdc42 GEF bound to the tumor suppressor APC, also contributed to EGF induced Rac1 activation."
18653540
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"BU-4664L : In the process of further investigation into the mechanism by which EGF induces the second wave of Rac1 activation, BU-4664L [21,22], an inhibitor of 5-lipoxygenase (5-LOX), was found to inhibit the EGF induced second wave but not the first wave of Rac1 activation."
30247093
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"Furthermore, it has been reported that in 3D Matrigel culture, MDCK cells depleted of the small GTPase Arf6 form an inverted cyst, whose inversion is restored by epidermal growth factor‐elicited Rac1 activation and by expression of a constitutively active form of Rac1 (Monteleon et al., 2012)."
39377417
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"Previously, we reported that epidermal growth factor (EGF) induced two waves of Rac1 activation; namely, at 5min and 12h after stimulation."
24350867
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"Recently, Arnst et al demonstrated two new compounds, referred in their publication as # 1 and # 6, that were design to target the nucleotide binding site of Rac1 although, were able to block active Rac1 from binding to its effector PAK1, following EGF induced Rac1 activation in a dose dependent manner, they showed no inhibition of Cdc42 or RhoA."
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"RNAi-mediated depletion of SOS1 suppressed the EGF-induced activation of Rac1 in HeLa cells ( xref ), showing that SOS1 mediates Rac1 activation by this growth factor."
22042618
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"However, EGF stimulation causes Rac-1 mediated internalization of E-cadherin via macropinocytosis."
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"Consistent with these results, overexpression of WT HA-Cbl diminished whereas that of HA-Cbl-C3AHN enhanced the EGF-induced Rac1 activation ( xref , A and B )."
20940296
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"A second wave of EGF induced Rac1 activation was required for EGF induced cell migration, however, the spatiotemporal regulation of the second wave of EGF induced Rac1 activation remains largely unclear."
24350867
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"Altogether, these results argue for the requirement of a PLCgamma dependent activation of PKCepsilon for the activation of Rac1 by EGF."
22384062
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"Furthermore, blocking ERK signaling with its inhibitor U0126 remarkably inhibited both EGF-induced Rac1 activation as well as cell migration, and ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) also largely abolished EGF-induced cell migration."
22701712
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"Next, we examined the actions of CIIA variants on EGF-induced Rac1 activation in 239T cells that had been transfected with vectors for HA-SOS1 and Flag-tagged CIIA variants (CIIA, CIIA-ΔC, and CIIA-ΔN)."
22042618
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"We also showed that the extent of EGF-induced Rac1 activation in MDCK/CIIA-Flag cells was markedly greater than that in MDCK/control cells ( xref )."
22042618
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"This Rac1 activation by CIIA*-Myc in the cells was abrogated by RNAi-mediated depletion of EPS8, suggesting that a positive effect of CIIA on EGF-induced Rac1 activation requires EPS8."
22042618
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"In contrast, combined Cbl and Cbl-b KD dramatically enhanced the EGF-induced Vav2 phosphorylation in response to EGF stimulation ( xref G ) and enhanced the EGF-induced Rac1 and Cdc42 activation ( xref , A and B )."
20940296
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"However, upon RNAi depletion of Ect2 from A549 cells we could not find any significant changes in Rac1 activation induced by EGF (C.L.H. and M.G.K., unpublished studies)."
22384062
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"Expression of dominant negative Rac1 in A431 cells abolished Rac1 activation by EGF and prevented binding of the Arp2/3 complex to vinculin induced by EGF ( xref A)."
12473693
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"Collectively, these results suggested that CIIA promoted the activation of Rac1 by EGF."
22042618
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"We used HeLa and Swiss 3T3 cells for these studies because the robust Rac1 activation induced by EGF stimulation in these cells serves as an excellent positive control."
29746086
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"Inhibition of ERK activity by U0126 or suppression of Rac1 activity by ectopic expression of inactive mutant form of Rac1 (Rac1-T17N) significantly prevents EGF-induced cell migration, suggesting that EGF-induced ERK and Rac1 activation was responsible for the migration of these cancer cells."
22701712
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"Furthermore, transfection of GEP100-△PH, inhibition GEP100 or Arf6 activity by GEP100 siRNA or Arf6 T27N failed to facilitate EGF-induced ERK and Rac1 activation."
22701712
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"Therefore, it may be reasonable to speculate that EGF-induced ERK and Rac1 activation and cell migration require the PH domain of GEP100 and are mediated through the GEP100/Arf6 pathways."
22701712
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"However, in our study, blocking ERK activity significantly prevents EGF-induced Rac1 activation."
22701712
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"Activity of RAC1 increases by TGF-beta, but also by EGF, and both act synergistically to get maximum effects."
16331683
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"ZINC69391 Interferes Rac1 Activation by EGF."
24066799
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"We examined whether EGF activated Rac1 and cdc42 is regulated by fibronectin expression."
25797258
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"To evaluate the ability of ZINC69391 to interfere the Rac1 activation by EGF, pull down assays were carried out in serum-starved F3II cells after the stimulation with EGF."
