IndraLab
Statements
reach
"RNA (2 ml) was added to 23 l of PCR mix in each well of a MicroAmp optical reaction plate containing 12.5 l of Taqman One-Step PCR Mastermix, 0.625 l of 40× Multiscribe/RNase inhibitor, 5.75 l of distilled water, 1.25 l each of 18 M NiV or HeV forward and reverse primers, 1.25 l of 5 M HeV or NiV FAM-labeled probe, 0.125 l each of 10 M 18S rRNAF and 18S rRNAR, and 0.125 l of 40 M 18S rRNA-VIClabeled probe."