24066799
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"These results therefore demonstrate that BRK colocalizes and alters the subcellular localization of KAP3A and the C-terminus of KAP3A is required for KAP3A localization.BRK has been shown to phosphory[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18077133
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"In the first transition, the ligand EGF binds EGFR and activates PI3K and RAC1 proteins, which reduce apical polarity induction in the early pancreatic progenitor epithelium, leading to endocrine commitment towards a β-cell fate."
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"Rac1 dissociation from RhoGDI and subsequent Rac1 activation induced by the growth factors EGF and PDGF required phosphorylation of S101 and S174 by Pak1."
16472651
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"Tiam1 expression is required for the second wave of EGF induced Rac1 activation in A431 cells."
24350867
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"We showed that ZINC69391 was able to impair Rac1 activation by EGF, a described activator of Rac1 signaling pathway [ xref ]."
24066799
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"Assessment of Rac1 activation by EGF using a p21-binding domain (PBD) pull-down assay in Rac-GEF KD cells revealed that only FARP1 depletion reduced Rac1-GTP levels significantly ( xref )."
34731623
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"These two waves of lamellipodia formation were reflected by two waves of EGF induced Rac1 activation, which were observed at 2-5min and 6-12h, respectively."
24350867
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"Since EGF-induced activation of Rac/Cdc42 has not been demonstrated in VSMC so far, we hypothesized that EGF-induced growth of VSMC might involve stimulation of Rac/Cdc42 and PAK.In the present report[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17350025
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"In order to understand role for Rac1 in IMC-C225 mediated inhibition of cell invasion, pull-down assays using the PBD of PAK were performed to measure Rac1 activation in MDA-MB468 cells treated with either EGF or with IMC-C225 for the same time points as studied for RhoA activation."
18829495
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"Moreover, it is known that the EGF signaling pathway activates Rac1 and Cdc42 XREF_BIBR, and the IL-4 signaling pathway induces activation of the Rac1, Cdc42, and Wnt signaling pathways XREF_BIBR."
30963125
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"In cells, both compounds blocked Rac1 binding to its effector PAK1 following EGF induced Rac1 activation in a dose dependent manner, while showing no inhibition of the closely related Cdc42 and RhoA activity."
28410221
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"Following treatment, both compound # 1 and # 6 diminished the EGF induced Rac1 activation in a dose dependent manner, with a nearly complete inhibition observed at 50 muM for both compounds."
28410221
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"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively (28, 32)."
30541856
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"Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) significantly activated intracellular Rac1 and Cdc42, respectively (28, 32)."
30541856
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"In addition, emodin markedly suppressed EGF-induced activation of Cdc42 and Rac1 and the corresponding cytoskeleton changes."
15928809
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"Indeed, we found that centrosome amplification induced a consistent ~ 1.5-fold Rac1 activation using a biochemical pull-down assay to measure GTP liganded Rac1 in multiple cell lines [Note : maximal Rac1 activity induced by EGF in MCF10A cells is ~ 2-fold (XREF_FIG and XREF_SUPPLEMENTARY)]."
24739973
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"Consequently, we examined whether ILK and ELMO2 species can modulate EGF induced Rac1 activation in epidermal keratinocytes."
22160594
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"Exogenous expression of ILK and/or ELMO2 significantly enhanced EGF induced Rac1 activation over the same period (XREF_FIG and XREF_FIG)."
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"Furthermore, specific downregulation of PI3K/Akt signaling in MDA-MB-231 cells did not alter EGF-induced activation of Rac1."
23554696
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"The EGF induced activation of Rac1 in cells exogenously expressing ILK and ELMO2 suggests Rac1 may mediate ILK and ELMO2 modulation of cell polarization."
22160594
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"Previously we reported that EGF induced distinct waves of Rac1 activation, and that the second wave Rac1 activation regulated the second wave of lamellipodia formation."
24350867
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"EGF was shown to activate the small GTPases Rac1 and Cdc42 at the leading edge XREF_BIBR and Rac1 activation was shown to be downstream of MAPK pathway upon EGF stimulation in the formation of lamellipodia XREF_BIBR."
25976978
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"As shown in XREF_FIG (left panel), EGF stimulated Rac1 activity was significantly suppressed (XREF_FIG (right panel) in Rab5C KD cells."
24587345
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"MLS000532223 effectively inhibited EGF stimulated activation of Rac1 at 3-10 muM, while NSC23766 showed more modest inhibition even at doses up to 50 muM."
20008126
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"Indeed, this concept is supported by a previous study showing that EGF treatment of MCF-7 and MDA-MB-231 breast cancer cells promotes the release and activation of RhoA, Rac1 and Cdc42 by Ser174 phosp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29307615
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"EGF stimulated Rac1 activation is rapid and transient."
24587345
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"However, our results show that EGF treatment increases the activation of RhoA and Cdc42 as well as Rac1, and this event is suppressed by PPM1B in human breast cancer cell lines."
29307615
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"In previous studies, the carboxyl-terminus of Vav2, which contains the SH2 and SH3 domains, was suggested to play an autoinhibitory role in regulating Vav2 activity, acting as a dominant negative for EGF-induced Rac1 activation ( xref )."
23615439
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"3.4 EGF-induced Rac1 activation is abolished by RV treatment."
21732534
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"The pull-down assay of active Rac1 showed that MLS000532223 completely inhibited EGF induced Rac1 activation in Swiss 3T3 cells."
20008126
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"S100A7 overexpression affect EGF-induced Rac1 activation and associated signaling."
23535840
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"Rac1-GTP was transiently activated by EGF in G-LISA experiments, as already described by other authors [ xref ]."
33167372
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"In cells, both compounds blocked Rac1 binding to its effector PAK1 following EGF-induced Rac1 activation in a dose-dependent manner, while showing no inhibition of the closely related Cdc42 and RhoA activity."
28410221
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"RhoG mediates EGF stimulated Rac1 activation."
22966858
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"In addition, depletion of RhoG significantly inhibits EGF stimulated Rac1 activation in these cells."
22966858
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"It was shown that MLS000532223 inhibited EGF-stimulated Rac1 activation and EGF-stimulated stress fibre formation in mouse embryonic fibroblasts."
21262381
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"In the present study, we demonstrate that MEK activation by EGF increased Rac1 activation, dissociation of intercellular contacts, and migration in both control and polyamine depleted cells, while U0126, a specific inhibitor of MEK1, prevented disruption of junctions as well as EGF induced Rac1 activation."
17183546
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"Following treatment, both compound #1 (Figure xref ) and #6 (Figure xref ) diminished the EGF-induced Rac1 activation in a dose-dependent manner, with a nearly complete inhibition observed at 50 μM for both compounds."
28410221
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"Here, we show that depletion of RhoG significantly inhibits both HGF- and EGF stimulated Rac1 activation in glioblastoma cells, demonstrating that RhoG acts upstream of Rac1 in these cells."
22966858
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"These data suggest that EGF acting through MEK1 and ERK to activate Rac1 regulates cell-cell contacts."
17183546
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"Our finding that RhoG contributes to Rac1 activation downstream of EGFR in glioblastoma cells however, contrasts to published observations that EGF stimulated Rac1 activation is independent of RhoG in HeLa cells [XREF_BIBR]."
22966858
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"Phosphorylation of threonine 108 in RAC1 by activated ERK, upon epidermal growth factor (EGF) treatment, also leads to increased nuclear RAC1 [34]."
29723737
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"Therefore EGF induced activation of Rac1 promotes T-cad relocalization downstream of EGFR phosphorylation."
23411345
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"G-LISA Rac1 activation assay demonstrated that p38MAPK inhibition (and EGFR tyrosine kinase inhibition) or Erk1/2 inhibition respectively decreased or increased EGF induced Rac1 activation."
23411345
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"Thus, our study is the first to demonstrate the involvement of CysLT1 signaling in the mechanism of the second wave of EGF induced Rac1 activation."
24350867
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"Moreover, for EGF induced Rac1 activation, a step essential to F-actin formation and subsequent macropinocytosis, S1P signaling is required for its full activation, as judged by FRET analysis."
33431782
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"Similar to BDNF, our results show that miR-204 inhibits EGF induced activation of Rac1."
23285024
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"Maximal Cdc42 and Rac1 activation in SKOV3ip cells treated with 10 ng/ml EGF occurred at different times; 20 min for Cdc42 and 5 min for Rac1 (XREF_SUPPLEMENTARY)."
26206334
sparser
"Similar to BDNF, our results show that miR-204 inhibits EGF-induced activation of Rac1."
23285024
sparser
"In the present study, it was found that EGF‐induced Rac1 activation in vivo and in vitro ."
26769343
sparser
"Therefore, it may be reasonable to believe that EGF‐induced activation of Rac1 and enhanced the expression of TJ and AJ proteins mediated through PI3K/Akt signalling pathway."
26769343
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"Incubation of PANC-1 cells with either phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 or Src family kinases inhibitor pyrazolopyrimidine (PP2), attenuated EGF stimulated Rac1 activation from [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19116140
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"Basal- and EGF induced activation of Rac1 in PC3 and DU145 cells were not affected in cells after Gialpha2 knockdown."
30078221
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"The signaling of EGF activates RAC1 to control cytoskeletal reorganization, a crucial event in the metastatic cascade 19."
33633298
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"Moreover, the presence of both LY294002 and PP2 together completely blocked EGF stimulated Rac1 activation (0.8 +/- 0.3 fold-increase; Fig. 3 A and B)."
19116140
reach
"Expression of a carboxy-terminal fragment of Vav2 decreased the elevation of Rac1 activity induced by epidermal growth factor, consistent with Vav2 mediating activation of Rac1 downstream from growth factor receptors."
10982832
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"Furthermore, previous work has demonstrated that EGF stimulates the activation of Rac1 ( Marcoux and Vuori, 2003 )."
19171136
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"We therefore examined this potential mode of action, and found that EGF induced Rac1 activation was regulated by CysLT1 signaling (unpublished data)."
23139868
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"Both EGF and HGF promoted the activation of the Rac1 effector PAK in A549 cells."
34731623
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"To characterize the relationship between EGF-induced Rac1 activation and changes in VASP expression levels in MCF-7 cells, a loss-of-function approach was applied."
19171136
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"In parental A549 cells, EGF caused a time-dependent activation of Rac1 at peripheral protrusions in a PI3K-dependent manner (Figure S4D; videos in Data S1)."
34731623
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"These findings demonstrated that EGCG inhibited the invasive migration potential of EGF-induced MCF-7 cells.To further investigate the role of Rac1 and VASP in EGF-induced cells invasion and migration[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19171136
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"This result suggested that EGF‐induced Rac1 activation may occur transiently and/or locally in SKBR‐3 cells and, thus, it might be difficult to detect Rac1‐GTP from total cell lysates by the pull‐down assay."
30515933
sparser
"Ten minutes after EGF stimulation, the FRET signal became prominent at membrane ruffles at the dorsal cell surface (Figure  xref D,E; Video xref ), suggesting that EGF‐dependent activation of Rac1 occurred transiently and locally in SKBR‐3 cells."
30515933
sparser
"We therefore examined this potential mode of action, and found that EGF-induced Rac1 activation was regulated by CysLT1 signaling (unpublished data)."
23139868
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"Many signaling molecules and signal transduction pathways have been demonstrated or proposed to be involved in the EGF-induced activation of Rac1, as well as the accompanying lamellipodia formation an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19171136
reach
"Interestingly, this was consistent with our findings that the activity of Rac1 and VASP expression were stimulated by EGF in MCF-7 cells.To further study the interaction between EGF-induced Rac1 activ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19171136
reach
"While either Rac1 or VASP was silenced by siRNA, the EGF-induced invasive migration ability was sharply reduced.Finally, we evaluated the effect of EGCG on EGF-induced Rac1 activation, VASP expression[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19171136
sparser
"These results suggest that HER2 is essential for EGF‐induced Rac1 activation in SKBR‐3 cells."
30515933
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"These results showed that EGCG antagonized EGF-induced activation of Rac1 together with the concomitant up-regulation of VASP expression."
19171136
reach
"As shown in Figure 5F, depletion of Vav1 in the CAPAN2 cell line results in a defect in the basal and EGF stimulated activation of the Rac1 effector molecule Pak1."
15652748
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"EGF stimulation activated Rac1 and Rho (but not cdc42) in FG cells expressing vector control, F10-15, or F1-9 mutations (XREF_FIG)."
19208836
sparser
"Asef phosphorylation at Tyr94 plays an essential role in the EGF-induced activation of Rac1 and Cdc42 and functions in an Src-dependent manner in A431 cells [4] ."
24378532
sparser
"This EGF-induced Rac1 activation was blocked by AG1478-mediated inhibition of the EGFR ( Fig. 2 A and B)."
19116140
sparser
"Because knockdown of these GEFs suppressed EGF-induced Rac1 activation only partially, we looked for another Rac1 GEF downstream of the EGF receptor and found that Asef, a Rac1-Cdc42 GEF bound to the tumor suppressor APC, also contributed to EGF-induced Rac1 activation."
18653540
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"The suppression of EGF-induced Rac1 activation in siRNA-treated cells was restored by wild-type Asef, but not by the Tyr94Phe mutant of Asef."
18653540
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"We examined whether EGF induced changes in fibronectin and phospho-Rac1 and cdc42, which in turn increase cell migration, are mediated by COX-2 induction in HNSCC."
25595899
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"This is consistent with our results, since EGF induced activity of Rho and Rac1 in FG cells expressing the CAS F1-9 or F10-15 mutations."
19208836
sparser
"Additionally, we showed that Rac1 activation by EGF was prevented by addition of an Akt inhibitor (Figure xref )."
27102434
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"Cell based measurement of GTPase activity demonstrated that R-ketorolac specifically inhibits epidermal growth factor stimulated Rac1 and Cdc42 activation at low micromolar concentrations."
26071482
sparser
"Further investigation suggested involvement of the Eps8/Abi1/Sos1 tricomplex; we found up‐regulated expression of the Rac1 guanine nucleotide exchange factor, Sos1, in PDAC tissue and cell lines (supplementary material, Figure xref A, B), and Sos1 siRNA knockdown suppressed EGF‐induced Rac1 activation (supplementary material, Figure xref C)."
28608476
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"This is illustrated by the fact that the concentration of EGF required to cause maximal activation of Rac1 was relatively low (5 ng/ml) in the NIH3T3 and EGFR-e 3B1 cells but was much higher (at least[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16182283
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"Similar to what we observed concerning EGF induced regulation of Rac1, the two sublines differed with regard to the dose dependent effect of EGF on activation of Rap1."
16182283
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"Treatment with 100 ng/mL EGF increased RAC1, Ref-1, EGR1, and PTEN expression and markedly inhibited Akt phosphorylation in these cells."
27935858
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"Here, we confirm these findings by showing that overexpression of e3B1 in another fibroblastic cell line, NIH3T3 and EGFR, sensitizes EGF induced activation of Rac1."
16182283
sparser
"To examine the effect of PDGF-R and Src on EGF-induced Rac1 activation, VSMC were pretreated by specific PDGF-R tyrosine kinase inhibitor AG1296 or specific Src inhibitor PP1."
17350025
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"The affinity reagent PBD interacts only with the activated state of Rac1, which is induced here by epidermal growth factor (EGF) stimulation."
21858900
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"Vav2 Is Critical for EGF induced Rac1 and Cdc42 Activation, Reorganization of Junctional Actin Cytoskeleton, and Cell Migration in MECs."
20940296
reach
"Because we established that Rac1 stimulation could rescue a normal cyst phenotype for MDCK ARF6si cysts, we sought to determine whether Rac1 activation by CN02 and EGF treatment could also restore the supportive laminin-111 microenvironment."
23051733
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"Rac1 dissociation from RhoGDI and subsequent Rac1 activation induced by the growth factors EGF and PDGF required phosphorylation of S101 and S174 by Pak1."
16472651
reach
"betaPix siRNA based knockdown experiments block EGF induced H 2 O 2 production and Rac1 activation."
23639002
reach
"Recently, it has been shown to localize at the leading edge of migrating glioma cells where it promoted membrane ruffling and migration and invasion of glioma cells, as well as the EGF induced activation of Rac1."
24109588
sparser
"Overexpression of β2-chimaerin partially inhibited insulin-mediated Rac1 activation, consistent with previous reports of β2-chimaerin’s inhibitory effect on Rac1 activation by EGF or heregulin [ xref , xref , xref ]."
39598690
sparser
"Rac1 can be activated by EGF and other growth factors that elicit the exchange of GTP for the GDP bound to inactive Rac ( Dise et al., 2008 )."
18487071
sparser
"In addition, specific inhibition of Src by PP1 does not affect EGF-induced Rac1 activation."
17350025
reach
"Consistent with these results, overexpression of WT HA-Cbl diminished whereas that of HA-Cbl-C3AHN enhanced the EGF induced Rac1 activation (XREF_FIG, A and B)."
20940296
reach
"In contrast, combined Cbl and Cbl-b KD dramatically enhanced the EGF induced Vav2 phosphorylation in response to EGF stimulation (XREF_FIG G) and enhanced the EGF induced Rac1 and Cdc42 activation (XREF_FIG, A and B)."
20940296
sparser
"Therefore, we were interested to determine the effect of Src tyrosine kinase inhibitor PP1 and PDGF receptor tyrosine kinase inhibitor AG1296 on EGF-induced Rac1 activation in VSMC."
17350025
reach
"Further investigation suggested involvement of the Eps8/Abi1/Sos1 tricomplex; we found up-regulated expression of the Rac1 guanine nucleotide exchange factor, Sos1, in PDAC tissue and cell lines (supplementary material, Figure XREF_SUPPLEMENTARY A, B), and Sos1 siRNA knockdown suppressed EGF induced Rac1 activation (supplementary material, Figure XREF_SUPPLEMENTARY C)."
28608476
sparser
"U73122 treatment prevented Rac1 activation by EGF, whereas its inactive analogue did not."
22384062
reach
"Conversely, 10 ng/ml EGF (a known stimulator of both Rac1 and O (2) (-) release) increased ENaC NP (o) values in both type 1 and 2 cells."
20097736
sparser
"Altogether, these results argue for the requirement of a PLCγ-dependent activation of PKCε for the activation of Rac1 by EGF."
22384062
sparser
"PKG II Inhibits EGF-induced Activation of RAC1."
23613900
sparser
"Concurrent activation of Rac1 and Cdc42 at lamellipodia and membrane ruffles by EGF has been reported in lung cancer cells, 42,43 which was probably achieved by phosphorylation and activation of Asef[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23562274
sparser
"A second wave of EGF-induced Rac1 activation was required for EGF-induced cell migration, however, the spatiotemporal regulation of the second wave of EGF-induced Rac1 activation remains largely unclear."
24350867
sparser
"Tiam1 expression is required for the second wave of EGF-induced Rac1 activation in A431 cells."
24350867
sparser
"The present study clearly demonstrates that the activation of Rac1 by EGF plays an important role in the EGF-induced invasive phenotype in Hs578T and MDA-MB-231 cells."
21281602
reach
"Rac1 can be activated by EGF and other growth factors that elicit the exchange of GTP for the GDP bound to inactive Rac (Dise et al., 2008)."
18487071
reach
"Epidermal growth factor (EGF) stimulation substantially increases the activation of Rac1 in control P cells but essentially has no effect in activating Rac1 in cultures with 70% Ppp4 depletion."
18487071
sparser
"Published data demonstrated that EGF activated Ras [ xref ] and Rac1 [ xref ], and RAC1 regulates the expression of Smad3 [ xref ]."
38041111
sparser
"Consistent with these observations, while CD99CRIII3 suppressed the EGF-induced activation of Rac1 and RhoA GTPases, its inhibitory effect was neutralized by siRNA-mediated knockdown of PTPN12 in MCF-7 cells ( xref E)."
33050232
sparser
"Recently, Arnst et al ( xref ) demonstrated two new compounds, referred in their publication as #1 and #6, that were design to target the nucleotide-binding site of Rac1 although, were able to block active Rac1 from binding to its effector PAK1, following EGF-induced Rac1 activation in a dose-dependent manner, they showed no inhibition of Cdc42 or RhoA."
28848995
reach
"Epithelial growth factor (EGF)-mediated activation of Rho GTPase Rac1 and formation of lamellipodia in TS/A cells were remarkably suppressed by DET treatment."
22342517
sparser
"The effects of Rac1 activation by EGF, and deactivation by Rac1 inhibitor NSC23766, in OECM1 & SAS spheroids are given in Fig. S4 ."
30500449
sparser
"To assess the involvement of Rac1 in the EGF-induced invasive phenotype of Hs578T and MDA-MB-231 cells, we first examined if EGF activated Rac1 in these cells."
21281602
sparser
"The level of the active form of Rac1(Rac1-GTP) decreased by the knock-down of H-Ras using siRNA targeting H-Ras, demonstrating that activation of Rac1 by EGF was dependent on H-Ras in Hs578T and MDA-M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21281602
reach
"In this study, we found that EGF treatment could activate Ras related C3 botulinum toxin substrate 1 (Rac1), PI3K and Akt and p21 actived kinase (PAK1) along with cell migration."
23554696
sparser
"We previously found that the EGF-induced biphasic activation of Rac1 and the second-wave Rac1 activation require the upregulation of Tiam1, a member of the Rac GEF family. xref We also demonstrated that CysLT1 signaling played an important role in Tiam1 upregulation required for the second EGF-induced wave of Rac1 activation."
24350867
sparser
"FIP-fve inhibited EGF-induced activation of Rac1."
24274472
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"The ruffling observed in SNV- treated cells is similar to that seen in response to EGF mediated activation of Rac1 dependent ruffling [XREF_BIBR]."
23928044
sparser
"Finally, we evaluated the effect of EGCG on EGF-induced Rac1 activation, VASP expression, and MCF-7 cell invasion and migration."
19171136
sparser
"These results showed that EGCG antagonized EGF-induced activation of Rac1 together with the concomitant up-regulation of VASP expression."
19171136
reach
"Although LTC4/D4/E4 exert their effects by binding to their receptors, cysteinyl leukotriene receptor 1 (CysLT1), CysLT1 specific antagonists MK-571 [23] and montelukast [24] or knockdown of CysLT1 by siRNA were found to inhibit the EGF induced second wave, but not the first wave of Rac1 activation."
30247093
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"Because of the fast activation of RhoG and Rac1 by EGF treatment (30 s) and to separate these events from later (potentially secondary) activation of these GTPases, we refer from here on to their activation at 30 s as the " rapid activation. ""
20237158
sparser
"To characterize the relationship between EGF-induced Rac1 activation and changes in VASP expression levels in MCF-7 cells, a loss-of-function approach was applied."
19171136
sparser
"Many signaling molecules and signal transduction pathways have been demonstrated or proposed to be involved in the EGF-induced activation of Rac1, as well as the accompanying lamellipodia formation an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19171136
sparser
"To further study the interaction between EGF-induced Rac1 activation and the change in VASP expression, a loss-of-function approach using siRNAs for VASP and Rac1 was applied."
19171136
sparser
"Protein phosphatase 1B also dephosphorylates RhoGDI1 and suppresses epidermal growth factor-induced activation of RhoA, Rac1, and Cdc42 [ xref ]."
38355643
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"In addition, EGF signaling through PAK controls the extent of macropinocytosis in these cells ( Lee et al., 2019c ) and stimulates Rac1, PAK, and JNK in vascular smooth muscle cells ( Beier et al., 20[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34390849
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"Furthermore, specific downregulation of PI3K and Akt signaling in MDA-MB-231 cells did not alter EGF induced activation of Rac1."
23554696
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"EGF and TGFalpha, but not Fnf, increased Rac1 activity and promoted the increase in MMP-13 above that stimulated by Fnf alone."
23460186
sparser
"EGF-dependent Rac1 activation is regulated by endocytosis."
39744818
reach
"Dock4 and HIF2A knockout in MDCK cells reduce EGF-induced activation of Rac1/Pak1-signaling."
38802496
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"In cells clamped at pH 7.8, both Rac1 and Cdc42 were stimulated by EGF (XREF_FIG), as found earlier."
20156964
reach
"Treatment of control MDCK cells with ZINC69391 inhibited EGF-induced Rac1 activity (Fig. S5a–c) leading to decreased Pak1 phosphorylation (Fig. S5d,e)."
38802496
sparser
"The requirement of CME for sustained VAV2-dependent Rac1 activation by EGF prompted us to test whether Rac1 is present in EGFR-containing endosomes in HSC3 cells."
39744818
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"Thus, the FRET analysis is consistent with the biochemical data, indicating that Rac1 and to a lesser extent Cdc42 are activated by EGF and that both GTPases are sensitive to moderate cytosolic acidification."
20156964
reach
"The preceding results indicate that Rac1 and Cdc42 are stimulated by EGF, but do not directly link their activity to ruffling and macropinocytosis."
20156964
sparser
"In HSC3 cells, our data indicate that VAV2 is essential for EGF-induced Rac1 activation ( xref ; xref ) and has a major role in EGF-guided chemotactic cell migration ( xref )."
39744818
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"In a published report, compound ZINC69391 was found to block Rac1 interaction with Tiam1 (one of the Rho GEFs for Rac), thereby preventing the EGF-induced Rac1 activation."
32006571
sparser
"Consequently, we examined whether ILK/ELMO2 species can modulate EGF-induced Rac1 activation in epidermal keratinocytes."
22160594
sparser
"Exogenous expression of ILK and/or ELMO2 significantly enhanced EGF-induced Rac1 activation over the same period ( xref and xref )."
22160594
sparser
"The EGF-induced activation of Rac1 in cells exogenously expressing ILK and ELMO2 suggests Rac1 may mediate ILK/ELMO2 modulation of cell polarization."
22160594
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"Because the PI3K and Akt signaling pathway plays a vital role in EGF induced migration by activating Rac1 and Cdc42, that are important for lamellipodia and filopodia formation respectively, and found upregulated in metastatic lesions, we further examined the activation status of Rac1 and Cdc42 in the absence or presence of EGF by G-LISA."
27167206
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"Even though no study has attempted to analyze comprehensively all these GEFs simultaneously and their interplay, it seems likely that Rac1 activation by EGF is controlled in a cell type- and cell function dependent manner through these different GEFs."
20237158
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"We showed that in A431 cells PLEKHG6 together with Vav family GEFs is involved in the rapid activation of RhoG after EGF stimulation (XREF_FIG, D and E), whereas EGF induced Rac1 activity is not influenced by this GEF."
20237158
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"20 Additionally, research has demonstrated that mevastatin facilitates wound healing by mediating EGF-induced Rac1 activation, leading to the migration phenotype of primary human keratinocytes, actin cytoskeleton reorganization, and lamellipodia formation."
39720696
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"3.4 EGF induced Rac1 activation is abolished by RV treatment."
21732534
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"XREF_FIG shows, that 20 ng/mL EGF caused a 2.4-fold Rac1 activation after 5 min that was completely inhibited by preincubation with 50 muM RV for 30 min."
21732534
reach
"More recently, Chang et al. demonstrated that in addition to PVF and EGF driven upregulation of Rac1, the formation of polarized protrusions in the leader cell is supported by negative regulation of the Hippo pathway by Rap1 [XREF_BIBR]."
31404788
reach
"Rac1 activation was strongly enhanced by EGF treatment both in the presence as well as in the absence of CaM."
33220265
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"This unexpected result suggests that EGF may induce a CaM-insensitive state of Rac1, explaining why EGF-dependent 2D-cell migration was not inhibited upon CaM downregulation, in contrast to the observ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33220265
reach
"Previous studies have shown that the knockdown of Galpha i 2 attenuates cell migration without causing any reduction in basal Rac1 activity in both PC3 and DU145 cells, and has only marginal effects on the epidermal growth facotor (EGF)-induced increase in Rac1 activity."
40141305
reach
"PKG II Inhibits EGF induced Activation of RAC1."
23613900
reach
"The result showed that EGF treatment caused an obvious increase of active RAC1 and high activity of PKG II efficiently inhibited the activation of RAC1 (XREF_FIG)."
23613900
sparser
"MCF-7 cells were stimulated with EGF and IGF-I, which activate Rac1 in a P-Rex1-dependent manner [ xref ], as well as with 10% FBS."
29983884
sparser
"Cell migration is driven by the second wave of EGF-induced Rac1 activation."
30544910
sparser
"It was further shown that EGF activates Rac1 through the activation of Tiam1, either via 14-3-3ζ or through 5-lipoxygenase-leukotriene C4 [ xref , xref ]."
30544910
reach
"While EGF also activates Rac1, this effect is rapid and short lived."
21893191
sparser
"UTKO1 inhibited EGF-induced Rac1 activation at 12 hrs with the same concentration to inhibit lamellipodia formation."
30247093
sparser
"In a published report, compound ZINC69391 was found to block Rac1 interaction with Tiam1 (one of the Rho GEFs for Rac), thereby preventing the EGF-induced Rac1 activation."
32006571
sparser
"Down-regulation of Asef by siRNA inhibits the EGF-induced activation of Rac1."
30544910
reach
"Moreover, silencing of ARF6 decreased by six-fold the level of GTP : Rac1 both in non stimulated condition and in response to EGF stimulation and abolished EGF induced Rac1 activation (XREF_FIG)."
25799492
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"Depletion of ARF1 significantly inhibited EGF dependent activation of Rac1."
23707487
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"In conditions where cells were depleted of ARF1, Rac1 and IRSp53 remained in the cytosol even when cells were treated with EGF."
23707487
sparser
"We showed that inhibition of membrane-localized ERK activity potentiates EGF-induced Rac1 activation and leads to enhanced membrane protrusion dynamics."
32452765
reach
"Additionally, we showed that Rac1 activation by EGF was prevented by addition of an Akt inhibitor."
27102434
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"These results indicate that DGKγ inhibits EGF-induced Rac1 activation through enhancing β2-chimaerin Rac-GAP activity in vivo.To determine whether the regulation of β2-chimaerin Rac-GAP activity by DG[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17254573
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"An extensive functional analysis using P-Rex1 overexpressing breast cancer cell lines established the requirement of this GEF for Rac1 activation and cell migration induced by EGF, TGFalpha or HRG."
21893191
reach
"R-naproxen and R-ketorolac inhibit serum and EGF-stimulated Rac1 and Cdc42 activation and downstream signaling through a proposed allosteric mechanism [48,95]."
30261690
sparser
"Recently, it has been shown to localize at the leading edge of migrating glioma cells where it promoted membrane ruffling and migration and invasion of glioma cells, as well as the EGF-induced activation of Rac1 ( xref )."
24109588
sparser
"Thus, while expression of constitutively activated Rac1 can act to suppress interdigitation ( Figure S3 ), the rapid reversal process appears to be largely independent of the major pool of EGF-activat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25242328
sparser
"However, further Rac1 activation by EGF was absent in CdGAP KD podocytes (Fig.  xref D,E)."
36333327
reach
"To assess the involvement of Rac1 in the EGF induced invasive phenotype of Hs578T and MDA-MB-231 cells, we first examined if EGF activated Rac1 in these cells."
21281602
reach
"The level of the active form of Rac1 (Rac1-GTP) decreased by the knock-down of H-Ras using siRNA targeting H-Ras, demonstrating that activation of Rac1 by EGF was dependent on H-Ras in Hs578T and MDA-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21281602
reach
"Furthermore, inhibition of EGF-R by tyrphostin AG1478 attenuates EGF-induced stimulation of Rac1, PAK and JNK."
17350025
sparser
"In the present study, we demonstrate that MEK activation by EGF increased Rac1 activation, dissociation of intercellular contacts, and migration in both control and polyamine-depleted cells, while U0126, a specific inhibitor of MEK1, prevented disruption of junctions as well as EGF-induced Rac1 activation."
17183546
reach
"The present study clearly demonstrates that the activation of Rac1 by EGF plays an important role in the EGF induced invasive phenotype in Hs578T and MDA-MB-231 cells."
21281602
reach
"S100A7 overexpression affect EGF induced Rac1 activation and associated signaling."
23535840
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"The dependence of EGF-stimulated Rac1 binding was determined at 1 min."
17350025
reach
"Specific inhibition of EGF-R by AG1478, or tyrosine kinase inhibition by genistein, attenuated EGF-induced activation of Rac1 ( Fig. 3 A ), PAK ( Fig. 3 B) and JNK ( Fig. 3 C)."
17350025
reach
"To examine the effect of PDGF-R and Src on EGF-induced Rac1 activation, VSMC were pretreated by specific PDGF-R tyrosine kinase inhibitor AG1296 or specific Src inhibitor PP1."
17350025
reach
"The results demonstrated that CHAG treatment efficiently blocked the phosphorylation and activation of EGFR, integrin and VEGFR, inhibited the EGF induced signaling of MAPK and ERK, PI3K and Akt and Rac1 mediated pathways, and diminished the EGF induced expression of proliferation and migration related proteins."
27589842
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"Neither inhibition of PDGF-R, nor inhibition of Src affected EGF-mediated stimulation of Rac1 in VSMC ( Fig. 4 A and B )."
17350025
reach
"To assess the functional effects of EGF-mediated Rac1 activation in VSMC, we pretreated growth arrested VSMC for 12 h with the specific Rac1 inhibitor NSC23766 and determined EGF-induced DNA synthesis[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17350025
reach
"In the present study, we report that EGF stimulates the GTPase Rac1 in VSMC."
17350025
reach
"In addition, specific inhibition of Src by PP1 does not affect EGF-induced Rac1 activation."
17350025
reach
"Therefore, we were interested to determine the effect of Src tyrosine kinase inhibitor PP1 and PDGF receptor tyrosine kinase inhibitor AG1296 on EGF-induced Rac1 activation in VSMC."
17350025
reach
"However, we could not detect any effect of Src- or PDGF-R inhibition on EGF-induced PAK activation, indicating that the endogenous EGF receptor tyrosine kinase is sufficient for Rac activation in VSMC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17350025
reach
"FIP-gmi had little effect on the EGF induced activation of Rac1 GTPase.Promotion of lymphocyte proliferation The lymphocyte is a kind of leucocyte produced by the lymphoid organs, which is an important functioning component in the immune response."
21651437